Rmu_sc0001362.1_g000008

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001362.1
N/A
Physical Location & Seq
Forward (+)
21210 .. 24596
3387 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1539 bp
atggagatggagagtttgaacgttggacttgaatcttggaagcaagctttaactcttcacgacttcaatgatggatggtggatgctagcctcacttcactccgccaagcagccctcgccctgtaagctaaactcgctagctgataagctcgcctcacctctacacaagctagattggcctcgacagcagcctgccctacctcccccgcgcacaccagcctgctgcccagcaccactgtacctttggttagcctcgctagccttcgtggagtccaccaccttggtgcaagtcatccaaacctatgcaaaaaccaagcagctaaacaaaggcaaagagctccacgctcacctcctacgcacccattacccactatgcattttcctcaccaaccacctcctcaaaatgtactccaaatgcgggcacgtcgactatgccctcaaggttttcgaccaaatgcctcaccaaaacttggtttcttggattgcaatggtaactgggttttctcagaacttgaggttatcggagagtttcaaggccttttcccatatgagaagtactggggagagcccgaaccagtttgcattttcaagtgtcatcagagcttgtgtggttctttgtaacttggcggatatgtattcgaaatgtgggttcatggttgatgcttgtaaggttttcgaggaaataccgagtaaggatgttgtgtcatggacttcgatgattgatgggcttgtcagtttggcattggaagtagaggttgctgtgggaaatgctctggttgatatgtactcaaaatcaagagatatggagagtgccttacatgtgtttcgagggggtggatgtggggtgagagttaagcctaatcagttcactttttctagcttggtcaaggcttgtaccaaccaagctgcacttgaacaagggattcaacttcatgctcaggtggtcaagtttaattttgatagagacaattttgtgtcttgtgttcttgttgatatgtatggtaaatgtgggttacttgatcattcaatacaagtgtttgacgaggccaccccgactgaagttgcatggaattcattgctaagtgtatttgcagttcatggcttaggaaatgacaccttgaaaacttttaatagaatggtccaggcaggagtgaaaccaaatgcaataacatttatcaagctcgacattactcttgcattattgatttacttggccgggctggaaggcgtgaagaggccgaagattttattaacagcatgtgaatccagccagatacttttgggtggtgcttgcacaattcatggtgataaagaggggggggacaaattggcggagaaattgatacagtttgaacctgagaatagtggagcagatgttttgctttcaactctacatgcaaataagcaaaaatgggaagatctcagaagtgtgaggaagatgatgagagacagcagcatgaagaaatttcccggttatagttgggttgatgttggaaacaaaacacacacgtctggagccgaagattggtgtcatccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

512

Amino Acids

56.88

Weight (kDa)

7.55

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 156 - 202 4.4e-06 PPR repeat family
PPR_2 PF13041 351 - 391 1.4e-07 PPR repeat family
E_motif PF20431 434 - 496 8e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 469
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 208
AciI CCGC 5 cut(s) 102, 206, 417, 626, 1331
AclI AACGTT 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 1212
AcsI RAATTY 2 cut(s) 1069, 1463
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 5 cut(s) 239, 407, 556, 785, 895
AfiI CCNNNNNNNGG 3 cut(s) 417, 469, 1524
AflIII ACRYGT 2 cut(s) 817, 1506
AjiI CACGTC 2 cut(s) 424, 1509
AjnI CCWGG 1 cut(s) 1140
AleI CACNNNNGTG 1 cut(s) 281
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 957, 1440
AoxI GGCC 5 cut(s) 176, 534, 1044, 1212, 1235
ApeKI GCWGC 6 cut(s) 109, 187, 222, 316, 905, 1452
ApoI RAATTY 2 cut(s) 1069, 1463
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 1138
AsuC2I CCSGG 2 cut(s) 1216, 1470
AsuHPI GGTGA 6 cut(s) 147, 338, 376, 452, 856, 1316
AsuII TTCGAA 1 cut(s) 638
AsuNHI GCTAGC 3 cut(s) 85, 136, 256
AvaII GGWCC 1 cut(s) 1138
BaeGI GKGCMC 1 cut(s) 423
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BanII GRGCYC 2 cut(s) 339, 569
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 6 cut(s) 121, 199, 209, 328, 892, 1464
BccI CCATC 3 cut(s) 65, 69, 716
BciT130I CCWGG 1 cut(s) 1142
BclI TGATCA 1 cut(s) 1018
BcnI CCSGG 2 cut(s) 1216, 1470
BcoDI GTCTC 2 cut(s) 957, 1440
BfaI CTAG 5 cut(s) 86, 137, 170, 257, 876
BglII AGATCT 1 cut(s) 1417
BisI GCNGC 6 cut(s) 110, 188, 223, 317, 906, 1453
BlsI GCNGC 6 cut(s) 111, 189, 224, 318, 907, 1454
BmcAI AGTACT 1 cut(s) 556
Bme1390I CCNGG 3 cut(s) 1142, 1216, 1470
Bme18I GGWCC 1 cut(s) 1138
BmgBI CACGTC 2 cut(s) 424, 1509
BmgT120I GGNCC 1 cut(s) 1138
BmiI GGNNCC 1 cut(s) 1516
BmrFI CCNGG 3 cut(s) 1142, 1216, 1470
BmrI ACTGGG 2 cut(s) 504, 567
BmsI GCATC 2 cut(s) 72, 649
BmtI GCTAGC 3 cut(s) 89, 140, 260
BmuI ACTGGG 2 cut(s) 504, 567
BpmI CTGGAG 1 cut(s) 1533
Bpu10I CCTNAGC 2 cut(s) 936, 1102
Bpu14I TTCGAA 1 cut(s) 638
BpuEI CTTGAG 2 cut(s) 422, 532
BpuMI CCSGG 2 cut(s) 1216, 1470
BsaJI CCNNGG 1 cut(s) 279
BsaXI ACNNNNNCTCC 2 cut(s) 1359, 1389
Bsc4I CCNNNNNNNGG 3 cut(s) 417, 469, 1524
Bse1I ACTGG 3 cut(s) 499, 562, 574
Bse3DI GCAATG 2 cut(s) 492, 1073
BseBI CCWGG 1 cut(s) 1142
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 6 cut(s) 80, 87, 291, 700, 842, 1531
BseLI CCNNNNNNNGG 3 cut(s) 417, 469, 1524
BseMI GCAATG 2 cut(s) 492, 1073
BseMII CTCAG 4 cut(s) 518, 950, 1347, 1435
BseNI ACTGG 3 cut(s) 499, 562, 574
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 6 cut(s) 121, 199, 209, 328, 892, 1464
BseYI CCCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 891
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 5 cut(s) 178, 536, 1046, 1214, 1237
BsiHKAI GWGCWC 1 cut(s) 339
BsiSI CCGG 2 cut(s) 1215, 1470
BslFI GGGAC 1 cut(s) 1334
BslI CCNNNNNNNGG 3 cut(s) 417, 469, 1524
BsmAI GTCTC 2 cut(s) 957, 1440
BsmFI GGGAC 1 cut(s) 1334
BsnI GGCC 5 cut(s) 178, 536, 1046, 1214, 1237
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 3 cut(s) 339, 423, 569
Bsp143I GATC 2 cut(s) 1018, 1417
BspACI CCGC 5 cut(s) 102, 206, 417, 626, 1331
BspANI GGCC 5 cut(s) 178, 536, 1046, 1214, 1237
BspCNI CTCAG 4 cut(s) 517, 949, 1348, 1434
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 1516
BspOI GCTAGC 3 cut(s) 89, 140, 260
BspT104I TTCGAA 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 492, 1073
BsrI ACTGG 3 cut(s) 499, 562, 574
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 1018, 1417
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 1142
Bst4CI ACNGT 2 cut(s) 237, 1347
Bst6I CTCTTC 2 cut(s) 60, 1226
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 9 cut(s) 45, 87, 138, 150, 192, 220, 258, 419, 1291
BstDEI CTNAG 6 cut(s) 504, 936, 1079, 1102, 1356, 1421
BstF5I GGATG 6 cut(s) 80, 87, 291, 700, 842, 1531
BstFNI CGCG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 2 cut(s) 1021, 1420
BstMAI GTCTC 2 cut(s) 957, 1440
BstMBI GATC 2 cut(s) 1018, 1417
BstMWI GCNNNNNNNGC 6 cut(s) 115, 124, 133, 175, 184, 257
BstNI CCWGG 1 cut(s) 1142
BstNSI RCATGY 3 cut(s) 821, 1260, 1397
BstSCI CCNGG 3 cut(s) 1140, 1214, 1468
BstSLI GKGCMC 1 cut(s) 423
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 6 cut(s) 121, 199, 209, 328, 892, 1464
BstX2I RGATCY 1 cut(s) 1417
BstXI CCANNNNNNTGG 1 cut(s) 280
BstYI RGATCY 1 cut(s) 1417
BsuRI GGCC 5 cut(s) 178, 536, 1046, 1214, 1237
BtrI CACGTC 2 cut(s) 424, 1509
BtsCI GGATG 6 cut(s) 80, 87, 291, 700, 842, 1531
BtsIMutI CAGTG 1 cut(s) 233
Cac8I GCNNGC 9 cut(s) 45, 87, 138, 150, 192, 220, 258, 419, 1291
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 1138
Csp6I GTAC 5 cut(s) 238, 406, 555, 784, 894
CviQI GTAC 5 cut(s) 238, 406, 555, 784, 894
DdeI CTNAG 6 cut(s) 504, 936, 1079, 1102, 1356, 1421
DpnI GATC 2 cut(s) 1020, 1419
DpnII GATC 2 cut(s) 1018, 1417
EaeI YGGCCR 1 cut(s) 1212
Eam1104I CTCTTC 2 cut(s) 60, 1226
EarI CTCTTC 2 cut(s) 60, 1226
EciI GGCGGA 3 cut(s) 91, 641, 1346
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 279
Eco147I AGGCCT 1 cut(s) 536
Eco24I GRGCYC 2 cut(s) 339, 569
Eco47I GGWCC 1 cut(s) 1138
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 1077
EcoICRI GAGCTC 1 cut(s) 337
EcoRI GAATTC 1 cut(s) 1069
EcoRII CCWGG 1 cut(s) 1140
EcoT14I CCWWGG 1 cut(s) 279
EcoT22I ATGCAT 1 cut(s) 377
EcoT38I GRGCYC 2 cut(s) 339, 569
ErhI CCWWGG 1 cut(s) 279
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 1 cut(s) 1334
FauI CCCGC 2 cut(s) 213, 410
FauNDI CATATG 1 cut(s) 546
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 6 cut(s) 110, 188, 223, 317, 906, 1453
FokI GGATG 6 cut(s) 87, 94, 278, 707, 849, 1518
FriOI GRGCYC 2 cut(s) 339, 569
Fsp4HI GCNGC 6 cut(s) 110, 188, 223, 317, 906, 1453
FspBI CTAG 5 cut(s) 86, 137, 170, 257, 876
GlaI GCGC 1 cut(s) 209
GluI GCNGC 6 cut(s) 110, 188, 223, 317, 906, 1453
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 1533
HaeIII GGCC 5 cut(s) 178, 536, 1046, 1214, 1237
HapII CCGG 2 cut(s) 1215, 1470
HhaI GCGC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 4 cut(s) 32, 269, 922, 1262
HpaII CCGG 2 cut(s) 1215, 1470
HphI GGTGA 6 cut(s) 147, 338, 376, 452, 856, 1316
Hpy166II GTNNAC 3 cut(s) 273, 427, 867
Hpy188I TCNGA 4 cut(s) 507, 523, 599, 1424
Hpy188III TCNNGA 3 cut(s) 59, 795, 1512
Hpy8I GTNNAC 3 cut(s) 273, 427, 867
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 2 cut(s) 271, 1217
HpyCH4III ACNGT 2 cut(s) 237, 1347
HpyCH4IV ACGT 3 cut(s) 21, 423, 1508
HpyF10VI GCNNNNNNNGC 6 cut(s) 115, 124, 133, 175, 184, 257
HpyF3I CTNAG 6 cut(s) 504, 936, 1079, 1102, 1356, 1421
HpySE526I ACGT 3 cut(s) 21, 423, 1508
HspAI GCGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 1018
Kzo9I GATC 2 cut(s) 1018, 1417
LmnI GCTCC 3 cut(s) 342, 1367, 1514
Lsp1109I GCAGC 6 cut(s) 121, 199, 209, 328, 892, 1464
LweI GCATC 2 cut(s) 72, 649
MaeI CTAG 5 cut(s) 86, 137, 170, 257, 876
MaeII ACGT 3 cut(s) 21, 423, 1508
MaeIII GTNAC 3 cut(s) 490, 617, 1011
MalI GATC 2 cut(s) 1020, 1419
MboI GATC 2 cut(s) 1018, 1417
MboII GAAGA 7 cut(s) 47, 1243, 1252, 1427, 1447, 1471, 1532
MflI RGATCY 1 cut(s) 1417
MhlI GDGCHC 3 cut(s) 339, 423, 569
MluCI AATT 7 cut(s) 952, 967, 1069, 1296, 1325, 1337, 1463
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 2 cut(s) 4, 1471
Mph1103I ATGCAT 1 cut(s) 377
MseI TTAA 5 cut(s) 50, 852, 951, 1128, 1250
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 2 cut(s) 1215, 1470
MspR9I CCNGG 3 cut(s) 1142, 1216, 1470
MvaI CCWGG 1 cut(s) 1142
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 6 cut(s) 115, 124, 133, 175, 184, 257
NciI CCSGG 2 cut(s) 1216, 1470
NdeI CATATG 1 cut(s) 546
NdeII GATC 2 cut(s) 1018, 1417
NheI GCTAGC 3 cut(s) 85, 136, 256
NlaIV GGNNCC 1 cut(s) 1516
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 3 cut(s) 821, 1260, 1397
NspV TTCGAA 1 cut(s) 638
OliI CACNNNNGTG 1 cut(s) 281
PceI AGGCCT 1 cut(s) 536
PciI ACATGT 1 cut(s) 817
PfeI GAWTC 3 cut(s) 32, 922, 1262
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 6 cut(s) 111, 189, 224, 318, 907, 1454
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PscI ACATGT 1 cut(s) 817
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 1140
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 1140
PspN4I GGNNCC 1 cut(s) 1516
PspPI GGNCC 1 cut(s) 1138
PsrI GAACNNNNNNTAC 2 cut(s) 1077, 1109
PsuI RGATCY 1 cut(s) 1417
RsaI GTAC 5 cut(s) 239, 407, 556, 785, 895
RsaNI GTAC 5 cut(s) 238, 406, 555, 784, 894
RseI CAYNNNNRTG 1 cut(s) 281
SacI GAGCTC 1 cut(s) 339
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 5 cut(s) 50, 852, 951, 1128, 1250
SatI GCNGC 6 cut(s) 110, 188, 223, 317, 906, 1453
Sau3AI GATC 2 cut(s) 1018, 1417
Sau96I GGNCC 1 cut(s) 1138
ScaI AGTACT 1 cut(s) 556
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 3 cut(s) 1142, 1216, 1470
SduI GDGCHC 3 cut(s) 339, 423, 569
SfaNI GCATC 2 cut(s) 72, 649
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 1 cut(s) 1138
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 2 cut(s) 437, 511
SmoI CTYRAG 2 cut(s) 437, 511
Sse9I AATT 7 cut(s) 952, 967, 1069, 1296, 1325, 1337, 1463
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 5 cut(s) 102, 206, 417, 626, 1331
SspMI CTAG 5 cut(s) 86, 137, 170, 257, 876
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 3 cut(s) 1140, 1214, 1468
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 237, 1347
TaiI ACGT 3 cut(s) 24, 426, 1511
TaqI TCGA 8 cut(s) 181, 426, 447, 638, 675, 713, 826, 1182
TasI AATT 7 cut(s) 952, 967, 1069, 1296, 1325, 1337, 1463
TatI WGTACW 3 cut(s) 405, 554, 783
TfiI GAWTC 3 cut(s) 32, 922, 1262
Tru1I TTAA 5 cut(s) 50, 852, 951, 1128, 1250
Tru9I TTAA 5 cut(s) 50, 852, 951, 1128, 1250
TscAI CASTG 1 cut(s) 240
TseI GCWGC 6 cut(s) 109, 187, 222, 316, 905, 1452
TspDTI ATGAA 6 cut(s) 640, 920, 1062, 1085, 1289, 1472
TspRI CASTG 1 cut(s) 240
Van91I CCANNNNNTGG 1 cut(s) 469
VpaK11BI GGWCC 1 cut(s) 1138
XapI RAATTY 2 cut(s) 1069, 1463
XceI RCATGY 3 cut(s) 821, 1260, 1397
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 426
XspI CTAG 5 cut(s) 86, 137, 170, 257, 876
ZrmI AGTACT 1 cut(s) 556
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.