Rmu_sc0001400.1_g000012

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001400.1
N/A
Physical Location & Seq
Forward (+)
49821 .. 59085
9265 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1221 bp
atgggccggtccgagcccgagtccgataagaaatgggccgacatggaccaggtatacgatgctgttgttggagatacgacaatcacatcaaacagatcgttatatgttgattttacagtaccatattctgacttgggcttgggaatgctagtagcaaatgaaaaggaaaacatgtggattttcttaagacctctttcagcagatctatggatgacaagtgctggtttctttatcctaactggttttgttgtgtgggtaattgagcgtcctacaaacacagaattccagggcacattatcacagcaaattggaacaatattttggttctccttttcaacccttgtgtttgctcatatggggctaaaccatatcgttcggttgaagcatatgcgcgctgatgctatatcaagagggagtaagcatggtggtgttgctgctattgttgacgaggtaccatacattaaggtcttccttgcaaagtattatgagggctactccatgattaaaaccgatcagtctactaccaatggttttggctttgctttccctaaaggcttgaaattggcacaggacatgtcaacgcaaattgaaaaattgagagaagaaggaaagcttttagagatggaaaaggcctggtttcataatggaaatcacatgtttgaggacacaacaagtcctcccaatactcttcatctcagcagtttttgtggtgtattccttgctactggggtttcaccacctaacttggtggttgacagcgaaggcgccgcccatctgtggcggtggtttccggcaacttccggacgcgacagtcgccggcaacgcatcccgacttcagcactctcaggtgatatggcaactgtaaggaatctgaaaatcaagactgggacttgtaaacgcctcgttaaggagtttcattcttatgagaaagagattgagagagaggctgcaaaaacagcagacatgaaggggaaaggagctgatccttatgacctcaagcaacaggaaaatgtgctggctgaatcaaggatgatgattcccgattgtcgaaagcgcctggaggcctcactagctgactctcttgcccagtttgttaagcaatgtatttatgatcaggctgagttggaagaagagttgagccagaaggaaggccctgaaattgaagaagctcagactattattgcagaagttgataagttgttccagacaacggaagcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan Lig_chan-Glu_bd SBP_bac_3

Protein Analysis

406

Amino Acids

45.74

Weight (kDa)

5.43

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP_bac_3 PF00497 19 - 214 5.4e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 67 - 117 9.3e-09 Ligand-gated ion channel
TBCA PF02970 287 - 375 5.1e-26 Tubulin binding cofactor A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 810
Acc65I GGTACC 1 cut(s) 451
AccB1I GGYRCC 2 cut(s) 451, 764
AccI GTMKAC 2 cut(s) 54, 518
AccII CGCG 2 cut(s) 393, 807
AccIII TCCGGA 1 cut(s) 800
AciI CCGC 2 cut(s) 768, 781
AclWI GGATC 1 cut(s) 977
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 819
AcyI GRCGYC 1 cut(s) 765
AfaI GTAC 2 cut(s) 120, 453
AfiI CCNNNNNNNGG 3 cut(s) 777, 907, 1210
AflII CTTAAG 1 cut(s) 184
AflIII ACRYGT 3 cut(s) 171, 573, 654
AgsI TTSAA 5 cut(s) 336, 382, 559, 590, 1163
AjnI CCWGG 4 cut(s) 48, 285, 632, 1056
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 4 cut(s) 613, 980, 1073, 1169
AluI AGCT 4 cut(s) 613, 980, 1073, 1169
AlwI GGATC 1 cut(s) 977
Ama87I CYCGRG 1 cut(s) 17
Aor13HI TCCGGA 1 cut(s) 800
AoxI GGCC 5 cut(s) 4, 36, 630, 1062, 1150
ApeKI GCWGC 2 cut(s) 434, 947
ApoI RAATTY 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 451
AspLEI GCGC 4 cut(s) 393, 395, 767, 1056
AspS9I GGNCC 5 cut(s) 4, 9, 36, 46, 1151
AsuHPI GGTGA 2 cut(s) 726, 860
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 2 cut(s) 9, 46
BaeGI GKGCMC 1 cut(s) 293
BanI GGYRCC 2 cut(s) 451, 764
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 460
BbvI GCAGC 2 cut(s) 421, 934
BccI CCATC 2 cut(s) 616, 780
BciT130I CCWGG 4 cut(s) 50, 287, 634, 1058
BclI TGATCA 1 cut(s) 1111
BfaI CTAG 3 cut(s) 149, 1070, 1219
BfoI RGCGCY 2 cut(s) 768, 1057
BfrI CTTAAG 1 cut(s) 184
BglII AGATCT 1 cut(s) 202
BisI GCNGC 3 cut(s) 435, 768, 948
BlsI GCNGC 3 cut(s) 436, 769, 949
Bme1390I CCNGG 4 cut(s) 50, 287, 634, 1058
Bme18I GGWCC 2 cut(s) 9, 46
BmeT110I CYCGRG 1 cut(s) 17
BmgT120I GGNCC 5 cut(s) 4, 9, 36, 46, 1151
BmiI GGNNCC 2 cut(s) 453, 766
BmrFI CCNGG 4 cut(s) 50, 287, 634, 1058
BmrI ACTGGG 3 cut(s) 735, 894, 1081
BmsI GCATC 3 cut(s) 49, 388, 834
BmuI ACTGGG 3 cut(s) 735, 894, 1081
BpiI GAAGAC 1 cut(s) 460
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 1 cut(s) 1079
BpuEI CTTGAG 1 cut(s) 980
BsaHI GRCGYC 1 cut(s) 765
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 800
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 3 cut(s) 777, 907, 1210
Bse118I RCCGGY 2 cut(s) 6, 816
Bse1I ACTGG 4 cut(s) 244, 730, 889, 1087
Bse3DI GCAATG 1 cut(s) 1106
BseAI TCCGGA 1 cut(s) 800
BseBI CCWGG 4 cut(s) 50, 287, 634, 1058
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 3 cut(s) 216, 825, 1035
BseLI CCNNNNNNNGG 3 cut(s) 777, 907, 1210
BseMI GCAATG 1 cut(s) 1106
BseMII CTCAG 4 cut(s) 709, 858, 1110, 1184
BseNI ACTGG 4 cut(s) 244, 730, 889, 1087
BsePI GCGCGC 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 2 cut(s) 421, 934
Bsh1236I CGCG 2 cut(s) 393, 807
BshFI GGCC 5 cut(s) 6, 38, 632, 1064, 1152
BshNI GGYRCC 2 cut(s) 451, 764
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 4 cut(s) 7, 791, 801, 817
BslFI GGGAC 1 cut(s) 901
BslI CCNNNNNNNGG 3 cut(s) 777, 907, 1210
BsmFI GGGAC 1 cut(s) 901
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 5 cut(s) 6, 38, 632, 1064, 1152
BsoBI CYCGRG 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 18, 293
Bsp13I TCCGGA 1 cut(s) 800
Bsp143I GATC 5 cut(s) 95, 202, 511, 982, 1111
BspACI CCGC 2 cut(s) 768, 781
BspANI GGCC 5 cut(s) 6, 38, 632, 1064, 1152
BspCNI CTCAG 4 cut(s) 708, 857, 1111, 1183
BspEI TCCGGA 1 cut(s) 800
BspFNI CGCG 2 cut(s) 393, 807
BspLI GGNNCC 2 cut(s) 453, 766
BspPI GGATC 1 cut(s) 977
BspT107I GGYRCC 2 cut(s) 451, 764
BspTI CTTAAG 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 1106
BsrFI RCCGGY 2 cut(s) 6, 816
BsrI ACTGG 4 cut(s) 244, 730, 889, 1087
BssAI RCCGGY 2 cut(s) 6, 816
BssECI CCNNGG 1 cut(s) 286
BssHII GCGCGC 1 cut(s) 391
BssMI GATC 5 cut(s) 95, 202, 511, 982, 1111
BssNAI GTATAC 1 cut(s) 55
BssNI GRCGYC 1 cut(s) 765
Bst1107I GTATAC 1 cut(s) 55
Bst2UI CCWGG 4 cut(s) 50, 287, 634, 1058
Bst4CI ACNGT 3 cut(s) 118, 812, 862
Bst6I CTCTTC 2 cut(s) 693, 1125
BstACI GRCGYC 1 cut(s) 765
BstAFI CTTAAG 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 393, 818, 1017
BstDEI CTNAG 4 cut(s) 695, 844, 1119, 1170
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 3 cut(s) 216, 825, 1035
BstFNI CGCG 2 cut(s) 393, 807
BstH2I RGCGCY 2 cut(s) 768, 1057
BstHHI GCGC 4 cut(s) 393, 395, 767, 1056
BstKTI GATC 5 cut(s) 98, 205, 514, 985, 1114
BstMBI GATC 5 cut(s) 95, 202, 511, 982, 1111
BstMWI GCNNNNNNNGC 4 cut(s) 813, 822, 956, 1070
BstNI CCWGG 4 cut(s) 50, 287, 634, 1058
BstNSI RCATGY 3 cut(s) 175, 577, 658
BstSCI CCNGG 4 cut(s) 48, 285, 632, 1056
BstSLI GKGCMC 1 cut(s) 293
BstUI CGCG 2 cut(s) 393, 807
BstV1I GCAGC 2 cut(s) 421, 934
BstV2I GAAGAC 1 cut(s) 460
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BstZ17I GTATAC 1 cut(s) 55
BsuRI GGCC 5 cut(s) 6, 38, 632, 1064, 1152
BtsCI GGATG 3 cut(s) 216, 825, 1035
Cac8I GCNNGC 3 cut(s) 393, 818, 1017
CfoI GCGC 4 cut(s) 393, 395, 767, 1056
Cfr10I RCCGGY 2 cut(s) 6, 816
Cfr13I GGNCC 5 cut(s) 4, 9, 36, 46, 1151
CpoI CGGWCCG 1 cut(s) 9
CseI GACGC 2 cut(s) 254, 813
CsiI ACCWGGT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 119, 452
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CspI CGGWCCG 1 cut(s) 9
CviAII CATG 7 cut(s) 43, 172, 422, 499, 574, 655, 964
CviQI GTAC 2 cut(s) 119, 452
DdeI CTNAG 4 cut(s) 695, 844, 1119, 1170
DinI GGCGCC 1 cut(s) 766
DpnI GATC 5 cut(s) 97, 204, 513, 984, 1113
DpnII GATC 5 cut(s) 95, 202, 511, 982, 1111
DrdI GACNNNNNNGTC 1 cut(s) 810
DseDI GACNNNNNNGTC 1 cut(s) 810
Eam1104I CTCTTC 2 cut(s) 693, 1125
EarI CTCTTC 2 cut(s) 693, 1125
Eco147I AGGCCT 2 cut(s) 632, 1064
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 9, 46
Eco57I CTGAAG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 17
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoO109I RGGNCCY 1 cut(s) 1151
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 4 cut(s) 48, 285, 632, 1056
EcoT38I GRGCYC 1 cut(s) 18
EgeI GGCGCC 1 cut(s) 766
EheI GGCGCC 1 cut(s) 766
FaeI CATG 7 cut(s) 46, 175, 425, 502, 577, 658, 967
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 901
FatI CATG 7 cut(s) 42, 171, 421, 498, 573, 654, 963
FauNDI CATATG 2 cut(s) 354, 387
FbaI TGATCA 1 cut(s) 1111
FblI GTMKAC 2 cut(s) 54, 518
Fnu4HI GCNGC 3 cut(s) 435, 768, 948
FokI GGATG 3 cut(s) 223, 812, 1042
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 435, 768, 948
FspBI CTAG 3 cut(s) 149, 1070, 1219
GlaI GCGC 4 cut(s) 392, 394, 766, 1055
GluI GCNGC 3 cut(s) 435, 768, 948
GsuI CTGGAG 1 cut(s) 1079
HaeII RGCGCY 2 cut(s) 768, 1057
HaeIII GGCC 5 cut(s) 6, 38, 632, 1064, 1152
HapII CCGG 4 cut(s) 7, 791, 801, 817
HgaI GACGC 2 cut(s) 254, 813
HhaI GCGC 4 cut(s) 393, 395, 767, 1056
Hin1I GRCGYC 1 cut(s) 765
Hin1II CATG 7 cut(s) 46, 175, 425, 502, 577, 658, 967
Hin6I GCGC 4 cut(s) 391, 393, 765, 1054
HinP1I GCGC 4 cut(s) 391, 393, 765, 1054
HincII GTYRAC 3 cut(s) 445, 579, 754
HindII GTYRAC 3 cut(s) 445, 579, 754
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 5 cut(s) 20, 868, 1022, 1036, 1076
HpaII CCGG 4 cut(s) 7, 791, 801, 817
HphI GGTGA 2 cut(s) 726, 860
Hpy166II GTNNAC 6 cut(s) 55, 445, 519, 579, 754, 896
Hpy188I TCNGA 5 cut(s) 13, 25, 130, 873, 1173
Hpy188III TCNNGA 6 cut(s) 408, 801, 829, 880, 1040, 1204
Hpy8I GTNNAC 6 cut(s) 55, 445, 519, 579, 754, 896
HpyAV CCTTC 5 cut(s) 599, 755, 961, 1138, 1142
HpyCH4III ACNGT 3 cut(s) 118, 812, 862
HpyCH4V TGCA 3 cut(s) 476, 950, 1184
HpyF10VI GCNNNNNNNGC 4 cut(s) 813, 822, 956, 1070
HpyF3I CTNAG 4 cut(s) 695, 844, 1119, 1170
Hsp92I GRCGYC 1 cut(s) 765
Hsp92II CATG 7 cut(s) 46, 175, 425, 502, 577, 658, 967
HspAI GCGC 4 cut(s) 391, 393, 765, 1054
KasI GGCGCC 1 cut(s) 764
Kpn2I TCCGGA 1 cut(s) 800
KpnI GGTACC 1 cut(s) 455
KroI GCCGGC 1 cut(s) 816
KroNI GCCGGC 1 cut(s) 818
Ksp22I TGATCA 1 cut(s) 1111
Kzo9I GATC 5 cut(s) 95, 202, 511, 982, 1111
LmnI GCTCC 1 cut(s) 977
Lsp1109I GCAGC 2 cut(s) 421, 934
LweI GCATC 3 cut(s) 49, 388, 834
MabI ACCWGGT 1 cut(s) 48
MaeI CTAG 3 cut(s) 149, 1070, 1219
MalI GATC 5 cut(s) 97, 204, 513, 984, 1113
MboI GATC 5 cut(s) 95, 202, 511, 982, 1111
MboII GAAGA 6 cut(s) 460, 614, 680, 1139, 1142, 1175
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 18, 293
MluCI AATT 7 cut(s) 258, 281, 306, 560, 585, 593, 1158
Mly113I GGCGCC 1 cut(s) 765
MlyI GAGTC 2 cut(s) 29, 1070
MmeI TCCRAC 2 cut(s) 49, 1104
MroI TCCGGA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 816
MseI TTAA 5 cut(s) 185, 462, 504, 906, 1095
MslI CAYNNNNRTG 2 cut(s) 426, 921
MspCI CTTAAG 1 cut(s) 184
MspI CCGG 4 cut(s) 7, 791, 801, 817
MspR9I CCNGG 4 cut(s) 50, 287, 634, 1058
Mva1269I GAATGC 1 cut(s) 150
MvaI CCWGG 4 cut(s) 50, 287, 634, 1058
MvnI CGCG 2 cut(s) 393, 807
MwoI GCNNNNNNNGC 4 cut(s) 813, 822, 956, 1070
NaeI GCCGGC 1 cut(s) 818
NarI GGCGCC 1 cut(s) 765
NdeI CATATG 2 cut(s) 354, 387
NdeII GATC 5 cut(s) 95, 202, 511, 982, 1111
NgoMIV GCCGGC 1 cut(s) 816
NlaIII CATG 7 cut(s) 46, 175, 425, 502, 577, 658, 967
NlaIV GGNNCC 2 cut(s) 453, 766
NspI RCATGY 3 cut(s) 175, 577, 658
PauI GCGCGC 1 cut(s) 391
PceI AGGCCT 2 cut(s) 632, 1064
PciI ACATGT 3 cut(s) 171, 573, 654
PctI GAATGC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 818
PfeI GAWTC 3 cut(s) 868, 1022, 1036
PkrI GCNGC 3 cut(s) 436, 769, 949
PleI GAGTC 2 cut(s) 28, 1070
PluTI GGCGCC 1 cut(s) 768
PpsI GAGTC 2 cut(s) 28, 1070
PscI ACATGT 3 cut(s) 171, 573, 654
Psp6I CCWGG 4 cut(s) 48, 285, 632, 1056
PspGI CCWGG 4 cut(s) 48, 285, 632, 1056
PspN4I GGNNCC 2 cut(s) 453, 766
PspPI GGNCC 5 cut(s) 4, 9, 36, 46, 1151
PsuI RGATCY 1 cut(s) 202
PteI GCGCGC 1 cut(s) 391
RsaI GTAC 2 cut(s) 120, 453
RsaNI GTAC 2 cut(s) 119, 452
RseI CAYNNNNRTG 2 cut(s) 426, 921
Rsr2I CGGWCCG 1 cut(s) 9
RsrII CGGWCCG 1 cut(s) 9
SaqAI TTAA 5 cut(s) 185, 462, 504, 906, 1095
SatI GCNGC 3 cut(s) 435, 768, 948
Sau3AI GATC 5 cut(s) 95, 202, 511, 982, 1111
Sau96I GGNCC 5 cut(s) 4, 9, 36, 46, 1151
SchI GAGTC 2 cut(s) 29, 1070
ScrFI CCNGG 4 cut(s) 50, 287, 634, 1058
SduI GDGCHC 2 cut(s) 18, 293
SexAI ACCWGGT 1 cut(s) 48
SfaNI GCATC 3 cut(s) 49, 388, 834
SfoI GGCGCC 1 cut(s) 766
SinI GGWCC 2 cut(s) 9, 46
SmiMI CAYNNNNRTG 2 cut(s) 426, 921
SmlI CTYRAG 2 cut(s) 184, 995
SmoI CTYRAG 2 cut(s) 184, 995
Sse9I AATT 7 cut(s) 258, 281, 306, 560, 585, 593, 1158
SseBI AGGCCT 2 cut(s) 632, 1064
SsiI CCGC 2 cut(s) 768, 781
SspDI GGCGCC 1 cut(s) 764
SspI AATATT 1 cut(s) 318
SspMI CTAG 3 cut(s) 149, 1070, 1219
StuI AGGCCT 2 cut(s) 632, 1064
StyD4I CCNGG 4 cut(s) 48, 285, 632, 1056
TaaI ACNGT 3 cut(s) 118, 812, 862
TaqI TCGA 1 cut(s) 1048
TasI AATT 7 cut(s) 258, 281, 306, 560, 585, 593, 1158
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 3 cut(s) 868, 1022, 1036
Tru1I TTAA 5 cut(s) 185, 462, 504, 906, 1095
Tru9I TTAA 5 cut(s) 185, 462, 504, 906, 1095
TseI GCWGC 2 cut(s) 434, 947
TspDTI ATGAA 5 cut(s) 174, 629, 680, 905, 980
Vha464I CTTAAG 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 9, 46
XagI CCTNNNNNAGG 1 cut(s) 905
XapI RAATTY 1 cut(s) 281
XceI RCATGY 3 cut(s) 175, 577, 658
XmiI GTMKAC 2 cut(s) 54, 518
XspI CTAG 3 cut(s) 149, 1070, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.