Rmu_sc0001403.1_g000009

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001403.1
N/A
Physical Location & Seq
Reverse (-)
22212 .. 26367
4156 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1230 bp
atgggcggagagaggaaggttgtgtgtgttacaggagcttctggtttcatagcttcatggctggtgaagattttgttacagcgaggttatgtcgtcaaagccaccgttcgtgacccaaatgatccaaagaaaacagaacacttgctctcacttgatggagcaaaggaaaggcttcatttgttcaaagcagaattgttagaagaggggtgttttgaccttgtagttgatggatgtgaaggtgttttccatacagcatctccggtactattttcatcgatcagtgacccacaggcagaactgattgatcctgcagtaaagggaacgcttaatgttcttaaatcatgcgtgaaatttccaacaatcaagagggtagttttaacatcttccatggctgcagttattatcaatggaagacctttaacctctaatgtagaagttgatgaaacatggttttctgatccacttgtttgtgaggagttgaaggcatggtatttactttcaaaaactttagcagaggaggctgcttggaaatttgctaaagaaaatgggattgacatggtgaccataaatccagcgtatgtgatgggtccactcttgcagccaactcttaattacactgtggagatggttctgaatctcaagaatgatgctcacgaagtaacaaatggatattatttatccactgatgttagagatgttgcctcggctcatattcaagcatttgaagttccttcagcgagtggaagatattgtctagttgcaaatgtcacccccatttacaaggctctgaagattttagaacaacttcatccgactttgtgcccacctgaaatatgtgagcatgatatcccttctggcccagagtatcaaatatcccaggagaaggcaaaaagtttgggagttagtttccttccgttggaaaataagaagattcgaagaagtcaagaacaagaacttacatggagaacccgtgggctggaggctgaaggagaactgaaggagcggtggagtggaggggcgcggtggagcagagggagaccgaggcagagtcgcgcagatagagagagttgtggtggcacagtgaagaggaggagcgctgcgcaaggaggcccgacgccggaggctatgagggtcaccatggtgacccagaacactgtacaaggtgatccagggcaggaaatacactgtgctcgtaacccagagggtgaaattaatactaaaaagcagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

409

Amino Acids

45.21

Weight (kDa)

6.49

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 105 1.2e-06 NmrA-like family
Epimerase PF01370 8 - 246 6.5e-19 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 241 5.8e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 201 1.2e-10 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 129 1.1e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 132 1.3e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1101
AccBSI CCGCTC 1 cut(s) 1003
AccII CGCG 2 cut(s) 1021, 1053
AciI CCGC 3 cut(s) 6, 1003, 1021
AclWI GGATC 4 cut(s) 116, 299, 452, 1160
AcsI RAATTY 2 cut(s) 350, 530
AcuI CTGAAG 4 cut(s) 717, 809, 1005, 1016
AcyI GRCGYC 1 cut(s) 1115
AfaI GTAC 2 cut(s) 264, 1158
AfeI AGCGCT 1 cut(s) 1096
AfiI CCNNNNNNNGG 4 cut(s) 883, 916, 976, 1117
AgsI TTSAA 5 cut(s) 184, 481, 501, 716, 725
AjnI CCWGG 2 cut(s) 876, 1168
AleI CACNNNNGTG 1 cut(s) 1139
AluBI AGCT 2 cut(s) 38, 53
AluI AGCT 2 cut(s) 38, 53
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 1 cut(s) 1030
AlwI GGATC 4 cut(s) 116, 299, 452, 1160
Aor51HI AGCGCT 1 cut(s) 1096
AoxI GGCC 2 cut(s) 856, 1108
ApeKI GCWGC 4 cut(s) 392, 521, 598, 1097
ApoI RAATTY 2 cut(s) 350, 530
AseI ATTAAT 1 cut(s) 1212
Asp700I GAANNNNTTC 2 cut(s) 171, 804
AspLEI GCGC 4 cut(s) 1021, 1055, 1097, 1102
AspS9I GGNCC 3 cut(s) 587, 857, 1109
AsuHPI GGTGA 7 cut(s) 76, 571, 760, 1126, 1153, 1175, 1217
AsuII TTCGAA 1 cut(s) 934
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 824
BbsI GAAGAC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 4 cut(s) 379, 508, 610, 1084
BccI CCATC 4 cut(s) 149, 221, 577, 619
BciT130I CCWGG 2 cut(s) 878, 1170
BcoDI GTCTC 1 cut(s) 1030
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 2 cut(s) 309, 393
BfoI RGCGCY 1 cut(s) 1098
BisI GCNGC 4 cut(s) 393, 522, 599, 1098
BlsI GCNGC 4 cut(s) 394, 523, 600, 1099
Bme1390I CCNGG 2 cut(s) 878, 1170
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 3 cut(s) 587, 857, 1109
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 2 cut(s) 878, 1170
BmsI GCATC 2 cut(s) 263, 637
BpiI GAAGAC 1 cut(s) 418
BpmI CTGGAG 1 cut(s) 998
Bpu14I TTCGAA 1 cut(s) 934
BpuEI CTTGAG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 1115
BsaI GGTCTC 1 cut(s) 1030
BsaJI CCNNGG 7 cut(s) 387, 702, 876, 970, 1040, 1137, 1169
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 4 cut(s) 241, 271, 955, 985
Bsc4I CCNNNNNNNGG 4 cut(s) 883, 916, 976, 1117
BseBI CCWGG 2 cut(s) 878, 1170
BseCI ATCGAT 1 cut(s) 275
BseDI CCNNGG 7 cut(s) 387, 702, 876, 970, 1040, 1137, 1169
BseGI GGATG 2 cut(s) 236, 808
BseLI CCNNNNNNNGG 4 cut(s) 883, 916, 976, 1117
BseRI GAGGAG 4 cut(s) 488, 530, 1102, 1105
BseSI GKGCMC 1 cut(s) 824
BseXI GCAGC 4 cut(s) 379, 508, 610, 1084
Bsh1236I CGCG 2 cut(s) 1021, 1053
BshFI GGCC 2 cut(s) 858, 1110
BshVI ATCGAT 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 1192
BsiSI CCGG 2 cut(s) 260, 1118
BslI CCNNNNNNNGG 4 cut(s) 883, 916, 976, 1117
BsmAI GTCTC 1 cut(s) 1030
BsnI GGCC 2 cut(s) 858, 1110
Bso31I GGTCTC 1 cut(s) 1030
Bsp119I TTCGAA 1 cut(s) 934
Bsp1286I GDGCHC 2 cut(s) 824, 1192
Bsp1407I TGTACA 1 cut(s) 1156
Bsp143I GATC 5 cut(s) 121, 276, 304, 457, 1165
Bsp19I CCATGG 2 cut(s) 387, 1137
BspACI CCGC 3 cut(s) 6, 1003, 1021
BspANI GGCC 2 cut(s) 858, 1110
BspDI ATCGAT 1 cut(s) 275
BspFNI CGCG 2 cut(s) 1021, 1053
BspLI GGNNCC 1 cut(s) 588
BspMAI CTGCAG 2 cut(s) 313, 397
BspPI GGATC 4 cut(s) 116, 299, 452, 1160
BspT104I TTCGAA 1 cut(s) 934
BspTNI GGTCTC 1 cut(s) 1030
BsrBI CCGCTC 1 cut(s) 1003
BsrGI TGTACA 1 cut(s) 1156
BssECI CCNNGG 7 cut(s) 387, 702, 876, 970, 1040, 1137, 1169
BssMI GATC 5 cut(s) 121, 276, 304, 457, 1165
BssNI GRCGYC 1 cut(s) 1115
BssT1I CCWWGG 2 cut(s) 387, 1137
Bst2UI CCWGG 2 cut(s) 878, 1170
Bst4CI ACNGT 5 cut(s) 106, 619, 1081, 1156, 1187
Bst6I CTCTTC 2 cut(s) 195, 1079
BstACI GRCGYC 1 cut(s) 1115
BstAUI TGTACA 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 934
BstDSI CCRYGG 3 cut(s) 387, 970, 1137
BstEII GGTNACC 3 cut(s) 559, 1132, 1141
BstF5I GGATG 2 cut(s) 236, 808
BstFNI CGCG 2 cut(s) 1021, 1053
BstH2I RGCGCY 1 cut(s) 1098
BstHHI GCGC 4 cut(s) 1021, 1055, 1097, 1102
BstKTI GATC 5 cut(s) 124, 279, 307, 460, 1168
BstMAI GTCTC 1 cut(s) 1030
BstMBI GATC 5 cut(s) 121, 276, 304, 457, 1165
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstNI CCWGG 2 cut(s) 878, 1170
BstPI GGTNACC 3 cut(s) 559, 1132, 1141
BstSCI CCNGG 2 cut(s) 876, 1168
BstSFI CTRYAG 2 cut(s) 309, 393
BstSLI GKGCMC 1 cut(s) 824
BstUI CGCG 2 cut(s) 1021, 1053
BstV1I GCAGC 4 cut(s) 379, 508, 610, 1084
BstV2I GAAGAC 1 cut(s) 418
Bsu15I ATCGAT 1 cut(s) 275
BsuRI GGCC 2 cut(s) 858, 1110
BsuTUI ATCGAT 1 cut(s) 275
BtgI CCRYGG 3 cut(s) 387, 970, 1137
BtsCI GGATG 2 cut(s) 236, 808
BtsIMutI CAGTG 6 cut(s) 286, 615, 681, 1086, 1152, 1183
CfoI GCGC 4 cut(s) 1021, 1055, 1097, 1102
Cfr13I GGNCC 3 cut(s) 587, 857, 1109
ClaI ATCGAT 1 cut(s) 275
CseI GACGC 1 cut(s) 1123
Csp6I GTAC 2 cut(s) 263, 1157
CviAII CATG 9 cut(s) 57, 342, 388, 447, 486, 556, 842, 960, 1138
CviQI GTAC 2 cut(s) 263, 1157
DpnI GATC 5 cut(s) 123, 278, 306, 459, 1167
DpnII GATC 5 cut(s) 121, 276, 304, 457, 1165
Eam1104I CTCTTC 2 cut(s) 195, 1079
EarI CTCTTC 2 cut(s) 195, 1079
EciI GGCGGA 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 387, 1137
Eco31I GGTCTC 1 cut(s) 1030
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 1 cut(s) 587
Eco47III AGCGCT 1 cut(s) 1096
Eco57I CTGAAG 4 cut(s) 717, 809, 1005, 1016
Eco91I GGTNACC 3 cut(s) 559, 1132, 1141
EcoO65I GGTNACC 3 cut(s) 559, 1132, 1141
EcoRII CCWGG 2 cut(s) 876, 1168
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 387, 1137
ErhI CCWWGG 2 cut(s) 387, 1137
FaeI CATG 9 cut(s) 60, 345, 391, 450, 489, 559, 845, 963, 1141
FatI CATG 9 cut(s) 56, 341, 387, 446, 485, 555, 841, 959, 1137
Fnu4HI GCNGC 4 cut(s) 393, 522, 599, 1098
FokI GGATG 2 cut(s) 243, 795
Fsp4HI GCNGC 4 cut(s) 393, 522, 599, 1098
FspBI CTAG 1 cut(s) 755
FspI TGCGCA 1 cut(s) 1101
GlaI GCGC 4 cut(s) 1020, 1054, 1096, 1101
GluI GCNGC 4 cut(s) 393, 522, 599, 1098
GsuI CTGGAG 1 cut(s) 998
HaeII RGCGCY 1 cut(s) 1098
HaeIII GGCC 2 cut(s) 858, 1110
HapII CCGG 2 cut(s) 260, 1118
HgaI GACGC 1 cut(s) 1123
HhaI GCGC 4 cut(s) 1021, 1055, 1097, 1102
Hin1I GRCGYC 1 cut(s) 1115
Hin1II CATG 9 cut(s) 60, 345, 391, 450, 489, 559, 845, 963, 1141
Hin6I GCGC 4 cut(s) 1019, 1053, 1095, 1100
HinP1I GCGC 4 cut(s) 1019, 1053, 1095, 1100
HinfI GANTC 3 cut(s) 634, 931, 1048
HpaII CCGG 2 cut(s) 260, 1118
HphI GGTGA 7 cut(s) 76, 571, 760, 1126, 1153, 1175, 1217
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 4 cut(s) 457, 633, 789, 813
Hpy188III TCNNGA 5 cut(s) 110, 364, 640, 653, 944
Hpy8I GTNNAC 1 cut(s) 590
Hpy99I CGWCG 1 cut(s) 1117
HpyAV CCTTC 9 cut(s) 10, 230, 475, 741, 861, 877, 920, 980, 991
HpyCH4III ACNGT 5 cut(s) 106, 619, 1081, 1156, 1187
HpyCH4V TGCA 4 cut(s) 311, 395, 598, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
Hsp92I GRCGYC 1 cut(s) 1115
Hsp92II CATG 9 cut(s) 60, 345, 391, 450, 489, 559, 845, 963, 1141
HspAI GCGC 4 cut(s) 1019, 1053, 1095, 1100
Kzo9I GATC 5 cut(s) 121, 276, 304, 457, 1165
LmnI GCTCC 5 cut(s) 35, 158, 1000, 1026, 1092
Lsp1109I GCAGC 4 cut(s) 379, 508, 610, 1084
LweI GCATC 2 cut(s) 263, 637
MaeI CTAG 1 cut(s) 755
MalI GATC 5 cut(s) 123, 278, 306, 459, 1167
MbiI CCGCTC 1 cut(s) 1003
MboI GATC 5 cut(s) 121, 276, 304, 457, 1165
MboII GAAGA 9 cut(s) 79, 212, 375, 423, 756, 802, 940, 948, 1096
MhlI GDGCHC 2 cut(s) 824, 1192
MluCI AATT 5 cut(s) 191, 350, 530, 610, 1209
MlyI GAGTC 1 cut(s) 1057
MmeI TCCRAC 3 cut(s) 380, 836, 897
MroXI GAANNNNTTC 2 cut(s) 171, 804
MseI TTAA 6 cut(s) 327, 336, 377, 419, 609, 1212
MslI CAYNNNNRTG 1 cut(s) 1139
MspI CCGG 2 cut(s) 260, 1118
MspR9I CCNGG 2 cut(s) 878, 1170
MteI GCGCNGCGC 1 cut(s) 1098
MvaI CCWGG 2 cut(s) 878, 1170
MvnI CGCG 2 cut(s) 1021, 1053
MwoI GCNNNNNNNGC 1 cut(s) 518
NcoI CCATGG 2 cut(s) 387, 1137
NdeII GATC 5 cut(s) 121, 276, 304, 457, 1165
NlaIII CATG 9 cut(s) 60, 345, 391, 450, 489, 559, 845, 963, 1141
NlaIV GGNNCC 1 cut(s) 588
NmeAIII GCCGAG 1 cut(s) 683
NmuCI GTSAC 6 cut(s) 110, 281, 559, 766, 1132, 1141
NsbI TGCGCA 1 cut(s) 1101
NspV TTCGAA 1 cut(s) 934
OliI CACNNNNGTG 1 cut(s) 1139
PdmI GAANNNNTTC 2 cut(s) 171, 804
PfeI GAWTC 2 cut(s) 634, 931
PkrI GCNGC 4 cut(s) 394, 523, 600, 1099
PleI GAGTC 1 cut(s) 1056
PpsI GAGTC 1 cut(s) 1056
PshBI ATTAAT 1 cut(s) 1212
Psp6I CCWGG 2 cut(s) 876, 1168
PspEI GGTNACC 3 cut(s) 559, 1132, 1141
PspGI CCWGG 2 cut(s) 876, 1168
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 3 cut(s) 587, 857, 1109
PstI CTGCAG 2 cut(s) 313, 397
RsaI GTAC 2 cut(s) 264, 1158
RsaNI GTAC 2 cut(s) 263, 1157
RseI CAYNNNNRTG 1 cut(s) 1139
SaqAI TTAA 6 cut(s) 327, 336, 377, 419, 609, 1212
SatI GCNGC 4 cut(s) 393, 522, 599, 1098
Sau3AI GATC 5 cut(s) 121, 276, 304, 457, 1165
Sau96I GGNCC 3 cut(s) 587, 857, 1109
SchI GAGTC 1 cut(s) 1057
ScrFI CCNGG 2 cut(s) 878, 1170
SduI GDGCHC 2 cut(s) 824, 1192
SfaNI GCATC 2 cut(s) 263, 637
SfcI CTRYAG 2 cut(s) 309, 393
SfuI TTCGAA 1 cut(s) 934
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 5 cut(s) 191, 350, 530, 610, 1209
SsiI CCGC 3 cut(s) 6, 1003, 1021
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 2 cut(s) 876, 1168
StyI CCWWGG 2 cut(s) 387, 1137
TaaI ACNGT 5 cut(s) 106, 619, 1081, 1156, 1187
TaqI TCGA 2 cut(s) 275, 934
TaqII GACCGA 1 cut(s) 1054
TasI AATT 5 cut(s) 191, 350, 530, 610, 1209
TatI WGTACW 1 cut(s) 1156
TfiI GAWTC 2 cut(s) 634, 931
Tru1I TTAA 6 cut(s) 327, 336, 377, 419, 609, 1212
Tru9I TTAA 6 cut(s) 327, 336, 377, 419, 609, 1212
TscAI CASTG 6 cut(s) 286, 622, 688, 1086, 1159, 1190
TseFI GTSAC 6 cut(s) 110, 281, 559, 766, 1132, 1141
TseI GCWGC 4 cut(s) 392, 521, 598, 1097
Tsp45I GTSAC 6 cut(s) 110, 281, 559, 766, 1132, 1141
TspDTI ATGAA 6 cut(s) 37, 45, 164, 261, 456, 797
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 6 cut(s) 286, 622, 688, 1086, 1159, 1190
VpaK11BI GGWCC 1 cut(s) 587
VspI ATTAAT 1 cut(s) 1212
XapI RAATTY 2 cut(s) 350, 530
XmnI GAANNNNTTC 2 cut(s) 171, 804
XspI CTAG 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.