Rmu_sc0001448.1_g000010

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001448.1
N/A
Physical Location & Seq
Reverse (-)
64843 .. 71004
6162 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
atgttcatgtttctccgattagagtcatcaaacttcaagatgtgttatgtttataaaattaccattaattctcaaactttccagatcagaagtagaggtcctaagcttcaaattgatataaaattctgcggtgctgcacagaacctgcaactgcaggtgaggtggctgcccagaaaccggcaagcccgacctcctccgacctcgaacgccgcccagaagaggtctgggactcctccggcctccctccggccagctccgcgccgccgtcgtcgcctggaaccctcctgctgcatcgacgtccgcactttagcgaaaaggctcgacgcagccatctcgtcccgccaagggcagatccgcctccgccgccttcacgccgtgcacgtgagatcggatcctccaaccattcttcatccctactactcaggcaagactcgccaccccacggcagatccaagagcaaccgcccgaacccgatcgaagcagactgaccggatcgaaaacccatggaacccagcgaagccgccacccggttttcaccgtcacgagacatccgatcccgccacgccaactctgaacgaccaccacaccgtccgcactccgcccaaggatagagcgaccccgatcccacttgcctaccgccgacggcgaagccttggagcgatctggtttgtgccgtcatgcccccccgcatcttcttctccgactggacctctcacatccggacaccaggtccattctgtcccttccgacgacgacgacgacgacgccgcgaaggcgcgccgccggacagaatacggcggcggtacgacggttatgggtttcgtgattgtgttgggggttatcgtttgtgtgtcagcattctgcaagtattgtatggcggcggagaagagaaacggtaactgtgccggggttgggaaactaagcgtggtgaagcttcaggttggtttgatgggcaaggcacagtcaatccaaagggatcttaatcaaattactgaaactacaaatacttctactccaaagggtctgagctatgttttgacaggttacaattttggtggctactcaaggagcatcaatggccctggtgacttgaagaaagcattacagagtcttggatccattccctcgaacaaaatactggcggttgaggtcttagggacactcctcaacaagaaaacgatactttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

395

Amino Acids

43.63

Weight (kDa)

10.55

Isoelectric Point (pI)

62.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 307 - 347 1.2e-08 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
AarI CACCTGC 1 cut(s) 145
AatII GACGTC 1 cut(s) 300
Acc36I ACCTGC 2 cut(s) 145, 153
AccII CGCG 3 cut(s) 259, 770, 778
AccIII TCCGGA 1 cut(s) 719
AcoI YGGCCR 1 cut(s) 248
AcsI RAATTY 1 cut(s) 122
AcuI CTGAAG 1 cut(s) 920
AcvI CACGTG 1 cut(s) 382
AcyI GRCGYC 2 cut(s) 297, 765
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 7 cut(s) 177, 219, 246, 345, 442, 527, 912
AgsI TTSAA 3 cut(s) 37, 110, 1093
AhdI GACNNNNNGTC 1 cut(s) 728
AjnI CCWGG 3 cut(s) 273, 726, 1081
AluBI AGCT 4 cut(s) 106, 254, 934, 1029
AluI AGCT 4 cut(s) 106, 254, 934, 1029
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 539
Alw44I GTGCAC 1 cut(s) 377
AlwNI CAGNNNCTG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 719
AoxI GGCC 3 cut(s) 237, 248, 1078
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 4 cut(s) 134, 166, 288, 326
ApoI RAATTY 1 cut(s) 122
AscI GGCGCGCC 1 cut(s) 776
AseI ATTAAT 1 cut(s) 66
AspLEI GCGC 3 cut(s) 261, 778, 780
AspS9I GGNCC 4 cut(s) 98, 707, 730, 1079
AsuC2I CCSGG 2 cut(s) 528, 907
AsuHPI GGTGA 4 cut(s) 169, 527, 940, 1097
AvaII GGWCC 3 cut(s) 98, 707, 730
BaeGI GKGCMC 1 cut(s) 381
BaeI ACNNNNGTAYC 2 cut(s) 795, 828
BamHI GGATCC 2 cut(s) 391, 1115
BauI CACGAG 1 cut(s) 542
BbrPI CACGTG 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 4 cut(s) 121, 153, 275, 338
BccI CCATC 2 cut(s) 338, 943
BceAI ACGGC 6 cut(s) 249, 359, 459, 658, 659, 811
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BciT130I CCWGG 3 cut(s) 275, 728, 1083
BcnI CCSGG 2 cut(s) 528, 907
BcoDI GTCTC 1 cut(s) 539
BfmI CTRYAG 1 cut(s) 152
BfuAI ACCTGC 2 cut(s) 145, 153
BglI GCCNNNNNGGC 1 cut(s) 773
Bme1390I CCNGG 5 cut(s) 275, 528, 728, 907, 1083
Bme18I GGWCC 3 cut(s) 98, 707, 730
BmeRI GACNNNNNGTC 1 cut(s) 728
BmgT120I GGNCC 4 cut(s) 98, 707, 730, 1079
BmiI GGNNCC 4 cut(s) 279, 393, 509, 1117
BmrFI CCNGG 5 cut(s) 275, 528, 728, 907, 1083
BmsI GCATC 3 cut(s) 300, 698, 1080
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 1048
BpuMI CCSGG 2 cut(s) 528, 907
BsaAI YACGTR 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 981
BsaHI GRCGYC 2 cut(s) 297, 765
BsaJI CCNNGG 7 cut(s) 343, 441, 503, 603, 652, 906, 1081
BsaWI WCCGGW 2 cut(s) 489, 719
BsaXI ACNNNNNCTCC 2 cut(s) 997, 1027
Bsc4I CCNNNNNNNGG 7 cut(s) 177, 219, 246, 345, 442, 527, 912
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 2 cut(s) 709, 1143
Bse8I GATNNNNATC 1 cut(s) 981
BseAI TCCGGA 1 cut(s) 719
BseBI CCWGG 3 cut(s) 275, 728, 1083
BseDI CCNNGG 7 cut(s) 343, 441, 503, 603, 652, 906, 1081
BseGI GGATG 3 cut(s) 409, 548, 716
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 7 cut(s) 177, 219, 246, 345, 442, 527, 912
BseMII CTCAG 2 cut(s) 435, 1016
BseNI ACTGG 2 cut(s) 709, 1143
BsePI GCGCGC 1 cut(s) 776
BseRI GAGGAG 3 cut(s) 183, 222, 1154
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 4 cut(s) 121, 153, 275, 338
BseYI CCCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 3 cut(s) 259, 770, 778
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 3 cut(s) 239, 250, 1080
BsiEI CGRYCG 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 381
BsiSI CCGG 8 cut(s) 178, 236, 247, 490, 528, 720, 784, 906
BslFI GGGAC 4 cut(s) 241, 322, 725, 1171
BslI CCNNNNNNNGG 7 cut(s) 177, 219, 246, 345, 442, 527, 912
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 4 cut(s) 241, 322, 725, 1171
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 3 cut(s) 239, 250, 1080
Bsp1286I GDGCHC 1 cut(s) 381
Bsp13I TCCGGA 1 cut(s) 719
Bsp19I CCATGG 1 cut(s) 503
BspANI GGCC 3 cut(s) 239, 250, 1080
BspCNI CTCAG 2 cut(s) 434, 1017
BspEI TCCGGA 1 cut(s) 719
BspFNI CGCG 3 cut(s) 259, 770, 778
BspLI GGNNCC 4 cut(s) 279, 393, 509, 1117
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 2 cut(s) 145, 153
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 2 cut(s) 709, 1143
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 7 cut(s) 343, 441, 503, 603, 652, 906, 1081
BssHII GCGCGC 1 cut(s) 776
BssNI GRCGYC 2 cut(s) 297, 765
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 4 cut(s) 343, 503, 603, 652
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 3 cut(s) 275, 728, 1083
Bst4CI ACNGT 6 cut(s) 539, 589, 811, 896, 902, 963
Bst6I CTCTTC 2 cut(s) 212, 881
BstACI GRCGYC 2 cut(s) 297, 765
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 3 cut(s) 183, 252, 778
BstDEI CTNAG 5 cut(s) 102, 421, 920, 1025, 1153
BstDSI CCRYGG 2 cut(s) 441, 503
BstF5I GGATG 3 cut(s) 409, 548, 716
BstFNI CGCG 3 cut(s) 259, 770, 778
BstHHI GCGC 3 cut(s) 261, 778, 780
BstMAI GTCTC 1 cut(s) 539
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 6 cut(s) 256, 270, 363, 432, 773, 1077
BstNI CCWGG 3 cut(s) 275, 728, 1083
BstSCI CCNGG 5 cut(s) 273, 526, 726, 905, 1081
BstSFI CTRYAG 1 cut(s) 152
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 3 cut(s) 259, 770, 778
BstV1I GCAGC 4 cut(s) 121, 153, 275, 338
BstX2I RGATCY 5 cut(s) 351, 391, 448, 976, 1115
BstYI RGATCY 5 cut(s) 351, 391, 448, 976, 1115
BsuRI GGCC 3 cut(s) 239, 250, 1080
BtgI CCRYGG 2 cut(s) 441, 503
BtsCI GGATG 3 cut(s) 409, 548, 716
BveI ACCTGC 2 cut(s) 145, 153
Cac8I GCNNGC 3 cut(s) 183, 252, 778
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 3 cut(s) 261, 778, 780
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 4 cut(s) 98, 707, 730, 1079
CseI GACGC 2 cut(s) 332, 773
CsiI ACCWGGT 1 cut(s) 726
Csp6I GTAC 1 cut(s) 804
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 3 cut(s) 7, 504, 678
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 5 cut(s) 102, 421, 920, 1025, 1153
DraIII CACNNNGTG 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 728
EaeI YGGCCR 1 cut(s) 248
Eam1104I CTCTTC 2 cut(s) 212, 881
Eam1105I GACNNNNNGTC 1 cut(s) 728
EarI CTCTTC 2 cut(s) 212, 881
EciI GGCGGA 4 cut(s) 344, 350, 588, 896
Eco130I CCWWGG 4 cut(s) 343, 503, 603, 652
Eco47I GGWCC 3 cut(s) 98, 707, 730
Eco57I CTGAAG 1 cut(s) 920
Eco72I CACGTG 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 273, 726, 1081
EcoT14I CCWWGG 4 cut(s) 343, 503, 603, 652
ErhI CCWWGG 4 cut(s) 343, 503, 603, 652
FaeI CATG 3 cut(s) 10, 507, 681
FaiI YATR 9 cut(s) 8, 48, 54, 119, 505, 679, 815, 875, 1032
FaqI GGGAC 4 cut(s) 241, 322, 725, 1171
FatI CATG 3 cut(s) 6, 503, 677
FauI CCCGC 3 cut(s) 347, 565, 694
FokI GGATG 3 cut(s) 396, 535, 703
GlaI GCGC 3 cut(s) 260, 777, 779
GsaI CCCAGC 1 cut(s) 515
HaeIII GGCC 3 cut(s) 239, 250, 1080
HapII CCGG 8 cut(s) 178, 236, 247, 490, 528, 720, 784, 906
HgaI GACGC 2 cut(s) 332, 773
HhaI GCGC 3 cut(s) 261, 778, 780
Hin1I GRCGYC 2 cut(s) 297, 765
Hin1II CATG 3 cut(s) 10, 507, 681
Hin6I GCGC 3 cut(s) 259, 776, 778
HinP1I GCGC 3 cut(s) 259, 776, 778
HindIII AAGCTT 2 cut(s) 104, 932
HinfI GANTC 4 cut(s) 23, 229, 430, 1108
HpaII CCGG 8 cut(s) 178, 236, 247, 490, 528, 720, 784, 906
HphI GGTGA 4 cut(s) 169, 527, 940, 1097
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 9 cut(s) 17, 89, 198, 391, 553, 573, 702, 748, 1026
Hpy188III TCNNGA 5 cut(s) 37, 82, 542, 720, 823
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 3 cut(s) 377, 753, 766
HpyCH4III ACNGT 6 cut(s) 539, 589, 811, 896, 902, 963
HpyCH4IV ACGT 2 cut(s) 297, 381
HpyCH4V TGCA 6 cut(s) 137, 148, 154, 291, 379, 864
HpyF10VI GCNNNNNNNGC 6 cut(s) 256, 270, 363, 432, 773, 1077
HpyF3I CTNAG 5 cut(s) 102, 421, 920, 1025, 1153
HpySE526I ACGT 2 cut(s) 297, 381
Hsp92I GRCGYC 2 cut(s) 297, 765
Hsp92II CATG 3 cut(s) 10, 507, 681
HspAI GCGC 3 cut(s) 259, 776, 778
Kpn2I TCCGGA 1 cut(s) 719
LmnI GCTCC 3 cut(s) 259, 656, 1068
Lsp1109I GCAGC 4 cut(s) 121, 153, 275, 338
LweI GCATC 3 cut(s) 300, 698, 1080
MabI ACCWGGT 1 cut(s) 726
MaeII ACGT 2 cut(s) 297, 381
MaeIII GTNAC 4 cut(s) 539, 896, 1043, 1085
MboII GAAGA 6 cut(s) 229, 398, 684, 687, 898, 1105
MflI RGATCY 5 cut(s) 351, 391, 448, 976, 1115
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 6 cut(s) 57, 67, 111, 122, 987, 1048
MlyI GAGTC 4 cut(s) 32, 223, 424, 1117
MmeI TCCRAC 4 cut(s) 221, 422, 725, 771
MroI TCCGGA 1 cut(s) 719
MseI TTAA 2 cut(s) 66, 981
MspI CCGG 8 cut(s) 178, 236, 247, 490, 528, 720, 784, 906
MspR9I CCNGG 5 cut(s) 275, 528, 728, 907, 1083
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 3 cut(s) 275, 728, 1083
MvnI CGCG 3 cut(s) 259, 770, 778
MwoI GCNNNNNNNGC 6 cut(s) 256, 270, 363, 432, 773, 1077
NciI CCSGG 2 cut(s) 528, 907
NcoI CCATGG 1 cut(s) 503
NlaIII CATG 3 cut(s) 10, 507, 681
NlaIV GGNNCC 4 cut(s) 279, 393, 509, 1117
NmuCI GTSAC 2 cut(s) 539, 1085
PalAI GGCGCGCC 1 cut(s) 776
PaqCI CACCTGC 1 cut(s) 145
PauI GCGCGC 1 cut(s) 776
PcsI WCGNNNNNNNCGW 4 cut(s) 378, 549, 756, 759
PctI GAATGC 1 cut(s) 857
Ple19I CGATCG 1 cut(s) 476
PleI GAGTC 4 cut(s) 31, 223, 424, 1116
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 4 cut(s) 31, 223, 424, 1116
Ppu21I YACGTR 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 66
PsiI TTATAA 1 cut(s) 54
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 273, 726, 1081
PspCI CACGTG 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 511
PspGI CCWGG 3 cut(s) 273, 726, 1081
PspN4I GGNNCC 4 cut(s) 279, 393, 509, 1117
PspPI GGNCC 4 cut(s) 98, 707, 730, 1079
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 5 cut(s) 351, 391, 448, 976, 1115
PteI GCGCGC 1 cut(s) 776
PvuI CGATCG 1 cut(s) 476
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
SaqAI TTAA 2 cut(s) 66, 981
Sau96I GGNCC 4 cut(s) 98, 707, 730, 1079
SchI GAGTC 4 cut(s) 32, 223, 424, 1117
ScrFI CCNGG 5 cut(s) 275, 528, 728, 907, 1083
SduI GDGCHC 1 cut(s) 381
SexAI ACCWGGT 1 cut(s) 726
SfaNI GCATC 3 cut(s) 300, 698, 1080
SfcI CTRYAG 1 cut(s) 152
SgsI GGCGCGCC 1 cut(s) 776
SinI GGWCC 3 cut(s) 98, 707, 730
SmlI CTYRAG 1 cut(s) 1063
SmoI CTYRAG 1 cut(s) 1063
Sse9I AATT 6 cut(s) 57, 67, 111, 122, 987, 1048
StyD4I CCNGG 5 cut(s) 273, 526, 726, 905, 1081
StyI CCWWGG 4 cut(s) 343, 503, 603, 652
TaaI ACNGT 6 cut(s) 539, 589, 811, 896, 902, 963
TaiI ACGT 2 cut(s) 300, 384
TaqI TCGA 6 cut(s) 203, 294, 321, 476, 495, 1127
TasI AATT 6 cut(s) 57, 67, 111, 122, 987, 1048
TauI GCSGC 8 cut(s) 212, 264, 366, 523, 770, 783, 801, 881
Tru1I TTAA 2 cut(s) 66, 981
Tru9I TTAA 2 cut(s) 66, 981
TseFI GTSAC 2 cut(s) 539, 1085
TseI GCWGC 4 cut(s) 134, 166, 288, 326
Tsp45I GTSAC 2 cut(s) 539, 1085
TspDTI ATGAA 1 cut(s) 398
VneI GTGCAC 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 98, 707, 730
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.