Rmu_sc0001533.1_g000003

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001533.1
N/A
Physical Location & Seq
Reverse (-)
8842 .. 9814
973 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
atgcatgagatggttccagtgaaacgttttgatcgttaccatgaattgggtcagcatgcatttggtgaaaagcttggtctttacatcgtggggcctcagcaactcattgttgaagttggtgtttgcattgtgtacatggtcaccggaggaaaatcgctgcagaaggtccatgacactgtgtgctcagactgcaaaaagatcaaattgacttacttcatcatgatctttgcctctgttcactttgtgctttcccaccttcccaacttcaactccatctccggcgtgtctttggccgcagctgtcatgtccttgagttacactaccatagcatggactgctgcggtagacaagggtgtggtagaaaatgtcgattgtgtcctcgaaatccaagctacaattccttctacacctgagaagccttccaaggtacctatgtggaaaggagtcattgttgcctacataattgtggctttgtgctacttcccagttgctctagtaggatattacatatatggaaattcaaatgatgacaacatcctcgtctcattggaaagacccaaatggctaattgtaatggccaacatgtttgttgtcgtccctgttattggaaactatcaggtgatgcacggatcgaggattgcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

214

Amino Acids

23.7

Weight (kDa)

7.66

Isoelectric Point (pI)

25.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 3 - 124 3.1e-28 Transmembrane amino acid transporter protein
Aa_trans PF01490 125 - 208 3.6e-15 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 427
AccB7I CCANNNNNTGG 2 cut(s) 46, 330
AccI GTMKAC 1 cut(s) 345
AciI CCGC 2 cut(s) 294, 341
AclI AACGTT 1 cut(s) 25
AclWI GGATC 1 cut(s) 637
AcoI YGGCCR 2 cut(s) 291, 576
AcsI RAATTY 1 cut(s) 517
AdeI CACNNNGTG 2 cut(s) 180, 244
AfaI GTAC 2 cut(s) 134, 429
AfiI CCNNNNNNNGG 3 cut(s) 46, 330, 605
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 3 cut(s) 113, 268, 522
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 3 cut(s) 73, 299, 392
AluI AGCT 3 cut(s) 73, 299, 392
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 1 cut(s) 637
AoxI GGCC 3 cut(s) 92, 291, 576
ApeKI GCWGC 3 cut(s) 157, 296, 338
ApoI RAATTY 1 cut(s) 517
Asp718I GGTACC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 92, 166
AsuHPI GGTGA 3 cut(s) 77, 133, 631
AvaII GGWCC 1 cut(s) 166
BaeI ACNNNNGTAYC 2 cut(s) 419, 452
BalI TGGCCA 1 cut(s) 578
BanI GGYRCC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 185
BbvCI CCTCAGC 1 cut(s) 96
BbvI GCAGC 3 cut(s) 144, 308, 325
BccI CCATC 2 cut(s) 4, 281
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 158
BisI GCNGC 4 cut(s) 158, 294, 297, 339
BlsI GCNGC 4 cut(s) 159, 295, 298, 340
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 92, 166
BmiI GGNNCC 3 cut(s) 15, 93, 429
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 1 cut(s) 612
BmuI ACTGGG 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 331
BsaJI CCNNGG 1 cut(s) 423
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 6 cut(s) 254, 260, 284, 290, 435, 465
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 330, 605
Bse1I ACTGG 2 cut(s) 17, 485
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 3 cut(s) 46, 330, 605
BseMII CTCAG 3 cut(s) 110, 198, 402
BseNI ACTGG 2 cut(s) 17, 485
BseXI GCAGC 3 cut(s) 144, 308, 325
BshFI GGCC 3 cut(s) 94, 293, 578
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 144, 279
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 3 cut(s) 46, 330, 605
BsmAI GTCTC 1 cut(s) 547
BsmBI CGTCTC 1 cut(s) 547
BsmFI GGGAC 1 cut(s) 581
BsnI GGCC 3 cut(s) 94, 293, 578
Bsp1286I GDGCHC 1 cut(s) 185
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 4 cut(s) 31, 198, 222, 629
BspACI CCGC 2 cut(s) 294, 341
BspANI GGCC 3 cut(s) 94, 293, 578
BspCNI CTCAG 3 cut(s) 109, 197, 403
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 15, 93, 429
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 1 cut(s) 637
BspT107I GGYRCC 1 cut(s) 427
BsrGI TGTACA 1 cut(s) 132
BsrI ACTGG 2 cut(s) 17, 485
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 4 cut(s) 31, 198, 222, 629
BssT1I CCWWGG 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 96, 184, 411
BstEII GGTNACC 1 cut(s) 139
BstF5I GGATG 1 cut(s) 534
BstKTI GATC 4 cut(s) 34, 201, 225, 632
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 4 cut(s) 31, 198, 222, 629
BstMWI GCNNNNNNNGC 2 cut(s) 189, 335
BstNSI RCATGY 2 cut(s) 59, 586
BstPI GGTNACC 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 144, 308, 325
BsuRI GGCC 3 cut(s) 94, 293, 578
BtsCI GGATG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 24, 174
Cac8I GCNNGC 1 cut(s) 57
CciI TCATGA 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 92, 166
Csp6I GTAC 2 cut(s) 133, 428
CviAII CATG 9 cut(s) 5, 41, 56, 136, 170, 220, 304, 330, 583
CviJI RGCY 9 cut(s) 73, 94, 293, 299, 392, 418, 470, 565, 578
CviKI_1 RGCY 9 cut(s) 73, 94, 293, 299, 392, 418, 470, 565, 578
CviQI GTAC 2 cut(s) 133, 428
DdeI CTNAG 3 cut(s) 96, 184, 411
DpnI GATC 4 cut(s) 33, 200, 224, 631
DpnII GATC 4 cut(s) 31, 198, 222, 629
DraIII CACNNNGTG 2 cut(s) 180, 244
EaeI YGGCCR 2 cut(s) 291, 576
Eco130I CCWWGG 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 166
Eco91I GGTNACC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 92
EcoO65I GGTNACC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 2 cut(s) 6, 61
ErhI CCWWGG 1 cut(s) 423
Esp3I CGTCTC 1 cut(s) 547
FaeI CATG 9 cut(s) 8, 44, 59, 139, 173, 223, 307, 333, 586
FaqI GGGAC 1 cut(s) 581
FatI CATG 9 cut(s) 4, 40, 55, 135, 169, 219, 303, 329, 582
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 158, 294, 297, 339
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 4 cut(s) 158, 294, 297, 339
FspBI CTAG 1 cut(s) 494
GluI GCNGC 4 cut(s) 158, 294, 297, 339
HaeIII GGCC 3 cut(s) 94, 293, 578
HapII CCGG 2 cut(s) 144, 279
Hin1II CATG 9 cut(s) 8, 44, 59, 139, 173, 223, 307, 333, 586
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 444
HpaII CCGG 2 cut(s) 144, 279
HphI GGTGA 3 cut(s) 77, 133, 631
Hpy166II GTNNAC 3 cut(s) 133, 238, 346
Hpy188I TCNGA 1 cut(s) 187
Hpy188III TCNNGA 1 cut(s) 220
Hpy8I GTNNAC 3 cut(s) 133, 238, 346
HpyAV CCTTC 4 cut(s) 157, 266, 411, 429
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 6 cut(s) 4, 59, 126, 160, 192, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 335
HpyF3I CTNAG 3 cut(s) 96, 184, 411
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 9 cut(s) 8, 44, 59, 139, 173, 223, 307, 333, 586
KpnI GGTACC 1 cut(s) 431
Kzo9I GATC 4 cut(s) 31, 198, 222, 629
LpnPI CCDG 7 cut(s) 30, 157, 292, 423, 498, 602, 612
Lsp1109I GCAGC 3 cut(s) 144, 308, 325
LweI GCATC 1 cut(s) 612
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 3 cut(s) 35, 139, 314
MalI GATC 4 cut(s) 33, 200, 224, 631
MboI GATC 4 cut(s) 31, 198, 222, 629
MhlI GDGCHC 1 cut(s) 185
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 6 cut(s) 44, 203, 396, 462, 517, 567
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 1 cut(s) 453
MnlI CCTC 6 cut(s) 105, 140, 241, 389, 548, 627
Mox20I TGGCCA 1 cut(s) 578
Mph1103I ATGCAT 2 cut(s) 6, 61
MscI TGGCCA 1 cut(s) 578
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 144, 279
MwoI GCNNNNNNNGC 2 cut(s) 189, 335
NdeII GATC 4 cut(s) 31, 198, 222, 629
NlaIII CATG 9 cut(s) 8, 44, 59, 139, 173, 223, 307, 333, 586
NlaIV GGNNCC 3 cut(s) 15, 93, 429
NmuCI GTSAC 1 cut(s) 139
NsiI ATGCAT 2 cut(s) 6, 61
NspI RCATGY 2 cut(s) 59, 586
PaeI GCATGC 1 cut(s) 59
PagI TCATGA 1 cut(s) 219
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PflMI CCANNNNNTGG 2 cut(s) 46, 330
PkrI GCNGC 4 cut(s) 159, 295, 298, 340
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PscI ACATGT 1 cut(s) 582
Psp1406I AACGTT 1 cut(s) 25
PspEI GGTNACC 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 15, 93, 429
PspPI GGNCC 2 cut(s) 92, 166
PstI CTGCAG 1 cut(s) 162
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 2 cut(s) 134, 429
RsaNI GTAC 2 cut(s) 133, 428
RseI CAYNNNNRTG 1 cut(s) 464
SatI GCNGC 4 cut(s) 158, 294, 297, 339
Sau3AI GATC 4 cut(s) 31, 198, 222, 629
Sau96I GGNCC 2 cut(s) 92, 166
SchI GAGTC 1 cut(s) 453
SduI GDGCHC 1 cut(s) 185
SfaNI GCATC 1 cut(s) 612
SfcI CTRYAG 1 cut(s) 158
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
SphI GCATGC 1 cut(s) 59
Sse9I AATT 6 cut(s) 44, 203, 396, 462, 517, 567
SsiI CCGC 2 cut(s) 294, 341
SspMI CTAG 1 cut(s) 494
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 28
TaqI TCGA 3 cut(s) 369, 381, 632
TasI AATT 6 cut(s) 44, 203, 396, 462, 517, 567
TatI WGTACW 1 cut(s) 132
TauI GCSGC 1 cut(s) 296
TscAI CASTG 2 cut(s) 24, 181
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 157, 296, 338
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 57, 205
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 2 cut(s) 24, 181
Van91I CCANNNNNTGG 2 cut(s) 46, 330
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 1 cut(s) 517
XceI RCATGY 2 cut(s) 59, 586
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 494
Zsp2I ATGCAT 2 cut(s) 6, 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.