Rmu_sc0001616.1_g000044

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001616.1
N/A
Physical Location & Seq
Reverse (-)
217827 .. 220795
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
atgttaacaggttctgcgaagcaagtggaattgttggctattggagagggccttgatcttgcaaggcaaatgcagctatcccaggtgattcttgaaacggattgccttgttgcagtgcaggacattgcccaaaatgctggttctgatttggctagtattgttgatgacattttggaggttcataaactaataccgcaggttcaactcacttttgtgcctagaacatgcaaccggctagcacataggcctgtctacattaatcaccaaggctgtgaatataagctgcattacgggtcttcaaatggctccacaagcaccaaagctgcaccacttgttcttgatagttttctatttggcactgctaatgaggtagagtttactcactacaagaagcttcttgacacgtatgagaaagcttcagggcataaggtaaattaccagaaaagtagtgtgatgtttagcaataatatggagaaatccaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

161

Amino Acids

17.82

Weight (kDa)

6.28

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 5 - 81 1.9e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 252
AciI CCGC 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 402
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 95, 203, 300
AjnI CCWGG 1 cut(s) 81
AleI CACNNNNGTG 1 cut(s) 212
AluBI AGCT 5 cut(s) 76, 283, 323, 394, 416
AluI AGCT 5 cut(s) 76, 283, 323, 394, 416
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 2 cut(s) 49, 245
ApeKI GCWGC 3 cut(s) 73, 283, 323
AseI ATTAAT 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 97, 254
AsuNHI GCTAGC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 288
BbvI GCAGC 3 cut(s) 85, 270, 310
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 3 cut(s) 153, 219, 236
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 3 cut(s) 74, 284, 324
BlsI GCNGC 3 cut(s) 75, 285, 325
Bme1390I CCNGG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 83
BmtI GCTAGC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 405
BsaJI CCNNGG 2 cut(s) 81, 265
Bse118I RCCGGY 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 2 cut(s) 81, 265
BseMI GCAATG 1 cut(s) 123
BseXI GCAGC 3 cut(s) 85, 270, 310
BsgI GTGCAG 2 cut(s) 137, 309
BshFI GGCC 2 cut(s) 51, 247
BsiSI CCGG 1 cut(s) 232
BsnI GGCC 2 cut(s) 51, 247
Bsp143I GATC 1 cut(s) 55
BspACI CCGC 1 cut(s) 194
BspANI GGCC 2 cut(s) 51, 247
BspLI GGNNCC 1 cut(s) 307
BspMI ACCTGC 1 cut(s) 187
BspOI GCTAGC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 231
BssECI CCNNGG 2 cut(s) 81, 265
BssMI GATC 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 237
BstKTI GATC 1 cut(s) 58
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 3 cut(s) 73, 134, 312
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 81
BstV1I GCAGC 3 cut(s) 85, 270, 310
BstV2I GAAGAC 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 137
BsuRI GGCC 2 cut(s) 51, 247
BtsI GCAGTG 2 cut(s) 120, 357
BtsIMutI CAGTG 2 cut(s) 120, 357
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 14
Cfr10I RCCGGY 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 49
CviAII CATG 1 cut(s) 225
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 1 cut(s) 228
FaiI YATR 7 cut(s) 183, 226, 243, 279, 408, 426, 470
FatI CATG 1 cut(s) 224
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 3 cut(s) 74, 284, 324
Fsp4HI GCNGC 3 cut(s) 74, 284, 324
FspBI CTAG 3 cut(s) 153, 219, 236
GluI GCNGC 3 cut(s) 74, 284, 324
HaeIII GGCC 2 cut(s) 51, 247
HapII CCGG 1 cut(s) 232
Hin1II CATG 1 cut(s) 228
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 392, 414
HinfI GANTC 1 cut(s) 88
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 1 cut(s) 232
HphI GGTGA 2 cut(s) 97, 254
Hpy166II GTNNAC 3 cut(s) 6, 253, 378
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 3 cut(s) 92, 338, 398
Hpy8I GTNNAC 3 cut(s) 6, 253, 378
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 7 cut(s) 62, 73, 113, 118, 228, 286, 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 134, 312
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 9 cut(s) 68, 95, 104, 123, 182, 245, 261, 405, 452
Lsp1109I GCAGC 3 cut(s) 85, 270, 310
MaeI CTAG 3 cut(s) 153, 219, 236
MaeII ACGT 1 cut(s) 404
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 1 cut(s) 288
MluCI AATT 2 cut(s) 29, 433
MnlI CCTC 3 cut(s) 40, 169, 361
MseI TTAA 2 cut(s) 5, 258
MslI CAYNNNNRTG 1 cut(s) 212
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 73, 134, 312
NdeII GATC 1 cut(s) 55
NheI GCTAGC 1 cut(s) 235
NlaIII CATG 1 cut(s) 228
NlaIV GGNNCC 1 cut(s) 307
NspI RCATGY 1 cut(s) 228
OliI CACNNNNGTG 1 cut(s) 212
PceI AGGCCT 1 cut(s) 247
PfeI GAWTC 1 cut(s) 88
PkrI GCNGC 3 cut(s) 75, 285, 325
Ppu21I YACGTR 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 14
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 5, 258
SatI GCNGC 3 cut(s) 74, 284, 324
Sau3AI GATC 1 cut(s) 55
Sau96I GGNCC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 212
Sse9I AATT 2 cut(s) 29, 433
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 1 cut(s) 194
SspMI CTAG 3 cut(s) 153, 219, 236
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 265
TaiI ACGT 1 cut(s) 407
TasI AATT 2 cut(s) 29, 433
TfiI GAWTC 1 cut(s) 88
Tru1I TTAA 2 cut(s) 5, 258
Tru9I TTAA 2 cut(s) 5, 258
TscAI CASTG 2 cut(s) 120, 364
TseI GCWGC 3 cut(s) 73, 283, 323
TspDTI ATGAA 1 cut(s) 170
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 120, 364
VspI ATTAAT 1 cut(s) 258
XceI RCATGY 1 cut(s) 228
XmiI GTMKAC 1 cut(s) 252
XspI CTAG 3 cut(s) 153, 219, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.