Rmu_sc0001725.1_g000008

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001725.1
N/A
Physical Location & Seq
Forward (+)
27540 .. 31270
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
atgtggataggaaatttggggaacattctcctcattataatcccagcagtttgtgatcaattagggagcccttttggggatgctgacacctgtgatacttatggagtcgcttatgcttcactttcaatggcgatatcttcaaagcctagcactccagatgctatccagtcttctgagactctgcctctgcttacttccatagaagatgacaatgtggatcaatatgcacgtactgtatctaaaggagaacctaagatgacatgtctaggtaagaccaaggaatggatggtgatggtgaataaaaagctcaatttgaaatcgatatttgcggcttcaactattgcagcgattgctgggtttgtaattggatttatccctcagattcgaaacttgttgatagcagacgacgcttctctagcagttatcgtttactctacttccttgttgggagacgcagctatcccacttctcactttgattataggaggaaacctcattaaaggtttgagagtgtcagggatacagaaatccatcattgctggcatcgtggttgttaaatatgttgcgctgcctcttactggagtactgattgttaaattaagagcattaaaactaggcttagtgaccaatgatccatttgtttgttcttctgcttcagttttcagtcccgcctgcaatgaacacggccctagcctcaagctcttgcctgctgctcctcgcgttgcagccctgccctgcagcctctttccgcaagtccttgccagcctaccgcgaccctgcaccagccctctagcgtccctcgccttgcagtcctgccgcactgctggtgccgtacctgctgctgctgcagaaactgccgaacttgctgctgctgaaattgttgttgccaactgctgctaccgcatactaccattgccactgctgttcactgataccaactgttgctgctgctcgctgataccaattgttgctgctgccctaacacgcctgctccaacacgcgcgtgttctcaagccccaaatcatcaagatgtcaaatgaacctagactcgactttcccacgcttgactcaacaggctcggattaccatagctgggtaaccgatattgagaaccacctcacttcaaagggaatattacccataatccaggaacctaacccgaatctcgtgttcacacgaacacctacaaagcatgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

399

Amino Acids

42.2

Weight (kDa)

7.88

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 3 - 256 2.6e-21 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 38
Acc36I ACCTGC 1 cut(s) 844
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 1 cut(s) 282
AccII CGCG 4 cut(s) 720, 772, 1001, 1003
AciI CCGC 6 cut(s) 329, 669, 749, 770, 817, 901
AclWI GGATC 2 cut(s) 225, 626
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 3 cut(s) 232, 585, 834
AfiI CCNNNNNNNGG 4 cut(s) 76, 282, 578, 1093
AflIII ACRYGT 1 cut(s) 260
AgsI TTSAA 5 cut(s) 126, 141, 316, 336, 1125
AjnI CCWGG 1 cut(s) 1146
AleI CACNNNNGTG 1 cut(s) 1002
AluBI AGCT 4 cut(s) 307, 458, 700, 1092
AluI AGCT 4 cut(s) 307, 458, 700, 1092
Alw26I GTCTC 2 cut(s) 170, 444
AlwI GGATC 2 cut(s) 225, 626
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 1 cut(s) 685
ApoI RAATTY 1 cut(s) 13
AspLEI GCGC 2 cut(s) 568, 1003
AspS9I GGNCC 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 301, 307
AsuII TTCGAA 1 cut(s) 385
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 71
BauI CACGAG 1 cut(s) 1166
BbsI GAAGAC 1 cut(s) 162
BccI CCATC 3 cut(s) 280, 286, 539
BceAI ACGGC 2 cut(s) 700, 815
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 1 cut(s) 1148
BciVI GTATCC 1 cut(s) 513
BclI TGATCA 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 170, 444
BfaI CTAG 7 cut(s) 147, 266, 416, 614, 690, 791, 1044
BfmI CTRYAG 2 cut(s) 736, 846
BfuAI ACCTGC 1 cut(s) 844
BfuI GTATCC 1 cut(s) 513
BmcAI AGTACT 1 cut(s) 585
Bme1390I CCNGG 1 cut(s) 1148
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 3 cut(s) 68, 829, 1152
BmrFI CCNGG 1 cut(s) 1148
BmsI GCATC 3 cut(s) 70, 148, 552
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 477, 509
BpmI CTGGAG 2 cut(s) 138, 600
Bpu14I TTCGAA 1 cut(s) 385
BpuEI CTTGAG 2 cut(s) 680, 995
Bsa29I ATCGAT 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 975, 1005
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 282, 578, 1093
Bse1I ACTGG 2 cut(s) 166, 583
Bse3DI GCAATG 3 cut(s) 534, 682, 911
BseBI CCWGG 1 cut(s) 1148
BseCI ATCGAT 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 85, 291
BseLI CCNNNNNNNGG 4 cut(s) 76, 282, 578, 1093
BseMI GCAATG 3 cut(s) 534, 682, 911
BseMII CTCAG 2 cut(s) 165, 392
BseNI ACTGG 2 cut(s) 166, 583
BseRI GAGGAG 2 cut(s) 20, 705
BseYI CCCAGC 3 cut(s) 43, 353, 1092
BsgI GTGCAG 1 cut(s) 763
Bsh1236I CGCG 4 cut(s) 720, 772, 1001, 1003
BshFI GGCC 1 cut(s) 687
BshNI GGYRCC 1 cut(s) 827
BshVI ATCGAT 1 cut(s) 320
BslFI GGGAC 2 cut(s) 651, 781
BslI CCNNNNNNNGG 4 cut(s) 76, 282, 578, 1093
BsmAI GTCTC 2 cut(s) 170, 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 651, 781
BsnI GGCC 1 cut(s) 687
Bsp119I TTCGAA 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 55, 217, 631
BspACI CCGC 6 cut(s) 329, 669, 749, 770, 817, 901
BspANI GGCC 1 cut(s) 687
BspCNI CTCAG 2 cut(s) 166, 391
BspDI ATCGAT 1 cut(s) 320
BspFNI CGCG 4 cut(s) 720, 772, 1001, 1003
BspLI GGNNCC 3 cut(s) 68, 829, 1152
BspMAI CTGCAG 2 cut(s) 740, 850
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 2 cut(s) 225, 626
BspT104I TTCGAA 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 827
BsrDI GCAATG 3 cut(s) 534, 682, 911
BsrI ACTGG 2 cut(s) 166, 583
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 55, 217, 631
BssSI CACGAG 1 cut(s) 1166
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 1166
Bst2UI CCWGG 1 cut(s) 1148
Bst4CI ACNGT 2 cut(s) 235, 941
BstAPI GCANNNNNTGC 2 cut(s) 350, 854
BstBAI YACGTR 1 cut(s) 230
BstBI TTCGAA 1 cut(s) 385
BstC8I GCNNGC 7 cut(s) 541, 673, 708, 763, 953, 989, 1194
BstDEI CTNAG 4 cut(s) 174, 252, 378, 619
BstEII GGTNACC 1 cut(s) 1096
BstF5I GGATG 2 cut(s) 85, 291
BstFNI CGCG 4 cut(s) 720, 772, 1001, 1003
BstHHI GCGC 2 cut(s) 568, 1003
BstKTI GATC 3 cut(s) 58, 220, 634
BstMAI GTCTC 2 cut(s) 170, 444
BstMBI GATC 3 cut(s) 55, 217, 631
BstMWI GCNNNNNNNGC 9 cut(s) 350, 407, 416, 800, 836, 845, 854, 863, 900
BstNI CCWGG 1 cut(s) 1148
BstNSI RCATGY 2 cut(s) 264, 1196
BstPI GGTNACC 1 cut(s) 1096
BstSCI CCNGG 1 cut(s) 1146
BstSFI CTRYAG 2 cut(s) 736, 846
BstUI CGCG 4 cut(s) 720, 772, 1001, 1003
BstV2I GAAGAC 1 cut(s) 162
Bsu15I ATCGAT 1 cut(s) 320
BsuI GTATCC 1 cut(s) 513
BsuRI GGCC 1 cut(s) 687
BsuTUI ATCGAT 1 cut(s) 320
BtsCI GGATG 2 cut(s) 85, 291
BtsI GCAGTG 2 cut(s) 819, 917
BtsIMutI CAGTG 3 cut(s) 819, 917, 927
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 7 cut(s) 541, 673, 708, 763, 953, 989, 1194
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 2 cut(s) 568, 1003
Cfr13I GGNCC 1 cut(s) 686
ClaI ATCGAT 1 cut(s) 320
CseI GACGC 3 cut(s) 416, 461, 783
Csp6I GTAC 3 cut(s) 231, 584, 833
CviAII CATG 2 cut(s) 261, 1193
CviQI GTAC 3 cut(s) 231, 584, 833
DdeI CTNAG 4 cut(s) 174, 252, 378, 619
DpnI GATC 3 cut(s) 57, 219, 633
DpnII GATC 3 cut(s) 55, 217, 631
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 1096
EcoO65I GGTNACC 1 cut(s) 1096
EcoRII CCWGG 1 cut(s) 1146
EcoRV GATATC 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 2 cut(s) 264, 1196
FaqI GGGAC 2 cut(s) 651, 781
FatI CATG 2 cut(s) 260, 1192
FauI CCCGC 1 cut(s) 676
FbaI TGATCA 1 cut(s) 55
FokI GGATG 2 cut(s) 92, 298
FriOI GRGCYC 1 cut(s) 71
FspBI CTAG 7 cut(s) 147, 266, 416, 614, 690, 791, 1044
GlaI GCGC 2 cut(s) 567, 1002
GsaI CCCAGC 3 cut(s) 47, 357, 1096
GsuI CTGGAG 2 cut(s) 138, 600
HaeIII GGCC 1 cut(s) 687
HgaI GACGC 3 cut(s) 416, 461, 783
HhaI GCGC 2 cut(s) 568, 1003
Hin1II CATG 2 cut(s) 264, 1196
Hin6I GCGC 2 cut(s) 566, 1001
HinP1I GCGC 2 cut(s) 566, 1001
HinfI GANTC 6 cut(s) 105, 178, 382, 1047, 1067, 1162
HphI GGTGA 2 cut(s) 301, 307
Hpy166II GTNNAC 3 cut(s) 430, 927, 1173
Hpy188I TCNGA 3 cut(s) 175, 381, 1081
Hpy188III TCNNGA 2 cut(s) 155, 1027
Hpy8I GTNNAC 3 cut(s) 430, 927, 1173
Hpy99I CGWCG 1 cut(s) 410
HpyCH4III ACNGT 2 cut(s) 235, 941
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 8 cut(s) 227, 344, 675, 725, 738, 780, 808, 848
HpyF10VI GCNNNNNNNGC 9 cut(s) 350, 407, 416, 800, 836, 845, 854, 863, 900
HpyF3I CTNAG 4 cut(s) 174, 252, 378, 619
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 2 cut(s) 264, 1196
HspAI GCGC 2 cut(s) 566, 1001
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 3 cut(s) 55, 217, 631
LmnI GCTCC 3 cut(s) 66, 718, 996
LweI GCATC 3 cut(s) 70, 148, 552
MaeI CTAG 7 cut(s) 147, 266, 416, 614, 690, 791, 1044
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 2 cut(s) 622, 1096
MalI GATC 3 cut(s) 57, 219, 633
MboI GATC 3 cut(s) 55, 217, 631
MboII GAAGA 4 cut(s) 129, 162, 215, 639
MfeI CAATTG 1 cut(s) 963
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 7 cut(s) 13, 59, 310, 363, 596, 876, 963
MlyI GAGTC 4 cut(s) 114, 172, 1041, 1061
MmeI TCCRAC 1 cut(s) 1018
MseI TTAA 6 cut(s) 498, 555, 594, 599, 608, 1198
MslI CAYNNNNRTG 2 cut(s) 1002, 1028
MspR9I CCNGG 1 cut(s) 1148
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 1 cut(s) 1148
MvnI CGCG 4 cut(s) 720, 772, 1001, 1003
MwoI GCNNNNNNNGC 9 cut(s) 350, 407, 416, 800, 836, 845, 854, 863, 900
NdeII GATC 3 cut(s) 55, 217, 631
NlaIII CATG 2 cut(s) 264, 1196
NlaIV GGNNCC 3 cut(s) 68, 829, 1152
NmuCI GTSAC 1 cut(s) 622
NspI RCATGY 2 cut(s) 264, 1196
NspV TTCGAA 1 cut(s) 385
OliI CACNNNNGTG 1 cut(s) 1002
PaeI GCATGC 1 cut(s) 1196
PciI ACATGT 1 cut(s) 260
PfeI GAWTC 2 cut(s) 382, 1162
PflMI CCANNNNNTGG 1 cut(s) 282
PfoI TCCNGGA 1 cut(s) 1146
PleI GAGTC 4 cut(s) 113, 172, 1041, 1061
PpsI GAGTC 4 cut(s) 113, 172, 1041, 1061
Ppu21I YACGTR 1 cut(s) 230
PscI ACATGT 1 cut(s) 260
PsiI TTATAA 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 1146
PspEI GGTNACC 1 cut(s) 1096
PspFI CCCAGC 3 cut(s) 43, 353, 1092
PspGI CCWGG 1 cut(s) 1146
PspN4I GGNNCC 3 cut(s) 68, 829, 1152
PspPI GGNCC 1 cut(s) 686
PstI CTGCAG 2 cut(s) 740, 850
PstNI CAGNNNCTG 1 cut(s) 854
RsaI GTAC 3 cut(s) 232, 585, 834
RsaNI GTAC 3 cut(s) 231, 584, 833
RseI CAYNNNNRTG 2 cut(s) 1002, 1028
SaqAI TTAA 6 cut(s) 498, 555, 594, 599, 608, 1198
Sau3AI GATC 3 cut(s) 55, 217, 631
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 4 cut(s) 114, 172, 1041, 1061
ScrFI CCNGG 1 cut(s) 1148
SduI GDGCHC 1 cut(s) 71
SfaNI GCATC 3 cut(s) 70, 148, 552
SfcI CTRYAG 2 cut(s) 736, 846
SfuI TTCGAA 1 cut(s) 385
SmiMI CAYNNNNRTG 2 cut(s) 1002, 1028
SmlI CTYRAG 2 cut(s) 695, 1010
SmoI CTYRAG 2 cut(s) 695, 1010
SphI GCATGC 1 cut(s) 1196
Sse9I AATT 7 cut(s) 13, 59, 310, 363, 596, 876, 963
SsiI CCGC 6 cut(s) 329, 669, 749, 770, 817, 901
SspI AATATT 1 cut(s) 1134
SspMI CTAG 7 cut(s) 147, 266, 416, 614, 690, 791, 1044
StyD4I CCNGG 1 cut(s) 1146
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 2 cut(s) 235, 941
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 320, 385, 1050
TasI AATT 7 cut(s) 13, 59, 310, 363, 596, 876, 963
TatI WGTACW 1 cut(s) 583
TauI GCSGC 2 cut(s) 332, 819
TfiI GAWTC 2 cut(s) 382, 1162
Tru1I TTAA 6 cut(s) 498, 555, 594, 599, 608, 1198
Tru9I TTAA 6 cut(s) 498, 555, 594, 599, 608, 1198
TscAI CASTG 3 cut(s) 826, 924, 934
TseFI GTSAC 1 cut(s) 622
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 2 cut(s) 693, 1053
TspRI CASTG 3 cut(s) 826, 924, 934
Van91I CCANNNNNTGG 1 cut(s) 282
XapI RAATTY 1 cut(s) 13
XceI RCATGY 2 cut(s) 264, 1196
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XspI CTAG 7 cut(s) 147, 266, 416, 614, 690, 791, 1044
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.