Rmu_sc0001777.1_g000003

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001777.1
N/A
Physical Location & Seq
Reverse (-)
7236 .. 7868
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001777.1_g000003.1.cds

Sequence Viewer

Length: 504 bp
atgcacaaattaagatggttgtgctggcatgagtttcctcttgacttcataccaaaggggtttatgcaacttgctattgaccttcaatatagcaacatcacacaagcttggaaggattcagagttacttgagaagctgaagattcttaatctcagccactgcccttacataacacaaactccagactttttgaaattcccaaatctggagcagttgatacttgaagactgtgagtgtttgtctgaggttcacgagtccattggagctcttaaagcactagctttggtaaatctgcgggactgcaagatgattaaggatctcccaaggagtttctacaagttgaaatctgttgatactgttattctctcaggttgttcaaagtttgaaaccccggcggtggacttgaggaacatggtatcactgacaaccaatgttttcaaatgcgaaagcgaaggcgaaggcgaccacctagagcctcgcgaggcgagtgcctcaaggcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

167

Amino Acids

19.21

Weight (kDa)

5.67

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 480
AciI CCGC 2 cut(s) 295, 395
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 158
AfiI CCNNNNNNNGG 2 cut(s) 205, 397
AgsI TTSAA 7 cut(s) 86, 193, 224, 343, 378, 386, 439
AluBI AGCT 4 cut(s) 107, 136, 266, 281
AluI AGCT 4 cut(s) 107, 136, 266, 281
Alw21I GWGCWC 1 cut(s) 268
AlwI GGATC 1 cut(s) 324
AlwNI CAGNNNCTG 1 cut(s) 159
ApoI RAATTY 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 392
BanII GRGCYC 1 cut(s) 268
BauI CACGAG 1 cut(s) 251
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 268
BccI CCATC 1 cut(s) 9
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BcnI CCSGG 1 cut(s) 392
BfaI CTAG 2 cut(s) 278, 470
Bme1390I CCNGG 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 2 cut(s) 165, 227
BpuEI CTTGAG 3 cut(s) 149, 424, 478
BpuMI CCSGG 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 323, 390
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 397
BseDI CCNNGG 2 cut(s) 323, 390
BseLI CCNNNNNNNGG 2 cut(s) 205, 397
BseMII CTCAG 3 cut(s) 166, 234, 381
Bsh1236I CGCG 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 205, 397
BsmFI GGGAC 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 316
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 2 cut(s) 295, 395
BspCNI CTCAG 3 cut(s) 165, 235, 380
BspFNI CGCG 1 cut(s) 480
BspPI GGATC 1 cut(s) 324
BssECI CCNNGG 2 cut(s) 323, 390
BssMI GATC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 251
Bst4CI ACNGT 2 cut(s) 230, 358
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 152, 243, 367
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 1 cut(s) 319
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 390
BstUI CGCG 1 cut(s) 480
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 157, 419
BtuMI TCGCGA 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 26
CaiI CAGNNNCTG 1 cut(s) 159
CviAII CATG 2 cut(s) 29, 412
CviJI RGCY 6 cut(s) 107, 136, 156, 266, 281, 475
CviKI_1 RGCY 6 cut(s) 107, 136, 156, 266, 281, 475
DdeI CTNAG 3 cut(s) 152, 243, 367
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 1 cut(s) 323
Eco24I GRGCYC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 2 cut(s) 32, 415
FaiI YATR 6 cut(s) 30, 50, 65, 90, 170, 413
FaqI GGGAC 1 cut(s) 311
FatI CATG 2 cut(s) 28, 411
FauI CCCGC 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 268
FspBI CTAG 2 cut(s) 278, 470
GsuI CTGGAG 2 cut(s) 165, 227
HapII CCGG 1 cut(s) 392
Hin1II CATG 2 cut(s) 32, 415
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 3 cut(s) 116, 142, 254
HpaII CCGG 1 cut(s) 392
Hpy166II GTNNAC 2 cut(s) 250, 400
Hpy188I TCNGA 2 cut(s) 121, 244
Hpy188III TCNNGA 5 cut(s) 41, 182, 206, 251, 479
Hpy8I GTNNAC 2 cut(s) 250, 400
HpyAV CCTTC 4 cut(s) 92, 106, 446, 452
HpyCH4III ACNGT 2 cut(s) 230, 358
HpyCH4V TGCA 3 cut(s) 4, 67, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 3 cut(s) 152, 243, 367
Hsp92II CATG 2 cut(s) 32, 415
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 2 cut(s) 208, 263
LpnPI CCDG 5 cut(s) 10, 191, 195, 354, 405
MaeI CTAG 2 cut(s) 278, 470
MaeIII GTNAC 1 cut(s) 123
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 2 cut(s) 151, 236
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 2 cut(s) 8, 194
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 6 cut(s) 48, 238, 399, 475, 486, 502
MseI TTAA 4 cut(s) 11, 147, 270, 312
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 1 cut(s) 272
NciI CCSGG 1 cut(s) 392
NdeII GATC 1 cut(s) 316
NlaIII CATG 2 cut(s) 32, 415
NruI TCGCGA 1 cut(s) 480
PfeI GAWTC 2 cut(s) 116, 142
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 159
PsuI RGATCY 1 cut(s) 316
RruI TCGCGA 1 cut(s) 480
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 4 cut(s) 11, 147, 270, 312
Sau3AI GATC 1 cut(s) 316
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 1 cut(s) 268
SetI ASST 8 cut(s) 84, 109, 138, 249, 268, 283, 373, 471
SmlI CTYRAG 3 cut(s) 128, 403, 493
SmoI CTYRAG 3 cut(s) 128, 403, 493
Sse9I AATT 2 cut(s) 8, 194
SsiI CCGC 2 cut(s) 295, 395
SspMI CTAG 2 cut(s) 278, 470
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 230, 358
TasI AATT 2 cut(s) 8, 194
TfiI GAWTC 2 cut(s) 116, 142
Tru1I TTAA 4 cut(s) 11, 147, 270, 312
Tru9I TTAA 4 cut(s) 11, 147, 270, 312
TscAI CASTG 2 cut(s) 164, 426
TspDTI ATGAA 1 cut(s) 37
TspRI CASTG 2 cut(s) 164, 426
XapI RAATTY 1 cut(s) 194
XspI CTAG 2 cut(s) 278, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.