Rmu_sc0001785.1_g000016

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001785.1
N/A
Physical Location & Seq
Forward (+)
59644 .. 61222
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 945 bp
atgcgccaccaacagtggcagcgcgtgggccccacgcgccggttgccaccaaattttggcagcaccacctactcaacagacccatcatttttcacaactacaacaagttccaattttaccttgaagggctccaatttggccggtgaaatttttccagaaaactccaatgcgccaccaacagtggcagcgcgtgggccccaagcgccggtggccaccaaattttggcagcaccacctactcaacagacccatcatttttcacaactacaaaaagttccaattttaccttgaagggctccaatttggccgtaaccttcacagctcaaaatcgagctcttccggccaaatcgctgcaaaacaccaccacagacgtgaccagggtgaagaggcgagccagctgatgcccggattccatcgtggggtggccggaggaggaagaaatcggagggagaagaaattgggccgaagaggagaaaggatcgggggagaagagagaaaaaccgcaaatagagcagccttagaggcaaaaaaggcgaaagccattatcctaatgactcgtcatatggatgatttgctccagtacgagtgtaagaatgaagaagaccccagaaggctgtgggtctcacttgaagaaagatttggcaaagtccgtgactccctgcttcctgacctagaagtgagatggcatagtctccgcttctgtgatttcaagtcagttcttgactacaactcagaagcatttcgcattaaatccttaatggaattctgtggtaaagagatcacatatgcgatgttgattgagaatactctctctacctttcccgtctttgcattggtggttgctaagaactatcaaatcgatgttacttcagaacgaatcataaggtttcatgagctcatattggagctatggatgtcgctgaaaagcaagacaacatccttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

314

Amino Acids

36.14

Weight (kDa)

9.85

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 56, 222
AccII CGCG 3 cut(s) 24, 37, 190
AciI CCGC 2 cut(s) 501, 694
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 5 cut(s) 138, 210, 304, 340, 423
AcsI RAATTY 4 cut(s) 52, 147, 218, 761
AcuI CTGAAG 1 cut(s) 852
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 5 cut(s) 39, 56, 205, 222, 418
AgsI TTSAA 4 cut(s) 124, 290, 629, 709
AjiI CACGTC 1 cut(s) 371
AjnI CCWGG 1 cut(s) 375
AjuI GAANNNNNNNTTGG 2 cut(s) 621, 653
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 5 cut(s) 321, 333, 397, 895, 907
AluI AGCT 5 cut(s) 321, 333, 397, 895, 907
Alw21I GWGCWC 2 cut(s) 335, 897
Alw26I GTCTC 2 cut(s) 625, 695
AlwI GGATC 1 cut(s) 485
AoxI GGCC 8 cut(s) 28, 138, 194, 210, 304, 340, 423, 460
ApaI GGGCCC 2 cut(s) 32, 198
ApeKI GCWGC 6 cut(s) 19, 60, 185, 226, 350, 512
ApoI RAATTY 4 cut(s) 52, 147, 218, 761
Asp700I GAANNNNTTC 2 cut(s) 150, 738
AspLEI GCGC 6 cut(s) 6, 24, 39, 172, 190, 205
AspS9I GGNCC 5 cut(s) 28, 29, 194, 195, 460
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 2 cut(s) 155, 392
BaeGI GKGCMC 2 cut(s) 32, 198
BalI TGGCCA 1 cut(s) 212
BanII GRGCYC 6 cut(s) 32, 131, 198, 297, 335, 897
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 2 cut(s) 335, 897
BbvI GCAGC 6 cut(s) 31, 72, 197, 238, 337, 524
BccI CCATC 4 cut(s) 91, 257, 420, 675
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 1 cut(s) 377
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 2 cut(s) 625, 695
BfaI CTAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 521
BisI GCNGC 6 cut(s) 20, 61, 186, 227, 351, 513
BlsI GCNGC 6 cut(s) 21, 62, 187, 228, 352, 514
Bme1390I CCNGG 2 cut(s) 377, 405
BmgBI CACGTC 1 cut(s) 371
BmgT120I GGNCC 5 cut(s) 28, 29, 194, 195, 460
BmiI GGNNCC 6 cut(s) 30, 31, 130, 196, 197, 296
BmrFI CCNGG 2 cut(s) 377, 405
BmsI GCATC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 1 cut(s) 376
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 56, 205, 222, 418
Bse118I RCCGGY 3 cut(s) 39, 140, 205
Bse1I ACTGG 1 cut(s) 577
BseBI CCWGG 1 cut(s) 377
BseCI ATCGAT 1 cut(s) 858
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 3 cut(s) 571, 918, 935
BseLI CCNNNNNNNGG 5 cut(s) 39, 56, 205, 222, 418
BseMII CTCAG 1 cut(s) 744
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 2 cut(s) 444, 483
BseSI GKGCMC 2 cut(s) 32, 198
BseXI GCAGC 6 cut(s) 31, 72, 197, 238, 337, 524
Bsh1236I CGCG 3 cut(s) 24, 37, 190
BshFI GGCC 8 cut(s) 30, 140, 196, 212, 306, 342, 425, 462
BshVI ATCGAT 1 cut(s) 858
BsiHKAI GWGCWC 2 cut(s) 335, 897
BsiSI CCGG 6 cut(s) 40, 141, 206, 339, 405, 426
BslI CCNNNNNNNGG 5 cut(s) 39, 56, 205, 222, 418
BsmAI GTCTC 2 cut(s) 625, 695
BsnI GGCC 8 cut(s) 30, 140, 196, 212, 306, 342, 425, 462
Bso31I GGTCTC 1 cut(s) 625
Bsp120I GGGCCC 2 cut(s) 28, 194
Bsp1286I GDGCHC 6 cut(s) 32, 131, 198, 297, 335, 897
Bsp143I GATC 2 cut(s) 477, 777
BspACI CCGC 2 cut(s) 501, 694
BspANI GGCC 8 cut(s) 30, 140, 196, 212, 306, 342, 425, 462
BspCNI CTCAG 1 cut(s) 743
BspDI ATCGAT 1 cut(s) 858
BspFNI CGCG 3 cut(s) 24, 37, 190
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 6 cut(s) 30, 31, 130, 196, 197, 296
BspPI GGATC 1 cut(s) 485
BspQI GCTCTTC 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 625
BsrFI RCCGGY 3 cut(s) 39, 140, 205
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 3 cut(s) 39, 140, 205
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 2 cut(s) 477, 777
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 2 cut(s) 15, 181
Bst6I CTCTTC 4 cut(s) 340, 378, 460, 483
BstC8I GCNNGC 2 cut(s) 391, 395
BstDEI CTNAG 3 cut(s) 517, 730, 843
BstF5I GGATG 3 cut(s) 571, 918, 935
BstFNI CGCG 3 cut(s) 24, 37, 190
BstH2I RGCGCY 1 cut(s) 206
BstHHI GCGC 6 cut(s) 6, 24, 39, 172, 190, 205
BstKTI GATC 2 cut(s) 480, 780
BstMAI GTCTC 2 cut(s) 625, 695
BstMBI GATC 2 cut(s) 477, 777
BstMWI GCNNNNNNNGC 8 cut(s) 36, 43, 202, 209, 339, 509, 521, 530
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 375, 403
BstSLI GKGCMC 2 cut(s) 32, 198
BstUI CGCG 3 cut(s) 24, 37, 190
BstV1I GCAGC 6 cut(s) 31, 72, 197, 238, 337, 524
BstV2I GAAGAC 1 cut(s) 606
Bsu15I ATCGAT 1 cut(s) 858
BsuRI GGCC 8 cut(s) 30, 140, 196, 212, 306, 342, 425, 462
BsuTUI ATCGAT 1 cut(s) 858
BtgZI GCGATG 1 cut(s) 803
BtrI CACGTC 1 cut(s) 371
BtsCI GGATG 3 cut(s) 571, 918, 935
BtsIMutI CAGTG 2 cut(s) 20, 186
Cac8I GCNNGC 2 cut(s) 391, 395
CciI TCATGA 1 cut(s) 889
CfoI GCGC 6 cut(s) 6, 24, 39, 172, 190, 205
Cfr10I RCCGGY 3 cut(s) 39, 140, 205
Cfr13I GGNCC 5 cut(s) 28, 29, 194, 195, 460
ClaI ATCGAT 1 cut(s) 858
Csp6I GTAC 1 cut(s) 580
CviAII CATG 1 cut(s) 890
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 517, 730, 843
DpnI GATC 2 cut(s) 479, 779
DpnII GATC 2 cut(s) 477, 777
EaeI YGGCCR 5 cut(s) 138, 210, 304, 340, 423
Eam1104I CTCTTC 4 cut(s) 340, 378, 460, 483
EarI CTCTTC 4 cut(s) 340, 378, 460, 483
Ecl136II GAGCTC 2 cut(s) 333, 895
Eco24I GRGCYC 6 cut(s) 32, 131, 198, 297, 335, 897
Eco31I GGTCTC 1 cut(s) 625
Eco53kI GAGCTC 2 cut(s) 333, 895
Eco57I CTGAAG 1 cut(s) 852
EcoICRI GAGCTC 2 cut(s) 333, 895
EcoO109I RGGNCCY 2 cut(s) 29, 195
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 1 cut(s) 375
EcoT38I GRGCYC 6 cut(s) 32, 131, 198, 297, 335, 897
FaeI CATG 1 cut(s) 893
FaiI YATR 9 cut(s) 561, 563, 687, 784, 786, 881, 891, 899, 910
FatI CATG 1 cut(s) 889
FauNDI CATATG 2 cut(s) 561, 784
Fnu4HI GCNGC 6 cut(s) 20, 61, 186, 227, 351, 513
FokI GGATG 3 cut(s) 578, 922, 925
FriOI GRGCYC 6 cut(s) 32, 131, 198, 297, 335, 897
Fsp4HI GCNGC 6 cut(s) 20, 61, 186, 227, 351, 513
FspBI CTAG 1 cut(s) 671
GlaI GCGC 6 cut(s) 5, 23, 38, 171, 189, 204
GluI GCNGC 6 cut(s) 20, 61, 186, 227, 351, 513
GsuI CTGGAG 1 cut(s) 560
HaeII RGCGCY 1 cut(s) 206
HaeIII GGCC 8 cut(s) 30, 140, 196, 212, 306, 342, 425, 462
HapII CCGG 6 cut(s) 40, 141, 206, 339, 405, 426
HhaI GCGC 6 cut(s) 6, 24, 39, 172, 190, 205
Hin1II CATG 1 cut(s) 893
Hin6I GCGC 6 cut(s) 4, 22, 37, 170, 188, 203
HinP1I GCGC 6 cut(s) 4, 22, 37, 170, 188, 203
HinfI GANTC 4 cut(s) 408, 553, 653, 876
HpaII CCGG 6 cut(s) 40, 141, 206, 339, 405, 426
HphI GGTGA 2 cut(s) 155, 392
Hpy188I TCNGA 3 cut(s) 444, 733, 871
Hpy188III TCNNGA 4 cut(s) 155, 665, 719, 890
HpyAV CCTTC 4 cut(s) 118, 284, 323, 603
HpyCH4III ACNGT 2 cut(s) 15, 181
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 2 cut(s) 353, 830
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 43, 202, 209, 339, 509, 521, 530
HpyF3I CTNAG 3 cut(s) 517, 730, 843
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 1 cut(s) 893
HspAI GCGC 6 cut(s) 4, 22, 37, 170, 188, 203
Kzo9I GATC 2 cut(s) 477, 777
LguI GCTCTTC 1 cut(s) 340
LmnI GCTCC 4 cut(s) 134, 300, 579, 904
Lsp1109I GCAGC 6 cut(s) 31, 72, 197, 238, 337, 524
LweI GCATC 1 cut(s) 390
MaeI CTAG 1 cut(s) 671
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 4 cut(s) 308, 371, 650, 862
MalI GATC 2 cut(s) 479, 779
MboI GATC 2 cut(s) 477, 777
MboII GAAGA 9 cut(s) 327, 395, 447, 463, 477, 500, 608, 611, 641
MhlI GDGCHC 6 cut(s) 32, 131, 198, 297, 335, 897
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 9 cut(s) 52, 112, 133, 147, 218, 278, 299, 455, 761
MluNI TGGCCA 1 cut(s) 212
MlyI GAGTC 2 cut(s) 547, 647
MnlI CCTC 6 cut(s) 379, 422, 425, 438, 461, 514
Mox20I TGGCCA 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 150, 738
MscI TGGCCA 1 cut(s) 212
MseI TTAA 2 cut(s) 747, 755
MslI CAYNNNNRTG 3 cut(s) 369, 564, 940
Msp20I TGGCCA 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 6 cut(s) 40, 141, 206, 339, 405, 426
MspR9I CCNGG 2 cut(s) 377, 405
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 3 cut(s) 24, 37, 190
MwoI GCNNNNNNNGC 8 cut(s) 36, 43, 202, 209, 339, 509, 521, 530
NciI CCSGG 1 cut(s) 405
NdeI CATATG 2 cut(s) 561, 784
NdeII GATC 2 cut(s) 477, 777
NlaIII CATG 1 cut(s) 893
NlaIV GGNNCC 6 cut(s) 30, 31, 130, 196, 197, 296
NmuCI GTSAC 2 cut(s) 371, 650
OliI CACNNNNGTG 1 cut(s) 369
PagI TCATGA 1 cut(s) 889
PciSI GCTCTTC 1 cut(s) 340
PdmI GAANNNNTTC 2 cut(s) 150, 738
PfeI GAWTC 2 cut(s) 408, 876
PflMI CCANNNNNTGG 2 cut(s) 56, 222
PkrI GCNGC 6 cut(s) 21, 62, 187, 228, 352, 514
PleI GAGTC 2 cut(s) 547, 647
PpsI GAGTC 2 cut(s) 547, 647
Psp124BI GAGCTC 2 cut(s) 335, 897
Psp6I CCWGG 1 cut(s) 375
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 6 cut(s) 30, 31, 130, 196, 197, 296
PspOMI GGGCCC 2 cut(s) 28, 194
PspPI GGNCC 5 cut(s) 28, 29, 194, 195, 460
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 3 cut(s) 369, 564, 940
SacI GAGCTC 2 cut(s) 335, 897
SapI GCTCTTC 1 cut(s) 340
SaqAI TTAA 2 cut(s) 747, 755
SatI GCNGC 6 cut(s) 20, 61, 186, 227, 351, 513
Sau3AI GATC 2 cut(s) 477, 777
Sau96I GGNCC 5 cut(s) 28, 29, 194, 195, 460
SchI GAGTC 2 cut(s) 547, 647
ScrFI CCNGG 2 cut(s) 377, 405
SduI GDGCHC 6 cut(s) 32, 131, 198, 297, 335, 897
SfaNI GCATC 1 cut(s) 390
SgrAI CRCCGGYG 1 cut(s) 205
SmiMI CAYNNNNRTG 3 cut(s) 369, 564, 940
Sse9I AATT 9 cut(s) 52, 112, 133, 147, 218, 278, 299, 455, 761
SsiI CCGC 2 cut(s) 501, 694
SspMI CTAG 1 cut(s) 671
SstI GAGCTC 2 cut(s) 335, 897
StyD4I CCNGG 2 cut(s) 375, 403
TaaI ACNGT 2 cut(s) 15, 181
TaiI ACGT 1 cut(s) 373
TaqI TCGA 2 cut(s) 329, 858
TasI AATT 9 cut(s) 52, 112, 133, 147, 218, 278, 299, 455, 761
TfiI GAWTC 2 cut(s) 408, 876
Tru1I TTAA 2 cut(s) 747, 755
Tru9I TTAA 2 cut(s) 747, 755
TscAI CASTG 2 cut(s) 20, 186
TseFI GTSAC 2 cut(s) 371, 650
TseI GCWGC 6 cut(s) 19, 60, 185, 226, 350, 512
Tsp45I GTSAC 2 cut(s) 371, 650
TspDTI ATGAA 2 cut(s) 609, 878
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 2 cut(s) 20, 186
Van91I CCANNNNNTGG 2 cut(s) 56, 222
XapI RAATTY 4 cut(s) 52, 147, 218, 761
XmnI GAANNNNTTC 2 cut(s) 150, 738
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.