Rmu_sc0001966.1_g000019

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001966.1
N/A
Physical Location & Seq
Forward (+)
95455 .. 98300
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
atgggccgagccggcccgtttgccacctctactccttggcaacccaccaccttgctaggcaacgaccacgacatgtatcatggtaggcttcatcatttaacccgtggatcaagtaggtcgatggaggcccccaagcccgacaggaatttaaaactcgggccgacctgccaaagactcttaaaagccagcccagcccgacccgtaagcctacaaactaaatattatactcagtgttctaaaaaacagccgcccaagcagcctaggtgcctcctgggagctgggcggcagcagatcgaggcgagtaaaggctgggcgaatggattgggcattgggctctcaggcagccgcctaggcggtcttggcggaggctgggcgtcttaccaacaccttctctcgaagtcagcgacgatggaggaagacttcaattgcctccaaaaccagatagacaaggcaacggctgacaacggtgagttgaccagagtcatcaaagaagtttctagtgagaggaattctattcgcgatgagtttcgcaagctcaaagctatttcgagagagaaggaagacaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.89

Weight (kDa)

9.81

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 264
AccII CGCG 1 cut(s) 519
AciI CCGC 5 cut(s) 248, 283, 346, 354, 363
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 2 cut(s) 145, 508
AcyI GRCGYC 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 3 cut(s) 278, 535, 542
AluI AGCT 3 cut(s) 278, 535, 542
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 4 cut(s) 4, 13, 126, 158
ApeKI GCWGC 3 cut(s) 256, 286, 342
ApoI RAATTY 2 cut(s) 145, 508
AspA2I CCTAGG 2 cut(s) 260, 349
AspS9I GGNCC 4 cut(s) 4, 14, 127, 158
AsuHPI GGTGA 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 155
AvrII CCTAGG 2 cut(s) 260, 349
BanI GGYRCC 1 cut(s) 264
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 268, 298, 354
BccI CCATC 2 cut(s) 115, 403
BceAI ACGGC 1 cut(s) 471
BciT130I CCWGG 1 cut(s) 272
BfaI CTAG 4 cut(s) 56, 261, 350, 498
BfuAI ACCTGC 1 cut(s) 173
BglI GCCNNNNNGGC 2 cut(s) 12, 351
BisI GCNGC 6 cut(s) 248, 257, 284, 287, 343, 346
BlnI CCTAGG 2 cut(s) 260, 349
BlsI GCNGC 6 cut(s) 249, 258, 285, 288, 344, 347
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 4 cut(s) 4, 14, 127, 158
BmiI GGNNCC 2 cut(s) 129, 266
BmrFI CCNGG 1 cut(s) 272
BoxI GACNNNNGTC 1 cut(s) 479
BpiI GAAGAC 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 35, 103, 260, 271, 349
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bse118I RCCGGY 1 cut(s) 11
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 5 cut(s) 35, 103, 260, 271, 349
BseMII CTCAG 2 cut(s) 242, 351
BseXI GCAGC 3 cut(s) 268, 298, 354
BseYI CCCAGC 4 cut(s) 190, 278, 309, 369
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 4 cut(s) 6, 15, 128, 160
BshNI GGYRCC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 12
BsnI GGCC 4 cut(s) 6, 15, 128, 160
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 2 cut(s) 107, 291
Bsp68I TCGCGA 1 cut(s) 519
BspACI CCGC 5 cut(s) 248, 283, 346, 354, 363
BspANI GGCC 4 cut(s) 6, 15, 128, 160
BspCNI CTCAG 2 cut(s) 241, 350
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 2 cut(s) 129, 266
BspMI ACCTGC 1 cut(s) 173
BspPI GGATC 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 5 cut(s) 35, 103, 260, 271, 349
BssMI GATC 2 cut(s) 107, 291
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 3 cut(s) 35, 260, 349
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 467
BstACI GRCGYC 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 13, 187, 533
BstDEI CTNAG 2 cut(s) 228, 337
BstDSI CCRYGG 1 cut(s) 103
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 2 cut(s) 110, 294
BstMBI GATC 2 cut(s) 107, 291
BstMWI GCNNNNNNNGC 6 cut(s) 12, 191, 253, 256, 351, 360
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 76
BstPAI GACNNNNGTC 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 3 cut(s) 268, 298, 354
BstV2I GAAGAC 1 cut(s) 423
BsuRI GGCC 4 cut(s) 6, 15, 128, 160
BtgI CCRYGG 1 cut(s) 103
BtgZI GCGATG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 236
BtuMI TCGCGA 1 cut(s) 519
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 3 cut(s) 13, 187, 533
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 4, 14, 127, 158
CseI GACGC 1 cut(s) 363
CviAII CATG 2 cut(s) 73, 80
DdeI CTNAG 2 cut(s) 228, 337
DpnI GATC 2 cut(s) 109, 293
DpnII GATC 2 cut(s) 107, 291
DraI TTTAAA 1 cut(s) 150
EciI GGCGGA 1 cut(s) 378
Eco130I CCWWGG 3 cut(s) 35, 260, 349
Eco24I GRGCYC 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 3 cut(s) 35, 260, 349
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 3 cut(s) 35, 260, 349
FaeI CATG 2 cut(s) 76, 83
FaiI YATR 3 cut(s) 74, 81, 225
FatI CATG 2 cut(s) 72, 79
Fnu4HI GCNGC 6 cut(s) 248, 257, 284, 287, 343, 346
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 6 cut(s) 248, 257, 284, 287, 343, 346
FspBI CTAG 4 cut(s) 56, 261, 350, 498
GluI GCNGC 6 cut(s) 248, 257, 284, 287, 343, 346
GsaI CCCAGC 4 cut(s) 194, 282, 313, 373
HaeIII GGCC 4 cut(s) 6, 15, 128, 160
HapII CCGG 1 cut(s) 12
HgaI GACGC 1 cut(s) 363
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 2 cut(s) 76, 83
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 2 cut(s) 174, 480
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 1 cut(s) 474
Hpy188III TCNNGA 3 cut(s) 394, 518, 549
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 398, 550
HpyCH4III ACNGT 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 191, 253, 256, 351, 360
HpyF3I CTNAG 2 cut(s) 228, 337
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 2 cut(s) 76, 83
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 2 cut(s) 107, 291
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 3 cut(s) 268, 298, 354
MaeI CTAG 4 cut(s) 56, 261, 350, 498
MalI GATC 2 cut(s) 109, 293
MboI GATC 2 cut(s) 107, 291
MboII GAAGA 1 cut(s) 428
MfeI CAATTG 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 3 cut(s) 145, 424, 508
MlyI GAGTC 2 cut(s) 168, 489
MnlI CCTC 8 cut(s) 37, 118, 278, 289, 359, 406, 440, 498
MroNI GCCGGC 1 cut(s) 11
MseI TTAA 3 cut(s) 98, 149, 179
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 6 cut(s) 12, 191, 253, 256, 351, 360
NaeI GCCGGC 1 cut(s) 13
NdeII GATC 2 cut(s) 107, 291
NgoMIV GCCGGC 1 cut(s) 11
NlaIII CATG 2 cut(s) 76, 83
NlaIV GGNNCC 2 cut(s) 129, 266
NmeAIII GCCGAG 1 cut(s) 32
NruI TCGCGA 1 cut(s) 519
NspI RCATGY 1 cut(s) 76
PciI ACATGT 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 13
PkrI GCNGC 6 cut(s) 249, 258, 285, 288, 344, 347
PleI GAGTC 2 cut(s) 168, 488
PpsI GAGTC 2 cut(s) 168, 488
PscI ACATGT 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 4 cut(s) 190, 278, 309, 369
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 129, 266
PspPI GGNCC 4 cut(s) 4, 14, 127, 158
RruI TCGCGA 1 cut(s) 519
SaqAI TTAA 3 cut(s) 98, 149, 179
SatI GCNGC 6 cut(s) 248, 257, 284, 287, 343, 346
Sau3AI GATC 2 cut(s) 107, 291
Sau96I GGNCC 4 cut(s) 4, 14, 127, 158
SchI GAGTC 2 cut(s) 168, 489
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 336
SetI ASST 9 cut(s) 29, 53, 119, 167, 266, 280, 390, 537, 544
SfiI GGCCNNNNNGGCC 1 cut(s) 12
Sse9I AATT 3 cut(s) 145, 424, 508
SsiI CCGC 5 cut(s) 248, 283, 346, 354, 363
SspI AATATT 1 cut(s) 221
SspMI CTAG 4 cut(s) 56, 261, 350, 498
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 3 cut(s) 35, 260, 349
TaaI ACNGT 1 cut(s) 467
TaqI TCGA 4 cut(s) 119, 294, 395, 548
TasI AATT 3 cut(s) 145, 424, 508
TauI GCSGC 3 cut(s) 250, 286, 348
Tru1I TTAA 3 cut(s) 98, 149, 179
Tru9I TTAA 3 cut(s) 98, 149, 179
TscAI CASTG 1 cut(s) 236
TseI GCWGC 3 cut(s) 256, 286, 342
TspDTI ATGAA 1 cut(s) 80
TspRI CASTG 1 cut(s) 236
XapI RAATTY 2 cut(s) 145, 508
XceI RCATGY 1 cut(s) 76
XmaJI CCTAGG 2 cut(s) 260, 349
XspI CTAG 4 cut(s) 56, 261, 350, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.