Rmu_sc0001993.1_g000001

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001993.1
N/A
Physical Location & Seq
Reverse (-)
2 .. 792
791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 437 bp
atggattcggggcggcaggttttcacggtggaccttctagaaaggtacgccgcgaagggacgcggagtcatcacgtgtatggcggccggaaacgacgtcattgtgctcggaactagtaaaggatggataatccggcacgattttggggtcggcgattcgttcgatattgatctctctgcgggtcgtcccggggagcattcaattcatagagtttttgttgatcctggagggagccactgtatcgccaacattgctggtaccgggggtgccgatactttctatactcatgcgaaatggagcaagccgcgtcctttggccaagttgaaaggtcttgttgtgaatgctgttgcctggaatagacagcagataacagaagtttcaacaaaggaagttattctcggtacagacaatggccaactttatgagattgctgtgga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

145

Amino Acids

15.58

Weight (kDa)

6.96

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep3_Vps18 PF05131 14 - 144 1.6e-32 Pep3/Vps18/deep orange beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 7
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 257, 266
AccII CGCG 3 cut(s) 53, 63, 307
AciI CCGC 6 cut(s) 13, 51, 63, 83, 179, 305
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 3 cut(s) 84, 315, 412
AcvI CACGTG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 3 cut(s) 47, 259, 403
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 3 cut(s) 201, 325, 381
AhdI GACNNNNNGTC 1 cut(s) 65
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 2 cut(s) 223, 350
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 84, 315, 412
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 3 cut(s) 189, 190, 262
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 31
BalI TGGCCA 2 cut(s) 317, 414
BanI GGYRCC 2 cut(s) 257, 266
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 108
BccI CCATC 1 cut(s) 117
BciT130I CCWGG 2 cut(s) 225, 352
BcnI CCSGG 3 cut(s) 189, 190, 262
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 2 cut(s) 38, 114
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 4 cut(s) 14, 51, 84, 305
BlsI GCNGC 4 cut(s) 15, 52, 85, 306
Bme1390I CCNGG 5 cut(s) 189, 190, 225, 262, 352
Bme18I GGWCC 1 cut(s) 31
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 3 cut(s) 233, 259, 268
BmrFI CCNGG 5 cut(s) 189, 190, 225, 262, 352
BpmI CTGGAG 1 cut(s) 246
BpuMI CCSGG 3 cut(s) 189, 190, 262
BsaAI YACGTR 1 cut(s) 75
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 188, 189, 261
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bse3DI GCAATG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 2 cut(s) 225, 352
BseDI CCNNGG 3 cut(s) 188, 189, 261
BseGI GGATG 1 cut(s) 128
BseJI GATNNNNATC 1 cut(s) 168
BseMI GCAATG 1 cut(s) 249
BseX3I CGGCCG 1 cut(s) 84
Bsh1236I CGCG 3 cut(s) 53, 63, 307
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 3 cut(s) 86, 317, 414
BshNI GGYRCC 2 cut(s) 257, 266
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 87, 133, 189, 261
BslFI GGGAC 2 cut(s) 72, 171
BsmFI GGGAC 2 cut(s) 72, 171
BsmI GAATGC 2 cut(s) 196, 346
BsnI GGCC 3 cut(s) 86, 317, 414
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 2 cut(s) 169, 220
BspACI CCGC 6 cut(s) 13, 51, 63, 83, 179, 305
BspANI GGCC 3 cut(s) 86, 317, 414
BspFNI CGCG 3 cut(s) 53, 63, 307
BspLI GGNNCC 3 cut(s) 233, 259, 268
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 1 cut(s) 215
BspT107I GGYRCC 2 cut(s) 257, 266
BsrDI GCAATG 1 cut(s) 249
BssECI CCNNGG 3 cut(s) 188, 189, 261
BssMI GATC 2 cut(s) 169, 220
BssNI GRCGYC 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 225, 352
Bst4CI ACNGT 2 cut(s) 28, 239
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 302
BstF5I GGATG 1 cut(s) 128
BstFNI CGCG 3 cut(s) 53, 63, 307
BstKTI GATC 2 cut(s) 172, 223
BstMBI GATC 2 cut(s) 169, 220
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 2 cut(s) 225, 352
BstSCI CCNGG 5 cut(s) 187, 188, 223, 260, 350
BstUI CGCG 3 cut(s) 53, 63, 307
BstZI CGGCCG 1 cut(s) 84
BsuRI GGCC 3 cut(s) 86, 317, 414
BtsCI GGATG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 235
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 31
Cfr9I CCCGGG 1 cut(s) 188
CseI GACGC 2 cut(s) 69, 296
Csp6I GTAC 3 cut(s) 46, 258, 402
CviAII CATG 1 cut(s) 287
CviJI RGCY 5 cut(s) 86, 234, 304, 317, 414
CviKI_1 RGCY 5 cut(s) 86, 234, 304, 317, 414
CviQI GTAC 3 cut(s) 46, 258, 402
DpnI GATC 2 cut(s) 171, 222
DpnII GATC 2 cut(s) 169, 220
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 3 cut(s) 84, 315, 412
EagI CGGCCG 1 cut(s) 84
Eam1105I GACNNNNNGTC 1 cut(s) 65
EclXI CGGCCG 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 31
Eco52I CGGCCG 1 cut(s) 84
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 223, 350
FaeI CATG 1 cut(s) 290
FaiI YATR 5 cut(s) 80, 207, 282, 288, 423
FaqI GGGAC 2 cut(s) 72, 171
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 4 cut(s) 14, 51, 84, 305
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 14, 51, 84, 305
FspBI CTAG 2 cut(s) 38, 114
GluI GCNGC 4 cut(s) 14, 51, 84, 305
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 3 cut(s) 86, 317, 414
HapII CCGG 4 cut(s) 87, 133, 189, 261
HgaI GACGC 2 cut(s) 69, 296
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 290
HinfI GANTC 3 cut(s) 5, 66, 155
HpaII CCGG 4 cut(s) 87, 133, 189, 261
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 44, 49
HpyCH4III ACNGT 2 cut(s) 28, 239
HpyCH4IV ACGT 2 cut(s) 74, 96
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpySE526I ACGT 2 cut(s) 74, 96
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 290
KpnI GGTACC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 169, 220
LmnI GCTCC 3 cut(s) 193, 231, 297
MaeI CTAG 2 cut(s) 38, 114
MaeII ACGT 2 cut(s) 74, 96
MalI GATC 2 cut(s) 171, 222
MboI GATC 2 cut(s) 169, 220
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 2 cut(s) 317, 414
MluCI AATT 1 cut(s) 201
MluNI TGGCCA 2 cut(s) 317, 414
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 1 cut(s) 221
Mox20I TGGCCA 2 cut(s) 317, 414
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 2 cut(s) 317, 414
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 2 cut(s) 317, 414
MspI CCGG 4 cut(s) 87, 133, 189, 261
MspR9I CCNGG 5 cut(s) 189, 190, 225, 262, 352
Mva1269I GAATGC 2 cut(s) 196, 346
MvaI CCWGG 2 cut(s) 225, 352
MvnI CGCG 3 cut(s) 53, 63, 307
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 3 cut(s) 189, 190, 262
NdeII GATC 2 cut(s) 169, 220
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 3 cut(s) 233, 259, 268
PctI GAATGC 2 cut(s) 196, 346
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 5, 155
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 4 cut(s) 15, 52, 85, 306
PleI GAGTC 1 cut(s) 74
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 223, 350
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 223, 350
PspN4I GGNNCC 3 cut(s) 233, 259, 268
PspPI GGNCC 1 cut(s) 31
RsaI GTAC 3 cut(s) 47, 259, 403
RsaNI GTAC 3 cut(s) 46, 258, 402
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 4 cut(s) 14, 51, 84, 305
Sau3AI GATC 2 cut(s) 169, 220
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 5 cut(s) 189, 190, 225, 262, 352
SduI GDGCHC 1 cut(s) 108
SetI ASST 6 cut(s) 21, 36, 47, 77, 99, 331
SinI GGWCC 1 cut(s) 31
SmaI CCCGGG 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 77
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 1 cut(s) 201
SsiI CCGC 6 cut(s) 13, 51, 63, 83, 179, 305
SspMI CTAG 2 cut(s) 38, 114
StyD4I CCNGG 5 cut(s) 187, 188, 223, 260, 350
TaaI ACNGT 2 cut(s) 28, 239
TaiI ACGT 2 cut(s) 77, 99
TaqI TCGA 1 cut(s) 162
TasI AATT 1 cut(s) 201
TauI GCSGC 4 cut(s) 16, 53, 86, 307
TfiI GAWTC 2 cut(s) 5, 155
TscAI CASTG 1 cut(s) 242
TspDTI ATGAA 1 cut(s) 194
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 31
XbaI TCTAGA 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 2 cut(s) 38, 114
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.