Rmu_sc0002011.1_g000021

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002011.1
OG0030048
Physical Location & Seq
Forward (+)
76330 .. 77896
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
atgctcttggtgggtgccatcttcaactgcctcgatcctgttttgactattgtttccagtcttagtgtcagagatcctttcttaacaccatttgacaaggaagatcttgcagaggctgctaaatctcagttttcccgagaccacagtgatcaccttgcacttgtgcgtgcctatgagggctggaaggtagctgaaagagactttgctggatatgactactgctggaaaatttttctttcagcacaatcaatgaaggctattgattctctcaggaaggagttctactctttgaaagctaggatggagcgagctagctttggctcctggcggcagcgtagcggcattgacaggttgcggttgaccttcctgaggcatcggagatcaggtcccaatttggatcggatctggtgctttcggggtgcgctaatgcctctcagcggtggcaacgtgttgttggcggggttccttgaagctgggcagcgacgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

162

Amino Acids

18.55

Weight (kDa)

9.66

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HA2_C PF21010 1 - 54 2.2e-13 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0030048)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0399231
rosa_multiflora Rmu_sc0002011.1_g000021

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 5 cut(s) 328, 339, 355, 438, 458
AclWI GGATC 4 cut(s) 29, 68, 405, 410
AcsI RAATTY 1 cut(s) 228
AfiI CCNNNNNNNGG 2 cut(s) 369, 437
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 3 cut(s) 25, 292, 470
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 5 cut(s) 191, 296, 311, 315, 473
AluI AGCT 5 cut(s) 191, 296, 311, 315, 473
Alw26I GTCTC 2 cut(s) 132, 192
AlwI GGATC 4 cut(s) 29, 68, 405, 410
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 116, 331, 478
ApoI RAATTY 1 cut(s) 228
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 143
AsuNHI GCTAGC 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 368
BanI GGYRCC 1 cut(s) 14
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BbvI GCAGC 2 cut(s) 103, 343
BccI CCATC 2 cut(s) 26, 295
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 132, 192
BfaI CTAG 2 cut(s) 297, 312
BglII AGATCT 1 cut(s) 103
BisI GCNGC 5 cut(s) 117, 329, 332, 340, 479
BlsI GCNGC 5 cut(s) 118, 330, 333, 341, 480
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 4 cut(s) 16, 322, 388, 464
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 382
BmtI GCTAGC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 269, 301
BsaI GGTCTC 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 437
Bse1I ACTGG 1 cut(s) 57
Bse21I CCTNAGG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 325
BseGI GGATG 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 369, 437
BseMII CTCAG 4 cut(s) 140, 283, 359, 448
BseNI ACTGG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 103, 343
BseYI CCCAGC 1 cut(s) 473
BshNI GGYRCC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 2 cut(s) 369, 437
BsmAI GTCTC 2 cut(s) 132, 192
BsmFI GGGAC 1 cut(s) 372
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 135
Bsp143I GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
BspACI CCGC 5 cut(s) 328, 339, 355, 438, 458
BspCNI CTCAG 4 cut(s) 139, 282, 360, 447
BspLI GGNNCC 4 cut(s) 16, 322, 388, 464
BspOI GCTAGC 1 cut(s) 315
BspPI GGATC 4 cut(s) 29, 68, 405, 410
BspT107I GGYRCC 1 cut(s) 14
BspTNI GGTCTC 1 cut(s) 132
BsrI ACTGG 1 cut(s) 57
BssMI GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 168, 309, 313
BstDEI CTNAG 5 cut(s) 62, 126, 269, 368, 434
BstENI CCTNNNNNAGG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 7 cut(s) 37, 76, 106, 151, 383, 400, 405
BstMAI GTCTC 2 cut(s) 132, 192
BstMBI GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BstV1I GCAGC 2 cut(s) 103, 343
BstX2I RGATCY 3 cut(s) 73, 103, 402
BstYI RGATCY 3 cut(s) 73, 103, 402
Bsu36I CCTNAGG 1 cut(s) 368
BtsCI GGATG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 3 cut(s) 168, 309, 313
CaiI CAGNNNCTG 1 cut(s) 116
CfoI GCGC 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 386
CviJI RGCY 9 cut(s) 116, 180, 191, 257, 296, 311, 315, 321, 473
CviKI_1 RGCY 9 cut(s) 116, 180, 191, 257, 296, 311, 315, 321, 473
DdeI CTNAG 5 cut(s) 62, 126, 269, 368, 434
DpnI GATC 7 cut(s) 36, 75, 105, 150, 382, 399, 404
DpnII GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 386
Eco81I CCTNAGG 1 cut(s) 368
Eco88I CYCGRG 1 cut(s) 135
EcoNI CCTNNNNNAGG 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 323
FaiI YATR 2 cut(s) 174, 213
FaqI GGGAC 1 cut(s) 372
FauI CCCGC 1 cut(s) 451
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 5 cut(s) 117, 329, 332, 340, 479
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 117, 329, 332, 340, 479
FspBI CTAG 2 cut(s) 297, 312
GlaI GCGC 1 cut(s) 423
GluI GCNGC 5 cut(s) 117, 329, 332, 340, 479
GsaI CCCAGC 1 cut(s) 477
HhaI GCGC 1 cut(s) 424
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 1 cut(s) 263
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 3 cut(s) 71, 378, 402
Hpy188III TCNNGA 3 cut(s) 135, 271, 367
Hpy8I GTNNAC 1 cut(s) 360
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 4 cut(s) 178, 247, 268, 373
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 2 cut(s) 110, 158
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 5 cut(s) 62, 126, 269, 368, 434
HpySE526I ACGT 1 cut(s) 447
HspAI GCGC 1 cut(s) 422
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
LmnI GCTCC 2 cut(s) 304, 326
Lsp1109I GCAGC 2 cut(s) 103, 343
LweI GCATC 1 cut(s) 382
MaeI CTAG 2 cut(s) 297, 312
MaeII ACGT 1 cut(s) 447
MalI GATC 7 cut(s) 36, 75, 105, 150, 382, 399, 404
MboI GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
MboII GAAGA 2 cut(s) 13, 113
MflI RGATCY 3 cut(s) 73, 103, 402
MluCI AATT 2 cut(s) 228, 391
MnlI CCTC 5 cut(s) 41, 106, 169, 363, 441
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeII GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
NheI GCTAGC 1 cut(s) 311
NlaIV GGNNCC 4 cut(s) 16, 322, 388, 464
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 5 cut(s) 118, 330, 333, 341, 480
PpuMI RGGWCCY 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 4 cut(s) 16, 322, 388, 464
PspPI GGNCC 1 cut(s) 386
PspPPI RGGWCCY 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 3 cut(s) 73, 103, 402
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 5 cut(s) 117, 329, 332, 340, 479
Sau3AI GATC 7 cut(s) 34, 73, 103, 148, 380, 397, 402
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 325
SfaNI GCATC 1 cut(s) 382
SinI GGWCC 1 cut(s) 386
Sse9I AATT 2 cut(s) 228, 391
SsiI CCGC 5 cut(s) 328, 339, 355, 438, 458
SspMI CTAG 2 cut(s) 297, 312
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 1 cut(s) 146
TaiI ACGT 1 cut(s) 450
TaqI TCGA 1 cut(s) 33
TasI AATT 2 cut(s) 228, 391
TauI GCSGC 2 cut(s) 331, 342
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 1 cut(s) 151
TseI GCWGC 3 cut(s) 116, 331, 478
TspDTI ATGAA 1 cut(s) 266
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 386
XagI CCTNNNNNAGG 1 cut(s) 367
XapI RAATTY 1 cut(s) 228
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 2 cut(s) 297, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.