Rmu_sc0002130.1_g000003

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002130.1
N/A
Physical Location & Seq
Forward (+)
17472 .. 19640
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
atggccagagacctcaccggagttggctggggggttcaaaatccctcgggggctttgttcttctgctcctccttgctgcgatctgcgccgtggccggagcatcgccaaccccaaaatccagtaacaaaacaagcatcggagaaggaggaggaggaagcatatacgatcacctccggcaataagggttacccatcggcctcctccccaaaggcatcacggaatattccctcgacaattccagcggcgaattccggccgaatcgccgacttatccggcgtctccgctcaggaactcttcctctggtttccggttaaaggtatccgagtcgatgtgcccagctccggcctcatttacttcgatgtcggcgttgtcgataagcagttctctttgtccctcttcgagtctccgcccgattgcactgctcttgatccttctgatccctcctctccttcttcttcttccaattccccatctgagctcaagctcaagaaggtggaggagatgtggtggtcgctggggtcgagttttatgggtttgggtatgtggagggagggagggaaggagggaggagatgcggtggtcgccagggtcgaactttctgggttcggggtctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

204

Amino Acids

21.8

Weight (kDa)

4.99

Isoelectric Point (pI)

59.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 83 - 134 2.7e-12 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 284
AciI CCGC 4 cut(s) 242, 282, 407, 575
AclWI GGATC 2 cut(s) 422, 431
AcoI YGGCCR 3 cut(s) 3, 92, 253
AcsI RAATTY 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 276
AfiI CCNNNNNNNGG 2 cut(s) 314, 341
AgsI TTSAA 1 cut(s) 38
AjnI CCWGG 1 cut(s) 584
AluBI AGCT 3 cut(s) 339, 478, 484
AluI AGCT 3 cut(s) 339, 478, 484
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 3 cut(s) 3, 283, 408
AlwI GGATC 2 cut(s) 422, 431
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 5 cut(s) 3, 92, 195, 253, 343
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 1 cut(s) 247
AspLEI GCGC 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 7, 160
AvaI CYCGRG 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 336
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 63
BccI CCATC 2 cut(s) 199, 478
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 1 cut(s) 586
BciVI GTATCC 1 cut(s) 329
BcoDI GTCTC 3 cut(s) 3, 283, 408
BfaI CTAG 1 cut(s) 613
BfuI GTATCC 1 cut(s) 329
BisI GCNGC 2 cut(s) 77, 243
BlsI GCNGC 2 cut(s) 78, 244
Bme1390I CCNGG 1 cut(s) 586
BmeT110I CYCGRG 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 586
BmsI GCATC 4 cut(s) 109, 143, 221, 562
Bpu10I CCTNAGC 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 464, 470
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 3 cut(s) 45, 89, 585
BsaWI WCCGGW 2 cut(s) 17, 307
BsaXI ACNNNNNCTCC 6 cut(s) 488, 491, 518, 521, 561, 591
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 341
Bse1I ACTGG 1 cut(s) 119
BseBI CCWGG 1 cut(s) 586
BseDI CCNNGG 3 cut(s) 45, 89, 585
BseLI CCNNNNNNNGG 2 cut(s) 314, 341
BseMII CTCAG 2 cut(s) 299, 465
BseNI ACTGG 1 cut(s) 119
BseRI GAGGAG 7 cut(s) 58, 161, 164, 190, 433, 512, 582
BseSI GKGCMC 1 cut(s) 336
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 3 cut(s) 27, 335, 514
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 5 cut(s) 5, 94, 197, 255, 345
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 7 cut(s) 18, 95, 174, 252, 273, 308, 342
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 2 cut(s) 314, 341
BsmAI GTCTC 3 cut(s) 3, 283, 408
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 376
BsnI GGCC 5 cut(s) 5, 94, 197, 255, 345
Bso31I GGTCTC 1 cut(s) 3
BsoBI CYCGRG 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 336, 480
Bsp143I GATC 4 cut(s) 80, 165, 427, 436
BspACI CCGC 4 cut(s) 242, 282, 407, 575
BspANI GGCC 5 cut(s) 5, 94, 197, 255, 345
BspCNI CTCAG 2 cut(s) 298, 466
BspPI GGATC 2 cut(s) 422, 431
BspTNI GGTCTC 1 cut(s) 3
BsrBI CCGCTC 1 cut(s) 284
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 45, 89, 585
BssMI GATC 4 cut(s) 80, 165, 427, 436
BssNI GRCGYC 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 586
Bst6I CTCTTC 2 cut(s) 299, 401
BstACI GRCGYC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 285, 474
BstDSI CCRYGG 1 cut(s) 89
BstEII GGTNACC 1 cut(s) 185
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 83, 168, 430, 439
BstMAI GTCTC 3 cut(s) 3, 283, 408
BstMBI GATC 4 cut(s) 80, 165, 427, 436
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 2 cut(s) 85, 581
BstNI CCWGG 1 cut(s) 586
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 63
BstZI CGGCCG 1 cut(s) 253
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 5 cut(s) 5, 94, 197, 255, 345
BtgI CCRYGG 1 cut(s) 89
BtgZI GCGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
CfoI GCGC 1 cut(s) 88
CseI GACGC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 285, 474
DpnI GATC 4 cut(s) 82, 167, 429, 438
DpnII GATC 4 cut(s) 80, 165, 427, 436
EaeI YGGCCR 3 cut(s) 3, 92, 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 299, 401
EarI CTCTTC 2 cut(s) 299, 401
EciI GGCGGA 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 478
EclXI CGGCCG 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 3
Eco52I CGGCCG 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 46
Eco91I GGTNACC 1 cut(s) 185
EcoICRI GAGCTC 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 480
Esp3I CGTCTC 1 cut(s) 283
FaiI YATR 4 cut(s) 160, 162, 530, 542
FaqI GGGAC 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 77, 243
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 2 cut(s) 77, 243
FspBI CTAG 1 cut(s) 613
GlaI GCGC 1 cut(s) 87
GluI GCNGC 2 cut(s) 77, 243
GsaI CCCAGC 3 cut(s) 31, 339, 518
HaeIII GGCC 5 cut(s) 5, 94, 197, 255, 345
HapII CCGG 7 cut(s) 18, 95, 174, 252, 273, 308, 342
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 276
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 3 cut(s) 258, 324, 401
HpaII CCGG 7 cut(s) 18, 95, 174, 252, 273, 308, 342
HphI GGTGA 2 cut(s) 7, 160
Hpy188I TCNGA 4 cut(s) 139, 323, 436, 475
Hpy188III TCNNGA 3 cut(s) 287, 425, 487
HpyAV CCTTC 5 cut(s) 136, 441, 459, 484, 553
HpyCH4V TGCA 1 cut(s) 417
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 581
HpyF3I CTNAG 2 cut(s) 285, 474
Hsp92I GRCGYC 1 cut(s) 276
HspAI GCGC 1 cut(s) 86
Kzo9I GATC 4 cut(s) 80, 165, 427, 436
LmnI GCTCC 3 cut(s) 71, 97, 344
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 4 cut(s) 109, 143, 221, 562
MaeI CTAG 1 cut(s) 613
MaeIII GTNAC 2 cut(s) 121, 185
MalI GATC 4 cut(s) 82, 167, 429, 438
MbiI CCGCTC 1 cut(s) 284
MboI GATC 4 cut(s) 80, 165, 427, 436
MboII GAAGA 6 cut(s) 52, 286, 388, 444, 447, 450
MhlI GDGCHC 2 cut(s) 336, 480
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 234, 247, 463
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 333, 410
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 7 cut(s) 18, 95, 174, 252, 273, 308, 342
MspR9I CCNGG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 85, 581
NdeII GATC 4 cut(s) 80, 165, 427, 436
PcsI WCGNNNNNNNCGW 4 cut(s) 363, 369, 518, 588
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 2 cut(s) 78, 244
PleI GAGTC 2 cut(s) 332, 409
PpsI GAGTC 2 cut(s) 332, 409
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 584
PspEI GGTNACC 1 cut(s) 185
PspFI CCCAGC 3 cut(s) 27, 335, 514
PspGI CCWGG 1 cut(s) 584
SacI GAGCTC 1 cut(s) 480
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 2 cut(s) 77, 243
Sau3AI GATC 4 cut(s) 80, 165, 427, 436
SchI GAGTC 2 cut(s) 333, 410
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 2 cut(s) 336, 480
SetI ASST 7 cut(s) 15, 173, 319, 341, 480, 486, 495
SfaNI GCATC 4 cut(s) 109, 143, 221, 562
SmlI CTYRAG 2 cut(s) 479, 485
SmoI CTYRAG 2 cut(s) 479, 485
Sse9I AATT 3 cut(s) 234, 247, 463
SsiI CCGC 4 cut(s) 242, 282, 407, 575
SspI AATATT 1 cut(s) 223
SspMI CTAG 1 cut(s) 613
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 584
TaqI TCGA 7 cut(s) 230, 327, 357, 372, 399, 521, 591
TasI AATT 3 cut(s) 234, 247, 463
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 1 cut(s) 424
TseI GCWGC 1 cut(s) 76
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 1 cut(s) 424
XapI RAATTY 1 cut(s) 247
XspI CTAG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.