Rmu_sc0002161.1_g000006

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002161.1
N/A
Physical Location & Seq
Reverse (-)
28682 .. 30821
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
atggaggcactcagtagcattttcctactaggcctttgccttctctttgcttcatcggcaatgtcgaccttggccgatcccaaagctcaccaccatgaatttgttatccaagcaacaccagtgaagaggctgtgcaagacccacaacaccatcacagtgaatggacagttccctggaccgaccttggaagtaaacaatggcgacactctggtcgtcaaagtcactaacaaagctcgatataatgtcaccattcactggcatggcattcggcaaatgagaaccggatgggcggatgggcctgagtttgttactcaatgtcctattagaccaggtaggagttacacctaccggtttacagtccaaggtcaagaaggtactctctggtggcacgctcatagctcatggcttagagccaccgtgtatggagcacttattattcatccgaaacaaggagactcgtatccattcactaaaccgaatcgtcaagcaacacttcttcttggtgagtggtgggatggtaaccctattgacgtggtgaggagggcgactcgcacaggagcagctccaaatgtttctaacgcatataccattaacgccattcttgaatacaagtccgttcgttgcaacgcaactacttgcaacaagggtaaaacagtaattaaacccataatgccaaagctgccagctttcaatgacacaaacacagcatctgccttcactaagagcttcagaagccctagaaaagtcgaagtcccaactgaaatcgacgagaacctcttcttcacaatcggcctcggactcaacaaatgccccaagcactttcgatctagaaggtgccaaggccccaatggcacacgcttcactgctagcatgaacaatgtctcatttgtgcttccaaacaacatgtcaattctccaagcttaccagcaaaacattcctggagtttacactaatgattttccagccaacccaccacggaagtttgattacactgggaatgtcagccgctcgctttggcaacccagttctggaacaaagggatacaagttgaagtacgggtctagagtgcaggtggtgttacaagacacaagtattgtcacacccgagaaccatcccattcatctccacggttatgatttctacatccttgcagagggttttggaaatttcaaccccaagacggatactaaaatgtttaatcttgttgatccacctctaaggaacacggtcgcggtgcccgtaaatggttgggcagtcatcagatttgtagctgacaatccaggtgcttggatgatgcattgtcacttggatgttcatatcaactggggtttggccatggttttcttggtggacaatggagttggggagctgcaatcaatagagccaccgccagtggattatcctctgtgttcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0010035 GO:0010038 GO:0016491 GO:0016722 GO:0042221 GO:0046688 GO:0050896 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

471

Amino Acids

52.67

Weight (kDa)

9.35

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 36 - 150 1e-45 Multicopper oxidase
Cu-oxidase_2 PF07731 319 - 452 2.5e-43 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1060
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 1060
AccB1I GGYRCC 2 cut(s) 834, 1234
AccB7I CCANNNNNTGG 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 1008
AccI GTMKAC 1 cut(s) 65
AccII CGCG 1 cut(s) 1232
AciI CCGC 4 cut(s) 290, 1006, 1232, 1388
AclWI GGATC 2 cut(s) 71, 1202
AcoI YGGCCR 2 cut(s) 72, 1332
AcsI RAATTY 2 cut(s) 98, 1165
AcuI CTGAAG 1 cut(s) 712
AfaI GTAC 2 cut(s) 376, 1055
AfiI CCNNNNNNNGG 6 cut(s) 255, 449, 1028, 1153, 1180, 1244
AflIII ACRYGT 1 cut(s) 903
AgeI ACCGGT 1 cut(s) 348
AgsI TTSAA 4 cut(s) 605, 691, 1051, 1171
AjiI CACGTC 1 cut(s) 532
AjnI CCWGG 4 cut(s) 172, 328, 937, 1279
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 447, 886
AlwI GGATC 2 cut(s) 71, 1202
AlwNI CAGNNNCTG 1 cut(s) 710
Ama87I CYCGRG 1 cut(s) 1103
AoxI GGCC 6 cut(s) 31, 72, 296, 790, 841, 1332
ApeKI GCWGC 3 cut(s) 560, 679, 1369
ApoI RAATTY 2 cut(s) 98, 1165
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
AsiGI ACCGGT 1 cut(s) 348
Asp700I GAANNNNTTC 1 cut(s) 776
AspS9I GGNCC 3 cut(s) 176, 296, 842
AsuHPI GGTGA 4 cut(s) 80, 238, 515, 547
AsuNHI GCTAGC 1 cut(s) 866
AvaI CYCGRG 1 cut(s) 1103
AvaII GGWCC 1 cut(s) 176
BaeGI GKGCMC 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 1334
BanI GGYRCC 2 cut(s) 834, 1234
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 3 cut(s) 572, 666, 1356
BccI CCATC 5 cut(s) 158, 279, 287, 509, 1119
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 4 cut(s) 174, 330, 939, 1281
BciVI GTATCC 3 cut(s) 471, 1034, 1177
BcoDI GTCTC 2 cut(s) 447, 886
BfaI CTAG 5 cut(s) 29, 738, 828, 867, 1062
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 3 cut(s) 471, 1034, 1177
BglI GCCNNNNNGGC 1 cut(s) 849
BisI GCNGC 4 cut(s) 561, 680, 1006, 1370
BlsI GCNGC 4 cut(s) 562, 681, 1007, 1371
Bme1390I CCNGG 4 cut(s) 174, 330, 939, 1281
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 1103
BmgBI CACGTC 1 cut(s) 532
BmgT120I GGNCC 3 cut(s) 176, 296, 842
BmiI GGNNCC 3 cut(s) 836, 844, 1236
BmrFI CCNGG 4 cut(s) 174, 330, 939, 1281
BmrI ACTGGG 3 cut(s) 1002, 1017, 1333
BmsI GCATC 2 cut(s) 716, 1284
BmtI GCTAGC 1 cut(s) 870
BmuI ACTGGG 3 cut(s) 1002, 1017, 1333
BplI GAGNNNNNCTC 2 cut(s) 532, 564
BpmI CTGGAG 1 cut(s) 960
BsaJI CCNNGG 9 cut(s) 69, 172, 183, 361, 793, 838, 974, 1126, 1335
BsaWI WCCGGW 2 cut(s) 281, 348
Bsc4I CCNNNNNNNGG 6 cut(s) 255, 449, 1028, 1153, 1180, 1244
Bse118I RCCGGY 1 cut(s) 348
Bse1I ACTGG 6 cut(s) 119, 260, 997, 1023, 1328, 1391
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 4 cut(s) 174, 330, 939, 1281
BseDI CCNNGG 9 cut(s) 69, 172, 183, 361, 793, 838, 974, 1126, 1335
BseGI GGATG 8 cut(s) 290, 298, 439, 520, 1111, 1143, 1296, 1315
BseLI CCNNNNNNNGG 6 cut(s) 255, 449, 1028, 1153, 1180, 1244
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 2 cut(s) 25, 291
BseNI ACTGG 6 cut(s) 119, 260, 997, 1023, 1328, 1391
BseRI GAGGAG 1 cut(s) 553
BseSI GKGCMC 1 cut(s) 1239
BseXI GCAGC 3 cut(s) 572, 666, 1356
BsgI GTGCAG 1 cut(s) 1088
Bsh1236I CGCG 1 cut(s) 1232
Bsh1285I CGRYCG 1 cut(s) 1230
BshFI GGCC 6 cut(s) 33, 74, 298, 792, 843, 1334
BshNI GGYRCC 2 cut(s) 834, 1234
BshTI ACCGGT 1 cut(s) 348
BsiEI CGRYCG 1 cut(s) 1230
BsiHKAI GWGCWC 1 cut(s) 430
BsiHKCI CYCGRG 1 cut(s) 1103
BsiSI CCGG 2 cut(s) 282, 349
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 6 cut(s) 255, 449, 1028, 1153, 1180, 1244
BsmAI GTCTC 2 cut(s) 447, 886
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 6 cut(s) 33, 74, 298, 792, 843, 1334
BsoBI CYCGRG 1 cut(s) 1103
Bsp1286I GDGCHC 2 cut(s) 430, 1239
Bsp143I GATC 3 cut(s) 76, 824, 1207
Bsp19I CCATGG 1 cut(s) 1335
BspACI CCGC 4 cut(s) 290, 1006, 1232, 1388
BspANI GGCC 6 cut(s) 33, 74, 298, 792, 843, 1334
BspCNI CTCAG 2 cut(s) 24, 292
BspFNI CGCG 1 cut(s) 1232
BspLI GGNNCC 3 cut(s) 836, 844, 1236
BspMI ACCTGC 1 cut(s) 1060
BspOI GCTAGC 1 cut(s) 870
BspPI GGATC 2 cut(s) 71, 1202
BspT107I GGYRCC 2 cut(s) 834, 1234
BsrBI CCGCTC 1 cut(s) 1008
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 1 cut(s) 348
BsrI ACTGG 6 cut(s) 119, 260, 997, 1023, 1328, 1391
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 9 cut(s) 69, 172, 183, 361, 793, 838, 974, 1126, 1335
BssMI GATC 3 cut(s) 76, 824, 1207
BssT1I CCWWGG 5 cut(s) 69, 183, 361, 838, 1335
Bst2UI CCWGG 4 cut(s) 174, 330, 939, 1281
Bst4CI ACNGT 7 cut(s) 157, 168, 358, 418, 655, 1130, 1228
Bst6I CTCTTC 2 cut(s) 119, 782
BstC8I GCNNGC 4 cut(s) 390, 684, 868, 1010
BstDEI CTNAG 5 cut(s) 11, 300, 407, 720, 1217
BstDSI CCRYGG 3 cut(s) 974, 1126, 1335
BstEII GGTNACC 1 cut(s) 518
BstENI CCTNNNNNAGG 1 cut(s) 1151
BstF5I GGATG 8 cut(s) 290, 298, 439, 520, 1111, 1143, 1296, 1315
BstFNI CGCG 1 cut(s) 1232
BstKTI GATC 3 cut(s) 79, 827, 1210
BstMAI GTCTC 2 cut(s) 447, 886
BstMBI GATC 3 cut(s) 76, 824, 1207
BstMCI CGRYCG 1 cut(s) 1230
BstMWI GCNNNNNNNGC 4 cut(s) 56, 679, 732, 849
BstNI CCWGG 4 cut(s) 174, 330, 939, 1281
BstNSI RCATGY 1 cut(s) 907
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 4 cut(s) 172, 328, 937, 1279
BstSLI GKGCMC 1 cut(s) 1239
BstUI CGCG 1 cut(s) 1232
BstV1I GCAGC 3 cut(s) 572, 666, 1356
BstXI CCANNNNNNTGG 1 cut(s) 1287
BsuI GTATCC 3 cut(s) 471, 1034, 1177
BsuRI GGCC 6 cut(s) 33, 74, 298, 792, 843, 1334
BtgI CCRYGG 3 cut(s) 974, 1126, 1335
BtrI CACGTC 1 cut(s) 532
BtsCI GGATG 8 cut(s) 290, 298, 439, 520, 1111, 1143, 1296, 1315
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 6 cut(s) 126, 162, 253, 861, 990, 1398
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 4 cut(s) 390, 684, 868, 1010
CaiI CAGNNNCTG 1 cut(s) 710
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 3 cut(s) 176, 296, 842
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 2 cut(s) 375, 1054
CspAI ACCGGT 1 cut(s) 348
CviAII CATG 6 cut(s) 95, 260, 402, 871, 904, 1336
CviQI GTAC 2 cut(s) 375, 1054
DdeI CTNAG 5 cut(s) 11, 300, 407, 720, 1217
DpnI GATC 3 cut(s) 78, 826, 1209
DpnII GATC 3 cut(s) 76, 824, 1207
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 2 cut(s) 72, 1332
Eam1104I CTCTTC 2 cut(s) 119, 782
EarI CTCTTC 2 cut(s) 119, 782
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 5 cut(s) 69, 183, 361, 838, 1335
Eco147I AGGCCT 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 712
Eco88I CYCGRG 1 cut(s) 1103
Eco91I GGTNACC 1 cut(s) 518
EcoNI CCTNNNNNAGG 1 cut(s) 1151
EcoO109I RGGNCCY 1 cut(s) 842
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 4 cut(s) 172, 328, 937, 1279
EcoT14I CCWWGG 5 cut(s) 69, 183, 361, 838, 1335
EcoT22I ATGCAT 1 cut(s) 1299
ErhI CCWWGG 5 cut(s) 69, 183, 361, 838, 1335
FaeI CATG 6 cut(s) 98, 263, 405, 874, 907, 1339
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FaqI GGGAC 1 cut(s) 737
FatI CATG 6 cut(s) 94, 259, 401, 870, 903, 1335
FblI GTMKAC 1 cut(s) 65
Fnu4HI GCNGC 4 cut(s) 561, 680, 1006, 1370
FokI GGATG 8 cut(s) 297, 305, 426, 527, 1098, 1130, 1303, 1322
Fsp4HI GCNGC 4 cut(s) 561, 680, 1006, 1370
FspBI CTAG 5 cut(s) 29, 738, 828, 867, 1062
GluI GCNGC 4 cut(s) 561, 680, 1006, 1370
GsuI CTGGAG 1 cut(s) 960
HaeIII GGCC 6 cut(s) 33, 74, 298, 792, 843, 1334
HapII CCGG 2 cut(s) 282, 349
Hin1II CATG 6 cut(s) 98, 263, 405, 874, 907, 1339
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HindIII AAGCTT 1 cut(s) 918
HinfI GANTC 4 cut(s) 455, 478, 547, 798
HpaII CCGG 2 cut(s) 282, 349
HphI GGTGA 4 cut(s) 80, 238, 515, 547
Hpy166II GTNNAC 5 cut(s) 66, 193, 354, 946, 1351
Hpy188I TCNGA 4 cut(s) 444, 731, 797, 1262
Hpy188III TCNNGA 5 cut(s) 368, 602, 828, 1029, 1062
Hpy8I GTNNAC 5 cut(s) 66, 193, 354, 946, 1351
Hpy99I CGWCG 1 cut(s) 770
HpyAV CCTTC 4 cut(s) 50, 365, 724, 825
HpyCH4III ACNGT 7 cut(s) 157, 168, 358, 418, 655, 1130, 1228
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 7 cut(s) 135, 624, 639, 1069, 1151, 1297, 1372
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 679, 732, 849
HpyF3I CTNAG 5 cut(s) 11, 300, 407, 720, 1217
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 6 cut(s) 98, 263, 405, 874, 907, 1339
Kzo9I GATC 3 cut(s) 76, 824, 1207
LmnI GCTCC 4 cut(s) 425, 557, 568, 1366
Lsp1109I GCAGC 3 cut(s) 572, 666, 1356
LweI GCATC 2 cut(s) 716, 1284
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 5 cut(s) 29, 738, 828, 867, 1062
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 8 cut(s) 220, 244, 307, 338, 518, 1077, 1096, 1301
MalI GATC 3 cut(s) 78, 826, 1209
MbiI CCGCTC 1 cut(s) 1008
MboI GATC 3 cut(s) 76, 824, 1207
MboII GAAGA 4 cut(s) 136, 488, 769, 772
MhlI GDGCHC 2 cut(s) 430, 1239
MlsI TGGCCA 1 cut(s) 1334
MluCI AATT 4 cut(s) 98, 657, 909, 1165
MluNI TGGCCA 1 cut(s) 1334
MlyI GAGTC 3 cut(s) 449, 541, 792
MnlI CCTC 8 cut(s) 120, 531, 534, 785, 803, 1147, 1224, 1413
Mox20I TGGCCA 1 cut(s) 1334
Mph1103I ATGCAT 1 cut(s) 1299
MroXI GAANNNNTTC 1 cut(s) 776
MscI TGGCCA 1 cut(s) 1334
MseI TTAA 3 cut(s) 591, 660, 1197
MslI CAYNNNNRTG 5 cut(s) 93, 155, 258, 1131, 1308
Msp20I TGGCCA 1 cut(s) 1334
MspI CCGG 2 cut(s) 282, 349
MspR9I CCNGG 4 cut(s) 174, 330, 939, 1281
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 4 cut(s) 174, 330, 939, 1281
MvnI CGCG 1 cut(s) 1232
MwoI GCNNNNNNNGC 4 cut(s) 56, 679, 732, 849
NcoI CCATGG 1 cut(s) 1335
NdeII GATC 3 cut(s) 76, 824, 1207
NheI GCTAGC 1 cut(s) 866
NlaIII CATG 6 cut(s) 98, 263, 405, 874, 907, 1339
NlaIV GGNNCC 3 cut(s) 836, 844, 1236
NmuCI GTSAC 4 cut(s) 220, 244, 1096, 1301
NsiI ATGCAT 1 cut(s) 1299
NspI RCATGY 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 1060
PceI AGGCCT 1 cut(s) 33
PciI ACATGT 1 cut(s) 903
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 1236
PctI GAATGC 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 776
PfeI GAWTC 1 cut(s) 478
PflMI CCANNNNNTGG 1 cut(s) 255
PfoI TCCNGGA 1 cut(s) 937
PinAI ACCGGT 1 cut(s) 348
PkrI GCNGC 4 cut(s) 562, 681, 1007, 1371
PleI GAGTC 3 cut(s) 449, 541, 792
PpsI GAGTC 3 cut(s) 449, 541, 792
PscI ACATGT 1 cut(s) 903
Psp6I CCWGG 4 cut(s) 172, 328, 937, 1279
PspEI GGTNACC 1 cut(s) 518
PspGI CCWGG 4 cut(s) 172, 328, 937, 1279
PspN4I GGNNCC 3 cut(s) 836, 844, 1236
PspPI GGNCC 3 cut(s) 176, 296, 842
PstNI CAGNNNCTG 1 cut(s) 710
RsaI GTAC 2 cut(s) 376, 1055
RsaNI GTAC 2 cut(s) 375, 1054
RseI CAYNNNNRTG 5 cut(s) 93, 155, 258, 1131, 1308
SalI GTCGAC 1 cut(s) 64
SaqAI TTAA 3 cut(s) 591, 660, 1197
SatI GCNGC 4 cut(s) 561, 680, 1006, 1370
Sau3AI GATC 3 cut(s) 76, 824, 1207
Sau96I GGNCC 3 cut(s) 176, 296, 842
SchI GAGTC 3 cut(s) 449, 541, 792
ScrFI CCNGG 4 cut(s) 174, 330, 939, 1281
SduI GDGCHC 2 cut(s) 430, 1239
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 2 cut(s) 716, 1284
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 5 cut(s) 93, 155, 258, 1131, 1308
Sse9I AATT 4 cut(s) 98, 657, 909, 1165
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 4 cut(s) 290, 1006, 1232, 1388
SspMI CTAG 5 cut(s) 29, 738, 828, 867, 1062
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 4 cut(s) 172, 328, 937, 1279
StyI CCWWGG 5 cut(s) 69, 183, 361, 838, 1335
TaaI ACNGT 7 cut(s) 157, 168, 358, 418, 655, 1130, 1228
TaiI ACGT 1 cut(s) 534
TaqI TCGA 5 cut(s) 65, 235, 747, 765, 823
TaqII GACCGA 1 cut(s) 193
TasI AATT 4 cut(s) 98, 657, 909, 1165
TauI GCSGC 1 cut(s) 1008
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 3 cut(s) 591, 660, 1197
Tru9I TTAA 3 cut(s) 591, 660, 1197
TscAI CASTG 6 cut(s) 126, 162, 260, 868, 997, 1398
TseFI GTSAC 4 cut(s) 220, 244, 1096, 1301
TseI GCWGC 3 cut(s) 560, 679, 1369
Tsp45I GTSAC 4 cut(s) 220, 244, 1096, 1301
TspDTI ATGAA 6 cut(s) 42, 111, 428, 887, 1109, 1304
TspGWI ACGGA 3 cut(s) 604, 991, 1196
TspRI CASTG 6 cut(s) 126, 162, 260, 868, 997, 1398
Van91I CCANNNNNTGG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 1151
XapI RAATTY 2 cut(s) 98, 1165
XbaI TCTAGA 2 cut(s) 827, 1061
XceI RCATGY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 2 cut(s) 845, 1342
XmiI GTMKAC 1 cut(s) 65
XmnI GAANNNNTTC 1 cut(s) 776
XspI CTAG 5 cut(s) 29, 738, 828, 867, 1062
Zsp2I ATGCAT 1 cut(s) 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.