Rmu_sc0002180.1_g000033

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002180.1
N/A
Physical Location & Seq
Forward (+)
117367 .. 119889
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
atgaataacctcatcagctcaagcaaggatatggatttccttcgtgacaaaggaataatggttaggtggttgagctctaaagatgcttcccaatttttcaacaagctttacaatgacacctttctcaagcaattctactatggtggactctgtgctgaagtgaatactgaatatgacgcagtcggaaaagtaaccctaggtttacaagcaataaacttaaaacaagaaatctggagttcaccagagataaatgagaaaactctcaatttgattgataatagggatcttgaccatttacccaattttggcctagtaatttctcacttactctatcgcttgaaagaagtcaagtcaatggacaccctcgatgaccgcacccaatggcctccggctaatcagactgccggtctctcaaagccttcaattgcagaacgatatcaatcagatcagaaactcaggccatcggtgaatcagaccgatgcaatccctttatccctatcactcatcttctatgatgcttctcgtctctcctcctttgatcaattctatttctcttactttctctcagagatcggagcccagccatgtccgacgaggagcaccacttcgagtctaaggccgacgccggagcctccaagacctatcctcaatagaccggcaccatctgaaagaacggattgtgacggattggatagtgaagagagaagagaagaggaggaagaagaagagaatggtgttgtcatggttgttaagatagtgaagagagaagagaagagaggagataaggtgtggtgtggggggcatgacgtgcaccgattgtgggttgggattgatattgactccaccgaaccagaaccagatggcgatggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

33.04

Weight (kDa)

4.84

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 60 2.2e-09 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 658
AciI CCGC 1 cut(s) 373
AclWI GGATC 1 cut(s) 291
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 623
AfiI CCNNNNNNNGG 2 cut(s) 305, 820
AgsI TTSAA 3 cut(s) 100, 340, 423
AhdI GACNNNNNGTC 1 cut(s) 405
AjiI CACGTC 1 cut(s) 808
AluBI AGCT 3 cut(s) 18, 75, 106
AluI AGCT 3 cut(s) 18, 75, 106
Alw21I GWGCWC 3 cut(s) 77, 602, 813
Alw26I GTCTC 2 cut(s) 413, 530
Alw44I GTGCAC 1 cut(s) 809
AlwI GGATC 1 cut(s) 291
AoxI GGCC 4 cut(s) 307, 383, 458, 617
ApaLI GTGCAC 1 cut(s) 809
AspA2I CCTAGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 231, 478
AvrII CCTAGG 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 813
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 2 cut(s) 77, 580
Bbv12I GWGCWC 3 cut(s) 77, 602, 813
BccI CCATC 4 cut(s) 469, 670, 854, 860
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 413, 530
BfaI CTAG 2 cut(s) 197, 311
BlnI CCTAGG 1 cut(s) 196
BmeRI GACNNNNNGTC 1 cut(s) 405
BmgBI CACGTC 1 cut(s) 808
BmiI GGNNCC 3 cut(s) 577, 630, 660
BmsI GCATC 3 cut(s) 73, 469, 505
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 2 cut(s) 4, 110
BsaBI GATNNNNATC 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 820
Bse118I RCCGGY 2 cut(s) 404, 655
Bse8I GATNNNNATC 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 439
BseLI CCNNNNNNNGG 2 cut(s) 305, 820
BseMII CTCAG 2 cut(s) 469, 579
BseRI GAGGAG 4 cut(s) 520, 610, 728, 792
BseSI GKGCMC 1 cut(s) 813
BseYI CCCAGC 1 cut(s) 579
BshFI GGCC 4 cut(s) 309, 385, 460, 619
BshNI GGYRCC 1 cut(s) 658
BsiHKAI GWGCWC 3 cut(s) 77, 602, 813
BsiSI CCGG 4 cut(s) 389, 405, 626, 656
BslI CCNNNNNNNGG 2 cut(s) 305, 820
BsmAI GTCTC 2 cut(s) 413, 530
BsmBI CGTCTC 1 cut(s) 530
BsnI GGCC 4 cut(s) 309, 385, 460, 619
Bso31I GGTCTC 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 77, 580, 602, 813
Bsp143I GATC 4 cut(s) 283, 445, 538, 570
BspACI CCGC 1 cut(s) 373
BspANI GGCC 4 cut(s) 309, 385, 460, 619
BspCNI CTCAG 2 cut(s) 468, 578
BspLI GGNNCC 3 cut(s) 577, 630, 660
BspPI GGATC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 413
BsrFI RCCGGY 2 cut(s) 404, 655
BssAI RCCGGY 2 cut(s) 404, 655
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 4 cut(s) 283, 445, 538, 570
BssNI GRCGYC 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 196
Bst6I CTCTTC 7 cut(s) 693, 700, 705, 720, 755, 762, 767
BstACI GRCGYC 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 808
BstDEI CTNAG 3 cut(s) 455, 565, 614
BstKTI GATC 4 cut(s) 286, 448, 541, 573
BstMAI GTCTC 2 cut(s) 413, 530
BstMBI GATC 4 cut(s) 283, 445, 538, 570
BstMWI GCNNNNNNNGC 1 cut(s) 808
BstSLI GKGCMC 1 cut(s) 813
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 4 cut(s) 309, 385, 460, 619
BtrI CACGTC 1 cut(s) 808
Cfr10I RCCGGY 2 cut(s) 404, 655
CseI GACGC 2 cut(s) 185, 631
CviAII CATG 3 cut(s) 585, 742, 803
DdeI CTNAG 3 cut(s) 455, 565, 614
DpnI GATC 4 cut(s) 285, 447, 540, 572
DpnII GATC 4 cut(s) 283, 445, 538, 570
DriI GACNNNNNGTC 1 cut(s) 405
Eam1104I CTCTTC 7 cut(s) 693, 700, 705, 720, 755, 762, 767
Eam1105I GACNNNNNGTC 1 cut(s) 405
EarI CTCTTC 7 cut(s) 693, 700, 705, 720, 755, 762, 767
Ecl136II GAGCTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 196
Eco24I GRGCYC 2 cut(s) 77, 580
Eco31I GGTCTC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 75
EcoRV GATATC 1 cut(s) 437
EcoT14I CCWWGG 1 cut(s) 196
EcoT38I GRGCYC 2 cut(s) 77, 580
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 3 cut(s) 588, 745, 806
FaiI YATR 7 cut(s) 32, 141, 174, 513, 586, 743, 804
FatI CATG 3 cut(s) 584, 741, 802
FbaI TGATCA 1 cut(s) 538
FriOI GRGCYC 2 cut(s) 77, 580
FspBI CTAG 2 cut(s) 197, 311
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 4 cut(s) 309, 385, 460, 619
HapII CCGG 4 cut(s) 389, 405, 626, 656
HgaI GACGC 2 cut(s) 185, 631
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 3 cut(s) 588, 745, 806
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 4 cut(s) 147, 469, 610, 839
HpaII CCGG 4 cut(s) 389, 405, 626, 656
HphI GGTGA 2 cut(s) 231, 478
Hpy166II GTNNAC 4 cut(s) 146, 203, 239, 811
Hpy188I TCNGA 9 cut(s) 185, 399, 445, 450, 474, 568, 575, 591, 667
Hpy188III TCNNGA 3 cut(s) 44, 232, 287
Hpy8I GTNNAC 4 cut(s) 146, 203, 239, 811
Hpy99I CGWCG 2 cut(s) 595, 625
HpyAV CCTTC 2 cut(s) 50, 429
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 3 cut(s) 428, 482, 811
HpyF10VI GCNNNNNNNGC 1 cut(s) 808
HpyF3I CTNAG 3 cut(s) 455, 565, 614
HpySE526I ACGT 1 cut(s) 807
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 3 cut(s) 588, 745, 806
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 4 cut(s) 283, 445, 538, 570
LmnI GCTCC 3 cut(s) 575, 597, 628
LpnPI CCDG 9 cut(s) 217, 255, 402, 418, 442, 593, 639, 669, 864
LweI GCATC 3 cut(s) 73, 469, 505
MaeI CTAG 2 cut(s) 197, 311
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 3 cut(s) 44, 190, 680
MalI GATC 4 cut(s) 285, 447, 540, 572
MboI GATC 4 cut(s) 283, 445, 538, 570
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 1 cut(s) 283
MhlI GDGCHC 4 cut(s) 77, 580, 602, 813
MluCI AATT 7 cut(s) 92, 131, 265, 301, 315, 423, 542
MlyI GAGTC 3 cut(s) 141, 619, 833
MmeI TCCRAC 2 cut(s) 163, 614
MseI TTAA 2 cut(s) 218, 750
MspI CCGG 4 cut(s) 389, 405, 626, 656
MunI CAATTG 1 cut(s) 423
MwoI GCNNNNNNNGC 1 cut(s) 808
NdeII GATC 4 cut(s) 283, 445, 538, 570
NlaIII CATG 3 cut(s) 588, 745, 806
NlaIV GGNNCC 3 cut(s) 577, 630, 660
NmuCI GTSAC 2 cut(s) 44, 680
PfeI GAWTC 1 cut(s) 469
PflFI GACNNNGTC 1 cut(s) 179
PleI GAGTC 3 cut(s) 141, 618, 833
PpsI GAGTC 3 cut(s) 141, 618, 833
Psp124BI GAGCTC 1 cut(s) 77
PspFI CCCAGC 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 577, 630, 660
PsuI RGATCY 1 cut(s) 283
PsyI GACNNNGTC 1 cut(s) 179
SacI GAGCTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 218, 750
Sau3AI GATC 4 cut(s) 283, 445, 538, 570
SchI GAGTC 3 cut(s) 141, 619, 833
SduI GDGCHC 4 cut(s) 77, 580, 602, 813
SfaNI GCATC 3 cut(s) 73, 469, 505
SmlI CTYRAG 2 cut(s) 19, 125
SmoI CTYRAG 2 cut(s) 19, 125
Sse9I AATT 7 cut(s) 92, 131, 265, 301, 315, 423, 542
SsiI CCGC 1 cut(s) 373
SspMI CTAG 2 cut(s) 197, 311
SstI GAGCTC 1 cut(s) 77
StyI CCWWGG 1 cut(s) 196
TaiI ACGT 1 cut(s) 810
TaqI TCGA 2 cut(s) 366, 608
TaqII GACCGA 1 cut(s) 491
TasI AATT 7 cut(s) 92, 131, 265, 301, 315, 423, 542
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 2 cut(s) 218, 750
Tru9I TTAA 2 cut(s) 218, 750
TseFI GTSAC 2 cut(s) 44, 680
Tsp45I GTSAC 2 cut(s) 44, 680
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 689, 699
Tth111I GACNNNGTC 1 cut(s) 179
VneI GTGCAC 1 cut(s) 809
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 2 cut(s) 197, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.