Rmu_sc0002219.1_g000001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002219.1
N/A
Physical Location & Seq
Reverse (-)
1 .. 1767
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
atgtcttcgttgttcctcatctctgtgttgatatcattgggcactagtattggtatagtccaagcaggcaacccaaattacagagatgcccttgcaaagtccatcctcttcttccagggacagaggtcaggcgagctcccctccggcgctgcccagcaaattacctggaggtccaattctggactttctgatggtcaactcgcaaaagtggacttaactggaggatactatgatggtggagacaatgtcaaattcaacttcccaatggctttcactgcgacaatgctatcatggggatcacttgaatatagcaaaaaaatggagccccaactaaagagtacccgggccgcaatccgatggtccgcaaactaccttctaaagtgtgctcgagccacgcccgataggctctacgttgggatcggggaccctaatgtagaccacgagtgttgggaaaggcccgaagacatggacacggctcgaaccgtgtactgggtctcacctagcaatcctggttcggatgttgctggagagacggcggccgcattggctgctgcttctatggttttccgaagggttgacctcaagtattcaaaattgttattaagaacagccaagaaagtatttcagtttgcaattcagcacaaaggtgcctacagtgattcacttggttctgctgtttgcccattttattgctcatattcaggatacatg

Protein Analysis

237

Amino Acids

25.92

Weight (kDa)

8.81

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 27 - 236 1.1e-78 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 435
AciI CCGC 4 cut(s) 348, 363, 536, 540
AclWI GGATC 2 cut(s) 304, 425
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 2 cut(s) 340, 488
AfiI CCNNNNNNNGG 1 cut(s) 489
AgsI TTSAA 3 cut(s) 256, 305, 591
AhlI ACTAGT 1 cut(s) 44
AjnI CCWGG 3 cut(s) 114, 164, 508
AleI CACNNNNGTG 1 cut(s) 645
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw21I GWGCWC 2 cut(s) 138, 388
Alw26I GTCTC 3 cut(s) 234, 499, 524
AlwI GGATC 2 cut(s) 304, 425
Ama87I CYCGRG 2 cut(s) 342, 387
AoxI GGCC 3 cut(s) 345, 455, 537
ApeKI GCWGC 3 cut(s) 149, 548, 551
ApoI RAATTY 1 cut(s) 251
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 5 cut(s) 171, 345, 360, 424, 456
AsuC2I CCSGG 2 cut(s) 343, 344
AsuHPI GGTGA 1 cut(s) 489
AvaI CYCGRG 2 cut(s) 342, 387
AvaII GGWCC 3 cut(s) 171, 360, 424
BaeGI GKGCMC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 2 cut(s) 138, 327
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 2 cut(s) 138, 388
BbvI GCAGC 3 cut(s) 136, 535, 538
BccI CCATC 4 cut(s) 110, 185, 227, 351
BceAI ACGGC 2 cut(s) 489, 549
BciT130I CCWGG 3 cut(s) 116, 166, 510
BciVI GTATCC 2 cut(s) 218, 698
BcnI CCSGG 2 cut(s) 343, 344
BcoDI GTCTC 3 cut(s) 234, 499, 524
BcuI ACTAGT 1 cut(s) 44
BfaI CTAG 2 cut(s) 45, 501
BfmI CTRYAG 1 cut(s) 652
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 2 cut(s) 218, 698
BglI GCCNNNNNGGC 2 cut(s) 403, 545
BisI GCNGC 6 cut(s) 150, 348, 537, 540, 549, 552
BlsI GCNGC 6 cut(s) 151, 349, 538, 541, 550, 553
Bme1390I CCNGG 5 cut(s) 116, 166, 343, 344, 510
Bme18I GGWCC 3 cut(s) 171, 360, 424
BmeT110I CYCGRG 2 cut(s) 342, 387
BmgT120I GGNCC 5 cut(s) 171, 345, 360, 424, 456
BmiI GGNNCC 4 cut(s) 324, 425, 426, 649
BmrFI CCNGG 5 cut(s) 116, 166, 343, 344, 510
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 76
BmuI ACTGGG 1 cut(s) 499
BoxI GACNNNNGTC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 468
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpmI CTGGAG 3 cut(s) 187, 240, 546
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 2 cut(s) 343, 344
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 115, 342
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse1I ACTGG 2 cut(s) 223, 494
BseBI CCWGG 3 cut(s) 116, 166, 510
BseDI CCNNGG 2 cut(s) 115, 342
BseGI GGATG 2 cut(s) 102, 523
BseLI CCNNNNNNNGG 1 cut(s) 489
BseNI ACTGG 2 cut(s) 223, 494
BseSI GKGCMC 1 cut(s) 44
BseX3I CGGCCG 1 cut(s) 537
BseXI GCAGC 3 cut(s) 136, 535, 538
BseYI CCCAGC 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 540
BshFI GGCC 3 cut(s) 347, 457, 539
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 138, 388
BsiHKCI CYCGRG 2 cut(s) 342, 387
BsiSI CCGG 2 cut(s) 144, 343
BslFI GGGAC 2 cut(s) 132, 437
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 3 cut(s) 234, 499, 524
BsmBI CGTCTC 1 cut(s) 524
BsmFI GGGAC 2 cut(s) 132, 437
BsnI GGCC 3 cut(s) 347, 457, 539
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 2 cut(s) 342, 387
Bsp1286I GDGCHC 4 cut(s) 44, 138, 327, 388
Bsp143I GATC 2 cut(s) 296, 417
BspACI CCGC 4 cut(s) 348, 363, 536, 540
BspANI GGCC 3 cut(s) 347, 457, 539
BspLI GGNNCC 4 cut(s) 324, 425, 426, 649
BspPI GGATC 2 cut(s) 304, 425
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 499
BsrI ACTGG 2 cut(s) 223, 494
BssECI CCNNGG 2 cut(s) 115, 342
BssMI GATC 2 cut(s) 296, 417
BssSI CACGAG 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 3 cut(s) 116, 166, 510
Bst4CI ACNGT 2 cut(s) 484, 656
Bst6I CTCTTC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 67, 134
BstF5I GGATG 2 cut(s) 102, 523
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 2 cut(s) 299, 420
BstMAI GTCTC 3 cut(s) 234, 499, 524
BstMBI GATC 2 cut(s) 296, 417
BstMCI CGRYCG 1 cut(s) 540
BstMWI GCNNNNNNNGC 4 cut(s) 275, 403, 545, 548
BstNI CCWGG 3 cut(s) 116, 166, 510
BstPAI GACNNNNGTC 1 cut(s) 124
BstSCI CCNGG 5 cut(s) 114, 164, 341, 342, 508
BstSFI CTRYAG 1 cut(s) 652
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 136, 535, 538
BstV2I GAAGAC 1 cut(s) 468
BstZI CGGCCG 1 cut(s) 537
BsuI GTATCC 2 cut(s) 218, 698
BsuRI GGCC 3 cut(s) 347, 457, 539
BtsCI GGATG 2 cut(s) 102, 523
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 2 cut(s) 273, 661
Cac8I GCNNGC 2 cut(s) 67, 134
CciNI GCGGCCGC 1 cut(s) 537
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 5 cut(s) 171, 345, 360, 424, 456
Cfr9I CCCGGG 1 cut(s) 342
Csp6I GTAC 2 cut(s) 339, 487
CspCI CAANNNNNGTGG 2 cut(s) 428, 463
CviAII CATG 3 cut(s) 291, 466, 709
CviQI GTAC 2 cut(s) 339, 487
DpnI GATC 2 cut(s) 298, 419
DpnII GATC 2 cut(s) 296, 417
EaeI YGGCCR 1 cut(s) 537
EagI CGGCCG 1 cut(s) 537
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 136
EclXI CGGCCG 1 cut(s) 537
Eco24I GRGCYC 2 cut(s) 138, 327
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 3 cut(s) 171, 360, 424
Eco52I CGGCCG 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 342, 387
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 114, 164, 508
EcoRV GATATC 1 cut(s) 33
EcoT38I GRGCYC 2 cut(s) 138, 327
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 2 cut(s) 294, 469
FaiI YATR 8 cut(s) 56, 231, 292, 309, 467, 560, 697, 710
FaqI GGGAC 2 cut(s) 132, 437
FatI CATG 2 cut(s) 290, 465
FblI GTMKAC 1 cut(s) 435
Fnu4HI GCNGC 6 cut(s) 150, 348, 537, 540, 549, 552
FokI GGATG 2 cut(s) 89, 530
FriOI GRGCYC 2 cut(s) 138, 327
Fsp4HI GCNGC 6 cut(s) 150, 348, 537, 540, 549, 552
FspBI CTAG 2 cut(s) 45, 501
GlaI GCGC 1 cut(s) 148
GluI GCNGC 6 cut(s) 150, 348, 537, 540, 549, 552
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 3 cut(s) 187, 240, 546
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 3 cut(s) 347, 457, 539
HapII CCGG 2 cut(s) 144, 343
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 294, 469
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 2 cut(s) 197, 577
HindII GTYRAC 2 cut(s) 197, 577
HinfI GANTC 1 cut(s) 659
HpaII CCGG 2 cut(s) 144, 343
HphI GGTGA 1 cut(s) 489
Hpy166II GTNNAC 5 cut(s) 197, 211, 436, 487, 577
Hpy188I TCNGA 4 cut(s) 190, 356, 517, 569
Hpy188III TCNNGA 2 cut(s) 180, 702
Hpy8I GTNNAC 5 cut(s) 197, 211, 436, 487, 577
HpyAV CCTTC 2 cut(s) 383, 564
HpyCH4III ACNGT 2 cut(s) 484, 656
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 2 cut(s) 95, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 403, 545, 548
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 2 cut(s) 294, 469
HspAI GCGC 1 cut(s) 147
KflI GGGWCCC 1 cut(s) 424
Kzo9I GATC 2 cut(s) 296, 417
LmnI GCTCC 2 cut(s) 141, 322
Lsp1109I GCAGC 3 cut(s) 136, 535, 538
LweI GCATC 1 cut(s) 76
MaeI CTAG 2 cut(s) 45, 501
MaeII ACGT 1 cut(s) 411
MalI GATC 2 cut(s) 298, 419
MboI GATC 2 cut(s) 296, 417
MboII GAAGA 3 cut(s) 100, 103, 473
MhlI GDGCHC 4 cut(s) 44, 138, 327, 388
MluCI AATT 6 cut(s) 76, 159, 175, 251, 593, 633
MnlI CCTC 7 cut(s) 26, 116, 117, 151, 162, 215, 590
MseI TTAA 2 cut(s) 215, 602
MslI CAYNNNNRTG 2 cut(s) 23, 645
MspI CCGG 2 cut(s) 144, 343
MspR9I CCNGG 5 cut(s) 116, 166, 343, 344, 510
MvaI CCWGG 3 cut(s) 116, 166, 510
MwoI GCNNNNNNNGC 4 cut(s) 275, 403, 545, 548
NciI CCSGG 2 cut(s) 343, 344
NdeII GATC 2 cut(s) 296, 417
NlaIII CATG 2 cut(s) 294, 469
NlaIV GGNNCC 4 cut(s) 324, 425, 426, 649
NotI GCGGCCGC 1 cut(s) 537
OliI CACNNNNGTG 1 cut(s) 645
PaeR7I CTCGAG 1 cut(s) 387
PfeI GAWTC 1 cut(s) 659
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 6 cut(s) 151, 349, 538, 541, 550, 553
PpuMI RGGWCCY 1 cut(s) 424
PshAI GACNNNNGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 3 cut(s) 114, 164, 508
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 114, 164, 508
PspN4I GGNNCC 4 cut(s) 324, 425, 426, 649
PspPI GGNCC 5 cut(s) 171, 345, 360, 424, 456
PspPPI RGGWCCY 1 cut(s) 424
PspXI VCTCGAGB 1 cut(s) 387
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 2 cut(s) 340, 488
RsaNI GTAC 2 cut(s) 339, 487
RseI CAYNNNNRTG 2 cut(s) 23, 645
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 215, 602
SatI GCNGC 6 cut(s) 150, 348, 537, 540, 549, 552
Sau3AI GATC 2 cut(s) 296, 417
Sau96I GGNCC 5 cut(s) 171, 345, 360, 424, 456
ScrFI CCNGG 5 cut(s) 116, 166, 343, 344, 510
SduI GDGCHC 4 cut(s) 44, 138, 327, 388
SetI ASST 9 cut(s) 128, 138, 167, 173, 375, 414, 502, 582, 649
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 387
SinI GGWCC 3 cut(s) 171, 360, 424
SlaI CTCGAG 1 cut(s) 387
SmaI CCCGGG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 23, 645
SmlI CTYRAG 2 cut(s) 387, 581
SmoI CTYRAG 2 cut(s) 387, 581
SpeI ACTAGT 1 cut(s) 44
Sse9I AATT 6 cut(s) 76, 159, 175, 251, 593, 633
SsiI CCGC 4 cut(s) 348, 363, 536, 540
SspMI CTAG 2 cut(s) 45, 501
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 5 cut(s) 114, 164, 341, 342, 508
TaaI ACNGT 2 cut(s) 484, 656
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 388, 478
TasI AATT 6 cut(s) 76, 159, 175, 251, 593, 633
TatI WGTACW 1 cut(s) 486
TauI GCSGC 3 cut(s) 350, 539, 542
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 2 cut(s) 215, 602
Tru9I TTAA 2 cut(s) 215, 602
TscAI CASTG 2 cut(s) 280, 661
TseI GCWGC 3 cut(s) 149, 548, 551
TspMI CCCGGG 1 cut(s) 342
TspRI CASTG 2 cut(s) 280, 661
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 3 cut(s) 171, 360, 424
XapI RAATTY 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 387
XmaI CCCGGG 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 2 cut(s) 45, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.