Rmu_sc0002263.1_g000035

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002263.1
N/A
Physical Location & Seq
Reverse (-)
154241 .. 157676
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002263.1_g000035.1.cds

Sequence Viewer

Length: 1905 bp
atgcggggcataggtaagacaacacttgctcgagctatccatgatgaaatttgtcatgaatttgaccgagtctactttctttccaatgttagagaaaggtctaaaaacaatggcctagtttctcttcaagaaaaacttctttctgaaatcctaatggcaaaaattaaaaatatagaggatgaatacacaggagctgctatgataaaaaggcggtcgtgtagaataaaggtgcttgttgttattgatgatgtggataagttgacacaattagagaacttggctggaagtcgcaactggtttggtccagggagtagaattgtcataaccaccacagatgttcagttgttaaaggcacatgatgttgatgctacatacaaggctaacgggttaaactgtgatgaagcacttcaacttttgagtttgaaggcttttaagaaatgtcccccaccagtagattatttgcatctgtgctacgatattatagggtatgctcaggggcttccgttggctctcgtggttttaggatcttttctatttggtggaagaactgatgaatgggcaagtgcgatagataggctaaagaacacaccagataaacgcattattgatgtgcttcagattagttttgatgaactggatgaaaaagacagagaaatattcctacatattgcttgcttttacaaggggaaggataaggatcgtgtgacacaaatactagactattgccagctaaaccctgtcattggtttgagtgttcttactagactatatccaacaacgaactgtggatgcatgatttgctacaagaattgggctgggaaattgttcgcgaacagtctcctaaagagccaggaaaatggaacagattcaatccaaggcatggtaatggagttgactaaattacaagtggctcattggaagccagaagccttttcaaatttgtctcaacttagtcttctccatattcataatgtggaccttcccaaaggcctcacttgtctttctaattccttaagactcctcgaatggactgagtatcccttgagatctctcccacaaaattttgaagcagatgaacttattgaactcaacttgtgccacagcaacattgaacagctttggaagggaacaaaggctcttgaaagtagtcctcaggagcaacattctctgccagagctgcattctcctccagaacagtgctctcctcaagaacagcaacaaattgactcggatctcttttcttcagattatgatgggagctttctagatggtttttcagatctcgatagctctgataatgaggtaaccattgatcctacatctgagggggagacaaaacttatgacgtccttggttaaaatggggtacaatgaattggaggcttcaatagcaatagaaagatgcgattttgactgctggttcaacttttacagcgtacacatctggcggagtgagtatcctgagaagaataaaaggaaattgtgtgaatcttcattgctgaaaaggaaaaggattatgggacatggaaaccagatcataggagaagatgctggtgcaattcatctcccaagtccaatgaatgggtttgggactccagctgatctctgtcaaatccacagaagtcttccagaggcagccacaggacctccttatttttactatgagaatgtggcattgactcctgaaggagtttggagcacaatatcccgctttttgtatgacgtgaagcctgagtttgttgatttaaagtatttttgtgctgcagcaagaaaaaggggttatgttcacaatcttccaatcaaaaacagaattcgtcttgttccccttccaccacaaactatatttgatgtatttcccatgacaaggaggtggtggcctacttgggatgatcggacaaaaagctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

634

Amino Acids

72.21

Weight (kDa)

5.74

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1349
AccB7I CCANNNNNTGG 2 cut(s) 880, 1580
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 830
AciI CCGC 4 cut(s) 4, 211, 1447, 1708
AclWI GGATC 4 cut(s) 532, 705, 1239, 1307
AcsI RAATTY 5 cut(s) 48, 59, 937, 1060, 1809
AcuI CTGAAG 3 cut(s) 599, 1227, 1704
AcyI GRCGYC 1 cut(s) 1346
AfaI GTAC 2 cut(s) 1367, 1437
AfiI CCNNNNNNNGG 4 cut(s) 743, 880, 1580, 1863
AflII CTTAAG 1 cut(s) 1012
AjiI CACGTC 1 cut(s) 1723
AjnI CCWGG 2 cut(s) 304, 849
AluBI AGCT 9 cut(s) 35, 194, 730, 1117, 1177, 1260, 1290, 1598, 1902
AluI AGCT 9 cut(s) 35, 194, 730, 1117, 1177, 1260, 1290, 1598, 1902
Alw21I GWGCWC 2 cut(s) 1202, 1700
Alw26I GTCTC 3 cut(s) 842, 948, 1325
AlwI GGATC 4 cut(s) 532, 705, 1239, 1307
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 3 cut(s) 112, 988, 1874
ApeKI GCWGC 5 cut(s) 194, 1177, 1634, 1760, 1763
ApoI RAATTY 5 cut(s) 48, 59, 937, 1060, 1809
Asp700I GAANNNNTTC 4 cut(s) 135, 405, 824, 865
AspS9I GGNCC 3 cut(s) 302, 976, 1643
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 3 cut(s) 302, 976, 1643
AxyI CCTNAGG 1 cut(s) 1152
BauI CACGAG 1 cut(s) 512
BbsI GAAGAC 2 cut(s) 947, 1616
Bbv12I GWGCWC 2 cut(s) 1202, 1700
BbvI GCAGC 5 cut(s) 181, 1164, 1646, 1747, 1775
BccI CCATC 2 cut(s) 1247, 1262
BciT130I CCWGG 2 cut(s) 306, 851
BciVI GTATCC 2 cut(s) 1047, 1467
BcoDI GTCTC 3 cut(s) 842, 948, 1325
BfaI CTAG 4 cut(s) 116, 716, 762, 1265
BfmI CTRYAG 1 cut(s) 1761
BfrI CTTAAG 1 cut(s) 1012
BfuI GTATCC 2 cut(s) 1047, 1467
BglII AGATCT 2 cut(s) 1046, 1279
BisI GCNGC 5 cut(s) 195, 1178, 1635, 1761, 1764
BlsI GCNGC 5 cut(s) 196, 1179, 1636, 1762, 1765
Bme1390I CCNGG 2 cut(s) 306, 851
Bme18I GGWCC 3 cut(s) 302, 976, 1643
BmeT110I CYCGRG 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 1723
BmgT120I GGNCC 3 cut(s) 302, 976, 1643
BmrFI CCNGG 2 cut(s) 306, 851
BmsI GCATC 5 cut(s) 355, 472, 779, 1391, 1537
BpiI GAAGAC 2 cut(s) 947, 1616
BpmI CTGGAG 2 cut(s) 1173, 1578
Bpu10I CCTNAGC 1 cut(s) 492
BpuEI CTTGAG 2 cut(s) 1063, 1191
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 1346
BsaJI CCNNGG 3 cut(s) 305, 874, 1350
BsaXI ACNNNNNCTCC 2 cut(s) 1630, 1660
Bsc4I CCNNNNNNNGG 4 cut(s) 743, 880, 1580, 1863
Bse1I ACTGG 3 cut(s) 299, 449, 639
Bse21I CCTNAGG 1 cut(s) 1152
Bse3DI GCAATG 1 cut(s) 1493
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 2 cut(s) 306, 851
BseDI CCNNGG 3 cut(s) 305, 874, 1350
BseGI GGATG 4 cut(s) 184, 643, 794, 1891
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 4 cut(s) 743, 880, 1580, 1863
BseMI GCAATG 1 cut(s) 1493
BseMII CTCAG 6 cut(s) 506, 1023, 1166, 1314, 1452, 1722
BseNI ACTGG 3 cut(s) 299, 449, 639
BseRI GAGGAG 3 cut(s) 1010, 1176, 1194
BseXI GCAGC 5 cut(s) 181, 1164, 1646, 1747, 1775
BseYI CCCAGC 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 830
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 3 cut(s) 114, 990, 1876
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 2 cut(s) 1202, 1700
BsiHKCI CYCGRG 1 cut(s) 30
BslFI GGGAC 3 cut(s) 426, 1533, 1603
BslI CCNNNNNNNGG 4 cut(s) 743, 880, 1580, 1863
BsmAI GTCTC 3 cut(s) 842, 948, 1325
BsmFI GGGAC 3 cut(s) 426, 1533, 1603
BsmI GAATGC 1 cut(s) 1180
BsnI GGCC 3 cut(s) 114, 990, 1876
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 1202, 1700
Bsp143I GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
Bsp68I TCGCGA 1 cut(s) 830
BspACI CCGC 4 cut(s) 4, 211, 1447, 1708
BspANI GGCC 3 cut(s) 114, 990, 1876
BspCNI CTCAG 6 cut(s) 505, 1024, 1165, 1315, 1453, 1723
BspFNI CGCG 1 cut(s) 830
BspHI TCATGA 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 1765
BspPI GGATC 4 cut(s) 532, 705, 1239, 1307
BspTI CTTAAG 1 cut(s) 1012
BsrDI GCAATG 1 cut(s) 1493
BsrI ACTGG 3 cut(s) 299, 449, 639
BssECI CCNNGG 3 cut(s) 305, 874, 1350
BssMI GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
BssNI GRCGYC 1 cut(s) 1346
BssSI CACGAG 1 cut(s) 512
BssT1I CCWWGG 2 cut(s) 874, 1350
Bst2BI CACGAG 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 306, 851
Bst4CI ACNGT 4 cut(s) 395, 785, 836, 1197
Bst6I CTCTTC 1 cut(s) 129
BstACI GRCGYC 1 cut(s) 1346
BstAFI CTTAAG 1 cut(s) 1012
BstAPI GCANNNNNTGC 1 cut(s) 798
BstC8I GCNNGC 2 cut(s) 673, 728
BstDEI CTNAG 7 cut(s) 492, 950, 1032, 1152, 1323, 1461, 1731
BstEII GGTNACC 1 cut(s) 1303
BstF5I GGATG 4 cut(s) 184, 643, 794, 1891
BstFNI CGCG 1 cut(s) 830
BstKTI GATC 9 cut(s) 527, 700, 1049, 1234, 1282, 1315, 1536, 1603, 1891
BstMAI GTCTC 3 cut(s) 842, 948, 1325
BstMBI GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 3 cut(s) 798, 1177, 1388
BstNI CCWGG 2 cut(s) 306, 851
BstPI GGTNACC 1 cut(s) 1303
BstSCI CCNGG 2 cut(s) 304, 849
BstSFI CTRYAG 1 cut(s) 1761
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 5 cut(s) 181, 1164, 1646, 1747, 1775
BstV2I GAAGAC 2 cut(s) 947, 1616
BstX2I RGATCY 4 cut(s) 524, 1046, 1231, 1279
BstXI CCANNNNNNTGG 1 cut(s) 857
BstYI RGATCY 4 cut(s) 524, 1046, 1231, 1279
Bsu36I CCTNAGG 1 cut(s) 1152
BsuI GTATCC 2 cut(s) 1047, 1467
BsuRI GGCC 3 cut(s) 114, 990, 1876
BtrI CACGTC 1 cut(s) 1723
BtsCI GGATG 4 cut(s) 184, 643, 794, 1891
BtsIMutI CAGTG 1 cut(s) 1202
BtuMI TCGCGA 1 cut(s) 830
Cac8I GCNNGC 2 cut(s) 673, 728
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 302, 976, 1643
Csp6I GTAC 2 cut(s) 1366, 1436
CviAII CATG 7 cut(s) 41, 56, 356, 793, 880, 1523, 1858
CviQI GTAC 2 cut(s) 1366, 1436
DdeI CTNAG 7 cut(s) 492, 950, 1032, 1152, 1323, 1461, 1731
DpnI GATC 9 cut(s) 526, 699, 1048, 1233, 1281, 1314, 1535, 1602, 1890
DpnII GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
DraI TTTAAA 1 cut(s) 1746
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 1462
Eco130I CCWWGG 2 cut(s) 874, 1350
Eco147I AGGCCT 1 cut(s) 990
Eco47I GGWCC 3 cut(s) 302, 976, 1643
Eco57I CTGAAG 3 cut(s) 599, 1227, 1704
Eco81I CCTNAGG 1 cut(s) 1152
Eco88I CYCGRG 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 1303
EcoO109I RGGNCCY 1 cut(s) 1643
EcoO65I GGTNACC 1 cut(s) 1303
EcoRI GAATTC 1 cut(s) 1809
EcoRII CCWGG 2 cut(s) 304, 849
EcoT14I CCWWGG 2 cut(s) 874, 1350
EcoT22I ATGCAT 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 874, 1350
FaeI CATG 7 cut(s) 44, 59, 359, 796, 883, 1526, 1861
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 3 cut(s) 426, 1533, 1603
FatI CATG 7 cut(s) 40, 55, 355, 792, 879, 1522, 1857
FauI CCCGC 1 cut(s) 1715
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 195, 1178, 1635, 1761, 1764
FokI GGATG 4 cut(s) 191, 650, 801, 1898
Fsp4HI GCNGC 5 cut(s) 195, 1178, 1635, 1761, 1764
FspBI CTAG 4 cut(s) 116, 716, 762, 1265
GluI GCNGC 5 cut(s) 195, 1178, 1635, 1761, 1764
GsaI CCCAGC 1 cut(s) 819
GsuI CTGGAG 2 cut(s) 1173, 1578
HaeIII GGCC 3 cut(s) 114, 990, 1876
Hin1I GRCGYC 1 cut(s) 1346
Hin1II CATG 7 cut(s) 44, 59, 359, 796, 883, 1526, 1861
HincII GTYRAC 2 cut(s) 261, 894
HindII GTYRAC 2 cut(s) 261, 894
HinfI GANTC 7 cut(s) 69, 866, 1017, 1226, 1487, 1591, 1678
Hpy166II GTNNAC 6 cut(s) 73, 261, 894, 976, 1438, 1786
Hpy188I TCNGA 8 cut(s) 145, 618, 1231, 1246, 1279, 1294, 1324, 1893
Hpy8I GTNNAC 6 cut(s) 73, 261, 894, 976, 1438, 1786
HpyAV CCTTC 6 cut(s) 418, 682, 989, 1117, 1679, 1835
HpyCH4III ACNGT 4 cut(s) 395, 785, 836, 1197
HpyCH4IV ACGT 2 cut(s) 1346, 1722
HpyCH4V TGCA 5 cut(s) 463, 792, 1180, 1556, 1763
HpyF10VI GCNNNNNNNGC 3 cut(s) 798, 1177, 1388
HpyF3I CTNAG 7 cut(s) 492, 950, 1032, 1152, 1323, 1461, 1731
HpySE526I ACGT 2 cut(s) 1346, 1722
Hsp92I GRCGYC 1 cut(s) 1346
Hsp92II CATG 7 cut(s) 44, 59, 359, 796, 883, 1526, 1861
Kzo9I GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
LmnI GCTCC 4 cut(s) 191, 1156, 1257, 1695
Lsp1109I GCAGC 5 cut(s) 181, 1164, 1646, 1747, 1775
LweI GCATC 5 cut(s) 355, 472, 779, 1391, 1537
MaeI CTAG 4 cut(s) 116, 716, 762, 1265
MaeII ACGT 2 cut(s) 1346, 1722
MaeIII GTNAC 2 cut(s) 703, 1303
MalI GATC 9 cut(s) 526, 699, 1048, 1233, 1281, 1314, 1535, 1602, 1890
MboI GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
MboII GAAGA 9 cut(s) 116, 555, 947, 1233, 1477, 1482, 1556, 1616, 1784
MflI RGATCY 4 cut(s) 524, 1046, 1231, 1279
MhlI GDGCHC 2 cut(s) 1202, 1700
MlyI GAGTC 5 cut(s) 78, 1011, 1220, 1585, 1672
MmeI TCCRAC 1 cut(s) 797
Mph1103I ATGCAT 1 cut(s) 794
MroXI GAANNNNTTC 4 cut(s) 135, 405, 824, 865
MseI TTAA 7 cut(s) 165, 347, 389, 432, 1013, 1356, 1745
MslI CAYNNNNRTG 1 cut(s) 884
MspA1I CMGCKG 1 cut(s) 1598
MspCI CTTAAG 1 cut(s) 1012
MspR9I CCNGG 2 cut(s) 306, 851
Mva1269I GAATGC 1 cut(s) 1180
MvaI CCWGG 2 cut(s) 306, 851
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 3 cut(s) 798, 1177, 1388
NdeII GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
NlaIII CATG 7 cut(s) 44, 59, 359, 796, 883, 1526, 1861
NmuCI GTSAC 1 cut(s) 703
NruI TCGCGA 1 cut(s) 830
NsiI ATGCAT 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 1 cut(s) 55
PceI AGGCCT 1 cut(s) 990
PctI GAATGC 1 cut(s) 1180
PdmI GAANNNNTTC 4 cut(s) 135, 405, 824, 865
PfeI GAWTC 2 cut(s) 866, 1487
PflFI GACNNNGTC 1 cut(s) 68
PflMI CCANNNNNTGG 2 cut(s) 880, 1580
PkrI GCNGC 5 cut(s) 196, 1179, 1636, 1762, 1765
PleI GAGTC 5 cut(s) 77, 1011, 1220, 1585, 1672
PpsI GAGTC 5 cut(s) 77, 1011, 1220, 1585, 1672
PpuMI RGGWCCY 1 cut(s) 1643
Psp5II RGGWCCY 1 cut(s) 1643
Psp6I CCWGG 2 cut(s) 304, 849
PspEI GGTNACC 1 cut(s) 1303
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 2 cut(s) 304, 849
PspPI GGNCC 3 cut(s) 302, 976, 1643
PspPPI RGGWCCY 1 cut(s) 1643
PspXI VCTCGAGB 1 cut(s) 30
PstI CTGCAG 1 cut(s) 1765
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 4 cut(s) 524, 1046, 1231, 1279
PsyI GACNNNGTC 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 1598
RruI TCGCGA 1 cut(s) 830
RsaI GTAC 2 cut(s) 1367, 1437
RsaNI GTAC 2 cut(s) 1366, 1436
RseI CAYNNNNRTG 1 cut(s) 884
SaqAI TTAA 7 cut(s) 165, 347, 389, 432, 1013, 1356, 1745
SatI GCNGC 5 cut(s) 195, 1178, 1635, 1761, 1764
Sau3AI GATC 9 cut(s) 524, 697, 1046, 1231, 1279, 1312, 1533, 1600, 1888
Sau96I GGNCC 3 cut(s) 302, 976, 1643
SchI GAGTC 5 cut(s) 78, 1011, 1220, 1585, 1672
ScrFI CCNGG 2 cut(s) 306, 851
SduI GDGCHC 2 cut(s) 1202, 1700
SfaNI GCATC 5 cut(s) 355, 472, 779, 1391, 1537
SfcI CTRYAG 1 cut(s) 1761
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 3 cut(s) 302, 976, 1643
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 4 cut(s) 30, 1012, 1042, 1206
SmoI CTYRAG 4 cut(s) 30, 1012, 1042, 1206
SseBI AGGCCT 1 cut(s) 990
SsiI CCGC 4 cut(s) 4, 211, 1447, 1708
SspI AATATT 1 cut(s) 657
SspMI CTAG 4 cut(s) 116, 716, 762, 1265
StuI AGGCCT 1 cut(s) 990
StyD4I CCNGG 2 cut(s) 304, 849
StyI CCWWGG 2 cut(s) 874, 1350
TaaI ACNGT 4 cut(s) 395, 785, 836, 1197
TaiI ACGT 2 cut(s) 1349, 1725
TaqI TCGA 3 cut(s) 31, 1023, 1284
TaqII GACCGA 1 cut(s) 81
TfiI GAWTC 2 cut(s) 866, 1487
Tru1I TTAA 7 cut(s) 165, 347, 389, 432, 1013, 1356, 1745
Tru9I TTAA 7 cut(s) 165, 347, 389, 432, 1013, 1356, 1745
TscAI CASTG 1 cut(s) 1202
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 5 cut(s) 194, 1177, 1634, 1760, 1763
Tsp45I GTSAC 1 cut(s) 703
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 1 cut(s) 1202
Tth111I GACNNNGTC 1 cut(s) 68
Van91I CCANNNNNTGG 2 cut(s) 880, 1580
Vha464I CTTAAG 1 cut(s) 1012
VpaK11BI GGWCC 3 cut(s) 302, 976, 1643
XapI RAATTY 5 cut(s) 48, 59, 937, 1060, 1809
XbaI TCTAGA 1 cut(s) 1264
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 4 cut(s) 135, 405, 824, 865
XspI CTAG 4 cut(s) 116, 716, 762, 1265
ZraI GACGTC 1 cut(s) 1347
Zsp2I ATGCAT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.