Rmu_sc0002301.1_g000004

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002301.1
N/A
Physical Location & Seq
Forward (+)
13785 .. 16003
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
atggctggtggtggcggcaaaacgaatcagaggggaaagcttcaagagataagagggatatctactatcgaaaagccaaagaagaaggctggcgagctcgcagtgccttcaaagcttcttcagattgacgacgaattcaacatctttcatggagtgaagcgcgtcgtcgatctctgtgctgcccctggtagctggagtcaggttttgagtaggaaattgtatctcccagcaaagctttcatctgattccaaggacggggatgtgccccttattgtggctattgatttgcagccaatggctccaattgaaggtgttattcaagtgcagggtgatataaccaatgcacgaactgccgaagtagtcatcagacattttgatggttgcaaggctgatctggttgtgtgtgatggtgctccagatgttaccggccttcatgacatggatgaatttgttcagtcccagctgatacttgcaggcttaacaattgttactcatgtacttaaagaaggtggtaaatttattgcaaagatattccgtggaaaagatactagtcttctctattgtcagctgaaagtatttttcccaattgtgacttttgcaaaaccaaaaagcagccgcaattccagcataggtattatattggcatttgcggtttgtgagaattattctcctcctgagggattcaaccctaaggatctgcatcgcctcctagaaaaggtgggaagtccctcaggagcaggtatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

248

Amino Acids

26.69

Weight (kDa)

8.53

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 38 - 209 5.2e-43 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 728
AccII CGCG 1 cut(s) 162
AciI CCGC 3 cut(s) 15, 616, 650
AclWI GGATC 1 cut(s) 702
AcsI RAATTY 3 cut(s) 134, 446, 515
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 5 cut(s) 255, 274, 308, 677, 715
AgsI TTSAA 6 cut(s) 44, 111, 139, 308, 320, 685
AhlI ACTAGT 1 cut(s) 548
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 7 cut(s) 40, 97, 115, 192, 235, 463, 568
AluI AGCT 7 cut(s) 40, 97, 115, 192, 235, 463, 568
Alw21I GWGCWC 2 cut(s) 99, 415
AlwI GGATC 1 cut(s) 702
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 3 cut(s) 179, 289, 612
ApoI RAATTY 3 cut(s) 134, 446, 515
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 341
AxyI CCTNAGG 3 cut(s) 675, 690, 730
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 2 cut(s) 99, 415
BbvI GCAGC 3 cut(s) 166, 301, 624
BccI CCATC 2 cut(s) 371, 401
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 186
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 3 cut(s) 549, 710, 745
BfuAI ACCTGC 1 cut(s) 728
BisI GCNGC 5 cut(s) 16, 180, 290, 613, 616
BlsI GCNGC 5 cut(s) 17, 181, 291, 614, 617
Bme1390I CCNGG 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 709
BpiI GAAGAC 1 cut(s) 545
BpmI CTGGAG 2 cut(s) 214, 399
BsaBI GATNNNNATC 1 cut(s) 699
BsaJI CCNNGG 3 cut(s) 184, 249, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 274, 308, 677, 715
Bse118I RCCGGY 1 cut(s) 425
Bse21I CCTNAGG 3 cut(s) 675, 690, 730
Bse8I GATNNNNATC 1 cut(s) 699
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 184, 249, 535
BseGI GGATG 2 cut(s) 265, 448
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 5 cut(s) 255, 274, 308, 677, 715
BseMII CTCAG 2 cut(s) 666, 744
BseRI GAGGAG 1 cut(s) 660
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 3 cut(s) 166, 301, 624
BseYI CCCAGC 2 cut(s) 226, 459
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 429
BsiHKAI GWGCWC 2 cut(s) 99, 415
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 2 cut(s) 442, 711
BslI CCNNNNNNNGG 5 cut(s) 255, 274, 308, 677, 715
BsmFI GGGAC 2 cut(s) 442, 711
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 3 cut(s) 99, 267, 415
Bsp143I GATC 3 cut(s) 169, 391, 694
BspACI CCGC 3 cut(s) 15, 616, 650
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 667, 743
BspFNI CGCG 1 cut(s) 162
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 1 cut(s) 300
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 1 cut(s) 702
BsrFI RCCGGY 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 3 cut(s) 184, 249, 535
BssMI GATC 3 cut(s) 169, 391, 694
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 4 cut(s) 91, 95, 99, 475
BstDEI CTNAG 3 cut(s) 675, 690, 730
BstDSI CCRYGG 1 cut(s) 535
BstENI CCTNNNNNAGG 1 cut(s) 713
BstF5I GGATG 2 cut(s) 265, 448
BstFNI CGCG 1 cut(s) 162
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 3 cut(s) 172, 394, 697
BstMBI GATC 3 cut(s) 169, 391, 694
BstMWI GCNNNNNNNGC 4 cut(s) 103, 112, 350, 624
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 166, 301, 624
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
Bsu36I CCTNAGG 3 cut(s) 675, 690, 730
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 535
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 2 cut(s) 265, 448
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 4 cut(s) 91, 95, 99, 475
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 162
Cfr10I RCCGGY 1 cut(s) 425
CseI GACGC 1 cut(s) 151
Csp6I GTAC 1 cut(s) 497
CviAII CATG 4 cut(s) 149, 434, 439, 494
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 3 cut(s) 675, 690, 730
DpnI GATC 3 cut(s) 171, 393, 696
DpnII GATC 3 cut(s) 169, 391, 694
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 104
Eco81I CCTNAGG 3 cut(s) 675, 690, 730
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 184
EcoRV GATATC 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 4 cut(s) 152, 437, 442, 497
FaiI YATR 7 cut(s) 150, 335, 435, 440, 495, 629, 638
FaqI GGGAC 2 cut(s) 442, 711
FatI CATG 4 cut(s) 148, 433, 438, 493
Fnu4HI GCNGC 5 cut(s) 16, 180, 290, 613, 616
FokI GGATG 2 cut(s) 272, 455
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 5 cut(s) 16, 180, 290, 613, 616
FspBI CTAG 3 cut(s) 549, 710, 745
GlaI GCGC 1 cut(s) 161
GluI GCNGC 5 cut(s) 16, 180, 290, 613, 616
GsaI CCCAGC 2 cut(s) 230, 463
GsuI CTGGAG 2 cut(s) 214, 399
HaeIII GGCC 1 cut(s) 429
HapII CCGG 1 cut(s) 426
HgaI GACGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 162
Hin1II CATG 4 cut(s) 152, 437, 442, 497
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HindIII AAGCTT 3 cut(s) 38, 113, 233
HinfI GANTC 4 cut(s) 25, 196, 245, 681
HpaII CCGG 1 cut(s) 426
HphI GGTGA 1 cut(s) 341
Hpy188I TCNGA 4 cut(s) 30, 123, 244, 368
Hpy188III TCNNGA 5 cut(s) 44, 416, 434, 674, 732
Hpy99I CGWCG 3 cut(s) 134, 167, 170
HpyAV CCTTC 5 cut(s) 79, 117, 302, 440, 500
HpyCH4V TGCA 8 cut(s) 289, 325, 344, 384, 473, 524, 599, 700
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 112, 350, 624
HpyF3I CTNAG 3 cut(s) 675, 690, 730
Hsp92II CATG 4 cut(s) 152, 437, 442, 497
HspAI GCGC 1 cut(s) 160
Kzo9I GATC 3 cut(s) 169, 391, 694
LmnI GCTCC 3 cut(s) 304, 418, 734
Lsp1109I GCAGC 3 cut(s) 166, 301, 624
LweI GCATC 1 cut(s) 709
MaeI CTAG 3 cut(s) 549, 710, 745
MaeIII GTNAC 3 cut(s) 421, 487, 589
MalI GATC 3 cut(s) 171, 393, 696
MboI GATC 3 cut(s) 169, 391, 694
MboII GAAGA 3 cut(s) 94, 110, 545
MfeI CAATTG 3 cut(s) 303, 483, 585
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 3 cut(s) 99, 267, 415
MluCI AATT 9 cut(s) 134, 215, 303, 446, 483, 515, 585, 619, 661
MlyI GAGTC 1 cut(s) 205
MnlI CCTC 6 cut(s) 24, 47, 670, 681, 716, 739
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 2 cut(s) 479, 501
MslI CAYNNNNRTG 1 cut(s) 375
MspA1I CMGCKG 2 cut(s) 463, 568
MspI CCGG 1 cut(s) 426
MspR9I CCNGG 1 cut(s) 186
MunI CAATTG 3 cut(s) 303, 483, 585
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 4 cut(s) 103, 112, 350, 624
NdeII GATC 3 cut(s) 169, 391, 694
NlaIII CATG 4 cut(s) 152, 437, 442, 497
NlaIV GGNNCC 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 589
PagI TCATGA 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 3 cut(s) 25, 245, 681
PkrI GCNGC 5 cut(s) 17, 181, 291, 614, 617
PleI GAGTC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 184
PspFI CCCAGC 2 cut(s) 226, 459
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 2 cut(s) 463, 568
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 375
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 479, 501
SatI GCNGC 5 cut(s) 16, 180, 290, 613, 616
Sau3AI GATC 3 cut(s) 169, 391, 694
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 3 cut(s) 99, 267, 415
SfaNI GCATC 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 375
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 9 cut(s) 134, 215, 303, 446, 483, 515, 585, 619, 661
SsiI CCGC 3 cut(s) 15, 616, 650
SspMI CTAG 3 cut(s) 549, 710, 745
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 249
TaqI TCGA 2 cut(s) 69, 168
TasI AATT 9 cut(s) 134, 215, 303, 446, 483, 515, 585, 619, 661
TatI WGTACW 1 cut(s) 496
TauI GCSGC 2 cut(s) 18, 618
TfiI GAWTC 3 cut(s) 25, 245, 681
Tru1I TTAA 2 cut(s) 479, 501
Tru9I TTAA 2 cut(s) 479, 501
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 3 cut(s) 179, 289, 612
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 4 cut(s) 137, 228, 422, 459
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 713
XapI RAATTY 3 cut(s) 134, 446, 515
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 3 cut(s) 549, 710, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.