Rmu_sc0002556.1_g000018

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002556.1
N/A
Physical Location & Seq
Forward (+)
61306 .. 63822
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
atggatgtttgggaattggcagtttctgtgagtgaggtggagtcttcgtatgagctatacaagaaattaagcagctctgtagttaaagatgggcttatacacaaggaagagctccagcttgcgctttttcagagcagcagtaagcagaacctctttttcgacaggatatttgatcttttcgatgtcaataaaaatgagcatattgagtttgaagagtttgttcatacattgagtgttttccaccctaaaactcctgatgaagtcaaaattacatatgcttttaaattatatgatttgaggcacactggttacatcgagcgtgaggaggcaagtccactgtattcttactttgggggaaaaaatccggtgtcctattgccctattggttacttatccgcaatgcctctggactttgccttcttcgagtgtctgagaccgatcagggccagctcgggtgatctctttgttccccagcatccatttgtagacattgataatttgatcgactgctcgacaacttctgtaagaggagttgggcaccatgtagatggagctatggaggttctaagtagatatgttcagtctccttaa

Protein Analysis

196

Amino Acids

22.43

Weight (kDa)

5.01

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 537
AccI GTMKAC 1 cut(s) 486
AciI CCGC 1 cut(s) 396
AgsI TTSAA 1 cut(s) 212
AluBI AGCT 6 cut(s) 55, 75, 112, 118, 450, 554
AluI AGCT 6 cut(s) 55, 75, 112, 118, 450, 554
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 72, 135
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 540
BanI GGYRCC 1 cut(s) 537
BanII GRGCYC 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 84, 147
BccI CCATC 2 cut(s) 83, 542
BcoDI GTCTC 1 cut(s) 427
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 2 cut(s) 73, 136
BlsI GCNGC 2 cut(s) 74, 137
BmeT110I CYCGRG 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 444
BmiI GGNNCC 1 cut(s) 539
BmsI GCATC 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 405
BseGI GGATG 2 cut(s) 10, 475
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 2 cut(s) 338, 543
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 2 cut(s) 84, 147
BseYI CCCAGC 1 cut(s) 471
BshFI GGCC 1 cut(s) 446
BshNI GGYRCC 1 cut(s) 537
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 1 cut(s) 365
BsmAI GTCTC 1 cut(s) 427
BsnI GGCC 1 cut(s) 446
Bso31I GGTCTC 1 cut(s) 427
BsoBI CYCGRG 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 114, 540
Bsp143I GATC 4 cut(s) 172, 438, 457, 501
BspACI CCGC 1 cut(s) 396
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 539
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 537
BspTNI GGTCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 1 cut(s) 310
BssMI GATC 4 cut(s) 172, 438, 457, 501
Bst4CI ACNGT 1 cut(s) 339
Bst6I CTCTTC 2 cut(s) 102, 207
BstC8I GCNNGC 2 cut(s) 120, 448
BstDEI CTNAG 2 cut(s) 431, 566
BstF5I GGATG 2 cut(s) 10, 475
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 4 cut(s) 175, 441, 460, 504
BstMAI GTCTC 1 cut(s) 427
BstMBI GATC 4 cut(s) 172, 438, 457, 501
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 84, 147
BstV2I GAAGAC 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 548
BsuRI GGCC 1 cut(s) 446
BtsCI GGATG 2 cut(s) 10, 475
BtsIMutI CAGTG 2 cut(s) 303, 335
Cac8I GCNNGC 2 cut(s) 120, 448
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 444
CviAII CATG 1 cut(s) 542
CviJI RGCY 8 cut(s) 55, 75, 94, 112, 118, 446, 450, 554
CviKI_1 RGCY 8 cut(s) 55, 75, 94, 112, 118, 446, 450, 554
DdeI CTNAG 2 cut(s) 431, 566
DpnI GATC 4 cut(s) 174, 440, 459, 503
DpnII GATC 4 cut(s) 172, 438, 457, 501
DraI TTTAAA 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 102, 207
EarI CTCTTC 2 cut(s) 102, 207
Ecl136II GAGCTC 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 114
FaeI CATG 1 cut(s) 545
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 1 cut(s) 541
FauNDI CATATG 1 cut(s) 274
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 73, 136
FokI GGATG 2 cut(s) 17, 462
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 73, 136
GlaI GCGC 1 cut(s) 123
GluI GCNGC 2 cut(s) 73, 136
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 98
HaeIII GGCC 1 cut(s) 446
HapII CCGG 1 cut(s) 365
HhaI GCGC 1 cut(s) 124
Hin1II CATG 1 cut(s) 545
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HinfI GANTC 1 cut(s) 41
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 2 cut(s) 335, 487
Hpy188I TCNGA 2 cut(s) 132, 432
Hpy188III TCNNGA 2 cut(s) 254, 407
Hpy8I GTNNAC 2 cut(s) 335, 487
HpyAV CCTTC 1 cut(s) 427
HpyCH4III ACNGT 1 cut(s) 339
HpyF3I CTNAG 2 cut(s) 431, 566
Hsp92II CATG 1 cut(s) 545
HspAI GCGC 1 cut(s) 122
Kzo9I GATC 4 cut(s) 172, 438, 457, 501
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 117, 551
LpnPI CCDG 9 cut(s) 128, 148, 267, 291, 378, 392, 427, 460, 485
Lsp1109I GCAGC 2 cut(s) 84, 147
LweI GCATC 1 cut(s) 484
MaeIII GTNAC 2 cut(s) 308, 386
MalI GATC 4 cut(s) 174, 440, 459, 503
MboI GATC 4 cut(s) 172, 438, 457, 501
MboII GAAGA 4 cut(s) 36, 119, 224, 412
MhlI GDGCHC 2 cut(s) 114, 540
MluCI AATT 5 cut(s) 14, 65, 267, 284, 496
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 8 cut(s) 28, 161, 291, 316, 319, 414, 521, 553
MseI TTAA 4 cut(s) 68, 84, 282, 589
MslI CAYNNNNRTG 1 cut(s) 546
MspI CCGG 1 cut(s) 365
NdeI CATATG 1 cut(s) 274
NdeII GATC 4 cut(s) 172, 438, 457, 501
NlaIII CATG 1 cut(s) 545
NlaIV GGNNCC 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 102
PkrI GCNGC 2 cut(s) 74, 137
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 539
PspPI GGNCC 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 546
SacI GAGCTC 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 4 cut(s) 68, 84, 282, 589
SatI GCNGC 2 cut(s) 73, 136
Sau3AI GATC 4 cut(s) 172, 438, 457, 501
Sau96I GGNCC 1 cut(s) 444
SchI GAGTC 1 cut(s) 50
SduI GDGCHC 2 cut(s) 114, 540
SetI ASST 9 cut(s) 39, 57, 77, 114, 120, 153, 452, 556, 564
SfaNI GCATC 1 cut(s) 484
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 546
Sse9I AATT 5 cut(s) 14, 65, 267, 284, 496
SsiI CCGC 1 cut(s) 396
SstI GAGCTC 1 cut(s) 114
TaaI ACNGT 1 cut(s) 339
TaqI TCGA 6 cut(s) 159, 180, 315, 423, 504, 512
TaqII GACCGA 1 cut(s) 451
TasI AATT 5 cut(s) 14, 65, 267, 284, 496
Tru1I TTAA 4 cut(s) 68, 84, 282, 589
Tru9I TTAA 4 cut(s) 68, 84, 282, 589
TscAI CASTG 2 cut(s) 310, 342
TseI GCWGC 2 cut(s) 72, 135
TspDTI ATGAA 2 cut(s) 212, 273
TspRI CASTG 2 cut(s) 310, 342
XmiI GTMKAC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.