Rmu_sc0002558.1_g000003

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002558.1
N/A
Physical Location & Seq
Forward (+)
5214 .. 5876
663 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
atggtaggtaattttattaccagattctgtatcagagagtatctttcaaaatctgacaaagtgccaagtgagttgcgttcattctacggcgagcacgtgactagagtgttggaattagcagctctgggttggttcgactgtcggagctccgtttgtttttgctgcaccctcaaactttggctttcctcaacctgctcattctttgcttctgcctacctcaacttccataaagaattactcaataccgctccttttctttctattcttcttcttccgtgcatatttgattatcagcttaatctttcggtgaaacaagtaaaatctgttcatgggtttgcgccaaaatccaatcttttggagcaagacttgctcgtattgttgaaccatgtagtccatgcctattcgaaatgctcggaatttggcacagctcgtaaggtgtttgatgaaatgtatgagagaaacatgttctcatggacgatcatgattgttgggtctacagagaatggtttgttgtttgatgggtttgagttgtttagggacatggtgaacaatggagttttacctgacaaatttgtatataccgcgattatccagacatgtattggtctagattgtgttgaattgggaaaaatgcatggtgcatgcatgctcgaattgttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

220

Amino Acids

25.06

Weight (kDa)

6.06

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 154 - 200 1.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 248
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 584
AciI CCGC 2 cut(s) 246, 582
AcsI RAATTY 2 cut(s) 416, 569
AcvI CACGTG 1 cut(s) 97
AflIII ACRYGT 2 cut(s) 462, 596
AgsI TTSAA 3 cut(s) 48, 382, 620
AluBI AGCT 4 cut(s) 122, 147, 295, 428
AluI AGCT 4 cut(s) 122, 147, 295, 428
Alw21I GWGCWC 2 cut(s) 96, 149
AlwNI CAGNNNCTG 1 cut(s) 27
ApeKI GCWGC 2 cut(s) 119, 162
ApoI RAATTY 2 cut(s) 416, 569
AspLEI GCGC 1 cut(s) 340
AsuHPI GGTGA 2 cut(s) 319, 556
AsuII TTCGAA 1 cut(s) 404
BanII GRGCYC 1 cut(s) 149
BbrPI CACGTG 1 cut(s) 97
Bbv12I GWGCWC 2 cut(s) 96, 149
BbvI GCAGC 2 cut(s) 131, 149
BccI CCATC 1 cut(s) 512
BceAI ACGGC 1 cut(s) 103
BfaI CTAG 2 cut(s) 102, 608
BfmI CTRYAG 1 cut(s) 495
BfuAI ACCTGC 1 cut(s) 200
BisI GCNGC 2 cut(s) 120, 163
BlsI GCNGC 2 cut(s) 121, 164
Bpu14I TTCGAA 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 97
BseXI GCAGC 2 cut(s) 131, 149
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 584
BsiHKAI GWGCWC 2 cut(s) 96, 149
BslFI GGGAC 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 551
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 96, 149
Bsp143I GATC 1 cut(s) 477
BspACI CCGC 2 cut(s) 246, 582
BspFNI CGCG 1 cut(s) 584
BspHI TCATGA 1 cut(s) 480
BspMI ACCTGC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 248
BssMI GATC 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 97
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 92, 643, 647
BstFNI CGCG 1 cut(s) 584
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 1 cut(s) 480
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNSI RCATGY 4 cut(s) 466, 600, 645, 649
BstSFI CTRYAG 1 cut(s) 495
BstUI CGCG 1 cut(s) 584
BstV1I GCAGC 2 cut(s) 131, 149
BstXI CCANNNNNNTGG 1 cut(s) 355
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 3 cut(s) 92, 643, 647
CaiI CAGNNNCTG 1 cut(s) 27
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 340
CviJI RGCY 5 cut(s) 122, 147, 181, 295, 428
CviKI_1 RGCY 5 cut(s) 122, 147, 181, 295, 428
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 147
Eco72I CACGTG 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 147
EcoT22I ATGCAT 2 cut(s) 636, 647
EcoT38I GRGCYC 1 cut(s) 149
FaqI GGGAC 1 cut(s) 551
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 120, 163
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 120, 163
FspBI CTAG 2 cut(s) 102, 608
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 120, 163
HhaI GCGC 1 cut(s) 340
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 1 cut(s) 24
HphI GGTGA 2 cut(s) 319, 556
Hpy166II GTNNAC 2 cut(s) 495, 547
Hpy188I TCNGA 4 cut(s) 35, 55, 144, 415
Hpy188III TCNNGA 3 cut(s) 481, 592, 608
Hpy8I GTNNAC 2 cut(s) 495, 547
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 5 cut(s) 165, 279, 634, 641, 645
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpySE526I ACGT 1 cut(s) 96
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 1 cut(s) 477
LmnI GCTCC 4 cut(s) 144, 152, 253, 358
LpnPI CCDG 5 cut(s) 34, 110, 205, 576, 605
Lsp1109I GCAGC 2 cut(s) 131, 149
MaeI CTAG 2 cut(s) 102, 608
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 97
MalI GATC 1 cut(s) 479
MbiI CCGCTC 1 cut(s) 248
MboI GATC 1 cut(s) 477
MboII GAAGA 3 cut(s) 257, 260, 263
MhlI GDGCHC 2 cut(s) 96, 149
MluCI AATT 7 cut(s) 10, 113, 233, 416, 569, 620, 653
MmeI TCCRAC 2 cut(s) 90, 122
MnlI CCTC 3 cut(s) 179, 196, 227
Mph1103I ATGCAT 2 cut(s) 636, 647
MseI TTAA 1 cut(s) 297
MvnI CGCG 1 cut(s) 584
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 97
NsiI ATGCAT 2 cut(s) 636, 647
NspI RCATGY 4 cut(s) 466, 600, 645, 649
NspV TTCGAA 1 cut(s) 404
PaeI GCATGC 2 cut(s) 645, 649
PagI TCATGA 1 cut(s) 480
PciI ACATGT 2 cut(s) 462, 596
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 24
PkrI GCNGC 2 cut(s) 121, 164
PmaCI CACGTG 1 cut(s) 97
PmlI CACGTG 1 cut(s) 97
Ppu21I YACGTR 1 cut(s) 97
PscI ACATGT 2 cut(s) 462, 596
Psp124BI GAGCTC 1 cut(s) 149
PspCI CACGTG 1 cut(s) 97
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
PstNI CAGNNNCTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 2 cut(s) 120, 163
Sau3AI GATC 1 cut(s) 477
SduI GDGCHC 2 cut(s) 96, 149
SfcI CTRYAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 404
SphI GCATGC 2 cut(s) 645, 649
Sse9I AATT 7 cut(s) 10, 113, 233, 416, 569, 620, 653
SsiI CCGC 2 cut(s) 246, 582
SspMI CTAG 2 cut(s) 102, 608
SstI GAGCTC 1 cut(s) 149
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 135, 404, 651
TasI AATT 7 cut(s) 10, 113, 233, 416, 569, 620, 653
TfiI GAWTC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 119, 162
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 3 cut(s) 69, 317, 459
TspGWI ACGGA 2 cut(s) 139, 264
XapI RAATTY 2 cut(s) 416, 569
XbaI TCTAGA 1 cut(s) 607
XceI RCATGY 4 cut(s) 466, 600, 645, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 2 cut(s) 102, 608
Zsp2I ATGCAT 2 cut(s) 636, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.