Rmu_sc0002676.1_g000004

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002676.1
N/A
Physical Location & Seq
Forward (+)
18890 .. 23210
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1614 bp
atgggcagcagcatgcagctctgctcagaaagccaccatgcattgttcagaggcccttgcaagaatagaattaacgttcagatatacggaaccttgattgccccgactgattattgggctctgggaaactctggctattggatcttatttattaaggtcaatagacttgcatttttcggtggtacactcgacgccaagggagctggcatttgggcttgcaggaaatccggaaagagcttccctgttggagctaggtcgataacgttcaattgggcaaatgacgttgtgattagtggtttagtatccatcaacagtcagcaaactcatcttgtgatcaacagctgcaacaatgtggtggtccgaaacgtaaagctggttgctccggatgacagccccaacactgatggcattcgtgtgcagagctcaactggtgttactgtcactggcgcaaccttgcaaaccggagacgattgtgaatccaacctctacatgagtaacatcaagtgcggcctggacatggcatatggtatattcttacgcattggtagtctaggcaacgcagtgaacgaagctggggtccaaaatgtgacgttgacgaatgcaattttcactggatcggacaatggagtacgaataaagtcatgggccaggcccagcaatagttttgtgattctggaagaacatgatgatgaggaacgagaaccccatcatcatcgatcagaactactgcccaaaaaacgaagagttctggtgcgacgtgtgatgattagtcaagtgacatacaagaatatacaagggacgtcggcaaaggcggaggcagtaacatttgattgcagcccaagtaatccgtgcagagggattagattgcaagacatcaagcttacatacatgaatccggtagccacatcttcatgcaagaacattcgtggaagcaccactggagtatactcatgccggtactccggtggaaatggaggagctaggagggtggtttgtgtaacctctggtggtaccacggttcctttggagcaacgatgtgtgcgctacattgacaacttcagctcaggggaacctgccaaccatattgcagatctcttcccgatgatccgttacttgaatgtctggaaattttggatgagtcaaacattcaagatgttgcagatggttgcgtttacaatacgaagtattcgatcacatgccatgctatatcttgcagcagcagtatctgacttttacgtcccttggaaaagcatggcagaacacaaaattcagtcaggctctggtccattggatatgcgacttgttcaggtgccaaagatgctcttagtgctgaggaaagaatgggcccccatggccttctgcatatcattcaagctagaaacagattcaaagatcctgttagagaaagctgatatggctcttaggaagtacaagatgcatatggttgtagccaatgagcttttgagccgaaaggaagaggttgtagttgtcaccagcaatgaaaagatttcagttcgccgaaacaagtctctgagtgttgatgatgtggagagccccctggttgagcttcttgtggagaagcattcagcctatgttgagaattctgatctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

537

Amino Acids

59.62

Weight (kDa)

9.2

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 15 - 216 5.4e-37 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 253 - 309 2.6e-09 Glycosyl hydrolases family 28
DFP PF04127 387 - 506 2.5e-10 DNA/pantothenate metabolism flavoprotein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1235
AatII GACGTC 1 cut(s) 803
Acc36I ACCTGC 1 cut(s) 1081
Acc65I GGTACC 1 cut(s) 1010
AccB1I GGYRCC 2 cut(s) 1010, 1309
AccI GTMKAC 1 cut(s) 945
AccIII TCCGGA 2 cut(s) 227, 382
AciI CCGC 2 cut(s) 507, 812
AclI AACGTT 2 cut(s) 75, 263
AclWI GGATC 4 cut(s) 149, 622, 1099, 1387
AcsI RAATTY 3 cut(s) 1127, 1266, 1600
AcuI CTGAAG 1 cut(s) 1042
AcyI GRCGYC 2 cut(s) 192, 800
AfaI GTAC 5 cut(s) 184, 630, 959, 1012, 1430
AfiI CCNNNNNNNGG 2 cut(s) 517, 854
AflIII ACRYGT 1 cut(s) 757
AgsI TTSAA 5 cut(s) 268, 1117, 1150, 1372, 1389
AjiI CACGTC 1 cut(s) 758
AjnI CCWGG 3 cut(s) 510, 647, 1557
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 2 cut(s) 459, 1533
AlwI GGATC 4 cut(s) 149, 622, 1099, 1387
AlwNI CAGNNNCTG 2 cut(s) 1226, 1280
Aor13HI TCCGGA 2 cut(s) 227, 382
AoxI GGCC 6 cut(s) 52, 508, 645, 650, 1344, 1353
ApaI GGGCCC 1 cut(s) 1348
ApeKI GCWGC 7 cut(s) 6, 9, 16, 342, 834, 1214, 1217
ApoI RAATTY 3 cut(s) 1127, 1266, 1600
ArsI GACNNNNNNTTYG 2 cut(s) 561, 593
Asp718I GGTACC 1 cut(s) 1010
AspLEI GCGC 2 cut(s) 449, 1044
AspS9I GGNCC 8 cut(s) 53, 358, 577, 645, 651, 1283, 1344, 1345
AsuHPI GGTGA 1 cut(s) 1483
AvaII GGWCC 3 cut(s) 358, 577, 1283
BaeGI GKGCMC 1 cut(s) 1348
BaeI ACNNNNGTAYC 4 cut(s) 949, 982, 1002, 1035
BanI GGYRCC 2 cut(s) 1010, 1309
BanII GRGCYC 4 cut(s) 121, 425, 1348, 1556
Bbv12I GWGCWC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 1331
BbvI GCAGC 7 cut(s) 18, 21, 28, 329, 846, 1226, 1229
BccI CCATC 4 cut(s) 314, 398, 714, 1156
BciT130I CCWGG 3 cut(s) 512, 649, 1559
BciVI GTATCC 1 cut(s) 313
BclI TGATCA 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 459, 1533
BfaI CTAG 4 cut(s) 252, 551, 981, 1376
BfuAI ACCTGC 1 cut(s) 1081
BfuI GTATCC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 1352
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 8 cut(s) 7, 10, 17, 343, 508, 835, 1215, 1218
BlsI GCNGC 8 cut(s) 8, 11, 18, 344, 509, 836, 1216, 1219
Bme1390I CCNGG 3 cut(s) 512, 649, 1559
Bme18I GGWCC 3 cut(s) 358, 577, 1283
BmgBI CACGTC 1 cut(s) 758
BmgT120I GGNCC 8 cut(s) 53, 358, 577, 645, 651, 1283, 1344, 1345
BmiI GGNNCC 8 cut(s) 91, 578, 1012, 1020, 1071, 1311, 1346, 1347
BmrFI CCNGG 3 cut(s) 512, 649, 1559
BmsI GCATC 2 cut(s) 1308, 1425
BpmI CTGGAG 1 cut(s) 960
Bpu10I CCTNAGC 2 cut(s) 1063, 1331
Bsa29I ATCGAT 1 cut(s) 715
BsaHI GRCGYC 2 cut(s) 192, 800
BsaJI CCNNGG 5 cut(s) 195, 1014, 1241, 1350, 1557
BsaWI WCCGGW 5 cut(s) 227, 382, 461, 895, 962
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 854
Bse118I RCCGGY 1 cut(s) 954
Bse1I ACTGG 4 cut(s) 433, 448, 616, 943
Bse3DI GCAATG 1 cut(s) 1504
BseAI TCCGGA 2 cut(s) 227, 382
BseBI CCWGG 3 cut(s) 512, 649, 1559
BseCI ATCGAT 1 cut(s) 715
BseDI CCNNGG 5 cut(s) 195, 1014, 1241, 1350, 1557
BseGI GGATG 2 cut(s) 391, 1140
BseLI CCNNNNNNNGG 2 cut(s) 517, 854
BseMI GCAATG 1 cut(s) 1504
BseMII CTCAG 4 cut(s) 39, 1077, 1322, 1523
BseNI ACTGG 4 cut(s) 433, 448, 616, 943
BseRI GAGGAG 1 cut(s) 990
BseSI GKGCMC 1 cut(s) 1348
BseXI GCAGC 7 cut(s) 18, 21, 28, 329, 846, 1226, 1229
BseYI CCCAGC 2 cut(s) 572, 653
BsgI GTGCAG 2 cut(s) 437, 871
BshFI GGCC 6 cut(s) 54, 510, 647, 652, 1346, 1355
BshNI GGYRCC 2 cut(s) 1010, 1309
BshVI ATCGAT 1 cut(s) 715
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 6 cut(s) 228, 383, 462, 896, 955, 963
BslFI GGGAC 2 cut(s) 811, 1223
BslI CCNNNNNNNGG 2 cut(s) 517, 854
BsmAI GTCTC 2 cut(s) 459, 1533
BsmBI CGTCTC 1 cut(s) 459
BsmFI GGGAC 2 cut(s) 811, 1223
BsmI GAATGC 3 cut(s) 408, 604, 1582
BsnI GGCC 6 cut(s) 54, 510, 647, 652, 1346, 1355
Bsp120I GGGCCC 1 cut(s) 1344
Bsp1286I GDGCHC 4 cut(s) 121, 425, 1348, 1556
Bsp13I TCCGGA 2 cut(s) 227, 382
Bsp143I GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
Bsp19I CCATGG 1 cut(s) 1350
BspACI CCGC 2 cut(s) 507, 812
BspANI GGCC 6 cut(s) 54, 510, 647, 652, 1346, 1355
BspCNI CTCAG 4 cut(s) 38, 1076, 1323, 1524
BspDI ATCGAT 1 cut(s) 715
BspEI TCCGGA 2 cut(s) 227, 382
BspLI GGNNCC 8 cut(s) 91, 578, 1012, 1020, 1071, 1311, 1346, 1347
BspMI ACCTGC 1 cut(s) 1081
BspPI GGATC 4 cut(s) 149, 622, 1099, 1387
BspT107I GGYRCC 2 cut(s) 1010, 1309
BsrDI GCAATG 1 cut(s) 1504
BsrFI RCCGGY 1 cut(s) 954
BsrI ACTGG 4 cut(s) 433, 448, 616, 943
BssAI RCCGGY 1 cut(s) 954
BssECI CCNNGG 5 cut(s) 195, 1014, 1241, 1350, 1557
BssMI GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
BssNAI GTATAC 1 cut(s) 946
BssNI GRCGYC 2 cut(s) 192, 800
BssT1I CCWWGG 3 cut(s) 195, 1241, 1350
Bst1107I GTATAC 1 cut(s) 946
Bst2UI CCWGG 3 cut(s) 512, 649, 1559
Bst4CI ACNGT 3 cut(s) 314, 439, 1018
Bst6I CTCTTC 3 cut(s) 736, 1100, 1470
BstACI GRCGYC 2 cut(s) 192, 800
BstC8I GCNNGC 3 cut(s) 14, 205, 217
BstDEI CTNAG 6 cut(s) 25, 1063, 1324, 1331, 1421, 1532
BstDSI CCRYGG 2 cut(s) 1014, 1350
BstF5I GGATG 2 cut(s) 391, 1140
BstHHI GCGC 2 cut(s) 449, 1044
BstKTI GATC 9 cut(s) 144, 336, 617, 719, 1093, 1107, 1193, 1395, 1609
BstMAI GTCTC 2 cut(s) 459, 1533
BstMBI GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
BstMWI GCNNNNNNNGC 6 cut(s) 30, 200, 1318, 1327, 1352, 1415
BstNI CCWGG 3 cut(s) 512, 649, 1559
BstNSI RCATGY 2 cut(s) 16, 1199
BstSCI CCNGG 3 cut(s) 510, 647, 1557
BstSLI GKGCMC 1 cut(s) 1348
BstV1I GCAGC 7 cut(s) 18, 21, 28, 329, 846, 1226, 1229
BstX2I RGATCY 3 cut(s) 141, 1090, 1392
BstYI RGATCY 3 cut(s) 141, 1090, 1392
BstZ17I GTATAC 1 cut(s) 946
Bsu15I ATCGAT 1 cut(s) 715
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 6 cut(s) 54, 510, 647, 652, 1346, 1355
BsuTUI ATCGAT 1 cut(s) 715
BtgI CCRYGG 2 cut(s) 1014, 1350
BtrI CACGTC 1 cut(s) 758
BtsCI GGATG 2 cut(s) 391, 1140
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 5 cut(s) 399, 441, 567, 609, 936
BveI ACCTGC 1 cut(s) 1081
Cac8I GCNNGC 3 cut(s) 14, 205, 217
CaiI CAGNNNCTG 2 cut(s) 1226, 1280
CfoI GCGC 2 cut(s) 449, 1044
Cfr10I RCCGGY 1 cut(s) 954
Cfr13I GGNCC 8 cut(s) 53, 358, 577, 645, 651, 1283, 1344, 1345
ClaI ATCGAT 1 cut(s) 715
CseI GACGC 1 cut(s) 200
Csp6I GTAC 5 cut(s) 183, 629, 958, 1011, 1429
CviQI GTAC 5 cut(s) 183, 629, 958, 1011, 1429
DdeI CTNAG 6 cut(s) 25, 1063, 1324, 1331, 1421, 1532
DpnI GATC 9 cut(s) 143, 335, 616, 718, 1092, 1106, 1192, 1394, 1608
DpnII GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
DrdI GACNNNNNNGTC 1 cut(s) 1235
DseDI GACNNNNNNGTC 1 cut(s) 1235
Eam1104I CTCTTC 3 cut(s) 736, 1100, 1470
EarI CTCTTC 3 cut(s) 736, 1100, 1470
EciI GGCGGA 1 cut(s) 827
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 3 cut(s) 195, 1241, 1350
Eco24I GRGCYC 4 cut(s) 121, 425, 1348, 1556
Eco47I GGWCC 3 cut(s) 358, 577, 1283
Eco53kI GAGCTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 1042
EcoICRI GAGCTC 1 cut(s) 423
EcoO109I RGGNCCY 2 cut(s) 53, 1345
EcoRI GAATTC 1 cut(s) 1600
EcoRII CCWGG 3 cut(s) 510, 647, 1557
EcoT14I CCWWGG 3 cut(s) 195, 1241, 1350
EcoT22I ATGCAT 2 cut(s) 43, 1440
EcoT38I GRGCYC 4 cut(s) 121, 425, 1348, 1556
ErhI CCWWGG 3 cut(s) 195, 1241, 1350
Esp3I CGTCTC 1 cut(s) 459
FalI AAGNNNNNCTT 2 cut(s) 1307, 1339
FaqI GGGAC 2 cut(s) 811, 1223
FauNDI CATATG 2 cut(s) 523, 1440
FbaI TGATCA 1 cut(s) 333
FblI GTMKAC 1 cut(s) 945
Fnu4HI GCNGC 8 cut(s) 7, 10, 17, 343, 508, 835, 1215, 1218
FokI GGATG 2 cut(s) 398, 1147
FriOI GRGCYC 4 cut(s) 121, 425, 1348, 1556
Fsp4HI GCNGC 8 cut(s) 7, 10, 17, 343, 508, 835, 1215, 1218
FspBI CTAG 4 cut(s) 252, 551, 981, 1376
GlaI GCGC 2 cut(s) 448, 1043
GluI GCNGC 8 cut(s) 7, 10, 17, 343, 508, 835, 1215, 1218
GsaI CCCAGC 2 cut(s) 576, 657
GsuI CTGGAG 1 cut(s) 960
HaeIII GGCC 6 cut(s) 54, 510, 647, 652, 1346, 1355
HapII CCGG 6 cut(s) 228, 383, 462, 896, 955, 963
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 449, 1044
Hin1I GRCGYC 2 cut(s) 192, 800
Hin6I GCGC 2 cut(s) 447, 1042
HinP1I GCGC 2 cut(s) 447, 1042
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 5 cut(s) 476, 670, 892, 1138, 1385
HpaII CCGG 6 cut(s) 228, 383, 462, 896, 955, 963
HphI GGTGA 1 cut(s) 1483
Hpy166II GTNNAC 5 cut(s) 185, 565, 594, 946, 1173
Hpy188I TCNGA 9 cut(s) 28, 50, 81, 362, 619, 721, 1228, 1533, 1606
Hpy188III TCNNGA 6 cut(s) 228, 383, 674, 1099, 1123, 1150
Hpy8I GTNNAC 5 cut(s) 185, 565, 594, 946, 1173
Hpy99I CGWCG 3 cut(s) 194, 759, 805
HpyAV CCTTC 1 cut(s) 1366
HpyCH4III ACNGT 3 cut(s) 314, 439, 1018
HpyCH4IV ACGT 8 cut(s) 75, 263, 282, 366, 590, 757, 800, 1236
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 200, 1318, 1327, 1352, 1415
HpyF3I CTNAG 6 cut(s) 25, 1063, 1324, 1331, 1421, 1532
HpySE526I ACGT 8 cut(s) 75, 263, 282, 366, 590, 757, 800, 1236
Hsp92I GRCGYC 2 cut(s) 192, 800
HspAI GCGC 2 cut(s) 447, 1042
Kpn2I TCCGGA 2 cut(s) 227, 382
KpnI GGTACC 1 cut(s) 1014
Ksp22I TGATCA 1 cut(s) 333
Kzo9I GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
LmnI GCTCC 5 cut(s) 200, 248, 385, 977, 1027
Lsp1109I GCAGC 7 cut(s) 18, 21, 28, 329, 846, 1226, 1229
LweI GCATC 2 cut(s) 1308, 1425
MaeI CTAG 4 cut(s) 252, 551, 981, 1376
MaeII ACGT 8 cut(s) 75, 263, 282, 366, 590, 757, 800, 1236
MaeIII GTNAC 9 cut(s) 433, 439, 494, 586, 775, 820, 997, 1109, 1489
MalI GATC 9 cut(s) 143, 335, 616, 718, 1092, 1106, 1192, 1394, 1608
MboI GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
MboII GAAGA 5 cut(s) 689, 753, 900, 1087, 1487
MfeI CAATTG 1 cut(s) 268
MflI RGATCY 3 cut(s) 141, 1090, 1392
MhlI GDGCHC 4 cut(s) 121, 425, 1348, 1556
MluCI AATT 6 cut(s) 69, 268, 603, 1127, 1266, 1600
MlyI GAGTC 1 cut(s) 1147
MmeI TCCRAC 2 cut(s) 226, 504
Mph1103I ATGCAT 2 cut(s) 43, 1440
MroI TCCGGA 2 cut(s) 227, 382
MseI TTAA 2 cut(s) 72, 153
MslI CAYNNNNRTG 3 cut(s) 413, 687, 910
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 6 cut(s) 228, 383, 462, 896, 955, 963
MspR9I CCNGG 3 cut(s) 512, 649, 1559
MunI CAATTG 1 cut(s) 268
Mva1269I GAATGC 3 cut(s) 408, 604, 1582
MvaI CCWGG 3 cut(s) 512, 649, 1559
MwoI GCNNNNNNNGC 6 cut(s) 30, 200, 1318, 1327, 1352, 1415
NcoI CCATGG 1 cut(s) 1350
NdeI CATATG 2 cut(s) 523, 1440
NdeII GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
NlaIV GGNNCC 8 cut(s) 91, 578, 1012, 1020, 1071, 1311, 1346, 1347
NmuCI GTSAC 4 cut(s) 439, 586, 775, 1489
NsiI ATGCAT 2 cut(s) 43, 1440
NspI RCATGY 2 cut(s) 16, 1199
PaeI GCATGC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 2 cut(s) 564, 1186
PctI GAATGC 3 cut(s) 408, 604, 1582
PfeI GAWTC 4 cut(s) 476, 670, 892, 1385
PkrI GCNGC 8 cut(s) 8, 11, 18, 344, 509, 836, 1216, 1219
PleI GAGTC 1 cut(s) 1146
PpsI GAGTC 1 cut(s) 1146
Psp124BI GAGCTC 1 cut(s) 425
Psp1406I AACGTT 2 cut(s) 75, 263
Psp6I CCWGG 3 cut(s) 510, 647, 1557
PspFI CCCAGC 2 cut(s) 572, 653
PspGI CCWGG 3 cut(s) 510, 647, 1557
PspN4I GGNNCC 8 cut(s) 91, 578, 1012, 1020, 1071, 1311, 1346, 1347
PspOMI GGGCCC 1 cut(s) 1344
PspPI GGNCC 8 cut(s) 53, 358, 577, 645, 651, 1283, 1344, 1345
PstNI CAGNNNCTG 2 cut(s) 1226, 1280
PsuI RGATCY 3 cut(s) 141, 1090, 1392
PvuII CAGCTG 1 cut(s) 342
RsaI GTAC 5 cut(s) 184, 630, 959, 1012, 1430
RsaNI GTAC 5 cut(s) 183, 629, 958, 1011, 1429
RseI CAYNNNNRTG 3 cut(s) 413, 687, 910
SacI GAGCTC 1 cut(s) 425
SaqAI TTAA 2 cut(s) 72, 153
SatI GCNGC 8 cut(s) 7, 10, 17, 343, 508, 835, 1215, 1218
Sau3AI GATC 9 cut(s) 141, 333, 614, 716, 1090, 1104, 1190, 1392, 1606
Sau96I GGNCC 8 cut(s) 53, 358, 577, 645, 651, 1283, 1344, 1345
SchI GAGTC 1 cut(s) 1147
ScrFI CCNGG 3 cut(s) 512, 649, 1559
SduI GDGCHC 4 cut(s) 121, 425, 1348, 1556
SfaNI GCATC 2 cut(s) 1308, 1425
SfiI GGCCNNNNNGGCC 1 cut(s) 1352
SinI GGWCC 3 cut(s) 358, 577, 1283
SmiMI CAYNNNNRTG 3 cut(s) 413, 687, 910
SphI GCATGC 1 cut(s) 16
Sse9I AATT 6 cut(s) 69, 268, 603, 1127, 1266, 1600
SsiI CCGC 2 cut(s) 507, 812
SspMI CTAG 4 cut(s) 252, 551, 981, 1376
SstI GAGCTC 1 cut(s) 425
StyD4I CCNGG 3 cut(s) 510, 647, 1557
StyI CCWWGG 3 cut(s) 195, 1241, 1350
TaaI ACNGT 3 cut(s) 314, 439, 1018
TaiI ACGT 8 cut(s) 78, 266, 285, 369, 593, 760, 803, 1239
TaqI TCGA 4 cut(s) 189, 257, 715, 1189
TasI AATT 6 cut(s) 69, 268, 603, 1127, 1266, 1600
TatI WGTACW 1 cut(s) 1428
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 4 cut(s) 476, 670, 892, 1385
Tru1I TTAA 2 cut(s) 72, 153
Tru9I TTAA 2 cut(s) 72, 153
TscAI CASTG 5 cut(s) 406, 448, 567, 616, 943
TseFI GTSAC 4 cut(s) 439, 586, 775, 1489
TseI GCWGC 7 cut(s) 6, 9, 16, 342, 834, 1214, 1217
Tsp45I GTSAC 4 cut(s) 439, 586, 775, 1489
TspDTI ATGAA 3 cut(s) 900, 905, 1515
TspGWI ACGGA 3 cut(s) 102, 837, 1097
TspRI CASTG 5 cut(s) 406, 448, 567, 616, 943
VpaK11BI GGWCC 3 cut(s) 358, 577, 1283
XapI RAATTY 3 cut(s) 1127, 1266, 1600
XceI RCATGY 2 cut(s) 16, 1199
XcmI CCANNNNNNNNNTGG 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 945
XspI CTAG 4 cut(s) 252, 551, 981, 1376
ZraI GACGTC 1 cut(s) 801
Zsp2I ATGCAT 2 cut(s) 43, 1440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.