Rmu_sc0002853.1_g000010

S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002853.1
N/A
Physical Location & Seq
Forward (+)
71717 .. 72001
285 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 285 bp
atggctggaatccgattgggccctgatgactcagagatatcgcagcagagccgagctgctccggccgatcttatctcagacgacgatcgctccgtcgccgcggactcgtggtccatcaagagcgactatgggagcactctcgacgacgaccaacgccacgctgatgctgctgaggatctctccgccgctaacctccgcgccccctccgattacaagttgcgtcttccactccctctctctttattgtactgtattacaatacatgtcttgcattgtttgttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_31

Protein Analysis

94

Amino Acids

10.22

Weight (kDa)

4.45

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 101, 198
AciI CCGC 5 cut(s) 99, 101, 183, 186, 196
AclWI GGATC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 63
AfaI GTAC 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 262
AhdI GACNNNNNGTC 1 cut(s) 109
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
Alw21I GWGCWC 1 cut(s) 137
AlwI GGATC 1 cut(s) 183
AoxI GGCC 2 cut(s) 19, 63
ApaI GGGCCC 1 cut(s) 23
ApeKI GCWGC 3 cut(s) 43, 56, 167
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 3 cut(s) 19, 20, 111
AvaII GGWCC 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 23
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 137
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 43, 55, 154
BccI CCATC 1 cut(s) 122
BisI GCNGC 5 cut(s) 44, 57, 99, 168, 186
BlsI GCNGC 5 cut(s) 45, 58, 100, 169, 187
Bme18I GGWCC 1 cut(s) 111
BmeRI GACNNNNNGTC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 19, 20, 111
BmiI GGNNCC 1 cut(s) 21
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu10I CCTNAGC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
BseDI CCNNGG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 45, 90, 162
BseSI GKGCMC 1 cut(s) 23
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 3 cut(s) 43, 55, 154
Bsh1236I CGCG 2 cut(s) 101, 198
Bsh1285I CGRYCG 2 cut(s) 66, 88
BshFI GGCC 2 cut(s) 21, 65
BsiEI CGRYCG 2 cut(s) 66, 88
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 62
BsnI GGCC 2 cut(s) 21, 65
Bsp120I GGGCCC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 23, 137
Bsp143I GATC 3 cut(s) 67, 85, 175
BspACI CCGC 5 cut(s) 99, 101, 183, 186, 196
BspANI GGCC 2 cut(s) 21, 65
BspCNI CTCAG 3 cut(s) 44, 89, 163
BspFNI CGCG 2 cut(s) 101, 198
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 3 cut(s) 67, 85, 175
BssSI CACGAG 1 cut(s) 106
Bst2BI CACGAG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 251
BstDEI CTNAG 3 cut(s) 31, 76, 171
BstDSI CCRYGG 1 cut(s) 99
BstFNI CGCG 2 cut(s) 101, 198
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 3 cut(s) 70, 88, 178
BstMBI GATC 3 cut(s) 67, 85, 175
BstMCI CGRYCG 2 cut(s) 66, 88
BstMWI GCNNNNNNNGC 2 cut(s) 62, 167
BstNSI RCATGY 1 cut(s) 266
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 2 cut(s) 101, 198
BstV1I GCAGC 3 cut(s) 43, 55, 154
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 2 cut(s) 21, 65
BtgI CCRYGG 1 cut(s) 99
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 19, 20, 111
Cfr42I CCGCGG 1 cut(s) 102
CseI GACGC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 247
CviAII CATG 1 cut(s) 263
CviJI RGCY 5 cut(s) 5, 21, 51, 56, 65
CviKI_1 RGCY 5 cut(s) 5, 21, 51, 56, 65
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 3 cut(s) 31, 76, 171
DpnI GATC 3 cut(s) 69, 87, 177
DpnII GATC 3 cut(s) 67, 85, 175
DriI GACNNNNNGTC 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1105I GACNNNNNGTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 20
EcoRV GATATC 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 1 cut(s) 266
FaiI YATR 2 cut(s) 129, 264
FatI CATG 1 cut(s) 262
Fnu4HI GCNGC 5 cut(s) 44, 57, 99, 168, 186
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 44, 57, 99, 168, 186
GlaI GCGC 1 cut(s) 199
GluI GCNGC 5 cut(s) 44, 57, 99, 168, 186
HaeIII GGCC 2 cut(s) 21, 65
HapII CCGG 1 cut(s) 62
HgaI GACGC 1 cut(s) 209
HhaI GCGC 1 cut(s) 200
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 3 cut(s) 9, 29, 104
HpaII CCGG 1 cut(s) 62
Hpy188I TCNGA 4 cut(s) 14, 34, 79, 208
Hpy188III TCNNGA 2 cut(s) 118, 140
Hpy99I CGWCG 4 cut(s) 86, 98, 146, 149
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 1 cut(s) 271
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 167
HpyF3I CTNAG 3 cut(s) 31, 76, 171
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 102
Kzo9I GATC 3 cut(s) 67, 85, 175
LmnI GCTCC 3 cut(s) 64, 95, 132
LpnPI CCDG 2 cut(s) 36, 75
Lsp1109I GCAGC 3 cut(s) 43, 55, 154
LweI GCATC 1 cut(s) 154
MalI GATC 3 cut(s) 69, 87, 177
MboI GATC 3 cut(s) 67, 85, 175
MboII GAAGA 1 cut(s) 215
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 23, 137
MlyI GAGTC 2 cut(s) 23, 98
MnlI CCTC 4 cut(s) 166, 203, 214, 243
MslI CAYNNNNRTG 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 1 cut(s) 62
MvnI CGCG 2 cut(s) 101, 198
MwoI GCNNNNNNNGC 2 cut(s) 62, 167
NdeII GATC 3 cut(s) 67, 85, 175
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 21
NmeAIII GCCGAG 1 cut(s) 77
NspI RCATGY 1 cut(s) 266
PciI ACATGT 1 cut(s) 262
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 1 cut(s) 9
PkrI GCNGC 5 cut(s) 45, 58, 100, 169, 187
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 2 cut(s) 23, 98
PpsI GAGTC 2 cut(s) 23, 98
PscI ACATGT 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 21
PspOMI GGGCCC 1 cut(s) 19
PspPI GGNCC 3 cut(s) 19, 20, 111
PsuI RGATCY 1 cut(s) 175
PvuI CGATCG 1 cut(s) 88
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 162
SacII CCGCGG 1 cut(s) 102
SatI GCNGC 5 cut(s) 44, 57, 99, 168, 186
Sau3AI GATC 3 cut(s) 67, 85, 175
Sau96I GGNCC 3 cut(s) 19, 20, 111
SchI GAGTC 2 cut(s) 23, 98
SduI GDGCHC 2 cut(s) 23, 137
SetI ASST 2 cut(s) 58, 195
SfaNI GCATC 1 cut(s) 154
Sfr303I CCGCGG 1 cut(s) 102
SgrBI CCGCGG 1 cut(s) 102
SinI GGWCC 1 cut(s) 111
SmiMI CAYNNNNRTG 1 cut(s) 162
SsiI CCGC 5 cut(s) 99, 101, 183, 186, 196
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 141
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 101, 188
TfiI GAWTC 1 cut(s) 9
TseI GCWGC 3 cut(s) 43, 56, 167
TspGWI ACGGA 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 111
XceI RCATGY 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.