Rmu_sc0002923.1_g000035

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002923.1
N/A
Physical Location & Seq
Reverse (-)
151895 .. 157294
5400 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
atgatggacgctcatgagtggaagtactattggtcatcctgctgcgctcgcgcattggtcgtggccagagttgcagatactgctgaagctgcaaatggagcacaaaagagtttgggtatccagattaaagagcatgtggtcgggatgtctttgggctcaaatccaagcccagaccttggggagttcgatgaaggaggtggatgtgtggctaccctcgaagtggtcgacatggatgcttggccggacaacaccaatatagttgatggcggcaagaacaacttggaggcaactaggattcaaagggaaatgggggcaattccatgtctcatgctgaaaatgaatcttcaccttgaggaaagaattgaggcggctaagggcccgaggaaagaatcggggcggccagggcctgaggaaagactcggggcggccagggctcgaggaaagattcgggacggccagggctcgaggaaatattcaaggcaagatttgaagcagccagagaatggaaatttcctcttaggacgaatgtccaaagagaaaatttgggggcattgtgggagtgggaaaatttttcctcttaggacgagtgtccaaagagaaaatttgggggcattgtgggagtggtcaaattctgctaggcatgcaattaagacgattaactccgcatcgacggtcaaactccaagtcaacgctcaaaggaagcctctcgattgttcccggttcccaggtcagctcccgctccagtccgcattaattgttgggtcaaactcttctatgaaatttttcacctccaacaaccatcgttctggtattgattggaaattgaaatctgaagttttgtctcttgattttgttgggaaggatgtggctgttgcggatctggcaccaactcaccccataagattgggtctagctctgaacttctcgaaaatccaacggccgtccttcaccccatcccctccttgggccccaccaccccacctttatattccccccctccctcttctaccaactcaccccataagattgggtctagctctgaacttctcagtgttctactatgagattctcaatcagtctgacaaagcttgtagcatggccaaacaggcatttgaggaagccattgctgagctagacactttgggtgaagaatcttacaaggacagcactctcatcatgcaacttttgagggacaacctcaccctctggacttcagatgtgacggaagggttggatgaaccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

414

Amino Acids

45.69

Weight (kDa)

7.11

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 335 - 404 3.2e-34 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 883
AccB7I CCANNNNNTGG 2 cut(s) 176, 503
AccBSI CCGCTC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 51
AciI CCGC 8 cut(s) 267, 368, 397, 425, 663, 737, 747, 875
AclWI GGATC 1 cut(s) 885
AcoI YGGCCR 7 cut(s) 63, 239, 398, 426, 454, 938, 1098
AcsI RAATTY 6 cut(s) 508, 540, 567, 601, 628, 779
AcuI CTGAAG 3 cut(s) 105, 852, 1197
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 5 cut(s) 176, 220, 503, 963, 964
AgsI TTSAA 4 cut(s) 299, 477, 490, 826
AjnI CCWGG 4 cut(s) 400, 428, 456, 724
AluBI AGCT 6 cut(s) 89, 733, 914, 1037, 1088, 1132
AluI AGCT 6 cut(s) 89, 733, 914, 1037, 1088, 1132
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 2 cut(s) 329, 846
AlwI GGATC 1 cut(s) 885
AlwNI CAGNNNCTG 2 cut(s) 80, 407
Ama87I CYCGRG 4 cut(s) 379, 419, 435, 463
ApaI GGGCCC 2 cut(s) 380, 970
ApeKI GCWGC 3 cut(s) 42, 89, 493
ApoI RAATTY 6 cut(s) 508, 540, 567, 601, 628, 779
ArsI GACNNNNNNTTYG 2 cut(s) 669, 701
AseI ATTAAT 1 cut(s) 752
Asp700I GAANNNNTTC 1 cut(s) 782
AspLEI GCGC 2 cut(s) 47, 53
AspS9I GGNCC 5 cut(s) 376, 377, 404, 966, 967
AsuC2I CCSGG 1 cut(s) 718
AsuHPI GGTGA 7 cut(s) 338, 778, 884, 940, 1007, 1157, 1192
AvaI CYCGRG 4 cut(s) 379, 419, 435, 463
AxyI CCTNAGG 1 cut(s) 408
BaeGI GKGCMC 2 cut(s) 380, 970
BalI TGGCCA 2 cut(s) 65, 1100
BanI GGYRCC 1 cut(s) 883
BanII GRGCYC 5 cut(s) 158, 380, 436, 464, 970
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 3 cut(s) 29, 76, 505
BccI CCATC 3 cut(s) 257, 807, 961
BceAI ACGGC 3 cut(s) 469, 925, 953
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 4 cut(s) 402, 430, 458, 726
BciVI GTATCC 1 cut(s) 128
BcnI CCSGG 1 cut(s) 718
BcoDI GTCTC 2 cut(s) 329, 846
BfaI CTAG 5 cut(s) 291, 636, 911, 1034, 1133
BfuI GTATCC 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 1106
BisI GCNGC 7 cut(s) 43, 90, 268, 369, 398, 426, 494
BlpI GCTNAGC 1 cut(s) 1128
BlsI GCNGC 7 cut(s) 44, 91, 269, 370, 399, 427, 495
BmcAI AGTACT 1 cut(s) 26
Bme1390I CCNGG 5 cut(s) 402, 430, 458, 718, 726
BmeT110I CYCGRG 4 cut(s) 379, 419, 435, 463
BmgT120I GGNCC 5 cut(s) 376, 377, 404, 966, 967
BmiI GGNNCC 5 cut(s) 378, 722, 885, 968, 969
BmrFI CCNGG 5 cut(s) 402, 430, 458, 718, 726
BmsI GCATC 2 cut(s) 223, 674
BoxI GACNNNNGTC 2 cut(s) 526, 587
BpmI CTGGAG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 718
BsaJI CCNNGG 7 cut(s) 175, 380, 401, 429, 457, 724, 962
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 220, 503, 963, 964
Bse1I ACTGG 1 cut(s) 742
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 1122
BseBI CCWGG 4 cut(s) 402, 430, 458, 726
BseDI CCNNGG 7 cut(s) 175, 380, 401, 429, 457, 724, 962
BseGI GGATG 7 cut(s) 35, 150, 206, 238, 868, 953, 1240
BseLI CCNNNNNNNGG 5 cut(s) 176, 220, 503, 963, 964
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 3 cut(s) 399, 1062, 1119
BseNI ACTGG 1 cut(s) 742
BseSI GKGCMC 2 cut(s) 380, 970
BseX3I CGGCCG 1 cut(s) 938
BseXI GCAGC 3 cut(s) 29, 76, 505
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 941
BshNI GGYRCC 1 cut(s) 883
BsiEI CGRYCG 1 cut(s) 941
BsiHKAI GWGCWC 1 cut(s) 103
BsiHKCI CYCGRG 4 cut(s) 379, 419, 435, 463
BsiSI CCGG 2 cut(s) 242, 718
BslFI GGGAC 2 cut(s) 464, 1205
BslI CCNNNNNNNGG 5 cut(s) 176, 220, 503, 963, 964
BsmAI GTCTC 2 cut(s) 329, 846
BsmFI GGGAC 2 cut(s) 464, 1205
BsoBI CYCGRG 4 cut(s) 379, 419, 435, 463
Bsp120I GGGCCC 2 cut(s) 376, 966
Bsp1286I GDGCHC 6 cut(s) 103, 158, 380, 436, 464, 970
Bsp143I GATC 1 cut(s) 877
Bsp1720I GCTNAGC 1 cut(s) 1128
BspACI CCGC 8 cut(s) 267, 368, 397, 425, 663, 737, 747, 875
BspCNI CTCAG 3 cut(s) 400, 1061, 1120
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 5 cut(s) 378, 722, 885, 968, 969
BspPI GGATC 1 cut(s) 885
BspT107I GGYRCC 1 cut(s) 883
BsrBI CCGCTC 1 cut(s) 739
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 1 cut(s) 742
BssECI CCNNGG 7 cut(s) 175, 380, 401, 429, 457, 724, 962
BssMI GATC 1 cut(s) 877
BssT1I CCWWGG 2 cut(s) 175, 962
Bst2UI CCWGG 4 cut(s) 402, 430, 458, 726
Bst4CI ACNGT 1 cut(s) 673
Bst6I CTCTTC 2 cut(s) 775, 1008
BstAPI GCANNNNNTGC 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 49, 642
BstDEI CTNAG 6 cut(s) 372, 408, 517, 578, 1048, 1128
BstF5I GGATG 7 cut(s) 35, 150, 206, 238, 868, 953, 1240
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 2 cut(s) 47, 53
BstKTI GATC 1 cut(s) 880
BstMAI GTCTC 2 cut(s) 329, 846
BstMBI GATC 1 cut(s) 877
BstMCI CGRYCG 1 cut(s) 941
BstNI CCWGG 4 cut(s) 402, 430, 458, 726
BstNSI RCATGY 2 cut(s) 137, 644
BstPAI GACNNNNGTC 2 cut(s) 526, 587
BstSCI CCNGG 5 cut(s) 400, 428, 456, 716, 724
BstSLI GKGCMC 2 cut(s) 380, 970
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 3 cut(s) 29, 76, 505
BstX2I RGATCY 1 cut(s) 877
BstXI CCANNNNNNTGG 3 cut(s) 806, 904, 1027
BstYI RGATCY 1 cut(s) 877
BstZI CGGCCG 1 cut(s) 938
Bsu36I CCTNAGG 1 cut(s) 408
BsuI GTATCC 1 cut(s) 128
BtsCI GGATG 7 cut(s) 35, 150, 206, 238, 868, 953, 1240
BtsIMutI CAGTG 1 cut(s) 1056
Cac8I GCNNGC 2 cut(s) 49, 642
CaiI CAGNNNCTG 2 cut(s) 80, 407
CciI TCATGA 1 cut(s) 13
CfoI GCGC 2 cut(s) 47, 53
Cfr13I GGNCC 5 cut(s) 376, 377, 404, 966, 967
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 25
CviAII CATG 8 cut(s) 14, 134, 229, 321, 328, 641, 1096, 1177
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 6 cut(s) 372, 408, 517, 578, 1048, 1128
DpnI GATC 1 cut(s) 879
DpnII GATC 1 cut(s) 877
EaeI YGGCCR 7 cut(s) 63, 239, 398, 426, 454, 938, 1098
EagI CGGCCG 1 cut(s) 938
Eam1104I CTCTTC 2 cut(s) 775, 1008
EarI CTCTTC 2 cut(s) 775, 1008
EclXI CGGCCG 1 cut(s) 938
Eco130I CCWWGG 2 cut(s) 175, 962
Eco24I GRGCYC 5 cut(s) 158, 380, 436, 464, 970
Eco52I CGGCCG 1 cut(s) 938
Eco57I CTGAAG 3 cut(s) 105, 852, 1197
Eco81I CCTNAGG 1 cut(s) 408
Eco88I CYCGRG 4 cut(s) 379, 419, 435, 463
EcoO109I RGGNCCY 3 cut(s) 376, 404, 967
EcoRII CCWGG 4 cut(s) 400, 428, 456, 724
EcoT14I CCWWGG 2 cut(s) 175, 962
EcoT38I GRGCYC 5 cut(s) 158, 380, 436, 464, 970
ErhI CCWWGG 2 cut(s) 175, 962
FaeI CATG 8 cut(s) 17, 137, 232, 324, 331, 644, 1099, 1180
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 464, 1205
FatI CATG 8 cut(s) 13, 133, 228, 320, 327, 640, 1095, 1176
FauI CCCGC 1 cut(s) 744
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 7 cut(s) 43, 90, 268, 369, 398, 426, 494
FokI GGATG 6 cut(s) 22, 157, 213, 245, 875, 940
FriOI GRGCYC 5 cut(s) 158, 380, 436, 464, 970
Fsp4HI GCNGC 7 cut(s) 43, 90, 268, 369, 398, 426, 494
FspBI CTAG 5 cut(s) 291, 636, 911, 1034, 1133
GlaI GCGC 2 cut(s) 46, 52
GluI GCNGC 7 cut(s) 43, 90, 268, 369, 398, 426, 494
GsuI CTGGAG 1 cut(s) 725
HapII CCGG 2 cut(s) 242, 718
HgaI GACGC 1 cut(s) 17
HhaI GCGC 2 cut(s) 47, 53
Hin1II CATG 8 cut(s) 17, 137, 232, 324, 331, 644, 1099, 1180
Hin6I GCGC 2 cut(s) 45, 51
HinP1I GCGC 2 cut(s) 45, 51
HincII GTYRAC 2 cut(s) 226, 688
HindII GTYRAC 2 cut(s) 226, 688
HindIII AAGCTT 1 cut(s) 1086
HinfI GANTC 7 cut(s) 295, 340, 389, 417, 445, 1066, 1151
HpaII CCGG 2 cut(s) 242, 718
HphI GGTGA 7 cut(s) 338, 778, 884, 940, 1007, 1157, 1192
Hpy166II GTNNAC 2 cut(s) 226, 688
Hpy188I TCNGA 5 cut(s) 832, 918, 1041, 1081, 1216
Hpy188III TCNNGA 8 cut(s) 14, 121, 142, 449, 707, 845, 925, 1207
Hpy8I GTNNAC 2 cut(s) 226, 688
Hpy99I CGWCG 1 cut(s) 673
HpyAV CCTTC 4 cut(s) 185, 853, 955, 1220
HpyCH4III ACNGT 1 cut(s) 673
HpyCH4V TGCA 4 cut(s) 74, 92, 644, 1180
HpyF3I CTNAG 6 cut(s) 372, 408, 517, 578, 1048, 1128
Hsp92II CATG 8 cut(s) 17, 137, 232, 324, 331, 644, 1099, 1180
HspAI GCGC 2 cut(s) 45, 51
Kzo9I GATC 1 cut(s) 877
LmnI GCTCC 3 cut(s) 98, 738, 744
Lsp1109I GCAGC 3 cut(s) 29, 76, 505
LweI GCATC 2 cut(s) 223, 674
MaeI CTAG 5 cut(s) 291, 636, 911, 1034, 1133
MaeIII GTNAC 1 cut(s) 1219
MalI GATC 1 cut(s) 879
MbiI CCGCTC 1 cut(s) 739
MboI GATC 1 cut(s) 877
MboII GAAGA 4 cut(s) 335, 762, 995, 1160
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 6 cut(s) 103, 158, 380, 436, 464, 970
MlsI TGGCCA 2 cut(s) 65, 1100
MluNI TGGCCA 2 cut(s) 65, 1100
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 3 cut(s) 816, 958, 1212
Mox20I TGGCCA 2 cut(s) 65, 1100
MroXI GAANNNNTTC 1 cut(s) 782
MscI TGGCCA 2 cut(s) 65, 1100
MseI TTAA 4 cut(s) 126, 648, 657, 752
Msp20I TGGCCA 2 cut(s) 65, 1100
MspI CCGG 2 cut(s) 242, 718
MspR9I CCNGG 5 cut(s) 402, 430, 458, 718, 726
MvaI CCWGG 4 cut(s) 402, 430, 458, 726
MvnI CGCG 1 cut(s) 51
NciI CCSGG 1 cut(s) 718
NdeII GATC 1 cut(s) 877
NlaIII CATG 8 cut(s) 17, 137, 232, 324, 331, 644, 1099, 1180
NlaIV GGNNCC 5 cut(s) 378, 722, 885, 968, 969
NmuCI GTSAC 1 cut(s) 1219
NspI RCATGY 2 cut(s) 137, 644
PaeI GCATGC 1 cut(s) 644
PaeR7I CTCGAG 2 cut(s) 435, 463
PagI TCATGA 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 6 cut(s) 295, 340, 389, 445, 1066, 1151
PflMI CCANNNNNTGG 2 cut(s) 176, 503
PkrI GCNGC 7 cut(s) 44, 91, 269, 370, 399, 427, 495
PleI GAGTC 1 cut(s) 411
PpsI GAGTC 1 cut(s) 411
PshAI GACNNNNGTC 2 cut(s) 526, 587
PshBI ATTAAT 1 cut(s) 752
Psp6I CCWGG 4 cut(s) 400, 428, 456, 724
PspGI CCWGG 4 cut(s) 400, 428, 456, 724
PspN4I GGNNCC 5 cut(s) 378, 722, 885, 968, 969
PspOMI GGGCCC 2 cut(s) 376, 966
PspPI GGNCC 5 cut(s) 376, 377, 404, 966, 967
PspXI VCTCGAGB 2 cut(s) 435, 463
PstNI CAGNNNCTG 2 cut(s) 80, 407
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 4 cut(s) 126, 648, 657, 752
SatI GCNGC 7 cut(s) 43, 90, 268, 369, 398, 426, 494
Sau3AI GATC 1 cut(s) 877
Sau96I GGNCC 5 cut(s) 376, 377, 404, 966, 967
ScaI AGTACT 1 cut(s) 26
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 5 cut(s) 402, 430, 458, 718, 726
SduI GDGCHC 6 cut(s) 103, 158, 380, 436, 464, 970
SfaNI GCATC 2 cut(s) 223, 674
Sfr274I CTCGAG 2 cut(s) 435, 463
SlaI CTCGAG 2 cut(s) 435, 463
SmlI CTYRAG 3 cut(s) 350, 435, 463
SmoI CTYRAG 3 cut(s) 350, 435, 463
SphI GCATGC 1 cut(s) 644
SsiI CCGC 8 cut(s) 267, 368, 397, 425, 663, 737, 747, 875
SspI AATATT 1 cut(s) 473
SspMI CTAG 5 cut(s) 291, 636, 911, 1034, 1133
StyD4I CCNGG 5 cut(s) 400, 428, 456, 716, 724
StyI CCWWGG 2 cut(s) 175, 962
TaaI ACNGT 1 cut(s) 673
TaqI TCGA 8 cut(s) 186, 216, 225, 436, 464, 668, 708, 926
TatI WGTACW 1 cut(s) 24
TauI GCSGC 4 cut(s) 270, 371, 400, 428
TfiI GAWTC 6 cut(s) 295, 340, 389, 445, 1066, 1151
Tru1I TTAA 4 cut(s) 126, 648, 657, 752
Tru9I TTAA 4 cut(s) 126, 648, 657, 752
TscAI CASTG 1 cut(s) 1056
TseFI GTSAC 1 cut(s) 1219
TseI GCWGC 3 cut(s) 42, 89, 493
Tsp45I GTSAC 1 cut(s) 1219
TspDTI ATGAA 3 cut(s) 204, 353, 791
TspGWI ACGGA 1 cut(s) 1238
TspRI CASTG 1 cut(s) 1056
Van91I CCANNNNNTGG 2 cut(s) 176, 503
VspI ATTAAT 1 cut(s) 752
XapI RAATTY 6 cut(s) 508, 540, 567, 601, 628, 779
XceI RCATGY 2 cut(s) 137, 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 960
XhoI CTCGAG 2 cut(s) 435, 463
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 5 cut(s) 291, 636, 911, 1034, 1133
ZrmI AGTACT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.