Rmu_sc0003031.1_g000005

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003031.1
N/A
Physical Location & Seq
Reverse (-)
21210 .. 21772
563 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
atgactgggattgagaccaacccagctgtcaatggccaagtgccctttgccaaacccaatgaggtagttgtccaaggcgggaataatggcattccccaaaacaacaacaacaatgctcttgccaggagagtccctacaaaagctcatgttaggcacatgcactcacggtctttgaccatgagttcactgatcgaaggtcatcagcttggctctggcttgttgggcaaggcacaaggattttacgtcgctagttccgaagatgggagtagccagaccattgctttaactgcaatgtttgagagtagtgggtatgctgatagcctcacattctttggggttcacaaatcgagtagctgcttcagagtaggccttattatatggcctttggaccctaagcccgcccggcccgtagggcctgcccgaccttttcttaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

145

Amino Acids

15.44

Weight (kDa)

9.8

Isoelectric Point (pI)

59.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 62 - 116 1.4e-06 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 78, 399
AcoI YGGCCR 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 261
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 4 cut(s) 26, 143, 205, 354
AluI AGCT 4 cut(s) 26, 143, 205, 354
Alw26I GTCTC 1 cut(s) 8
AoxI GGCC 5 cut(s) 34, 367, 380, 404, 413
ApeKI GCWGC 1 cut(s) 354
AspS9I GGNCC 3 cut(s) 388, 405, 413
AsuC2I CCSGG 1 cut(s) 403
AvaII GGWCC 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 45
BalI TGGCCA 1 cut(s) 36
BbvI GCAGC 1 cut(s) 341
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 249
BglI GCCNNNNNGGC 2 cut(s) 403, 412
BisI GCNGC 1 cut(s) 355
BlsI GCNGC 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 124, 403
Bme18I GGWCC 1 cut(s) 388
BmgT120I GGNCC 3 cut(s) 388, 405, 413
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 2 cut(s) 124, 403
BmrI ACTGGG 1 cut(s) 15
BmuI ACTGGG 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 2 cut(s) 276, 297
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMI GCAATG 2 cut(s) 276, 297
BseNI ACTGG 1 cut(s) 10
BseSI GKGCMC 1 cut(s) 45
BseXI GCAGC 1 cut(s) 341
BseYI CCCAGC 1 cut(s) 22
BshFI GGCC 5 cut(s) 36, 369, 382, 406, 415
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 5 cut(s) 36, 369, 382, 406, 415
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 2 cut(s) 78, 399
BspANI GGCC 5 cut(s) 36, 369, 382, 406, 415
BspLI GGNNCC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 2 cut(s) 276, 297
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 168
BstC8I GCNNGC 2 cut(s) 399, 417
BstDEI CTNAG 1 cut(s) 393
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 4 cut(s) 222, 287, 403, 412
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 2 cut(s) 122, 401
BstSLI GKGCMC 1 cut(s) 45
BstV1I GCAGC 1 cut(s) 341
BsuRI GGCC 5 cut(s) 36, 369, 382, 406, 415
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 399, 417
Cfr13I GGNCC 3 cut(s) 388, 405, 413
CviAII CATG 3 cut(s) 146, 157, 178
DdeI CTNAG 1 cut(s) 393
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 73
Eco147I AGGCCT 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 3 cut(s) 149, 160, 181
FaiI YATR 6 cut(s) 147, 158, 179, 312, 377, 379
FaqI GGGAC 1 cut(s) 116
FatI CATG 3 cut(s) 145, 156, 177
FauI CCCGC 2 cut(s) 71, 406
Fnu4HI GCNGC 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 355
FspBI CTAG 1 cut(s) 249
GluI GCNGC 1 cut(s) 355
GsaI CCCAGC 1 cut(s) 26
HaeIII GGCC 5 cut(s) 36, 369, 382, 406, 415
HapII CCGG 1 cut(s) 403
Hin1II CATG 3 cut(s) 149, 160, 181
HinfI GANTC 1 cut(s) 129
HpaII CCGG 1 cut(s) 403
Hpy166II GTNNAC 2 cut(s) 185, 340
Hpy188I TCNGA 2 cut(s) 256, 362
Hpy8I GTNNAC 2 cut(s) 185, 340
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 2 cut(s) 160, 290
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 287, 403, 412
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 149, 160, 181
Kzo9I GATC 1 cut(s) 189
LpnPI CCDG 7 cut(s) 36, 109, 136, 198, 284, 416, 429
Lsp1109I GCAGC 1 cut(s) 341
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 243
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 1 cut(s) 269
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 36
MlyI GAGTC 1 cut(s) 138
MnlI CCTC 2 cut(s) 55, 332
Mox20I TGGCCA 1 cut(s) 36
MscI TGGCCA 1 cut(s) 36
MseI TTAA 2 cut(s) 284, 432
Msp20I TGGCCA 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 124, 403
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 4 cut(s) 222, 287, 403, 412
NciI CCSGG 1 cut(s) 403
NdeII GATC 1 cut(s) 189
NlaIII CATG 3 cut(s) 149, 160, 181
NlaIV GGNNCC 1 cut(s) 390
NspI RCATGY 1 cut(s) 160
PceI AGGCCT 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 90
PkrI GCNGC 1 cut(s) 356
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 3 cut(s) 388, 405, 413
PvuII CAGCTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 284, 432
SatI GCNGC 1 cut(s) 355
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 3 cut(s) 388, 405, 413
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 124, 403
SduI GDGCHC 1 cut(s) 45
SetI ASST 8 cut(s) 28, 66, 145, 199, 207, 246, 356, 427
SfiI GGCCNNNNNGGCC 1 cut(s) 412
SinI GGWCC 1 cut(s) 388
SseBI AGGCCT 1 cut(s) 369
SsiI CCGC 2 cut(s) 78, 399
SspMI CTAG 1 cut(s) 249
StuI AGGCCT 1 cut(s) 369
StyD4I CCNGG 2 cut(s) 122, 401
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 168
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 192, 347
Tru1I TTAA 2 cut(s) 284, 432
Tru9I TTAA 2 cut(s) 284, 432
TscAI CASTG 1 cut(s) 192
TseI GCWGC 1 cut(s) 354
TspRI CASTG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 388
XceI RCATGY 1 cut(s) 160
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.