Rmu_sc0003069.1_g000044

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003069.1
N/A
Physical Location & Seq
Forward (+)
181484 .. 186267
4784 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
atgaaagttgtagaagacgttaaaccgagctcgtgcatatgtggtgctgctaccaaagtttggtggccattggagctggtggcggcggcgccgccactgtggacggcggctgcggctacttcggatttcggggtgaaatctggggcgggtcctgtcacttggggtggcgccgctggttctggactcctgggatcgagtgcttcaaggtgtgaggtggaggcaaaaaaggcttcaaggttttgtattcggattcaaggtttttggcttcttgaatcagggttcggctatttgttcaggaacttgaggttcttggagagtttcaaggccttttcccagatgagaagtgctggggagagcccgacccagtttgcattagcaagtgtcatcaaagcttgtgtggttcttgggtcggttgagactgcgaggcagctgcattctcttgctttgaaattgggcttggcttgtgagctgtttgtgggtagtaacttggcggatatgtattcgaaatgtgggttcatggttgatgcttgtaaggcttgtcagtttggaaagtgtgttcatttgacggttgtgaagttaggattggaagtagaggttgctgtgggaaatgctctgattgatatgtactcaaaatcaggtgatatggagagtgctttaaatgtgtttcgggttgaccctgagtgcagaaatattgtgtcggcgggagttgagcctaatcagttcaatttttctaacttagtcaaggctggtgccagccaagctgcacttgaacaggggattcaacttcatgctcagtcttgcatgattcatggagataaagagaggggcaaactggcagcggagaaattgatacagcttgaacctgagaatagtggagcacatgttttgctttcaactctacatgcaacggagcaaaaatgggaagatttcagaagtttgtggaagatgatgagagacagcagcatgaagaaattgcctggttatagttgggttgatgttggaaacaaaacacacacgtttggagccgaagattggtctcatcctcagaagaaagaaatatatgagaaacttgatagtcttcttgatcagatcaagaaagctggatatgttccaaatacagatcttgttcttcatgatatggacgacaattcaaaggtaggcttcttcaccatcatagtgagaggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

395

Amino Acids

42.96

Weight (kDa)

7.08

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 270 - 332 2.2e-15 E motif
Eplus_motif PF20430 337 - 364 7.7e-10 E+ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 88, 167, 749
AccB7I CCANNNNNTGG 1 cut(s) 60
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 65
AcyI GRCGYC 2 cut(s) 89, 168
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 2 cut(s) 60, 1032
AflIII ACRYGT 2 cut(s) 880, 1014
AjnI CCWGG 2 cut(s) 186, 976
AjuI GAANNNNNNNTTGG 4 cut(s) 440, 472, 566, 598
AloI GAACNNNNNNTCC 2 cut(s) 540, 572
AluBI AGCT 8 cut(s) 30, 76, 392, 430, 469, 761, 856, 1100
AluI AGCT 8 cut(s) 30, 76, 392, 430, 469, 761, 856, 1100
Alw21I GWGCWC 2 cut(s) 32, 880
Alw26I GTCTC 3 cut(s) 410, 948, 1041
AlwI GGATC 1 cut(s) 199
AoxI GGCC 2 cut(s) 65, 324
ApeKI GCWGC 7 cut(s) 47, 110, 427, 430, 761, 836, 960
AspLEI GCGC 2 cut(s) 91, 170
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 145, 650, 1159
AsuII TTCGAA 1 cut(s) 503
AvaII GGWCC 1 cut(s) 149
BalI TGGCCA 1 cut(s) 67
BanI GGYRCC 3 cut(s) 88, 167, 749
BanII GRGCYC 2 cut(s) 32, 359
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 21, 1070
Bbv12I GWGCWC 2 cut(s) 32, 880
BbvI GCAGC 7 cut(s) 34, 97, 417, 439, 748, 848, 972
BccI CCATC 1 cut(s) 1178
BceAI ACGGC 1 cut(s) 120
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BciT130I CCWGG 2 cut(s) 188, 978
BclI TGATCA 1 cut(s) 1084
BcoDI GTCTC 3 cut(s) 410, 948, 1041
BfoI RGCGCY 2 cut(s) 92, 171
BglII AGATCT 1 cut(s) 1120
Bme1390I CCNGG 2 cut(s) 188, 978
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 5 cut(s) 90, 150, 169, 751, 1024
BmrFI CCNGG 2 cut(s) 188, 978
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 2 cut(s) 21, 1070
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 322
BsaHI GRCGYC 2 cut(s) 89, 168
BsaI GGTCTC 1 cut(s) 1041
BsaJI CCNNGG 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 1032
Bse1I ACTGG 2 cut(s) 364, 837
BseBI CCWGG 2 cut(s) 188, 978
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 1 cut(s) 1039
BseLI CCNNNNNNNGG 2 cut(s) 60, 1032
BseMII CTCAG 4 cut(s) 669, 806, 855, 1058
BseNI ACTGG 2 cut(s) 364, 837
BseXI GCAGC 7 cut(s) 34, 97, 417, 439, 748, 848, 972
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 2 cut(s) 703, 747
BshFI GGCC 2 cut(s) 67, 326
BshNI GGYRCC 3 cut(s) 88, 167, 749
BsiHKAI GWGCWC 2 cut(s) 32, 880
BslI CCNNNNNNNGG 2 cut(s) 60, 1032
BsmAI GTCTC 3 cut(s) 410, 948, 1041
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 2 cut(s) 67, 326
Bso31I GGTCTC 1 cut(s) 1041
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 3 cut(s) 32, 359, 880
Bsp143I GATC 4 cut(s) 191, 1084, 1089, 1120
BspANI GGCC 2 cut(s) 67, 326
BspCNI CTCAG 4 cut(s) 670, 805, 856, 1057
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 5 cut(s) 90, 150, 169, 751, 1024
BspPI GGATC 1 cut(s) 199
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 3 cut(s) 88, 167, 749
BspTNI GGTCTC 1 cut(s) 1041
BsrI ACTGG 2 cut(s) 364, 837
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 4 cut(s) 191, 1084, 1089, 1120
BssNI GRCGYC 2 cut(s) 89, 168
BssSI CACGAG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 188, 978
Bst4CI ACNGT 2 cut(s) 99, 568
BstACI GRCGYC 2 cut(s) 89, 168
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 754
BstDEI CTNAG 5 cut(s) 678, 736, 792, 864, 1044
BstF5I GGATG 1 cut(s) 1039
BstH2I RGCGCY 2 cut(s) 92, 171
BstHHI GCGC 2 cut(s) 91, 170
BstKTI GATC 4 cut(s) 194, 1087, 1092, 1123
BstMAI GTCTC 3 cut(s) 410, 948, 1041
BstMBI GATC 4 cut(s) 191, 1084, 1089, 1120
BstMWI GCNNNNNNNGC 5 cut(s) 73, 113, 227, 533, 758
BstNI CCWGG 2 cut(s) 188, 978
BstNSI RCATGY 2 cut(s) 884, 905
BstSCI CCNGG 2 cut(s) 186, 976
BstV1I GCAGC 7 cut(s) 34, 97, 417, 439, 748, 848, 972
BstV2I GAAGAC 2 cut(s) 21, 1070
BstX2I RGATCY 1 cut(s) 1120
BstYI RGATCY 1 cut(s) 1120
BsuRI GGCC 2 cut(s) 67, 326
BtsCI GGATG 1 cut(s) 1039
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 754
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 2 cut(s) 91, 170
Cfr13I GGNCC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 625
CviAII CATG 8 cut(s) 517, 788, 802, 809, 881, 902, 964, 1133
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 5 cut(s) 678, 736, 792, 864, 1044
DinI GGCGCC 2 cut(s) 90, 169
DpnI GATC 4 cut(s) 193, 1086, 1091, 1122
DpnII GATC 4 cut(s) 191, 1084, 1089, 1120
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 1 cut(s) 65
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 2 cut(s) 32, 359
Eco31I GGTCTC 1 cut(s) 1041
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 186, 976
EcoT38I GRGCYC 2 cut(s) 32, 359
EgeI GGCGCC 2 cut(s) 90, 169
EheI GGCGCC 2 cut(s) 90, 169
FaeI CATG 8 cut(s) 520, 791, 805, 812, 884, 905, 967, 1136
FalI AAGNNNNNCTT 4 cut(s) 750, 782, 1145, 1177
FatI CATG 8 cut(s) 516, 787, 801, 808, 880, 901, 963, 1132
FauI CCCGC 2 cut(s) 139, 694
FauNDI CATATG 1 cut(s) 38
FbaI TGATCA 1 cut(s) 1084
FokI GGATG 1 cut(s) 1026
FriOI GRGCYC 2 cut(s) 32, 359
GlaI GCGC 2 cut(s) 90, 169
GsaI CCCAGC 1 cut(s) 351
HaeII RGCGCY 2 cut(s) 92, 171
HaeIII GGCC 2 cut(s) 67, 326
HhaI GCGC 2 cut(s) 91, 170
Hin1I GRCGYC 2 cut(s) 89, 168
Hin1II CATG 8 cut(s) 520, 791, 805, 812, 884, 905, 967, 1136
Hin6I GCGC 2 cut(s) 89, 168
HinP1I GCGC 2 cut(s) 89, 168
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 5 cut(s) 183, 250, 272, 778, 805
HphI GGTGA 3 cut(s) 145, 650, 1159
Hpy166II GTNNAC 2 cut(s) 102, 673
Hpy188I TCNGA 6 cut(s) 124, 249, 615, 932, 1047, 1089
Hpy188III TCNNGA 6 cut(s) 180, 269, 295, 1082, 1093, 1133
Hpy8I GTNNAC 2 cut(s) 102, 673
HpyCH4III ACNGT 2 cut(s) 99, 568
HpyCH4IV ACGT 2 cut(s) 18, 1016
HpyCH4V TGCA 7 cut(s) 36, 371, 433, 684, 764, 801, 905
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 113, 227, 533, 758
HpyF3I CTNAG 5 cut(s) 678, 736, 792, 864, 1044
HpySE526I ACGT 2 cut(s) 18, 1016
Hsp92I GRCGYC 2 cut(s) 89, 168
Hsp92II CATG 8 cut(s) 520, 791, 805, 812, 884, 905, 967, 1136
HspAI GCGC 2 cut(s) 89, 168
KasI GGCGCC 2 cut(s) 88, 167
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 4 cut(s) 191, 1084, 1089, 1120
LmnI GCTCC 4 cut(s) 73, 875, 910, 1022
Lsp1109I GCAGC 7 cut(s) 34, 97, 417, 439, 748, 848, 972
LweI GCATC 1 cut(s) 514
MaeII ACGT 2 cut(s) 18, 1016
MaeIII GTNAC 2 cut(s) 154, 482
MalI GATC 4 cut(s) 193, 1086, 1091, 1122
MboI GATC 4 cut(s) 191, 1084, 1089, 1120
MboII GAAGA 9 cut(s) 26, 935, 955, 979, 1040, 1060, 1070, 1121, 1156
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 3 cut(s) 32, 359, 880
MlsI TGGCCA 1 cut(s) 67
MluCI AATT 5 cut(s) 449, 724, 845, 971, 1147
MluNI TGGCCA 1 cut(s) 67
Mly113I GGCGCC 2 cut(s) 89, 168
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 979
MnlI CCTC 8 cut(s) 205, 211, 297, 417, 586, 816, 1053, 1175
Mox20I TGGCCA 1 cut(s) 67
MscI TGGCCA 1 cut(s) 67
MseI TTAA 2 cut(s) 21, 656
MslI CAYNNNNRTG 1 cut(s) 1175
Msp20I TGGCCA 1 cut(s) 67
MspA1I CMGCKG 3 cut(s) 173, 430, 839
MspR9I CCNGG 2 cut(s) 188, 978
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 2 cut(s) 188, 978
MwoI GCNNNNNNNGC 5 cut(s) 73, 113, 227, 533, 758
NarI GGCGCC 2 cut(s) 89, 168
NdeI CATATG 1 cut(s) 38
NdeII GATC 4 cut(s) 191, 1084, 1089, 1120
NlaIII CATG 8 cut(s) 520, 791, 805, 812, 884, 905, 967, 1136
NlaIV GGNNCC 5 cut(s) 90, 150, 169, 751, 1024
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 2 cut(s) 884, 905
NspV TTCGAA 1 cut(s) 503
PagI TCATGA 1 cut(s) 1132
PceI AGGCCT 1 cut(s) 326
PciI ACATGT 1 cut(s) 880
PctI GAATGC 1 cut(s) 433
PfeI GAWTC 4 cut(s) 250, 272, 778, 805
PflMI CCANNNNNTGG 1 cut(s) 60
PleI GAGTC 1 cut(s) 177
PluTI GGCGCC 2 cut(s) 92, 171
PpsI GAGTC 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 149
PscI ACATGT 1 cut(s) 880
Psp124BI GAGCTC 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 186, 976
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 2 cut(s) 186, 976
PspN4I GGNNCC 5 cut(s) 90, 150, 169, 751, 1024
PspPI GGNCC 1 cut(s) 149
PspPPI RGGWCCY 1 cut(s) 149
PsuI RGATCY 1 cut(s) 1120
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 1175
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 21, 656
Sau3AI GATC 4 cut(s) 191, 1084, 1089, 1120
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 2 cut(s) 188, 978
SduI GDGCHC 3 cut(s) 32, 359, 880
SfaNI GCATC 1 cut(s) 514
SfoI GGCGCC 2 cut(s) 90, 169
SfuI TTCGAA 1 cut(s) 503
SinI GGWCC 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 1175
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 5 cut(s) 449, 724, 845, 971, 1147
SseBI AGGCCT 1 cut(s) 326
SspDI GGCGCC 2 cut(s) 88, 167
SspI AATATT 1 cut(s) 691
SstI GAGCTC 1 cut(s) 32
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 186, 976
TaaI ACNGT 2 cut(s) 99, 568
TaiI ACGT 2 cut(s) 21, 1019
TaqI TCGA 2 cut(s) 194, 503
TasI AATT 5 cut(s) 449, 724, 845, 971, 1147
TatI WGTACW 1 cut(s) 624
TauI GCSGC 6 cut(s) 86, 89, 94, 110, 116, 173
TfiI GAWTC 4 cut(s) 250, 272, 778, 805
Tru1I TTAA 2 cut(s) 21, 656
Tru9I TTAA 2 cut(s) 21, 656
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 7 cut(s) 47, 110, 427, 430, 761, 836, 960
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 7 cut(s) 17, 505, 548, 776, 797, 980, 1121
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 149
XceI RCATGY 2 cut(s) 884, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.