Rmu_sc0003113.1_g000012

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003113.1
N/A
Physical Location & Seq
Forward (+)
63663 .. 64358
696 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
atgtcgaacaaccagcatgaatatcgtgccgaagttgagttgaatatagttgcaccaacacggcttgtaccaacacggtccctattcacgtcatttccaccactcgatgacctttgctatgcggccagcatcggggaaaactgggatcgcgctcggttcatatttaatctccacaaacgacgtggtgatcttgctggccgaatatttgagctcgataccgaaccaccaccagtcctgatgttttataacgataaaccaccaccagtcatgacgttttataaagatgaacctagtaggttttcaaggagcaggcaattacaggacctgatcactgacgctcttagcaccatgggtgttccacaagctaggcagccacgtcttgcgaaaaaaatatatgaacggcgtacttatgatcatatcgacgaaatagacctaagagaatttcgacttggatttactgacgattatgttcttgtttacaatgttgacatatatgacatcgagattatggaatctgcatcactttaccatttgaaacttgtgactgacgtgcctgctggcagttcatgtcctatttgtttaagggagtgtgattttgaggtttctcgcttgccatgtctgcacatttttcataggtactgcctcaatcggtggttcgacatagaatggtcgtgtcctacctgcaaaatatgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

231

Amino Acids

27.14

Weight (kDa)

5.56

Isoelectric Point (pI)

71.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 189 - 229 4.2e-09 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 246, 279
Acc36I ACCTGC 1 cut(s) 689
AccII CGCG 1 cut(s) 150
AciI CCGC 1 cut(s) 122
AclWI GGATC 1 cut(s) 153
AcoI YGGCCR 2 cut(s) 123, 196
AcsI RAATTY 1 cut(s) 440
AfaI GTAC 3 cut(s) 69, 406, 638
AfiI CCNNNNNNNGG 1 cut(s) 132
AgsI TTSAA 3 cut(s) 43, 303, 535
AjiI CACGTC 4 cut(s) 90, 182, 377, 550
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 2 cut(s) 211, 365
AluI AGCT 2 cut(s) 211, 365
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 1 cut(s) 153
AlwNI CAGNNNCTG 1 cut(s) 325
AoxI GGCC 2 cut(s) 123, 196
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 1 cut(s) 440
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 78, 322
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 78, 322
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 77, 416
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BclI TGATCA 2 cut(s) 327, 412
BfaI CTAG 2 cut(s) 291, 366
BfuAI ACCTGC 1 cut(s) 689
BisI GCNGC 2 cut(s) 123, 371
BlsI GCNGC 2 cut(s) 124, 372
Bme18I GGWCC 2 cut(s) 78, 322
BmgBI CACGTC 4 cut(s) 90, 182, 377, 550
BmgT120I GGNCC 2 cut(s) 78, 322
BmiI GGNNCC 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 138, 527
BmuI ACTGGG 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 3 cut(s) 146, 230, 263
BseDI CCNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 132
BseNI ACTGG 3 cut(s) 146, 230, 263
BseXI GCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 2 cut(s) 125, 198
BsiHKAI GWGCWC 1 cut(s) 213
BslFI GGGAC 1 cut(s) 64
BslI CCNNNNNNNGG 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 2 cut(s) 125, 198
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 4 cut(s) 145, 187, 327, 412
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 1 cut(s) 122
BspANI GGCC 2 cut(s) 125, 198
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 80
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 1 cut(s) 153
BsrI ACTGG 3 cut(s) 146, 230, 263
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 4 cut(s) 145, 187, 327, 412
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 78
BstC8I GCNNGC 6 cut(s) 127, 196, 311, 555, 559, 611
BstDEI CTNAG 2 cut(s) 341, 434
BstDSI CCRYGG 1 cut(s) 348
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 4 cut(s) 148, 190, 330, 415
BstMBI GATC 4 cut(s) 145, 187, 327, 412
BstMWI GCNNNNNNNGC 1 cut(s) 619
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 125, 198
BtgI CCRYGG 1 cut(s) 348
BtrI CACGTC 4 cut(s) 90, 182, 377, 550
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 689
Cac8I GCNNGC 6 cut(s) 127, 196, 311, 555, 559, 611
CaiI CAGNNNCTG 1 cut(s) 325
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 78, 322
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 68, 405, 637
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 5 cut(s) 17, 268, 349, 567, 615
CviJI RGCY 6 cut(s) 64, 125, 198, 211, 365, 373
CviKI_1 RGCY 6 cut(s) 64, 125, 198, 211, 365, 373
CviQI GTAC 3 cut(s) 68, 405, 637
DdeI CTNAG 2 cut(s) 341, 434
DpnI GATC 4 cut(s) 147, 189, 329, 414
DpnII GATC 4 cut(s) 145, 187, 327, 412
EaeI YGGCCR 2 cut(s) 123, 196
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 78, 322
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 5 cut(s) 20, 271, 352, 570, 618
FaqI GGGAC 1 cut(s) 64
FatI CATG 5 cut(s) 16, 267, 348, 566, 614
FbaI TGATCA 2 cut(s) 327, 412
Fnu4HI GCNGC 2 cut(s) 123, 371
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 2 cut(s) 123, 371
FspBI CTAG 2 cut(s) 291, 366
GlaI GCGC 1 cut(s) 151
GluI GCNGC 2 cut(s) 123, 371
HaeIII GGCC 2 cut(s) 125, 198
HgaI GACGC 1 cut(s) 344
HhaI GCGC 1 cut(s) 152
Hin1II CATG 5 cut(s) 20, 271, 352, 570, 618
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 1 cut(s) 512
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 2 cut(s) 478, 487
Hpy188III TCNNGA 3 cut(s) 235, 268, 502
Hpy8I GTNNAC 2 cut(s) 478, 487
Hpy99I CGWCG 2 cut(s) 183, 425
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 5 cut(s) 89, 181, 272, 376, 549
HpyCH4V TGCA 4 cut(s) 53, 518, 622, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 619
HpyF3I CTNAG 2 cut(s) 341, 434
HpySE526I ACGT 5 cut(s) 89, 181, 272, 376, 549
Hsp92II CATG 5 cut(s) 20, 271, 352, 570, 618
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 2 cut(s) 327, 412
Kzo9I GATC 4 cut(s) 145, 187, 327, 412
LmnI GCTCC 1 cut(s) 306
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 2 cut(s) 138, 527
MaeI CTAG 2 cut(s) 291, 366
MaeII ACGT 5 cut(s) 89, 181, 272, 376, 549
MaeIII GTNAC 1 cut(s) 541
MalI GATC 4 cut(s) 147, 189, 329, 414
MboI GATC 4 cut(s) 145, 187, 327, 412
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 2 cut(s) 314, 440
MnlI CCTC 2 cut(s) 592, 653
MseI TTAA 3 cut(s) 165, 581, 694
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 619
NcoI CCATGG 1 cut(s) 348
NdeII GATC 4 cut(s) 145, 187, 327, 412
NlaIII CATG 5 cut(s) 20, 271, 352, 570, 618
NlaIV GGNNCC 1 cut(s) 80
NmuCI GTSAC 1 cut(s) 541
PagI TCATGA 1 cut(s) 267
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 2 cut(s) 124, 372
PpuMI RGGWCCY 1 cut(s) 322
PsiI TTATAA 2 cut(s) 246, 279
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 322
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 2 cut(s) 78, 322
PspPPI RGGWCCY 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 325
RsaI GTAC 3 cut(s) 69, 406, 638
RsaNI GTAC 3 cut(s) 68, 405, 637
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 3 cut(s) 165, 581, 694
SatI GCNGC 2 cut(s) 123, 371
Sau3AI GATC 4 cut(s) 145, 187, 327, 412
Sau96I GGNCC 2 cut(s) 78, 322
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 2 cut(s) 138, 527
SinI GGWCC 2 cut(s) 78, 322
Sse9I AATT 2 cut(s) 314, 440
SsiI CCGC 1 cut(s) 122
SspI AATATT 1 cut(s) 204
SspMI CTAG 2 cut(s) 291, 366
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 5 cut(s) 92, 184, 275, 379, 552
TaqI TCGA 7 cut(s) 5, 105, 213, 420, 445, 501, 657
TasI AATT 2 cut(s) 314, 440
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 165, 581, 694
Tru9I TTAA 3 cut(s) 165, 581, 694
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 6 cut(s) 33, 148, 300, 411, 555, 620
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 78, 322
XapI RAATTY 1 cut(s) 440
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XspI CTAG 2 cut(s) 291, 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.