Rmu_sc0003221.1_g000044

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003221.1
OG0025554
Physical Location & Seq
Reverse (-)
197130 .. 199603
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
atgtttcccggctttaagggaatgattccgattatttggatagtgaaatcaggcctgtctcttcaagatgagggggataggtttatctttcaatttgatagggagtcagagagaaacaagatccttcatggaggattgtggcactataagaacatgatgatggttctagtagagtatgacggaattgtacatccacaggctgttccgttgaagatattggagacctgggttggagtatgtgggctagcaccatggctgcgcaacaaggccagatccagcaaccagaaatggatattgaaaaagattccagaacctccgaaaagcctttcaattttatccaaaatatcatattctttcccaatcatgttaaaccatggattgaaatttaagttcgaagaaggaagcggcgtcgtcgtcggacgagccatattgacacaaattgggtcttctgtgaggcttagaatcctcgatcatttccatctcggtcttttttggggggattacggtggctggtactatgttgaggaaacggaagtttgtggcggctggcgtgtcttggatggttcctcgctggatcgtttggaatccaataggatcgaaattgtccgatctggtttaggtcttagagatccgagatggggaggggaagaatcgggaggggtggccctcatcagtggcgtgacagggatggtggtgcgatggtccggcggagtaggcttttgtcggcggggctgggatcctgtagagtccgatcggattgaaatgacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283

Protein Analysis

256

Amino Acids

28.92

Weight (kDa)

8.73

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025554)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003221.1_g000044
rosa_rugosa Rorug02G0109300 Rorug07G0086000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 260
AciI CCGC 4 cut(s) 405, 543, 708, 727
AclWI GGATC 7 cut(s) 115, 267, 582, 602, 623, 731, 744
AcsI RAATTY 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 2 cut(s) 189, 515
AfiI CCNNNNNNNGG 1 cut(s) 638
AgsI TTSAA 7 cut(s) 65, 92, 211, 298, 330, 382, 761
AjnI CCWGG 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 63, 215
AlwI GGATC 7 cut(s) 115, 267, 582, 602, 623, 731, 744
AoxI GGCC 3 cut(s) 52, 267, 663
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 1 cut(s) 383
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 664, 702
AsuC2I CCSGG 1 cut(s) 9
AsuII TTCGAA 1 cut(s) 393
AsuNHI GCTAGC 1 cut(s) 244
AvaII GGWCC 1 cut(s) 702
BamHI GGATCC 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 243
BccI CCATC 6 cut(s) 154, 486, 554, 630, 682, 693
BciT130I CCWGG 1 cut(s) 226
BcnI CCSGG 1 cut(s) 9
BcoDI GTCTC 2 cut(s) 63, 215
BfaI CTAG 2 cut(s) 167, 245
BfmI CTRYAG 1 cut(s) 741
BisI GCNGC 3 cut(s) 257, 406, 544
BlsI GCNGC 3 cut(s) 258, 407, 545
Bme1390I CCNGG 2 cut(s) 9, 226
Bme18I GGWCC 1 cut(s) 702
BmgT120I GGNCC 2 cut(s) 664, 702
BmiI GGNNCC 2 cut(s) 565, 738
BmrFI CCNGG 2 cut(s) 9, 226
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 225, 251, 373
Bsc4I CCNNNNNNNGG 1 cut(s) 638
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 3 cut(s) 225, 251, 373
BseGI GGATG 3 cut(s) 190, 565, 693
BseLI CCNNNNNNNGG 1 cut(s) 638
BseXI GCAGC 1 cut(s) 243
BseYI CCCAGC 1 cut(s) 732
Bsh1285I CGRYCG 1 cut(s) 754
BshFI GGCC 3 cut(s) 54, 269, 665
BsiEI CGRYCG 1 cut(s) 754
BsiSI CCGG 2 cut(s) 9, 705
BslI CCNNNNNNNGG 1 cut(s) 638
BsmAI GTCTC 2 cut(s) 63, 215
BsnI GGCC 3 cut(s) 54, 269, 665
Bso31I GGTCTC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 393
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
Bsp19I CCATGG 2 cut(s) 251, 373
BspACI CCGC 4 cut(s) 405, 543, 708, 727
BspANI GGCC 3 cut(s) 54, 269, 665
BspLI GGNNCC 2 cut(s) 565, 738
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 7 cut(s) 115, 267, 582, 602, 623, 731, 744
BspT104I TTCGAA 1 cut(s) 393
BspTNI GGTCTC 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 225, 251, 373
BssMI GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
BssNI GRCGYC 1 cut(s) 408
BssT1I CCWWGG 2 cut(s) 251, 373
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 506
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 408
BstAUI TGTACA 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 246, 548
BstDEI CTNAG 2 cut(s) 458, 623
BstDSI CCRYGG 2 cut(s) 251, 373
BstF5I GGATG 3 cut(s) 190, 565, 693
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 9 cut(s) 123, 275, 472, 577, 597, 611, 631, 739, 754
BstMAI GTCTC 2 cut(s) 63, 215
BstMBI GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
BstMCI CGRYCG 1 cut(s) 754
BstMWI GCNNNNNNNGC 1 cut(s) 714
BstNI CCWGG 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 7, 224
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 1 cut(s) 243
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 4 cut(s) 120, 272, 628, 736
BstYI RGATCY 4 cut(s) 120, 272, 628, 736
BsuRI GGCC 3 cut(s) 54, 269, 665
BtgI CCRYGG 2 cut(s) 251, 373
BtgZI GCGATG 1 cut(s) 712
BtsCI GGATG 3 cut(s) 190, 565, 693
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 246, 548
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 664, 702
CseI GACGC 1 cut(s) 397
Csp6I GTAC 2 cut(s) 188, 514
CviAII CATG 5 cut(s) 128, 154, 252, 364, 374
CviQI GTAC 2 cut(s) 188, 514
DdeI CTNAG 2 cut(s) 458, 623
DpnI GATC 9 cut(s) 122, 274, 471, 576, 596, 610, 630, 738, 753
DpnII GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EciI GGCGGA 1 cut(s) 723
Eco130I CCWWGG 2 cut(s) 251, 373
Eco147I AGGCCT 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 702
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 2 cut(s) 251, 373
ErhI CCWWGG 2 cut(s) 251, 373
FaeI CATG 5 cut(s) 131, 157, 255, 367, 377
FatI CATG 5 cut(s) 127, 153, 251, 363, 373
FauI CCCGC 1 cut(s) 720
Fnu4HI GCNGC 3 cut(s) 257, 406, 544
FokI GGATG 3 cut(s) 177, 572, 700
Fsp4HI GCNGC 3 cut(s) 257, 406, 544
FspBI CTAG 2 cut(s) 167, 245
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 1 cut(s) 260
GluI GCNGC 3 cut(s) 257, 406, 544
GsaI CCCAGC 1 cut(s) 736
HaeIII GGCC 3 cut(s) 54, 269, 665
HapII CCGG 2 cut(s) 9, 705
HgaI GACGC 1 cut(s) 397
HhaI GCGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 5 cut(s) 131, 157, 255, 367, 377
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HinfI GANTC 7 cut(s) 25, 104, 304, 462, 584, 650, 746
HpaII CCGG 2 cut(s) 9, 705
Hpy188I TCNGA 8 cut(s) 30, 109, 318, 419, 608, 633, 751, 756
Hpy188III TCNNGA 3 cut(s) 65, 308, 654
Hpy99I CGWCG 3 cut(s) 413, 416, 419
HpyAV CCTTC 2 cut(s) 134, 392
HpyCH4III ACNGT 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 714
HpyF3I CTNAG 2 cut(s) 458, 623
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 5 cut(s) 131, 157, 255, 367, 377
HspAI GCGC 1 cut(s) 259
Kzo9I GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
Lsp1109I GCAGC 1 cut(s) 243
MaeI CTAG 2 cut(s) 167, 245
MaeIII GTNAC 1 cut(s) 679
MalI GATC 9 cut(s) 122, 274, 471, 576, 596, 610, 630, 738, 753
MboI GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
MboII GAAGA 5 cut(s) 53, 223, 407, 438, 659
MflI RGATCY 4 cut(s) 120, 272, 628, 736
MluCI AATT 6 cut(s) 92, 183, 330, 383, 438, 600
MlyI GAGTC 2 cut(s) 113, 755
MmeI TCCRAC 2 cut(s) 211, 397
MseI TTAA 3 cut(s) 15, 368, 387
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 9, 705
MspR9I CCNGG 2 cut(s) 9, 226
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 714
NciI CCSGG 1 cut(s) 9
NcoI CCATGG 2 cut(s) 251, 373
NdeII GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 5 cut(s) 131, 157, 255, 367, 377
NlaIV GGNNCC 2 cut(s) 565, 738
NmuCI GTSAC 1 cut(s) 679
NsbI TGCGCA 1 cut(s) 260
NspV TTCGAA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 54
PfeI GAWTC 5 cut(s) 25, 304, 462, 584, 650
PkrI GCNGC 3 cut(s) 258, 407, 545
Ple19I CGATCG 1 cut(s) 754
PleI GAGTC 2 cut(s) 112, 754
PpsI GAGTC 2 cut(s) 112, 754
Psp6I CCWGG 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 2 cut(s) 565, 738
PspPI GGNCC 2 cut(s) 664, 702
PsuI RGATCY 4 cut(s) 120, 272, 628, 736
PvuI CGATCG 1 cut(s) 754
RsaI GTAC 2 cut(s) 189, 515
RsaNI GTAC 2 cut(s) 188, 514
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 3 cut(s) 15, 368, 387
SatI GCNGC 3 cut(s) 257, 406, 544
Sau3AI GATC 9 cut(s) 120, 272, 469, 574, 594, 608, 628, 736, 751
Sau96I GGNCC 2 cut(s) 664, 702
SchI GAGTC 2 cut(s) 113, 755
ScrFI CCNGG 2 cut(s) 9, 226
SetI ASST 4 cut(s) 83, 227, 316, 622
SfcI CTRYAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 393
SinI GGWCC 1 cut(s) 702
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 6 cut(s) 92, 183, 330, 383, 438, 600
SseBI AGGCCT 1 cut(s) 54
SsiI CCGC 4 cut(s) 405, 543, 708, 727
SspMI CTAG 2 cut(s) 167, 245
StuI AGGCCT 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 7, 224
StyI CCWWGG 2 cut(s) 251, 373
TaaI ACNGT 1 cut(s) 506
TaqI TCGA 3 cut(s) 393, 468, 597
TaqII GACCGA 1 cut(s) 473
TasI AATT 6 cut(s) 92, 183, 330, 383, 438, 600
TatI WGTACW 1 cut(s) 187
TauI GCSGC 2 cut(s) 408, 546
TfiI GAWTC 5 cut(s) 25, 304, 462, 584, 650
Tru1I TTAA 3 cut(s) 15, 368, 387
Tru9I TTAA 3 cut(s) 15, 368, 387
TscAI CASTG 1 cut(s) 679
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 1 cut(s) 116
TspGWI ACGGA 3 cut(s) 195, 195, 545
TspRI CASTG 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 702
XapI RAATTY 1 cut(s) 383
XspI CTAG 2 cut(s) 167, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.