Rmu_sc0003223.1_g000001

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003223.1
N/A
Physical Location & Seq
Reverse (-)
1 .. 940
940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
atgcaagcctccctctacttggacaccaaaactctcacatggccttcacgtcgccacacctcaacccaccacatatttcggccaacacccacagtcagattctggccattgcctctagtctccgccgtggccacgtcacctccacctctaactcggcagaagacccactcgttgccaccggaaaaggtggagctcttaaagtcactagagggttgggcctcccagacaatcctcccatttttgaagccagtcgatgagtgctggcaacccagtaccttcttgccagacccatcgctccccaaagaggagttcttggaccgtgtacgggcccttagggaccggacagatgggctacctgacgagtactttgtggtgctggtcggggatatgatcaccgaggaggtgctgcctgcgtaccagagtatgctcaatgggctcgacggggtgagagacgagtctggagttagctcgagcccgtgggcgcaatggattcgagcttggtctgctgaggaaaaccgccacggcgatctgctcaaaacctttctgtacctgtccggtcgggttgatatgttcaagatcgagaagactattcagaaccttattggagctggcatg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

205

Amino Acids

23.33

Weight (kDa)

5.96

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 205 1.1e-60 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 123, 517
AcoI YGGCCR 3 cut(s) 80, 104, 129
AfaI GTAC 5 cut(s) 274, 324, 365, 416, 548
AfiI CCNNNNNNNGG 3 cut(s) 19, 304, 325
AgsI TTSAA 2 cut(s) 244, 574
AjiI CACGTC 2 cut(s) 50, 135
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 4 cut(s) 193, 468, 497, 608
AluI AGCT 4 cut(s) 193, 468, 497, 608
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 124, 444
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 6 cut(s) 41, 80, 104, 129, 216, 327
ApaI GGGCCC 1 cut(s) 331
ApeKI GCWGC 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 5 cut(s) 216, 316, 327, 328, 337
AsuHPI GGTGA 3 cut(s) 129, 385, 457
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 2 cut(s) 316, 337
AxyI CCTNAGG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 331
BalI TGGCCA 2 cut(s) 106, 131
BanII GRGCYC 4 cut(s) 195, 331, 438, 476
BbsI GAAGAC 2 cut(s) 167, 590
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 393
BccI CCATC 2 cut(s) 298, 341
BceAI ACGGC 2 cut(s) 110, 538
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BclI TGATCA 1 cut(s) 390
BcoDI GTCTC 2 cut(s) 124, 444
BfaI CTAG 2 cut(s) 116, 206
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
BmcAI AGTACT 1 cut(s) 365
Bme18I GGWCC 2 cut(s) 316, 337
BmeT110I CYCGRG 1 cut(s) 469
BmgBI CACGTC 2 cut(s) 50, 135
BmgT120I GGNCC 5 cut(s) 216, 316, 327, 328, 337
BmiI GGNNCC 2 cut(s) 329, 338
BmrI ACTGGG 1 cut(s) 264
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 167, 590
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 507
BsaJI CCNNGG 4 cut(s) 126, 396, 476, 520
BsaWI WCCGGW 3 cut(s) 178, 339, 554
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 304, 325
Bse1I ACTGG 2 cut(s) 248, 270
Bse21I CCTNAGG 1 cut(s) 332
Bse3DI GCAATG 2 cut(s) 107, 491
BseDI CCNNGG 4 cut(s) 126, 396, 476, 520
BseLI CCNNNNNNNGG 3 cut(s) 19, 304, 325
BseMI GCAATG 2 cut(s) 107, 491
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 248, 270
BseRI GAGGAG 2 cut(s) 320, 413
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 6 cut(s) 43, 82, 106, 131, 218, 329
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 3 cut(s) 179, 340, 555
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 19, 304, 325
BsmAI GTCTC 2 cut(s) 124, 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 6 cut(s) 43, 82, 106, 131, 218, 329
BsoBI CYCGRG 1 cut(s) 469
Bsp120I GGGCCC 1 cut(s) 327
Bsp1286I GDGCHC 4 cut(s) 195, 331, 438, 476
Bsp143I GATC 3 cut(s) 390, 526, 576
BspACI CCGC 2 cut(s) 123, 517
BspANI GGCC 6 cut(s) 43, 82, 106, 131, 218, 329
BspCNI CTCAG 1 cut(s) 499
BspLI GGNNCC 2 cut(s) 329, 338
BsrDI GCAATG 2 cut(s) 107, 491
BsrI ACTGG 2 cut(s) 248, 270
BssECI CCNNGG 4 cut(s) 126, 396, 476, 520
BssMI GATC 3 cut(s) 390, 526, 576
Bst4CI ACNGT 2 cut(s) 94, 320
BstC8I GCNNGC 4 cut(s) 6, 263, 411, 610
BstDEI CTNAG 2 cut(s) 332, 507
BstDSI CCRYGG 3 cut(s) 126, 476, 520
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 3 cut(s) 393, 529, 579
BstMAI GTCTC 2 cut(s) 124, 444
BstMBI GATC 3 cut(s) 390, 526, 576
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 2 cut(s) 433, 503
BstSLI GKGCMC 1 cut(s) 331
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 2 cut(s) 167, 590
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 6 cut(s) 43, 82, 106, 131, 218, 329
BtgI CCRYGG 3 cut(s) 126, 476, 520
BtgZI GCGATG 1 cut(s) 276
BtrI CACGTC 2 cut(s) 50, 135
Cac8I GCNNGC 4 cut(s) 6, 263, 411, 610
CaiI CAGNNNCTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 5 cut(s) 216, 316, 327, 328, 337
Csp6I GTAC 5 cut(s) 273, 323, 364, 415, 547
CviAII CATG 2 cut(s) 39, 613
CviQI GTAC 5 cut(s) 273, 323, 364, 415, 547
DdeI CTNAG 2 cut(s) 332, 507
DpnI GATC 3 cut(s) 392, 528, 578
DpnII GATC 3 cut(s) 390, 526, 576
EaeI YGGCCR 3 cut(s) 80, 104, 129
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 4 cut(s) 195, 331, 438, 476
Eco47I GGWCC 2 cut(s) 316, 337
Eco53kI GAGCTC 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 328
EcoT38I GRGCYC 4 cut(s) 195, 331, 438, 476
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 1 cut(s) 42
FaiI YATR 6 cut(s) 40, 74, 389, 425, 569, 614
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 38
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 1 cut(s) 407
FriOI GRGCYC 4 cut(s) 195, 331, 438, 476
Fsp4HI GCNGC 1 cut(s) 407
FspBI CTAG 2 cut(s) 116, 206
GlaI GCGC 1 cut(s) 483
GluI GCNGC 1 cut(s) 407
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 6 cut(s) 43, 82, 106, 131, 218, 329
HapII CCGG 3 cut(s) 179, 340, 555
HhaI GCGC 1 cut(s) 484
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HinfI GANTC 3 cut(s) 99, 455, 490
HpaII CCGG 3 cut(s) 179, 340, 555
HphI GGTGA 3 cut(s) 129, 385, 457
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 2 cut(s) 98, 594
Hpy188III TCNNGA 3 cut(s) 459, 574, 580
Hpy8I GTNNAC 1 cut(s) 323
Hpy99I CGWCG 2 cut(s) 54, 443
HpyAV CCTTC 2 cut(s) 54, 286
HpyCH4III ACNGT 2 cut(s) 94, 320
HpyCH4IV ACGT 2 cut(s) 49, 134
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 433, 503
HpyF3I CTNAG 2 cut(s) 332, 507
HpySE526I ACGT 2 cut(s) 49, 134
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 3 cut(s) 390, 526, 576
LmnI GCTCC 3 cut(s) 190, 300, 605
Lsp1109I GCAGC 1 cut(s) 393
MaeI CTAG 2 cut(s) 116, 206
MaeII ACGT 2 cut(s) 49, 134
MaeIII GTNAC 2 cut(s) 135, 201
MalI GATC 3 cut(s) 392, 528, 578
MboI GATC 3 cut(s) 390, 526, 576
MboII GAAGA 2 cut(s) 172, 595
MhlI GDGCHC 4 cut(s) 195, 331, 438, 476
MlsI TGGCCA 2 cut(s) 106, 131
MluNI TGGCCA 2 cut(s) 106, 131
MlyI GAGTC 1 cut(s) 464
Mox20I TGGCCA 2 cut(s) 106, 131
MscI TGGCCA 2 cut(s) 106, 131
MseI TTAA 1 cut(s) 197
Msp20I TGGCCA 2 cut(s) 106, 131
MspI CCGG 3 cut(s) 179, 340, 555
MwoI GCNNNNNNNGC 2 cut(s) 433, 503
NdeII GATC 3 cut(s) 390, 526, 576
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 2 cut(s) 329, 338
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 2 cut(s) 135, 201
PaeR7I CTCGAG 1 cut(s) 469
PfeI GAWTC 2 cut(s) 99, 490
PkrI GCNGC 1 cut(s) 408
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp124BI GAGCTC 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 329, 338
PspOMI GGGCCC 1 cut(s) 327
PspPI GGNCC 5 cut(s) 216, 316, 327, 328, 337
PspXI VCTCGAGB 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 102
RsaI GTAC 5 cut(s) 274, 324, 365, 416, 548
RsaNI GTAC 5 cut(s) 273, 323, 364, 415, 547
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 1 cut(s) 407
Sau3AI GATC 3 cut(s) 390, 526, 576
Sau96I GGNCC 5 cut(s) 216, 316, 327, 328, 337
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 1 cut(s) 464
SduI GDGCHC 4 cut(s) 195, 331, 438, 476
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 316, 337
SlaI CTCGAG 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SsiI CCGC 2 cut(s) 123, 517
SspMI CTAG 2 cut(s) 116, 206
SstI GAGCTC 1 cut(s) 195
TaaI ACNGT 2 cut(s) 94, 320
TaiI ACGT 2 cut(s) 52, 137
TaqI TCGA 5 cut(s) 252, 438, 470, 493, 579
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 2 cut(s) 99, 490
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseFI GTSAC 2 cut(s) 135, 201
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 135, 201
VpaK11BI GGWCC 2 cut(s) 316, 337
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 2 cut(s) 116, 206
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.