Rmu_sc0003264.1_g000005

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003264.1
N/A
Physical Location & Seq
Forward (+)
14344 .. 15831
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1488 bp
atgaaggatcaaggaaccgcccctaatgttgccaccttcaacaccctaatacatgcactttgccagtcaggtaagagggaaaaggcccaaagattcttgtctttcatggctgagagtggaatttcaccaaatgtatatacatataatatcctaatcaacagtcactgcagggaagaaagaatacaagaagcagtttctgtgttagaaaagatgactagcaaaggcataaagcctactgttgtcacttttaactccttaatttctgcttcatgcaagtctggtcaatgggaaaacgctgtacagttatttataagtatgattgaatttggaaccttgcctaatattgttacattcaatgctgtgttggatgctttatgcaagagagggaagacatcagaggcacttaatcttttcgaagaaatgatccgtagaggtgtaaagcctaatcttttcacctacaactccttaattcatggattatgcctttctagcaagtggagagaggcaacaagcttgcttgatagaatgatggatgaaggagtctcaccagatattgtaaccttaacctctgtaatagatgctctttgcaaggagagaaggaccaaggacgccctcactgttttacagctaatgactcaaagaggtctaagtcctaacgttatcacttacagctgtctaatttatggattgtgccttgcttgccaatggagagaggcaacatgcttgcttaatagaatgatggatgaaggaatcccgccggatgttgtaaccttcaactctttaatagatgctctttgcaaggatagacggaccaaggacgcactcactgttttacagctaatgactcaaagaggtccaagtcctgacgtgatcacttacaactgtctagttcgtggattgtgccgttctggccaatggagagaagcaacaggcttgcttaatagaatgatggatgaaggagtcccaccaaatgttgtaaccttcaactctttaatagatgctctttgcaaggagagaaggaccaaggacgccctcactgttttacagctaatggctgaaagaggtctaagtcctgacgttatcacttacaactgtcttatttatggcatgtgcaatacatcacaatggggtgaagccacaaggttgtatgatgaaatggttggtctgggcatcttacctaatatgataactttgacaacacttttcgatactctctgcaaagaagggatgacagaagacgctcgcaaagcagttgaggcaacatttcaatatggtatggaggttatcaagatcacattcaacatgctgcttcatgattattgtttgcgtggaaaaagtgacaaggcagagaaggtcattagttttatggtgttgaagggtcatgttcctgatattgcttcctgtacaaccttggacaataactttgtaaaagttaacagtatagatgaagctttgagggttgtaaaggaaatgatccaaaagggttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

495

Amino Acids

55.37

Weight (kDa)

7.38

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 1 - 97 1.8e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 1 - 53 1.9e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 3 - 104 6.8e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 7 - 36 1.6e-08 PPR repeat
PPR_2 PF13041 8 - 57 8.6e-18 PPR repeat family
PPR_1 PF12854 40 - 72 1.3e-12 PPR repeat
PPR PF01535 46 - 76 1e-07 PPR repeat
TPR_24 PF23276 46 - 140 1.4e-08 Fungal tetratrico peptide repeats
PPR_2 PF13041 47 - 92 8.6e-15 PPR repeat family
PPR_3 PF13812 66 - 127 1.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 74 - 106 2.4e-10 PPR repeat
PPR_2 PF13041 78 - 127 6.7e-15 PPR repeat family
PPR PF01535 81 - 110 1.6e-07 PPR repeat
PPR_3 PF13812 110 - 158 1.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 112 - 141 9.8e-10 PPR repeat
PPR_2 PF13041 113 - 161 8.9e-21 PPR repeat family
PPR PF01535 116 - 146 2.3e-09 PPR repeat
PPR_long PF17177 122 - 258 1.4e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 137 - 187 1.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 144 - 176 7.4e-11 PPR repeat
PPR_2 PF13041 151 - 197 3.6e-13 PPR repeat family
PPR PF01535 151 - 181 4.5e-07 PPR repeat
PPR_3 PF13812 171 - 227 8.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 180 - 211 3.3e-07 PPR repeat
PPR_2 PF13041 183 - 231 1.4e-14 PPR repeat family
TPR_24 PF23276 204 - 312 9e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 214 - 246 1.3e-07 PPR repeat
PPR_2 PF13041 225 - 267 1e-10 PPR repeat family
PPR_3 PF13812 242 - 297 3.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 250 - 281 2.2e-10 PPR repeat
PPR_2 PF13041 253 - 302 2e-16 PPR repeat family
PPR PF01535 256 - 285 5.4e-06 PPR repeat
PPR_1 PF12854 284 - 316 3.9e-11 PPR repeat
PPR_2 PF13041 288 - 337 1.8e-18 PPR repeat family
PPR PF01535 291 - 321 8.8e-08 PPR repeat
PPR_3 PF13812 312 - 367 6.9e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 320 - 351 3e-10 PPR repeat
PPR_2 PF13041 323 - 372 5.6e-17 PPR repeat family
PPR PF01535 326 - 356 1.5e-06 PPR repeat
PPR_1 PF12854 354 - 387 1.8e-09 PPR repeat
PPR_2 PF13041 358 - 407 3.5e-15 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AciI CCGC 2 cut(s) 18, 755
AclI AACGTT 1 cut(s) 657
AclWI GGATC 3 cut(s) 15, 418, 1468
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 2 cut(s) 120, 323
AcyI GRCGYC 2 cut(s) 609, 1029
AfaI GTAC 2 cut(s) 300, 1405
AgsI TTSAA 8 cut(s) 40, 323, 355, 775, 985, 1268, 1300, 1375
AjiI CACGTC 1 cut(s) 868
AluBI AGCT 6 cut(s) 513, 628, 672, 838, 1048, 1451
AluI AGCT 6 cut(s) 513, 628, 672, 838, 1048, 1451
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 3 cut(s) 15, 418, 1468
AlwNI CAGNNNCTG 2 cut(s) 165, 197
AoxI GGCC 2 cut(s) 84, 910
ApeKI GCWGC 1 cut(s) 1306
ApoI RAATTY 2 cut(s) 120, 323
ArsI GACNNNNNNTTYG 2 cut(s) 1406, 1438
AspS9I GGNCC 5 cut(s) 85, 600, 810, 854, 1020
AsuHPI GGTGA 4 cut(s) 117, 445, 537, 1142
AsuII TTCGAA 1 cut(s) 414
AvaII GGWCC 4 cut(s) 600, 810, 854, 1020
BalI TGGCCA 1 cut(s) 912
BarI GAAGNNNNNNTAC 2 cut(s) 165, 197
BbsI GAAGAC 2 cut(s) 395, 1242
BbvI GCAGC 1 cut(s) 1293
BccI CCATC 3 cut(s) 523, 733, 943
BceAI ACGGC 1 cut(s) 888
BclI TGATCA 1 cut(s) 870
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 3 cut(s) 216, 489, 887
BfmI CTRYAG 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 909
BisI GCNGC 1 cut(s) 1307
BlsI GCNGC 1 cut(s) 1308
Bme18I GGWCC 4 cut(s) 600, 810, 854, 1020
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 5 cut(s) 85, 600, 810, 854, 1020
BmiI GGNNCC 2 cut(s) 16, 331
BmsI GCATC 5 cut(s) 358, 568, 778, 988, 1179
BpiI GAAGAC 2 cut(s) 395, 1242
Bpu14I TTCGAA 1 cut(s) 414
BsaHI GRCGYC 2 cut(s) 609, 1029
BsaJI CCNNGG 4 cut(s) 603, 813, 1023, 1410
Bse1I ACTGG 1 cut(s) 64
BseDI CCNNGG 4 cut(s) 603, 813, 1023, 1410
BseGI GGATG 6 cut(s) 373, 538, 748, 766, 958, 1233
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 64
BseXI GCAGC 1 cut(s) 1293
BshFI GGCC 2 cut(s) 86, 912
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 947
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 1 cut(s) 947
BsnI GGCC 2 cut(s) 86, 912
Bsp119I TTCGAA 1 cut(s) 414
Bsp1407I TGTACA 2 cut(s) 298, 1403
Bsp143I GATC 5 cut(s) 7, 423, 870, 1290, 1473
BspACI CCGC 2 cut(s) 18, 755
BspANI GGCC 2 cut(s) 86, 912
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 2 cut(s) 16, 331
BspMAI CTGCAG 1 cut(s) 170
BspPI GGATC 3 cut(s) 15, 418, 1468
BspT104I TTCGAA 1 cut(s) 414
BsrGI TGTACA 2 cut(s) 298, 1403
BsrI ACTGG 1 cut(s) 64
BssECI CCNNGG 4 cut(s) 603, 813, 1023, 1410
BssMI GATC 5 cut(s) 7, 423, 870, 1290, 1473
BssNI GRCGYC 2 cut(s) 609, 1029
BssT1I CCWWGG 4 cut(s) 603, 813, 1023, 1410
Bst4CI ACNGT 9 cut(s) 161, 238, 303, 619, 829, 884, 1039, 1094, 1439
BstACI GRCGYC 2 cut(s) 609, 1029
BstAUI TGTACA 2 cut(s) 298, 1403
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 5 cut(s) 515, 700, 725, 935, 1243
BstDEI CTNAG 3 cut(s) 111, 647, 1067
BstF5I GGATG 6 cut(s) 373, 538, 748, 766, 958, 1233
BstKTI GATC 5 cut(s) 10, 426, 873, 1293, 1476
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 5 cut(s) 7, 423, 870, 1290, 1473
BstMWI GCNNNNNNNGC 5 cut(s) 489, 699, 909, 1247, 1256
BstNSI RCATGY 4 cut(s) 56, 723, 1111, 1306
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 1293
BstV2I GAAGAC 2 cut(s) 395, 1242
BsuRI GGCC 2 cut(s) 86, 912
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 6 cut(s) 373, 538, 748, 766, 958, 1233
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 4 cut(s) 163, 615, 825, 1035
Cac8I GCNNGC 5 cut(s) 515, 700, 725, 935, 1243
CaiI CAGNNNCTG 2 cut(s) 165, 197
CciI TCATGA 1 cut(s) 1312
Cfr13I GGNCC 5 cut(s) 85, 600, 810, 854, 1020
CseI GACGC 4 cut(s) 617, 827, 1037, 1247
Csp6I GTAC 2 cut(s) 299, 1404
CviAII CATG 9 cut(s) 53, 106, 270, 473, 720, 1108, 1303, 1313, 1382
CviQI GTAC 2 cut(s) 299, 1404
DdeI CTNAG 3 cut(s) 111, 647, 1067
DpnI GATC 5 cut(s) 9, 425, 872, 1292, 1475
DpnII GATC 5 cut(s) 7, 423, 870, 1290, 1473
EaeI YGGCCR 1 cut(s) 910
Eco130I CCWWGG 4 cut(s) 603, 813, 1023, 1410
Eco47I GGWCC 4 cut(s) 600, 810, 854, 1020
EcoT14I CCWWGG 4 cut(s) 603, 813, 1023, 1410
ErhI CCWWGG 4 cut(s) 603, 813, 1023, 1410
FaeI CATG 9 cut(s) 56, 109, 273, 476, 723, 1111, 1306, 1316, 1385
FaqI GGGAC 1 cut(s) 947
FatI CATG 9 cut(s) 52, 105, 269, 472, 719, 1107, 1302, 1312, 1381
FauI CCCGC 1 cut(s) 762
FbaI TGATCA 1 cut(s) 870
Fnu4HI GCNGC 1 cut(s) 1307
FokI GGATG 6 cut(s) 380, 545, 755, 773, 965, 1240
Fsp4HI GCNGC 1 cut(s) 1307
FspBI CTAG 3 cut(s) 216, 489, 887
GluI GCNGC 1 cut(s) 1307
HaeIII GGCC 2 cut(s) 86, 912
HapII CCGG 1 cut(s) 758
HgaI GACGC 4 cut(s) 617, 827, 1037, 1247
Hin1I GRCGYC 2 cut(s) 609, 1029
Hin1II CATG 9 cut(s) 56, 109, 273, 476, 723, 1111, 1306, 1316, 1385
HincII GTYRAC 1 cut(s) 1435
HindII GTYRAC 1 cut(s) 1435
HindIII AAGCTT 2 cut(s) 511, 1449
HinfI GANTC 6 cut(s) 93, 540, 634, 750, 844, 960
HpaI GTTAAC 1 cut(s) 1435
HpaII CCGG 1 cut(s) 758
HphI GGTGA 4 cut(s) 117, 445, 537, 1142
Hpy166II GTNNAC 1 cut(s) 1435
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 5 cut(s) 863, 1073, 1288, 1313, 1388
Hpy8I GTNNAC 1 cut(s) 1435
HpyCH4III ACNGT 9 cut(s) 161, 238, 303, 619, 829, 884, 1039, 1094, 1439
HpyCH4IV ACGT 3 cut(s) 657, 867, 1077
HpyCH4V TGCA 9 cut(s) 56, 168, 273, 378, 588, 798, 1008, 1113, 1218
HpyF10VI GCNNNNNNNGC 5 cut(s) 489, 699, 909, 1247, 1256
HpyF3I CTNAG 3 cut(s) 111, 647, 1067
HpySE526I ACGT 3 cut(s) 657, 867, 1077
Hsp92I GRCGYC 2 cut(s) 609, 1029
Hsp92II CATG 9 cut(s) 56, 109, 273, 476, 723, 1111, 1306, 1316, 1385
Ksp22I TGATCA 1 cut(s) 870
KspAI GTTAAC 1 cut(s) 1435
Kzo9I GATC 5 cut(s) 7, 423, 870, 1290, 1473
Lsp1109I GCAGC 1 cut(s) 1293
LweI GCATC 5 cut(s) 358, 568, 778, 988, 1179
MaeI CTAG 3 cut(s) 216, 489, 887
MaeII ACGT 3 cut(s) 657, 867, 1077
MaeIII GTNAC 7 cut(s) 161, 241, 346, 556, 766, 976, 1337
MalI GATC 5 cut(s) 9, 425, 872, 1292, 1475
MboI GATC 5 cut(s) 7, 423, 870, 1290, 1473
MboII GAAGA 4 cut(s) 185, 400, 428, 1247
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 5 cut(s) 120, 258, 323, 468, 678
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 4 cut(s) 549, 628, 838, 969
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 912
MscI TGGCCA 1 cut(s) 912
MslI CAYNNNNRTG 1 cut(s) 1123
Msp20I TGGCCA 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 672
MspI CCGG 1 cut(s) 758
MwoI GCNNNNNNNGC 5 cut(s) 489, 699, 909, 1247, 1256
NdeII GATC 5 cut(s) 7, 423, 870, 1290, 1473
NlaIII CATG 9 cut(s) 56, 109, 273, 476, 723, 1111, 1306, 1316, 1385
NlaIV GGNNCC 2 cut(s) 16, 331
NmuCI GTSAC 3 cut(s) 161, 241, 1337
NspI RCATGY 4 cut(s) 56, 723, 1111, 1306
NspV TTCGAA 1 cut(s) 414
PagI TCATGA 1 cut(s) 1312
PfeI GAWTC 2 cut(s) 93, 750
PkrI GCNGC 1 cut(s) 1308
PleI GAGTC 4 cut(s) 548, 628, 838, 968
PpsI GAGTC 4 cut(s) 548, 628, 838, 968
PsiI TTATAA 1 cut(s) 311
Psp1406I AACGTT 1 cut(s) 657
PspN4I GGNNCC 2 cut(s) 16, 331
PspPI GGNCC 5 cut(s) 85, 600, 810, 854, 1020
PstI CTGCAG 1 cut(s) 170
PstNI CAGNNNCTG 2 cut(s) 165, 197
PvuII CAGCTG 1 cut(s) 672
RsaI GTAC 2 cut(s) 300, 1405
RsaNI GTAC 2 cut(s) 299, 1404
RseI CAYNNNNRTG 1 cut(s) 1123
SatI GCNGC 1 cut(s) 1307
Sau3AI GATC 5 cut(s) 7, 423, 870, 1290, 1473
Sau96I GGNCC 5 cut(s) 85, 600, 810, 854, 1020
SchI GAGTC 4 cut(s) 549, 628, 838, 969
SfaNI GCATC 5 cut(s) 358, 568, 778, 988, 1179
SfcI CTRYAG 1 cut(s) 166
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 4 cut(s) 600, 810, 854, 1020
SmiMI CAYNNNNRTG 1 cut(s) 1123
Sse9I AATT 5 cut(s) 120, 258, 323, 468, 678
SsiI CCGC 2 cut(s) 18, 755
SspI AATATT 1 cut(s) 343
SspMI CTAG 3 cut(s) 216, 489, 887
StyI CCWWGG 4 cut(s) 603, 813, 1023, 1410
TaaI ACNGT 9 cut(s) 161, 238, 303, 619, 829, 884, 1039, 1094, 1439
TaiI ACGT 3 cut(s) 660, 870, 1080
TaqI TCGA 2 cut(s) 414, 1206
TasI AATT 5 cut(s) 120, 258, 323, 468, 678
TatI WGTACW 2 cut(s) 298, 1403
TfiI GAWTC 2 cut(s) 93, 750
TscAI CASTG 4 cut(s) 170, 622, 832, 1042
TseFI GTSAC 3 cut(s) 161, 241, 1337
TseI GCWGC 1 cut(s) 1306
Tsp45I GTSAC 3 cut(s) 161, 241, 1337
TspGWI ACGGA 2 cut(s) 416, 823
TspRI CASTG 4 cut(s) 170, 622, 832, 1042
VpaK11BI GGWCC 4 cut(s) 600, 810, 854, 1020
XapI RAATTY 2 cut(s) 120, 323
XceI RCATGY 4 cut(s) 56, 723, 1111, 1306
XspI CTAG 3 cut(s) 216, 489, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.