Rmu_sc0003385.1_g000029

Belongs to the ATG8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003385.1
N/A
Physical Location & Seq
Reverse (-)
129868 .. 132644
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
atgacgaaaagcacctttaagcaagcacacgagtttgagaagaggcgtgctgaggcctcgaggattagagagaagtacccagatagaattccagtgattgtggagaaggcagagagaagtgatattcccaacattgacaagaaaaaatacctagtaccagctgatttgactgttggtcaatttgtctatgtcatccgcaagagaatcaaactgagtgcagaaaaggccatcttcatatttgtggacaatgtcctcccacctacaggagcagtaatgtctactatctatgatgaaaagaaggatggtgatggatttctatatgtttcatacagtgaactatcctgtccccgaactttggatgcaactcccaagacttgggactcggactttcaagttcgaggctggatcattagtgcaagtgtacatgtgacgttagctccggtgctccgtatgtgcttctctggaataccaaggatggagttcttctatatcctcgagtgtcgttggttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

170

Amino Acids

19.72

Weight (kDa)

9.1

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 14 - 112 2.7e-46 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 375
AccI GTMKAC 1 cut(s) 278
AciI CCGC 1 cut(s) 196
AclWI GGATC 1 cut(s) 413
AcsI RAATTY 1 cut(s) 87
AfaI GTAC 3 cut(s) 77, 156, 423
AfiI CCNNNNNNNGG 3 cut(s) 263, 355, 375
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 1 cut(s) 392
AhdI GACNNNNNGTC 1 cut(s) 174
AluBI AGCT 2 cut(s) 161, 437
AluI AGCT 2 cut(s) 161, 437
Alw21I GWGCWC 1 cut(s) 447
AlwI GGATC 1 cut(s) 413
Ama87I CYCGRG 2 cut(s) 58, 494
AoxI GGCC 2 cut(s) 54, 225
ApoI RAATTY 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 2 cut(s) 58, 494
BauI CACGAG 1 cut(s) 29
Bbv12I GWGCWC 1 cut(s) 447
BbvCI CCTCAGC 1 cut(s) 51
BccI CCATC 4 cut(s) 236, 296, 302, 469
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 261
BmeRI GACNNNNNGTC 1 cut(s) 174
BmeT110I CYCGRG 2 cut(s) 58, 494
BmsI GCATC 1 cut(s) 349
Bpu10I CCTNAGC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 470
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 355, 375
Bse1I ACTGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 470
BseGI GGATG 4 cut(s) 192, 307, 364, 480
BseLI CCNNNNNNNGG 3 cut(s) 263, 355, 375
BseMII CTCAG 2 cut(s) 42, 203
BseNI ACTGG 1 cut(s) 92
BsgI GTGCAG 1 cut(s) 237
BshFI GGCC 2 cut(s) 56, 227
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 2 cut(s) 58, 494
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 2 cut(s) 330, 392
BslI CCNNNNNNNGG 3 cut(s) 263, 355, 375
BsmFI GGGAC 2 cut(s) 330, 392
BsnI GGCC 2 cut(s) 56, 227
BsoBI CYCGRG 2 cut(s) 58, 494
Bsp1286I GDGCHC 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 421
Bsp143I GATC 1 cut(s) 405
BspACI CCGC 1 cut(s) 196
BspANI GGCC 2 cut(s) 56, 227
BspCNI CTCAG 2 cut(s) 43, 204
BspPI GGATC 1 cut(s) 413
BsrGI TGTACA 1 cut(s) 421
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 470
BssMI GATC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 470
Bst2BI CACGAG 1 cut(s) 29
Bst4CI ACNGT 2 cut(s) 172, 332
Bst6I CTCTTC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 24, 48
BstDEI CTNAG 2 cut(s) 51, 212
BstF5I GGATG 4 cut(s) 192, 307, 364, 480
BstKTI GATC 1 cut(s) 408
BstMBI GATC 1 cut(s) 405
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 261
BsuRI GGCC 2 cut(s) 56, 227
BtsCI GGATG 4 cut(s) 192, 307, 364, 480
BtsIMutI CAGTG 2 cut(s) 99, 337
Cac8I GCNNGC 2 cut(s) 24, 48
Csp6I GTAC 3 cut(s) 76, 155, 422
CviAII CATG 1 cut(s) 425
CviJI RGCY 5 cut(s) 56, 161, 227, 402, 437
CviKI_1 RGCY 5 cut(s) 56, 161, 227, 402, 437
CviQI GTAC 3 cut(s) 76, 155, 422
DdeI CTNAG 2 cut(s) 51, 212
DpnI GATC 1 cut(s) 407
DpnII GATC 1 cut(s) 405
DriI GACNNNNNGTC 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 470
Eco147I AGGCCT 1 cut(s) 56
Eco88I CYCGRG 2 cut(s) 58, 494
EcoRI GAATTC 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 470
FaeI CATG 1 cut(s) 428
FaiI YATR 9 cut(s) 189, 236, 288, 319, 321, 328, 426, 452, 489
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 330, 392
FatI CATG 1 cut(s) 424
FblI GTMKAC 1 cut(s) 278
FokI GGATG 4 cut(s) 179, 314, 371, 487
FspBI CTAG 1 cut(s) 152
HaeIII GGCC 2 cut(s) 56, 227
HapII CCGG 1 cut(s) 440
Hin1II CATG 1 cut(s) 428
HinfI GANTC 2 cut(s) 204, 380
HpaII CCGG 1 cut(s) 440
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 4 cut(s) 244, 279, 335, 422
Hpy188I TCNGA 1 cut(s) 385
Hpy188III TCNNGA 1 cut(s) 462
Hpy8I GTNNAC 4 cut(s) 244, 279, 335, 422
HpyAV CCTTC 2 cut(s) 100, 292
HpyCH4III ACNGT 2 cut(s) 172, 332
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 3 cut(s) 218, 362, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 2 cut(s) 51, 212
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 1 cut(s) 428
Kzo9I GATC 1 cut(s) 405
LmnI GCTCC 3 cut(s) 266, 442, 450
LpnPI CCDG 8 cut(s) 93, 105, 171, 249, 355, 388, 447, 453
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 152
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 427
MalI GATC 1 cut(s) 407
MboI GATC 1 cut(s) 405
MboII GAAGA 3 cut(s) 52, 223, 475
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 2 cut(s) 87, 179
MlyI GAGTC 1 cut(s) 374
MnlI CCTC 7 cut(s) 36, 46, 54, 67, 263, 392, 503
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 1 cut(s) 405
NlaIII CATG 1 cut(s) 428
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 428
PaeR7I CTCGAG 2 cut(s) 58, 494
PceI AGGCCT 1 cut(s) 56
PciI ACATGT 1 cut(s) 424
PfeI GAWTC 1 cut(s) 204
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 375
PleI GAGTC 1 cut(s) 374
PpsI GAGTC 1 cut(s) 374
PscI ACATGT 1 cut(s) 424
PspXI VCTCGAGB 2 cut(s) 58, 494
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 3 cut(s) 77, 156, 423
RsaNI GTAC 3 cut(s) 76, 155, 422
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 1 cut(s) 405
SchI GAGTC 1 cut(s) 374
SduI GDGCHC 1 cut(s) 447
SetI ASST 6 cut(s) 17, 153, 163, 262, 434, 439
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 261
Sfr274I CTCGAG 2 cut(s) 58, 494
SlaI CTCGAG 2 cut(s) 58, 494
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 58, 494
SmoI CTYRAG 2 cut(s) 58, 494
Sse9I AATT 2 cut(s) 87, 179
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 1 cut(s) 196
SspMI CTAG 1 cut(s) 152
StuI AGGCCT 1 cut(s) 56
StyI CCWWGG 1 cut(s) 470
TaaI ACNGT 2 cut(s) 172, 332
TaiI ACGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 59, 397, 495
TasI AATT 2 cut(s) 87, 179
TatI WGTACW 1 cut(s) 421
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 99, 337
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 223, 306, 315
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 99, 337
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 375
XapI RAATTY 1 cut(s) 87
XceI RCATGY 1 cut(s) 428
XhoI CTCGAG 2 cut(s) 58, 494
XmiI GTMKAC 1 cut(s) 278
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.