Rmu_sc0003413.1_g000060

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003413.1
N/A
Physical Location & Seq
Reverse (-)
277496 .. 280611
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003413.1_g000060.1.cds

Sequence Viewer

Length: 654 bp
atgcgtgcgcaggagcgcgtaggcgctgcaggctcaggcacgcagatgaggctagcgtggggggctgtaggctgcacacgggtgtgtaagggctgcaggggcagcgtgcagcgcagggtggctagtctagcatgggctaggtgcaacgataatttgcacacggctttgtgtcagaagggaattgagacctttagagatgataagcttagcagaggagacgacataccacaagagctcctcaaagcaattgtggagtccaaagtttcaatcatcgtcttctctcaaaactatgcttcgtcgaggtggtgcttagatgaactagtcaagatacttgaatgcagaaaattaaaaggacaagaggttagagcagttttctacaaggtggagccctcagatgtacgacaccaaagaggccccttcggtgacgcatttgctacgcttgatcaaagcaaatacaaggataacataggcaaatggaaggcagctcgtaaggaagcagcagatttgtctggatggcctttcaaggatggcgaatatatcattgtcgtcgtccacctccagcagctctcactacctcctctaaggcaccgtcattctccagatcctctacagtcacattcgcttccccaactcggaccacgtcgttttcagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

217

Amino Acids

24.45

Weight (kDa)

9.59

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 585
AccII CGCG 1 cut(s) 18
AclWI GGATC 1 cut(s) 596
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 1 cut(s) 632
AgsI TTSAA 3 cut(s) 267, 335, 523
AhlI ACTAGT 1 cut(s) 319
AjiI CACGTC 1 cut(s) 641
AleI CACNNNNGTG 2 cut(s) 80, 82
AluBI AGCT 4 cut(s) 205, 235, 485, 565
AluI AGCT 4 cut(s) 205, 235, 485, 565
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 179, 210
AlwI GGATC 1 cut(s) 596
AoxI GGCC 2 cut(s) 412, 515
ApeKI GCWGC 8 cut(s) 26, 72, 93, 102, 109, 482, 497, 562
AspLEI GCGC 4 cut(s) 10, 18, 26, 114
AspS9I GGNCC 2 cut(s) 413, 635
AsuHPI GGTGA 1 cut(s) 434
AsuNHI GCTAGC 1 cut(s) 52
AvaII GGWCC 1 cut(s) 635
BanI GGYRCC 1 cut(s) 585
BanII GRGCYC 2 cut(s) 237, 390
BbsI GAAGAC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 8 cut(s) 13, 59, 80, 114, 121, 494, 509, 574
BccI CCATC 2 cut(s) 507, 521
BceAI ACGGC 1 cut(s) 177
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 179, 210
BcuI ACTAGT 1 cut(s) 319
BfaI CTAG 5 cut(s) 53, 123, 128, 138, 320
BfmI CTRYAG 4 cut(s) 27, 66, 94, 608
BfoI RGCGCY 1 cut(s) 27
BisI GCNGC 8 cut(s) 27, 73, 94, 103, 110, 483, 498, 563
BlpI GCTNAGC 1 cut(s) 206
BlsI GCNGC 8 cut(s) 28, 74, 95, 104, 111, 484, 499, 564
Bme18I GGWCC 1 cut(s) 635
BmgBI CACGTC 1 cut(s) 641
BmgT120I GGNCC 2 cut(s) 413, 635
BmiI GGNNCC 3 cut(s) 387, 415, 587
BmtI GCTAGC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 268
BpmI CTGGAG 2 cut(s) 542, 582
Bpu10I CCTNAGC 1 cut(s) 34
Bpu1102I GCTNAGC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 632
BseGI GGATG 2 cut(s) 518, 532
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMII CTCAG 2 cut(s) 48, 405
BseRI GAGGAG 3 cut(s) 227, 228, 567
BseXI GCAGC 8 cut(s) 13, 59, 80, 114, 121, 494, 509, 574
BsgI GTGCAG 2 cut(s) 58, 128
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 2 cut(s) 414, 517
BshNI GGYRCC 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 632
BsmAI GTCTC 2 cut(s) 179, 210
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 2 cut(s) 414, 517
Bso31I GGTCTC 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 237, 390
Bsp143I GATC 2 cut(s) 442, 601
Bsp1720I GCTNAGC 1 cut(s) 206
BspANI GGCC 2 cut(s) 414, 517
BspCNI CTCAG 2 cut(s) 47, 404
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 387, 415, 587
BspMAI CTGCAG 2 cut(s) 31, 98
BspOI GCTAGC 1 cut(s) 56
BspPI GGATC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 179
BssMI GATC 2 cut(s) 442, 601
Bst4CI ACNGT 2 cut(s) 590, 612
BstC8I GCNNGC 5 cut(s) 6, 31, 41, 54, 107
BstDEI CTNAG 5 cut(s) 34, 206, 310, 391, 581
BstF5I GGATG 2 cut(s) 518, 532
BstFNI CGCG 1 cut(s) 18
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 4 cut(s) 10, 18, 26, 114
BstKTI GATC 2 cut(s) 445, 604
BstMAI GTCTC 2 cut(s) 179, 210
BstMBI GATC 2 cut(s) 442, 601
BstMWI GCNNNNNNNGC 7 cut(s) 30, 49, 62, 99, 102, 111, 128
BstSFI CTRYAG 4 cut(s) 27, 66, 94, 608
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 8 cut(s) 13, 59, 80, 114, 121, 494, 509, 574
BstV2I GAAGAC 1 cut(s) 268
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 2 cut(s) 414, 517
BtrI CACGTC 1 cut(s) 641
BtsCI GGATG 2 cut(s) 518, 532
Cac8I GCNNGC 5 cut(s) 6, 31, 41, 54, 107
CfoI GCGC 4 cut(s) 10, 18, 26, 114
Cfr13I GGNCC 2 cut(s) 413, 635
CseI GACGC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 398
CviAII CATG 1 cut(s) 132
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 5 cut(s) 34, 206, 310, 391, 581
DpnI GATC 2 cut(s) 444, 603
DpnII GATC 2 cut(s) 442, 601
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 2 cut(s) 237, 390
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 413
EcoT38I GRGCYC 2 cut(s) 237, 390
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 1 cut(s) 135
FaiI YATR 5 cut(s) 133, 224, 291, 467, 537
FatI CATG 1 cut(s) 131
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 8 cut(s) 27, 73, 94, 103, 110, 483, 498, 563
FokI GGATG 2 cut(s) 525, 539
FriOI GRGCYC 2 cut(s) 237, 390
Fsp4HI GCNGC 8 cut(s) 27, 73, 94, 103, 110, 483, 498, 563
FspBI CTAG 5 cut(s) 53, 123, 128, 138, 320
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 4 cut(s) 9, 17, 25, 113
GluI GCNGC 8 cut(s) 27, 73, 94, 103, 110, 483, 498, 563
GsuI CTGGAG 2 cut(s) 542, 582
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 2 cut(s) 414, 517
HgaI GACGC 1 cut(s) 434
HhaI GCGC 4 cut(s) 10, 18, 26, 114
Hin1II CATG 1 cut(s) 135
Hin6I GCGC 4 cut(s) 8, 16, 24, 112
HinP1I GCGC 4 cut(s) 8, 16, 24, 112
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 1 cut(s) 254
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 3 cut(s) 174, 394, 635
Hpy188III TCNNGA 3 cut(s) 325, 510, 599
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 3 cut(s) 301, 551, 645
HpyAV CCTTC 3 cut(s) 169, 427, 472
HpyCH4III ACNGT 2 cut(s) 590, 612
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 7 cut(s) 29, 75, 96, 109, 144, 157, 339
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 49, 62, 99, 102, 111, 128
HpyF3I CTNAG 5 cut(s) 34, 206, 310, 391, 581
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 1 cut(s) 135
HspAI GCGC 4 cut(s) 8, 16, 24, 112
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 2 cut(s) 442, 601
LmnI GCTCC 3 cut(s) 13, 240, 385
LpnPI CCDG 7 cut(s) 15, 21, 82, 100, 495, 572, 612
Lsp1109I GCAGC 8 cut(s) 13, 59, 80, 114, 121, 494, 509, 574
MaeI CTAG 5 cut(s) 53, 123, 128, 138, 320
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 2 cut(s) 422, 612
MalI GATC 2 cut(s) 444, 603
MboI GATC 2 cut(s) 442, 601
MboII GAAGA 1 cut(s) 268
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 2 cut(s) 237, 390
MluCI AATT 4 cut(s) 151, 180, 246, 344
MlyI GAGTC 1 cut(s) 263
MseI TTAA 1 cut(s) 347
MslI CAYNNNNRTG 3 cut(s) 44, 80, 82
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 341
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 7 cut(s) 30, 49, 62, 99, 102, 111, 128
NdeII GATC 2 cut(s) 442, 601
NheI GCTAGC 1 cut(s) 52
NlaIII CATG 1 cut(s) 135
NlaIV GGNNCC 3 cut(s) 387, 415, 587
NmuCI GTSAC 2 cut(s) 422, 612
NsbI TGCGCA 1 cut(s) 9
OliI CACNNNNGTG 2 cut(s) 80, 82
PctI GAATGC 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 639
PkrI GCNGC 8 cut(s) 28, 74, 95, 104, 111, 484, 499, 564
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 387, 415, 587
PspPI GGNCC 2 cut(s) 413, 635
PstI CTGCAG 2 cut(s) 31, 98
PsuI RGATCY 1 cut(s) 601
PsyI GACNNNGTC 1 cut(s) 639
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 3 cut(s) 44, 80, 82
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 8 cut(s) 27, 73, 94, 103, 110, 483, 498, 563
Sau3AI GATC 2 cut(s) 442, 601
Sau96I GGNCC 2 cut(s) 413, 635
SchI GAGTC 1 cut(s) 263
SduI GDGCHC 2 cut(s) 237, 390
SfcI CTRYAG 4 cut(s) 27, 66, 94, 608
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 3 cut(s) 44, 80, 82
SpeI ACTAGT 1 cut(s) 319
Sse9I AATT 4 cut(s) 151, 180, 246, 344
SspMI CTAG 5 cut(s) 53, 123, 128, 138, 320
SstI GAGCTC 1 cut(s) 237
TaaI ACNGT 2 cut(s) 590, 612
TaiI ACGT 1 cut(s) 643
TaqI TCGA 1 cut(s) 299
TasI AATT 4 cut(s) 151, 180, 246, 344
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TseFI GTSAC 2 cut(s) 422, 612
TseI GCWGC 8 cut(s) 26, 72, 93, 102, 109, 482, 497, 562
Tsp45I GTSAC 2 cut(s) 422, 612
TspDTI ATGAA 1 cut(s) 330
Tth111I GACNNNGTC 1 cut(s) 639
VpaK11BI GGWCC 1 cut(s) 635
XspI CTAG 5 cut(s) 53, 123, 128, 138, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.