Rmu_sc0003516.1_g000003

malic enzyme

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003516.1
N/A
Physical Location & Seq
Forward (+)
10576 .. 11252
677 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
atgatctcgctgaatagaagctgcttcctgagcaacgggggcatttctgggaattgtagcccgttctctcaggggcagaagaggcggtcggcttcgctgaaagtggtggctgtagggacgaacgccgccaggaaaggtgaacgaaacggcagcatagtgatggagaacgcagcaaaagaagttgttgagctgaaatctaccgtccatggtggggacaaagacgtgtatggtgaggatactgccagcgaggaccaacctgtcacgccctggagtgtgtctgttgcaaggtctgagcaaagagtaatttgctttactaatataatattgtctcttgtaatatcgtcatgtatgttgttcttgcaattctaa

Protein Analysis

122

Amino Acids

13.11

Weight (kDa)

8.43

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AciI CCGC 2 cut(s) 85, 126
AfiI CCNNNNNNNGG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 222
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 2 cut(s) 128, 266
AluBI AGCT 2 cut(s) 21, 190
AluI AGCT 2 cut(s) 21, 190
Alw26I GTCTC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 21, 150, 170
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 2 cut(s) 149, 242
AvaII GGWCC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 8, 162, 182
BccI CCATC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 130, 268
BciVI GTATCC 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 111
BfuI GTATCC 1 cut(s) 229
BisI GCNGC 4 cut(s) 22, 126, 151, 171
BlsI GCNGC 4 cut(s) 23, 127, 152, 172
Bme1390I CCNGG 2 cut(s) 130, 268
Bme18I GGWCC 1 cut(s) 250
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 130, 268
BpmI CTGGAG 1 cut(s) 289
Bpu10I CCTNAGC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 205, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 211
BseBI CCWGG 2 cut(s) 130, 268
BseDI CCNNGG 2 cut(s) 205, 266
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 3 cut(s) 20, 83, 282
BseXI GCAGC 3 cut(s) 8, 162, 182
Bsh1285I CGRYCG 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 89
BslFI GGGAC 2 cut(s) 130, 227
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 2 cut(s) 130, 227
Bsp143I GATC 1 cut(s) 3
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 2 cut(s) 85, 126
BspCNI CTCAG 3 cut(s) 21, 82, 283
BssECI CCNNGG 2 cut(s) 205, 266
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 130, 268
Bst4CI ACNGT 1 cut(s) 202
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 3 cut(s) 29, 69, 291
BstDSI CCRYGG 1 cut(s) 205
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 1 cut(s) 3
BstMCI CGRYCG 1 cut(s) 89
BstMWI GCNNNNNNNGC 3 cut(s) 30, 39, 82
BstNI CCWGG 2 cut(s) 130, 268
BstSCI CCNGG 2 cut(s) 128, 266
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 8, 162, 182
BsuI GTATCC 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 205
BtrI CACGTC 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 250
CviAII CATG 2 cut(s) 206, 345
CviJI RGCY 5 cut(s) 21, 60, 92, 110, 190
CviKI_1 RGCY 5 cut(s) 21, 60, 92, 110, 190
DdeI CTNAG 3 cut(s) 29, 69, 291
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 128, 266
EcoT14I CCWWGG 1 cut(s) 205
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 2 cut(s) 209, 348
FaiI YATR 6 cut(s) 155, 207, 228, 320, 346, 350
FaqI GGGAC 2 cut(s) 130, 227
FatI CATG 2 cut(s) 205, 344
Fnu4HI GCNGC 4 cut(s) 22, 126, 151, 171
Fsp4HI GCNGC 4 cut(s) 22, 126, 151, 171
GluI GCNGC 4 cut(s) 22, 126, 151, 171
GsuI CTGGAG 1 cut(s) 289
Hin1II CATG 2 cut(s) 209, 348
HphI GGTGA 2 cut(s) 149, 242
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 284, 361
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 39, 82
HpyF3I CTNAG 3 cut(s) 29, 69, 291
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 209, 348
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 9 cut(s) 33, 41, 56, 115, 142, 253, 256, 270, 280
Lsp1109I GCAGC 3 cut(s) 8, 162, 182
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 1 cut(s) 91
MluCI AATT 3 cut(s) 52, 303, 362
MnlI CCTC 3 cut(s) 75, 226, 241
MslI CAYNNNNRTG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 130, 268
MvaI CCWGG 2 cut(s) 130, 268
MwoI GCNNNNNNNGC 3 cut(s) 30, 39, 82
NcoI CCATGG 1 cut(s) 205
NdeII GATC 1 cut(s) 3
NlaIII CATG 2 cut(s) 209, 348
NmuCI GTSAC 1 cut(s) 259
PkrI GCNGC 4 cut(s) 23, 127, 152, 172
Psp6I CCWGG 2 cut(s) 128, 266
PspGI CCWGG 2 cut(s) 128, 266
PspPI GGNCC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 158
SatI GCNGC 4 cut(s) 22, 126, 151, 171
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 130, 268
SetI ASST 6 cut(s) 23, 139, 192, 225, 259, 290
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 3 cut(s) 52, 303, 362
SsiI CCGC 2 cut(s) 85, 126
SspI AATATT 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 128, 266
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 225
TasI AATT 3 cut(s) 52, 303, 362
TauI GCSGC 1 cut(s) 128
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 21, 150, 170
Tsp45I GTSAC 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.