Rmu_sc0003649.1_g000025

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003649.1
N/A
Physical Location & Seq
Reverse (-)
112871 .. 113551
681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
atggcaacggtctctgctgtcttttgtgacagatcctgccgccccttttgtttcgtccctattgatttggatcacatgcgtggaaccggtagtactagtagtaatattaggaaccatggaaaccaaaacccatctgggtctggttcacctacttgtaatcctcttgattttgtacagaaccaatatgattatggcatcaaaactgtccacacctttcctgtcaatcgtcttcattttgatcacaaaagcccatctcgctctcgttcactccctttcaatcctcttacgtttgctcaaaaccaatatcatgatgacatcataattatccagtcctgcaatggcctcttcctgtgtattctttttcataataaactgaaccccccatatcttattttcaatcctacaacaaaccagttctctacacttattcctcctccaactactgctgttacctccgctactggtgaatcctgcaacggcctcttcttgtgccatcttttgagtgcaaaagaagtgaaccccccatattttattctcaatcccacaaccatccagttctccacagttattcctccagcagctactgctgttactaacgatgctggtcaacaagtagaaccctatatcatcagtattgctttggcttttgacccttccaaatcacctcattcgcaacaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

226

Amino Acids

24.86

Weight (kDa)

6.73

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 40, 456
AclWI GGATC 2 cut(s) 27, 78
AfaI GTAC 2 cut(s) 94, 174
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 2 cut(s) 277, 397
AhlI ACTAGT 1 cut(s) 95
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 1 cut(s) 581
AluI AGCT 1 cut(s) 581
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 2 cut(s) 27, 78
AlwNI CAGNNNCTG 2 cut(s) 36, 584
AoxI GGCC 2 cut(s) 340, 478
ApeKI GCWGC 1 cut(s) 578
AsiGI ACCGGT 1 cut(s) 86
AsuHPI GGTGA 3 cut(s) 138, 476, 654
BbsI GAAGAC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 590
BccI CCATC 4 cut(s) 139, 259, 501, 557
BceAI ACGGC 1 cut(s) 493
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 16
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 1 cut(s) 96
BisI GCNGC 2 cut(s) 40, 579
BlsI GCNGC 2 cut(s) 41, 580
BmcAI AGTACT 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 85, 113
BmsI GCATC 2 cut(s) 204, 589
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 115
BsaWI WCCGGW 1 cut(s) 86
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 4 cut(s) 328, 412, 466, 553
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 69
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 1 cut(s) 549
BseJI GATNNNNATC 1 cut(s) 69
BseMI GCAATG 1 cut(s) 343
BseNI ACTGG 4 cut(s) 328, 412, 466, 553
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 1 cut(s) 590
BshFI GGCC 2 cut(s) 342, 480
BshTI ACCGGT 1 cut(s) 86
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 2 cut(s) 342, 480
Bso31I GGTCTC 1 cut(s) 16
Bsp1407I TGTACA 1 cut(s) 172
Bsp143I GATC 3 cut(s) 32, 70, 238
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 2 cut(s) 40, 456
BspANI GGCC 2 cut(s) 342, 480
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 2 cut(s) 85, 113
BspPI GGATC 2 cut(s) 27, 78
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 172
BsrI ACTGG 4 cut(s) 328, 412, 466, 553
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 3 cut(s) 32, 70, 238
BssT1I CCWWGG 1 cut(s) 115
Bst4CI ACNGT 3 cut(s) 10, 205, 565
Bst6I CTCTTC 2 cut(s) 350, 488
BstAPI GCANNNNNTGC 1 cut(s) 584
BstAUI TGTACA 1 cut(s) 172
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 1 cut(s) 549
BstKTI GATC 3 cut(s) 35, 73, 241
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 3 cut(s) 32, 70, 238
BstMWI GCNNNNNNNGC 2 cut(s) 255, 584
BstNSI RCATGY 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 590
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 2 cut(s) 342, 480
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 1 cut(s) 549
CaiI CAGNNNCTG 2 cut(s) 36, 584
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 86
Csp6I GTAC 2 cut(s) 93, 173
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 3 cut(s) 76, 116, 308
CviJI RGCY 5 cut(s) 249, 342, 480, 581, 644
CviKI_1 RGCY 5 cut(s) 249, 342, 480, 581, 644
CviQI GTAC 2 cut(s) 93, 173
DpnI GATC 3 cut(s) 34, 72, 240
DpnII GATC 3 cut(s) 32, 70, 238
Eam1104I CTCTTC 2 cut(s) 350, 488
EarI CTCTTC 2 cut(s) 350, 488
Eco130I CCWWGG 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 3 cut(s) 79, 119, 311
FaqI GGGAC 1 cut(s) 41
FatI CATG 3 cut(s) 75, 115, 307
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 40, 579
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 40, 579
FspBI CTAG 1 cut(s) 96
GluI GCNGC 2 cut(s) 40, 579
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 342, 480
HapII CCGG 1 cut(s) 87
Hin1II CATG 3 cut(s) 79, 119, 311
HincII GTYRAC 1 cut(s) 608
HindII GTYRAC 1 cut(s) 608
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 87
HphI GGTGA 3 cut(s) 138, 476, 654
Hpy166II GTNNAC 5 cut(s) 146, 208, 266, 517, 608
Hpy188III TCNNGA 2 cut(s) 164, 308
Hpy8I GTNNAC 5 cut(s) 146, 208, 266, 517, 608
HpyAV CCTTC 1 cut(s) 663
HpyCH4III ACNGT 3 cut(s) 10, 205, 565
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 3 cut(s) 336, 474, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 255, 584
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 3 cut(s) 79, 119, 311
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 3 cut(s) 32, 70, 238
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 2 cut(s) 204, 589
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 3 cut(s) 26, 448, 589
MalI GATC 3 cut(s) 34, 72, 240
MboI GATC 3 cut(s) 32, 70, 238
MboII GAAGA 3 cut(s) 221, 337, 475
MflI RGATCY 1 cut(s) 32
MluCI AATT 1 cut(s) 321
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 9 cut(s) 171, 291, 353, 441, 444, 463, 491, 582, 675
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 255, 584
NcoI CCATGG 1 cut(s) 115
NdeII GATC 3 cut(s) 32, 70, 238
NlaIII CATG 3 cut(s) 79, 119, 311
NlaIV GGNNCC 2 cut(s) 85, 113
NmuCI GTSAC 1 cut(s) 26
NspI RCATGY 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 78
PagI TCATGA 1 cut(s) 307
PfeI GAWTC 1 cut(s) 467
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 2 cut(s) 41, 580
PspN4I GGNNCC 2 cut(s) 85, 113
PsrI GAACNNNNNNTAC 2 cut(s) 76, 108
PstNI CAGNNNCTG 2 cut(s) 36, 584
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 2 cut(s) 94, 174
RsaNI GTAC 2 cut(s) 93, 173
RseI CAYNNNNRTG 1 cut(s) 78
SatI GCNGC 2 cut(s) 40, 579
Sau3AI GATC 3 cut(s) 32, 70, 238
ScaI AGTACT 1 cut(s) 94
SetI ASST 6 cut(s) 151, 215, 290, 455, 583, 667
SfaNI GCATC 2 cut(s) 204, 589
SmiMI CAYNNNNRTG 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 1 cut(s) 321
SsiI CCGC 2 cut(s) 40, 456
SspI AATATT 1 cut(s) 106
SspMI CTAG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 3 cut(s) 10, 205, 565
TaiI ACGT 1 cut(s) 290
TasI AATT 1 cut(s) 321
TatI WGTACW 2 cut(s) 92, 172
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 1 cut(s) 467
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 221, 353
XceI RCATGY 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 3 cut(s) 131, 188, 335
XspI CTAG 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.