Rmu_sc0003784.1_g000022

5-formyltetrahydrofolate cyclo-ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003784.1
N/A
Physical Location & Seq
Reverse (-)
80629 .. 84862
4234 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
atggctctaggagaccgaaagattcaaacaaaaggattggagcagctgtcttatcccttgcaaatcagactcactgatctcaaccaaagtaagagtatatgtgcttatataagctctcctgagctgcgagaagtagatacttcaaggattgtgtcagaaattctatcgaattcgggcaatggtcaagaaggtgatgtgctgatcaggagaaagctttacgttccgcgggttgaggataggaatagtaatatgcgcatgctgaggattaacagtgttggtgatctgattgtgaagtctatgaacgttttggaacctgcgctttcggatgccgaggggaaacaatatgaagatgctatggaggcaaaggatccagttgacttgttcatcctacctggtcttgcttttgacagatgtggaagacgtttaggccgtagtggaggttattatgacatgttcctaaagaggtaccaaaagctcgcaaaagatcggaaatggaaggatcccctctgtggtaaatactcactattgttattgcgctatcatattcaatacagatcgtggaggaaggagccatacctgtcacttcgaatgatgtttctgtggatgctcttgtatccccagctggttttgttccaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
5-FTHF_cyc-lig

Protein Analysis

212

Amino Acids

24.75

Weight (kDa)

9.45

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 23 - 162 1.2e-16 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 322
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 1 cut(s) 465
AccII CGCG 1 cut(s) 226
AciI CCGC 2 cut(s) 224, 226
AclI AACGTT 1 cut(s) 303
AclWI GGATC 4 cut(s) 362, 375, 494, 507
AcsI RAATTY 2 cut(s) 159, 169
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 3 cut(s) 26, 144, 548
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 6 cut(s) 46, 114, 124, 214, 475, 622
AluI AGCT 6 cut(s) 46, 114, 124, 214, 475, 622
Alw26I GTCTC 1 cut(s) 6
AlwI GGATC 4 cut(s) 362, 375, 494, 507
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 2 cut(s) 43, 124
ApoI RAATTY 2 cut(s) 159, 169
Asp718I GGTACC 1 cut(s) 465
AspLEI GCGC 3 cut(s) 255, 319, 537
AsuHPI GGTGA 2 cut(s) 203, 290
AsuII TTCGAA 1 cut(s) 586
BamHI GGATCC 2 cut(s) 367, 499
BanI GGYRCC 1 cut(s) 465
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BbsI GAAGAC 1 cut(s) 424
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 55, 111
BceAI ACGGC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 393
BciVI GTATCC 1 cut(s) 624
BclI TGATCA 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 6
BfaI CTAG 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 322
BfuI GTATCC 1 cut(s) 624
BisI GCNGC 2 cut(s) 44, 125
BlsI GCNGC 2 cut(s) 45, 126
Bme1390I CCNGG 1 cut(s) 393
BmiI GGNNCC 5 cut(s) 312, 369, 467, 501, 570
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 3 cut(s) 316, 340, 594
BpiI GAAGAC 1 cut(s) 424
Bpu10I CCTNAGC 2 cut(s) 120, 260
Bpu14I TTCGAA 1 cut(s) 586
BsaI GGTCTC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 224, 330
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 371
Bse3DI GCAATG 1 cut(s) 184
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 224, 330
BseGI GGATG 3 cut(s) 331, 384, 609
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMI GCAATG 1 cut(s) 184
BseMII CTCAG 2 cut(s) 111, 251
BseNI ACTGG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 55, 111
BseYI CCCAGC 1 cut(s) 618
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 1 cut(s) 429
BshNI GGYRCC 1 cut(s) 465
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 6
BsnI GGCC 1 cut(s) 429
Bso31I GGTCTC 1 cut(s) 6
Bsp119I TTCGAA 1 cut(s) 586
Bsp143I GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
BspACI CCGC 2 cut(s) 224, 226
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 112, 252
BspFNI CGCG 1 cut(s) 226
BspLI GGNNCC 5 cut(s) 312, 369, 467, 501, 570
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 4 cut(s) 362, 375, 494, 507
BspT104I TTCGAA 1 cut(s) 586
BspT107I GGYRCC 1 cut(s) 465
BspTNI GGTCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 184
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 224, 330
BssMI GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 257, 477
BstDEI CTNAG 2 cut(s) 120, 260
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 3 cut(s) 331, 384, 609
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 3 cut(s) 255, 319, 537
BstKTI GATC 7 cut(s) 79, 204, 283, 370, 487, 502, 557
BstMAI GTCTC 1 cut(s) 6
BstMBI GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 2 cut(s) 259, 454
BstSCI CCNGG 1 cut(s) 391
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 55, 111
BstV2I GAAGAC 1 cut(s) 424
BstX2I RGATCY 2 cut(s) 367, 499
BstYI RGATCY 2 cut(s) 367, 499
BsuI GTATCC 1 cut(s) 624
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 224
BtsCI GGATG 3 cut(s) 331, 384, 609
BtsIMutI CAGTG 2 cut(s) 72, 277
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 257, 477
CfoI GCGC 3 cut(s) 255, 319, 537
Cfr42I CCGCGG 1 cut(s) 227
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 1 cut(s) 466
CviAII CATG 2 cut(s) 256, 451
CviJI RGCY 9 cut(s) 5, 46, 114, 124, 214, 429, 475, 571, 622
CviKI_1 RGCY 9 cut(s) 5, 46, 114, 124, 214, 429, 475, 571, 622
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 2 cut(s) 120, 260
DpnI GATC 7 cut(s) 78, 203, 282, 369, 486, 501, 556
DpnII GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
Eco31I GGTCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 391
FaeI CATG 2 cut(s) 259, 454
FatI CATG 2 cut(s) 255, 450
FauI CCCGC 1 cut(s) 219
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 44, 125
FokI GGATG 3 cut(s) 338, 371, 616
Fsp4HI GCNGC 2 cut(s) 44, 125
FspAI RTGCGCAY 1 cut(s) 254
FspBI CTAG 1 cut(s) 8
FspI TGCGCA 1 cut(s) 254
GlaI GCGC 3 cut(s) 254, 318, 536
GluI GCNGC 2 cut(s) 44, 125
GsaI CCCAGC 1 cut(s) 622
HaeIII GGCC 1 cut(s) 429
HhaI GCGC 3 cut(s) 255, 319, 537
Hin1II CATG 2 cut(s) 259, 454
Hin6I GCGC 3 cut(s) 253, 317, 535
HinP1I GCGC 3 cut(s) 253, 317, 535
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 2 cut(s) 22, 69
HphI GGTGA 2 cut(s) 203, 290
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 5 cut(s) 68, 157, 285, 325, 489
Hpy188III TCNNGA 3 cut(s) 119, 185, 205
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 3 cut(s) 182, 490, 559
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 3 cut(s) 219, 303, 421
HpyCH4V TGCA 1 cut(s) 61
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 2 cut(s) 120, 260
HpySE526I ACGT 3 cut(s) 219, 303, 421
Hsp92II CATG 2 cut(s) 259, 454
HspAI GCGC 3 cut(s) 253, 317, 535
KpnI GGTACC 1 cut(s) 469
Ksp22I TGATCA 1 cut(s) 201
KspI CCGCGG 1 cut(s) 227
Kzo9I GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
LmnI GCTCC 2 cut(s) 40, 568
LpnPI CCDG 9 cut(s) 132, 190, 327, 378, 384, 405, 590, 608, 632
Lsp1109I GCAGC 2 cut(s) 55, 111
LweI GCATC 3 cut(s) 316, 340, 594
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 1 cut(s) 8
MaeII ACGT 3 cut(s) 219, 303, 421
MaeIII GTNAC 1 cut(s) 579
MalI GATC 7 cut(s) 78, 203, 282, 369, 486, 501, 556
MboI GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
MboII GAAGA 2 cut(s) 359, 429
MflI RGATCY 2 cut(s) 367, 499
MluCI AATT 2 cut(s) 159, 169
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 8 cut(s) 226, 255, 325, 352, 431, 456, 515, 555
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 3 cut(s) 46, 226, 622
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
NlaIII CATG 2 cut(s) 259, 454
NlaIV GGNNCC 5 cut(s) 312, 369, 467, 501, 570
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 579
NsbI TGCGCA 1 cut(s) 254
NspI RCATGY 2 cut(s) 259, 454
NspV TTCGAA 1 cut(s) 586
PaeI GCATGC 1 cut(s) 259
PciI ACATGT 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 427
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 2 cut(s) 45, 126
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PscI ACATGT 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 391
PspFI CCCAGC 1 cut(s) 618
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 5 cut(s) 312, 369, 467, 501, 570
PsuI RGATCY 2 cut(s) 367, 499
PvuII CAGCTG 2 cut(s) 46, 622
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
SacII CCGCGG 1 cut(s) 227
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 44, 125
Sau3AI GATC 7 cut(s) 76, 201, 280, 367, 484, 499, 554
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 393
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 3 cut(s) 316, 340, 594
Sfr303I CCGCGG 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 227
SphI GCATGC 1 cut(s) 259
Sse9I AATT 2 cut(s) 159, 169
SsiI CCGC 2 cut(s) 224, 226
SspMI CTAG 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 3 cut(s) 222, 306, 424
TaqI TCGA 2 cut(s) 167, 586
TaqII GACCGA 1 cut(s) 30
TasI AATT 2 cut(s) 159, 169
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 79, 277
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 2 cut(s) 43, 124
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 3 cut(s) 314, 360, 373
TspRI CASTG 2 cut(s) 79, 277
XapI RAATTY 2 cut(s) 159, 169
XceI RCATGY 2 cut(s) 259, 454
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.