Rmu_sc0003805.1_g000018

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003805.1
N/A
Physical Location & Seq
Forward (+)
77433 .. 77921
489 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
atggccaagggagaaaaggtcatttctaaaggtccctcgccaccagatctttcctctgtacaagttgaagtcttacttggaccttttggagattcgcctccaccacatacaaagtttggagatatgaaacaagatatcattataccaaaaagtggttcattcagaaagggagatttgaatagaccaaatgccacattttggagtgcaaacccaaactatgacttgttgacagaaggcacatatgctgtggtagagatgcttcaaggggataactgggttccagtttatgatgatgatgatttctccgtggttttcaagtggaatagagatgacaagggattatatagtgcagcaaccatagaatgggaaataccaatttatgcaaatttgggggtgtacaggctcaggcattttggttcatcaaggaaaaagaaggactcccccatcatctacttcactggtgcgtctagtgggtttgcagtgtcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04071 map00600 map01100 map04071
Pfam Domains
Protein Families
Ceramidase_alk Ceramidse_alk_C

Protein Analysis

162

Amino Acids

17.98

Weight (kDa)

5.66

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidse_alk_C PF17048 2 - 161 1.3e-40 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 152, 198, 363
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 385
AfaI GTAC 2 cut(s) 60, 398
AfiI CCNNNNNNNGG 3 cut(s) 152, 198, 363
AgsI TTSAA 4 cut(s) 68, 178, 263, 316
AjuI GAANNNNNNNTTGG 4 cut(s) 60, 92, 139, 171
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 32, 80
AvaII GGWCC 2 cut(s) 32, 80
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 243, 275
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 452
BfaI CTAG 1 cut(s) 468
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 352
Bme18I GGWCC 2 cut(s) 32, 80
BmgT120I GGNCC 2 cut(s) 32, 80
BmiI GGNNCC 2 cut(s) 34, 279
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 283
Bpu10I CCTNAGC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 6, 306
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 198, 363
Bse1I ACTGG 3 cut(s) 278, 281, 463
BseDI CCNNGG 2 cut(s) 6, 306
BseLI CCNNNNNNNGG 3 cut(s) 152, 198, 363
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 3 cut(s) 278, 281, 463
BseXI GCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 152, 198, 363
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 5
Bsp1407I TGTACA 2 cut(s) 58, 396
Bsp143I GATC 1 cut(s) 46
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 34, 279
BsrGI TGTACA 2 cut(s) 58, 396
BsrI ACTGG 3 cut(s) 278, 281, 463
BssECI CCNNGG 2 cut(s) 6, 306
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 6
BstAUI TGTACA 2 cut(s) 58, 396
BstDEI CTNAG 1 cut(s) 404
BstDSI CCRYGG 1 cut(s) 306
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 362
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 456, 486
Cfr13I GGNCC 2 cut(s) 32, 80
CseI GACGC 1 cut(s) 453
Csp6I GTAC 2 cut(s) 59, 397
CviJI RGCY 2 cut(s) 5, 403
CviKI_1 RGCY 2 cut(s) 5, 403
CviQI GTAC 2 cut(s) 59, 397
DdeI CTNAG 1 cut(s) 404
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 6
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 2 cut(s) 32, 80
EcoO109I RGGNCCY 1 cut(s) 32
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FaqI GGGAC 1 cut(s) 18
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 351
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 1 cut(s) 468
GluI GCNGC 1 cut(s) 351
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 453
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 2 cut(s) 92, 437
Hpy166II GTNNAC 2 cut(s) 228, 397
Hpy188I TCNGA 1 cut(s) 164
Hpy8I GTNNAC 2 cut(s) 228, 397
HpyAV CCTTC 2 cut(s) 227, 427
HpyCH4V TGCA 4 cut(s) 206, 350, 383, 479
HpyF3I CTNAG 1 cut(s) 404
Kzo9I GATC 1 cut(s) 46
LpnPI CCDG 6 cut(s) 57, 259, 294, 385, 391, 444
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 246
MaeI CTAG 1 cut(s) 468
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MflI RGATCY 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 375, 385
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 431
MnlI CCTC 3 cut(s) 46, 64, 108
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
NdeI CATATG 1 cut(s) 241
NdeII GATC 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 34, 279
PfeI GAWTC 1 cut(s) 92
PflMI CCANNNNNTGG 3 cut(s) 152, 198, 363
PkrI GCNGC 1 cut(s) 352
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 32
Psp5II RGGWCCY 1 cut(s) 32
PspN4I GGNNCC 2 cut(s) 34, 279
PspPI GGNCC 2 cut(s) 32, 80
PspPPI RGGWCCY 1 cut(s) 32
PsuI RGATCY 1 cut(s) 46
RsaI GTAC 2 cut(s) 60, 398
RsaNI GTAC 2 cut(s) 59, 397
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 2 cut(s) 32, 80
SchI GAGTC 1 cut(s) 431
SetI ASST 3 cut(s) 21, 34, 85
SfaNI GCATC 1 cut(s) 246
SinI GGWCC 2 cut(s) 32, 80
Sse9I AATT 2 cut(s) 375, 385
SspMI CTAG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 6
TasI AATT 2 cut(s) 375, 385
TatI WGTACW 2 cut(s) 58, 396
TfiI GAWTC 1 cut(s) 92
TscAI CASTG 2 cut(s) 463, 486
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 3 cut(s) 140, 147, 408
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 2 cut(s) 463, 486
Van91I CCANNNNNTGG 3 cut(s) 152, 198, 363
VpaK11BI GGWCC 2 cut(s) 32, 80
XapI RAATTY 1 cut(s) 385
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.