Rmu_sc0003848.1_g000004

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003848.1
N/A
Physical Location & Seq
Reverse (-)
8805 .. 9422
618 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
atggactcaattcgatcaaacttaggttttaccagctgtattgcatatccatgtgaggatgactctaagggcgtggcactactctggagagcggagactccagtgcgattgaaaatgtatggaccgcatgatattgatgtagaggtgggccaacttgggtcgcaagaaatctggcgattcactagtatctatggttttgcacagcgcgattttaatgagatcttaaccaatgctaacaaatcgggaggaatccccagagctatggtacctatgatgttatttaggaaaactatgaaggcttgtgacctgcatgatatgggatttgttggaggcagatttacctggtttgatagatataccaaggaaagactagatcgctgctttcaatctccacaatggcttgcttggtatccttggtcgagggttttaacattgccgccaattgaatctgatcattccccactattgattgaagtgaatgctgagagaagattgcaatggagaactccacgcagattcaggtttgaggaaatgtggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

179

Amino Acids

21.13

Weight (kDa)

8.39

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 315
Acc65I GGTACC 1 cut(s) 265
AccB1I GGYRCC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 92
AccII CGCG 1 cut(s) 207
AciI CCGC 3 cut(s) 92, 125, 437
AfaI GTAC 1 cut(s) 267
AgsI TTSAA 4 cut(s) 112, 386, 446, 473
AhlI ACTAGT 1 cut(s) 182
AjnI CCWGG 1 cut(s) 341
AluBI AGCT 2 cut(s) 36, 260
AluI AGCT 2 cut(s) 36, 260
Alw26I GTCTC 1 cut(s) 89
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 378
Asp718I GGTACC 1 cut(s) 265
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 122, 148
AvaII GGWCC 1 cut(s) 122
BanI GGYRCC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 343
BciVI GTATCC 1 cut(s) 420
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 89
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 2 cut(s) 183, 371
BfuAI ACCTGC 1 cut(s) 315
BfuI GTATCC 1 cut(s) 420
BglII AGATCT 1 cut(s) 219
BisI GCNGC 2 cut(s) 379, 437
BlsI GCNGC 2 cut(s) 380, 438
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 122, 148
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 1 cut(s) 343
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpmI CTGGAG 2 cut(s) 84, 106
BsaJI CCNNGG 2 cut(s) 360, 413
Bse1I ACTGG 1 cut(s) 101
Bse3DI GCAATG 2 cut(s) 431, 503
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 2 cut(s) 360, 413
BseGI GGATG 1 cut(s) 64
BseMI GCAATG 2 cut(s) 431, 503
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 101
BseXI GCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 14, 219, 373, 451
BspACI CCGC 3 cut(s) 92, 125, 437
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 475
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 267
BspMI ACCTGC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 265
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 431, 503
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 360, 413
BssMI GATC 4 cut(s) 14, 219, 373, 451
BssT1I CCWWGG 2 cut(s) 360, 413
Bst2UI CCWGG 1 cut(s) 343
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 3 cut(s) 22, 66, 483
BstF5I GGATG 1 cut(s) 64
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 4 cut(s) 17, 222, 376, 454
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 4 cut(s) 14, 219, 373, 451
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 341
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 219
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 150
BtsCI GGATG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 402
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 122, 148
CsiI ACCWGGT 1 cut(s) 341
Csp6I GTAC 1 cut(s) 266
CviAII CATG 3 cut(s) 51, 128, 311
CviJI RGCY 5 cut(s) 36, 150, 260, 299, 400
CviKI_1 RGCY 5 cut(s) 36, 150, 260, 299, 400
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 3 cut(s) 22, 66, 483
DpnI GATC 4 cut(s) 16, 221, 375, 453
DpnII GATC 4 cut(s) 14, 219, 373, 451
Eco130I CCWWGG 2 cut(s) 360, 413
Eco47I GGWCC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 2 cut(s) 360, 413
ErhI CCWWGG 2 cut(s) 360, 413
FaeI CATG 3 cut(s) 54, 131, 314
FatI CATG 3 cut(s) 50, 127, 310
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 379, 437
FokI GGATG 1 cut(s) 71
Fsp4HI GCNGC 2 cut(s) 379, 437
FspBI CTAG 2 cut(s) 183, 371
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 379, 437
GsuI CTGGAG 2 cut(s) 84, 106
HaeIII GGCC 1 cut(s) 150
HhaI GCGC 1 cut(s) 207
Hin1II CATG 3 cut(s) 54, 131, 314
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 7 cut(s) 5, 62, 97, 177, 249, 446, 516
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 2 cut(s) 85, 243
HpyAV CCTTC 1 cut(s) 289
HpyCH4V TGCA 4 cut(s) 44, 200, 310, 496
HpyF3I CTNAG 3 cut(s) 22, 66, 483
Hsp92II CATG 3 cut(s) 54, 131, 314
HspAI GCGC 1 cut(s) 205
KpnI GGTACC 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 4 cut(s) 14, 219, 373, 451
LpnPI CCDG 9 cut(s) 46, 70, 114, 157, 268, 320, 328, 355, 505
Lsp1109I GCAGC 1 cut(s) 365
MabI ACCWGGT 1 cut(s) 341
MaeI CTAG 2 cut(s) 183, 371
MaeIII GTNAC 1 cut(s) 302
MalI GATC 4 cut(s) 16, 221, 375, 453
MbiI CCGCTC 1 cut(s) 92
MboI GATC 4 cut(s) 14, 219, 373, 451
MboII GAAGA 1 cut(s) 501
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 219
MluCI AATT 2 cut(s) 9, 441
MlyI GAGTC 2 cut(s) 56, 91
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 6 cut(s) 49, 136, 239, 323, 414, 520
MseI TTAA 3 cut(s) 213, 224, 428
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 1 cut(s) 343
MvnI CGCG 1 cut(s) 207
NdeII GATC 4 cut(s) 14, 219, 373, 451
NlaIII CATG 3 cut(s) 54, 131, 314
NlaIV GGNNCC 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 302
PctI GAATGC 1 cut(s) 484
PfeI GAWTC 4 cut(s) 177, 249, 446, 516
PkrI GCNGC 2 cut(s) 380, 438
PleI GAGTC 2 cut(s) 56, 91
PpsI GAGTC 2 cut(s) 56, 91
Psp6I CCWGG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 2 cut(s) 122, 148
PsuI RGATCY 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 49
SaqAI TTAA 3 cut(s) 213, 224, 428
SatI GCNGC 2 cut(s) 379, 437
Sau3AI GATC 4 cut(s) 14, 219, 373, 451
Sau96I GGNCC 2 cut(s) 122, 148
SchI GAGTC 2 cut(s) 56, 91
ScrFI CCNGG 1 cut(s) 343
SetI ASST 8 cut(s) 28, 38, 147, 262, 271, 309, 344, 524
SexAI ACCWGGT 1 cut(s) 341
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 49
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 2 cut(s) 9, 441
SsiI CCGC 3 cut(s) 92, 125, 437
SspMI CTAG 2 cut(s) 183, 371
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 2 cut(s) 360, 413
TaqI TCGA 2 cut(s) 13, 419
TasI AATT 2 cut(s) 9, 441
TauI GCSGC 1 cut(s) 439
TfiI GAWTC 4 cut(s) 177, 249, 446, 516
Tru1I TTAA 3 cut(s) 213, 224, 428
Tru9I TTAA 3 cut(s) 213, 224, 428
TscAI CASTG 1 cut(s) 108
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 378
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 308
TspRI CASTG 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 122
XspI CTAG 2 cut(s) 183, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.