Rmu_sc0003848.1_g000010

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003848.1
N/A
Physical Location & Seq
Forward (+)
28700 .. 31062
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
atgagttcttctctggcgaaagctaatgcctctccacatctcctccgtcaaacctccagtcaacgagctttcgtctttggagatcagatcacctgtgctttagagctccacctcgctcagccgaaacgacaagcttatttcggttcctcgccatgctggactcgagaaagacgacctacagtgccgtgcgagcaactactcaccagagactccatcatggagcgagccgggctcgaagactcgggaggcccaaagacccactgcctaaaccctacgttcccgcaccccgccggccaaatctggaacctccaagaagcccgtcagccaccgcctagtcaatccggctcgctacaatggtctgacccctgcaagcacgaaccaaaagccttatcagcgccagagcaccctgctgcagacgggatcggagcaccgctgcactgctacgcgaaatctagaaggtttttgcatgcagtcattgaaactgattgtctcttggcagttcaagacttggctcggactgatgtgaatctgtcggaactcagtaatctagttcttgacatacaacaagcactagaagatatgcagggtgtccaaatcagttttgcgtcccggtcctgcaacaaagtggcgcacagactagcaatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

215

Amino Acids

23.64

Weight (kDa)

7.1

Isoelectric Point (pI)

66.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 149 - 214 1.8e-08 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 446
AciI CCGC 4 cut(s) 281, 288, 329, 431
AclWI GGATC 1 cut(s) 428
AcoI YGGCCR 1 cut(s) 292
AgsI TTSAA 2 cut(s) 479, 503
AluBI AGCT 4 cut(s) 23, 68, 106, 134
AluI AGCT 4 cut(s) 23, 68, 106, 134
Alw21I GWGCWC 3 cut(s) 108, 405, 430
Alw26I GTCTC 2 cut(s) 201, 494
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 2 cut(s) 162, 241
AoxI GGCC 2 cut(s) 247, 292
ApeKI GCWGC 2 cut(s) 410, 433
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
AspLEI GCGC 2 cut(s) 397, 631
AspS9I GGNCC 2 cut(s) 248, 612
AsuC2I CCSGG 2 cut(s) 229, 610
AsuHPI GGTGA 2 cut(s) 82, 193
AvaI CYCGRG 2 cut(s) 162, 241
AvaII GGWCC 1 cut(s) 612
BanII GRGCYC 2 cut(s) 108, 234
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 3 cut(s) 108, 405, 430
BbvI GCAGC 2 cut(s) 397, 420
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 169
BcnI CCSGG 2 cut(s) 229, 610
BcoDI GTCTC 2 cut(s) 201, 494
BfaI CTAG 6 cut(s) 333, 453, 548, 572, 638, 646
BfmI CTRYAG 2 cut(s) 177, 411
BfoI RGCGCY 1 cut(s) 398
BisI GCNGC 2 cut(s) 411, 434
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 2 cut(s) 412, 435
Bme1390I CCNGG 2 cut(s) 229, 610
Bme18I GGWCC 1 cut(s) 612
BmeT110I CYCGRG 2 cut(s) 162, 241
BmgT120I GGNCC 2 cut(s) 248, 612
BmiI GGNNCC 2 cut(s) 145, 305
BmrFI CCNGG 2 cut(s) 229, 610
BpiI GAAGAC 1 cut(s) 243
BplI GAGNNNNNCTC 3 cut(s) 27, 216, 248
BpmI CTGGAG 1 cut(s) 40
Bpu1102I GCTNAGC 1 cut(s) 117
BpuMI CCSGG 2 cut(s) 229, 610
BsaBI GATNNNNATC 1 cut(s) 525
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 525
BseJI GATNNNNATC 1 cut(s) 525
BseMII CTCAG 2 cut(s) 131, 553
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 397, 420
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 2 cut(s) 249, 294
BsiHKAI GWGCWC 3 cut(s) 108, 405, 430
BsiHKCI CYCGRG 2 cut(s) 162, 241
BsiSI CCGG 4 cut(s) 228, 291, 342, 610
BslFI GGGAC 1 cut(s) 592
BsmAI GTCTC 2 cut(s) 201, 494
BsmFI GGGAC 1 cut(s) 592
BsnI GGCC 2 cut(s) 249, 294
BsoBI CYCGRG 2 cut(s) 162, 241
Bsp1286I GDGCHC 4 cut(s) 108, 234, 405, 430
Bsp143I GATC 3 cut(s) 82, 87, 420
Bsp1720I GCTNAGC 1 cut(s) 117
BspACI CCGC 4 cut(s) 281, 288, 329, 431
BspANI GGCC 2 cut(s) 249, 294
BspCNI CTCAG 2 cut(s) 130, 552
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 2 cut(s) 145, 305
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 1 cut(s) 428
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 57
BssAI RCCGGY 1 cut(s) 290
BssMI GATC 3 cut(s) 82, 87, 420
Bst4CI ACNGT 1 cut(s) 181
BstC8I GCNNGC 6 cut(s) 191, 225, 292, 347, 371, 468
BstDEI CTNAG 2 cut(s) 117, 539
BstFNI CGCG 1 cut(s) 446
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 2 cut(s) 397, 631
BstKTI GATC 3 cut(s) 85, 90, 423
BstMAI GTCTC 2 cut(s) 201, 494
BstMBI GATC 3 cut(s) 82, 87, 420
BstMWI GCNNNNNNNGC 3 cut(s) 190, 229, 392
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 227, 608
BstSFI CTRYAG 2 cut(s) 177, 411
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 2 cut(s) 397, 420
BstV2I GAAGAC 1 cut(s) 243
BsuRI GGCC 2 cut(s) 249, 294
BtsI GCAGTG 2 cut(s) 259, 436
BtsIMutI CAGTG 3 cut(s) 186, 259, 436
Cac8I GCNNGC 6 cut(s) 191, 225, 292, 347, 371, 468
CfoI GCGC 2 cut(s) 397, 631
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 2 cut(s) 248, 612
CseI GACGC 1 cut(s) 594
CviAII CATG 3 cut(s) 153, 217, 467
DdeI CTNAG 2 cut(s) 117, 539
DpnI GATC 3 cut(s) 84, 89, 422
DpnII GATC 3 cut(s) 82, 87, 420
EaeI YGGCCR 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 108, 234
Eco47I GGWCC 1 cut(s) 612
Eco53kI GAGCTC 1 cut(s) 106
Eco88I CYCGRG 2 cut(s) 162, 241
EcoICRI GAGCTC 1 cut(s) 106
EcoT38I GRGCYC 2 cut(s) 108, 234
FaeI CATG 3 cut(s) 156, 220, 470
FaiI YATR 5 cut(s) 154, 218, 468, 560, 581
FaqI GGGAC 1 cut(s) 592
FatI CATG 3 cut(s) 152, 216, 466
FauI CCCGC 2 cut(s) 288, 295
Fnu4HI GCNGC 2 cut(s) 411, 434
FriOI GRGCYC 2 cut(s) 108, 234
Fsp4HI GCNGC 2 cut(s) 411, 434
FspBI CTAG 6 cut(s) 333, 453, 548, 572, 638, 646
GlaI GCGC 2 cut(s) 396, 630
GluI GCNGC 2 cut(s) 411, 434
GsuI CTGGAG 1 cut(s) 40
HaeII RGCGCY 1 cut(s) 398
HaeIII GGCC 2 cut(s) 249, 294
HapII CCGG 4 cut(s) 228, 291, 342, 610
HgaI GACGC 1 cut(s) 594
HhaI GCGC 2 cut(s) 397, 631
Hin1II CATG 3 cut(s) 156, 220, 470
Hin6I GCGC 2 cut(s) 395, 629
HinP1I GCGC 2 cut(s) 395, 629
HincII GTYRAC 1 cut(s) 62
HindII GTYRAC 1 cut(s) 62
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 4 cut(s) 160, 209, 239, 526
HpaII CCGG 4 cut(s) 228, 291, 342, 610
HphI GGTGA 2 cut(s) 82, 193
Hpy166II GTNNAC 1 cut(s) 62
Hpy188I TCNGA 5 cut(s) 87, 361, 425, 516, 535
Hpy188III TCNNGA 6 cut(s) 164, 243, 301, 453, 503, 554
Hpy8I GTNNAC 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 450
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 7 cut(s) 369, 413, 436, 466, 470, 583, 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 229, 392
HpyF3I CTNAG 2 cut(s) 117, 539
HpySE526I ACGT 1 cut(s) 275
Hsp92II CATG 3 cut(s) 156, 220, 470
HspAI GCGC 2 cut(s) 395, 629
KroI GCCGGC 1 cut(s) 290
KroNI GCCGGC 1 cut(s) 292
Kzo9I GATC 3 cut(s) 82, 87, 420
LmnI GCTCC 3 cut(s) 111, 220, 425
Lsp1109I GCAGC 2 cut(s) 397, 420
MaeI CTAG 6 cut(s) 333, 453, 548, 572, 638, 646
MaeII ACGT 1 cut(s) 275
MalI GATC 3 cut(s) 84, 89, 422
MboI GATC 3 cut(s) 82, 87, 420
MboII GAAGA 2 cut(s) 248, 587
MhlI GDGCHC 4 cut(s) 108, 234, 405, 430
MlyI GAGTC 3 cut(s) 154, 203, 233
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 7 cut(s) 40, 53, 64, 122, 157, 239, 317
MroNI GCCGGC 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 4 cut(s) 228, 291, 342, 610
MspR9I CCNGG 2 cut(s) 229, 610
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 3 cut(s) 190, 229, 392
NaeI GCCGGC 1 cut(s) 292
NciI CCSGG 2 cut(s) 229, 610
NdeII GATC 3 cut(s) 82, 87, 420
NgoMIV GCCGGC 1 cut(s) 290
NlaIII CATG 3 cut(s) 156, 220, 470
NlaIV GGNNCC 2 cut(s) 145, 305
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 292
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 2 cut(s) 412, 435
PleI GAGTC 3 cut(s) 154, 203, 233
PpsI GAGTC 3 cut(s) 154, 203, 233
Psp124BI GAGCTC 1 cut(s) 108
PspN4I GGNNCC 2 cut(s) 145, 305
PspPI GGNCC 2 cut(s) 248, 612
PstI CTGCAG 1 cut(s) 415
SacI GAGCTC 1 cut(s) 108
SatI GCNGC 2 cut(s) 411, 434
Sau3AI GATC 3 cut(s) 82, 87, 420
Sau96I GGNCC 2 cut(s) 248, 612
SchI GAGTC 3 cut(s) 154, 203, 233
ScrFI CCNGG 2 cut(s) 229, 610
SduI GDGCHC 4 cut(s) 108, 234, 405, 430
SfcI CTRYAG 2 cut(s) 177, 411
Sfr274I CTCGAG 1 cut(s) 162
SinI GGWCC 1 cut(s) 612
SlaI CTCGAG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 162
SmoI CTYRAG 1 cut(s) 162
SphI GCATGC 1 cut(s) 470
SsiI CCGC 4 cut(s) 281, 288, 329, 431
SspMI CTAG 6 cut(s) 333, 453, 548, 572, 638, 646
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 227, 608
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 278
TaqI TCGA 2 cut(s) 163, 234
TfiI GAWTC 1 cut(s) 526
TscAI CASTG 3 cut(s) 186, 266, 443
TseI GCWGC 2 cut(s) 410, 433
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 3 cut(s) 186, 266, 443
VpaK11BI GGWCC 1 cut(s) 612
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 1 cut(s) 470
XhoI CTCGAG 1 cut(s) 162
XspI CTAG 6 cut(s) 333, 453, 548, 572, 638, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.