Rmu_sc0003909.1_g000009

tRNA-specific 2-thiouridylase

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003909.1
N/A
Physical Location & Seq
Forward (+)
53428 .. 54269
842 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 264 bp
atggtttatgactggaggcatgttgtggagtgcaagggtgacaacgcttgtgatgatggacagattgatgacgattcaaggattgttttgaatttcaaggtgcaggtcagacatgggcctcggttttgcaattgtaccttgacaattgaacatggtgaagatggcattgaagatgtagcagttaacttatctgaagatgatcaaggcctggtagcggggcagtttgtggctttctactggggaagaacctgcttacttgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
Pfam Domains
Protein Families

Protein Analysis

87

Amino Acids

9.79

Weight (kDa)

4.41

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_Me_trans_C PF20258 29 - 85 1.2e-09 Aminomethyltransferase beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 94, 257
AciI CCGC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 5 cut(s) 78, 91, 97, 149, 170
AjnI CCWGG 1 cut(s) 207
AoxI GGCC 2 cut(s) 116, 205
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 50, 167
BccI CCATC 2 cut(s) 50, 155
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 199
BfuAI ACCTGC 2 cut(s) 94, 257
Bme1390I CCNGG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 209
BmrI ACTGGG 1 cut(s) 247
BmuI ACTGGG 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 17, 242
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 214
BseNI ACTGG 2 cut(s) 17, 242
BsgI GTGCAG 1 cut(s) 122
BshFI GGCC 2 cut(s) 118, 207
BslI CCNNNNNNNGG 1 cut(s) 214
BsnI GGCC 2 cut(s) 118, 207
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 118, 207
BspMI ACCTGC 2 cut(s) 94, 257
BsrI ACTGG 2 cut(s) 17, 242
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 261
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 209
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 207
BsuRI GGCC 2 cut(s) 118, 207
BveI ACCTGC 2 cut(s) 94, 257
Cfr13I GGNCC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 135
CviAII CATG 3 cut(s) 20, 113, 152
CviJI RGCY 3 cut(s) 118, 207, 230
CviKI_1 RGCY 3 cut(s) 118, 207, 230
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 261
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eco147I AGGCCT 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 207
FaeI CATG 3 cut(s) 23, 116, 155
FaiI YATR 4 cut(s) 9, 21, 114, 153
FatI CATG 3 cut(s) 19, 112, 151
FauI CCCGC 1 cut(s) 208
FbaI TGATCA 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 2 cut(s) 118, 207
Hin1II CATG 3 cut(s) 23, 116, 155
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 1 cut(s) 74
HpaI GTTAAC 1 cut(s) 184
HphI GGTGA 2 cut(s) 50, 167
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 110, 193
Hpy8I GTNNAC 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 33, 103, 129
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 3 cut(s) 23, 116, 155
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 184
Kzo9I GATC 1 cut(s) 199
LpnPI CCDG 4 cut(s) 89, 194, 221, 223
MaeIII GTNAC 1 cut(s) 38
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 4 cut(s) 170, 182, 206, 255
MfeI CAATTG 2 cut(s) 130, 144
MluCI AATT 3 cut(s) 91, 130, 144
MnlI CCTC 2 cut(s) 9, 129
MseI TTAA 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 2 cut(s) 130, 144
MvaI CCWGG 1 cut(s) 209
NdeII GATC 1 cut(s) 199
NlaIII CATG 3 cut(s) 23, 116, 155
NmuCI GTSAC 1 cut(s) 38
NspI RCATGY 1 cut(s) 23
PceI AGGCCT 1 cut(s) 207
PfeI GAWTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspPI GGNCC 1 cut(s) 116
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 183
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 1 cut(s) 116
ScrFI CCNGG 1 cut(s) 209
SetI ASST 4 cut(s) 102, 108, 140, 251
Sse9I AATT 3 cut(s) 91, 130, 144
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 1 cut(s) 215
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 207
TasI AATT 3 cut(s) 91, 130, 144
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 38
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.