Rmu_sc0003953.1_g000023

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003953.1
N/A
Physical Location & Seq
Reverse (-)
91355 .. 92484
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003953.1_g000023.1.cds

Sequence Viewer

Length: 987 bp
atgccatgcatcattctcgagttgcaacacttgaagatccttgatgtccgtgaatgccccagacttgtcagatttccaagcaagtacaaatctcaagttcggtcgaggtcagatgctgacaatatttcacatgacaagggtaacgtgcttcccaagctaaaggcattgagattcacaggatgcaatttatccgatgccgatttccttgggactcttgattgccaagagacattgaccgaacttgatctctcaagtaacaattttgttagtcttcctgcatgcattagcaaattttttaacttggagatacttcagttgcgttgttgcactagacttcgagaaataccagagcttccatcaaatttggtttccgtagatgtgagttattgtgaatcactggagaggtttgaaaacttttcaaaggaaatattaaaaaaagaatcacaacggagtggttcgatcaagatgcggaattgccatcgattgtgtgataatctgggctttgacgacatggcaatcaatttggaaagtatgttgctaaatgagttcaagcttgaagttgaacgaggaaacatgaaggaggtctatgcctcattcttcaacgactactctcctcccaggcctcggtcttctttttcatcttcatcaacatctattccttcaccggtcaagaacctcctttcgttcaccatcatattcttgctaagcatctttagcatgtacctgtttgacatgcagagagaggattataatatagataccatcacgccagaccatggccgcctccactctcatgctgctggaaatgataagagttgcttattcgtataccagcttgtatgtatgcttaatggtttgccgaatgaaggtggaatcacggcggcaggttacatggtgttgtgtctgttgattgatgaggttgacagcaacaaacttgctcgtgtaattgctgcatctcggccccaaatgttggtttattgggcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

328

Amino Acids

37.26

Weight (kDa)

5.93

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 875
AccB7I CCANNNNNTGG 2 cut(s) 776, 970
AccI GTMKAC 1 cut(s) 828
AciI CCGC 3 cut(s) 469, 781, 881
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 778
AcsI RAATTY 2 cut(s) 290, 361
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 2 cut(s) 86, 722
AfiI CCNNNNNNNGG 4 cut(s) 624, 776, 866, 970
AgeI ACCGGT 1 cut(s) 664
AgsI TTSAA 7 cut(s) 34, 410, 420, 550, 557, 563, 601
AjnI CCWGG 1 cut(s) 617
AluBI AGCT 4 cut(s) 157, 352, 553, 835
AluI AGCT 4 cut(s) 157, 352, 553, 835
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 3 cut(s) 620, 778, 959
ApeKI GCWGC 2 cut(s) 797, 950
ApoI RAATTY 2 cut(s) 290, 361
AsiGI ACCGGT 1 cut(s) 664
AspS9I GGNCC 1 cut(s) 960
AsuHPI GGTGA 2 cut(s) 654, 679
AvaI CYCGRG 1 cut(s) 17
BaeI ACNNNNGTAYC 2 cut(s) 299, 332
BauI CACGAG 1 cut(s) 939
BbsI GAAGAC 2 cut(s) 263, 621
BbvI GCAGC 2 cut(s) 784, 937
BccI CCATC 4 cut(s) 364, 486, 698, 770
BceAI ACGGC 1 cut(s) 894
BcgI CGANNNNNNTGC 3 cut(s) 32, 448, 482
BciT130I CCWGG 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 875
BisI GCNGC 4 cut(s) 781, 798, 882, 951
BlpI GCTNAGC 1 cut(s) 704
BlsI GCNGC 4 cut(s) 782, 799, 883, 952
Bme1390I CCNGG 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 17
BmgT120I GGNCC 1 cut(s) 960
BmiI GGNNCC 1 cut(s) 962
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 7 cut(s) 18, 103, 170, 184, 456, 717, 962
BpiI GAAGAC 2 cut(s) 263, 621
BpmI CTGGAG 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 704
BpuEI CTTGAG 2 cut(s) 78, 235
Bsa29I ATCGAT 1 cut(s) 481
BsaJI CCNNGG 4 cut(s) 205, 617, 623, 775
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 4 cut(s) 624, 776, 866, 970
Bse118I RCCGGY 1 cut(s) 664
Bse1I ACTGG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 619
BseCI ATCGAT 1 cut(s) 481
BseDI CCNNGG 4 cut(s) 205, 617, 623, 775
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 4 cut(s) 624, 776, 866, 970
BseNI ACTGG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 2 cut(s) 784, 937
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 3 cut(s) 622, 780, 961
BshTI ACCGGT 1 cut(s) 664
BshVI ATCGAT 1 cut(s) 481
BsiEI CGRYCG 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 1 cut(s) 665
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 4 cut(s) 624, 776, 866, 970
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 223
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 3 cut(s) 622, 780, 961
BsoBI CYCGRG 1 cut(s) 17
Bsp143I GATC 3 cut(s) 36, 244, 459
Bsp1720I GCTNAGC 1 cut(s) 704
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 3 cut(s) 469, 781, 881
BspANI GGCC 3 cut(s) 622, 780, 961
BspDI ATCGAT 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 962
BspMI ACCTGC 1 cut(s) 875
BspPI GGATC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 664
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 4 cut(s) 205, 617, 623, 775
BssMI GATC 3 cut(s) 36, 244, 459
BssNAI GTATAC 1 cut(s) 829
BssSI CACGAG 1 cut(s) 939
BssT1I CCWWGG 2 cut(s) 205, 775
Bst1107I GTATAC 1 cut(s) 829
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 619
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 1 cut(s) 704
BstDSI CCRYGG 1 cut(s) 775
BstF5I GGATG 1 cut(s) 185
BstKTI GATC 3 cut(s) 39, 247, 462
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 3 cut(s) 36, 244, 459
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 154, 714
BstNI CCWGG 1 cut(s) 619
BstNSI RCATGY 3 cut(s) 282, 721, 736
BstSCI CCNGG 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 784, 937
BstV2I GAAGAC 2 cut(s) 263, 621
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BstZ17I GTATAC 1 cut(s) 829
Bsu15I ATCGAT 1 cut(s) 481
BsuRI GGCC 3 cut(s) 622, 780, 961
BsuTUI ATCGAT 1 cut(s) 481
BtgI CCRYGG 1 cut(s) 775
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 395
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 1 cut(s) 280
CaiI CAGNNNCTG 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 960
ClaI ATCGAT 1 cut(s) 481
Csp6I GTAC 2 cut(s) 85, 721
CspAI ACCGGT 1 cut(s) 664
CviJI RGCY 9 cut(s) 157, 352, 501, 553, 622, 780, 835, 961, 983
CviKI_1 RGCY 9 cut(s) 157, 352, 501, 553, 622, 780, 835, 961, 983
CviQI GTAC 2 cut(s) 85, 721
DdeI CTNAG 1 cut(s) 704
DpnI GATC 3 cut(s) 38, 246, 461
DpnII GATC 3 cut(s) 36, 244, 459
EaeI YGGCCR 1 cut(s) 778
Eco130I CCWWGG 2 cut(s) 205, 775
Eco147I AGGCCT 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 617
EcoT14I CCWWGG 2 cut(s) 205, 775
EcoT22I ATGCAT 2 cut(s) 11, 284
ErhI CCWWGG 2 cut(s) 205, 775
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FaqI GGGAC 1 cut(s) 223
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 4 cut(s) 781, 798, 882, 951
FokI GGATG 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 781, 798, 882, 951
FspBI CTAG 1 cut(s) 330
GluI GCNGC 4 cut(s) 781, 798, 882, 951
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 3 cut(s) 622, 780, 961
HapII CCGG 1 cut(s) 665
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 5 cut(s) 171, 211, 392, 440, 873
HpaII CCGG 1 cut(s) 665
HphI GGTGA 2 cut(s) 654, 679
Hpy166II GTNNAC 3 cut(s) 687, 829, 922
Hpy188I TCNGA 3 cut(s) 71, 112, 193
Hpy188III TCNNGA 5 cut(s) 17, 215, 338, 463, 670
Hpy8I GTNNAC 3 cut(s) 687, 829, 922
HpyAV CCTTC 3 cut(s) 571, 669, 860
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 8 cut(s) 9, 25, 183, 278, 282, 327, 736, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 714
HpyF3I CTNAG 1 cut(s) 704
HpySE526I ACGT 1 cut(s) 144
Kzo9I GATC 3 cut(s) 36, 244, 459
Lsp1109I GCAGC 2 cut(s) 784, 937
LweI GCATC 7 cut(s) 18, 103, 170, 184, 456, 717, 962
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 3 cut(s) 140, 254, 887
MalI GATC 3 cut(s) 38, 246, 461
MboI GATC 3 cut(s) 36, 244, 459
MboII GAAGA 5 cut(s) 46, 263, 589, 621, 633
MflI RGATCY 1 cut(s) 36
MluCI AATT 7 cut(s) 184, 259, 290, 361, 472, 520, 945
MlyI GAGTC 1 cut(s) 205
Mph1103I ATGCAT 2 cut(s) 11, 284
MseI TTAA 3 cut(s) 297, 431, 849
MslI CAYNNNNRTG 1 cut(s) 792
MspI CCGG 1 cut(s) 665
MspR9I CCNGG 1 cut(s) 619
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 1 cut(s) 619
MwoI GCNNNNNNNGC 2 cut(s) 154, 714
NcoI CCATGG 1 cut(s) 775
NdeII GATC 3 cut(s) 36, 244, 459
NlaIV GGNNCC 1 cut(s) 962
NmeAIII GCCGAG 1 cut(s) 937
NsiI ATGCAT 2 cut(s) 11, 284
NspI RCATGY 3 cut(s) 282, 721, 736
PaeI GCATGC 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 17
PceI AGGCCT 1 cut(s) 622
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 4 cut(s) 171, 392, 440, 873
PflMI CCANNNNNTGG 2 cut(s) 776, 970
PinAI ACCGGT 1 cut(s) 664
PkrI GCNGC 4 cut(s) 782, 799, 883, 952
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PsiI TTATAA 1 cut(s) 750
Psp6I CCWGG 1 cut(s) 617
PspGI CCWGG 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 962
PspPI GGNCC 1 cut(s) 960
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 2 cut(s) 86, 722
RsaNI GTAC 2 cut(s) 85, 721
RseI CAYNNNNRTG 1 cut(s) 792
SaqAI TTAA 3 cut(s) 297, 431, 849
SatI GCNGC 4 cut(s) 781, 798, 882, 951
Sau3AI GATC 3 cut(s) 36, 244, 459
Sau96I GGNCC 1 cut(s) 960
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 619
SfaNI GCATC 7 cut(s) 18, 103, 170, 184, 456, 717, 962
Sfr274I CTCGAG 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 3 cut(s) 17, 93, 250
SmoI CTYRAG 3 cut(s) 17, 93, 250
SphI GCATGC 1 cut(s) 282
Sse9I AATT 7 cut(s) 184, 259, 290, 361, 472, 520, 945
SseBI AGGCCT 1 cut(s) 622
SsiI CCGC 3 cut(s) 469, 781, 881
SspI AATATT 2 cut(s) 124, 429
SspMI CTAG 1 cut(s) 330
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 2 cut(s) 205, 775
TaiI ACGT 1 cut(s) 147
TaqI TCGA 5 cut(s) 18, 104, 337, 458, 481
TaqII GACCGA 3 cut(s) 90, 251, 615
TasI AATT 7 cut(s) 184, 259, 290, 361, 472, 520, 945
TatI WGTACW 1 cut(s) 84
TauI GCSGC 2 cut(s) 783, 884
TfiI GAWTC 4 cut(s) 171, 392, 440, 873
Tru1I TTAA 3 cut(s) 297, 431, 849
Tru9I TTAA 3 cut(s) 297, 431, 849
TscAI CASTG 1 cut(s) 402
TseI GCWGC 2 cut(s) 797, 950
TspDTI ATGAA 4 cut(s) 590, 627, 633, 879
TspGWI ACGGA 3 cut(s) 38, 361, 463
TspRI CASTG 1 cut(s) 402
Van91I CCANNNNNTGG 2 cut(s) 776, 970
XapI RAATTY 2 cut(s) 290, 361
XceI RCATGY 3 cut(s) 282, 721, 736
XhoI CTCGAG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 2 cut(s) 11, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.