Rmu_sc0003964.1_g000011

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003964.1
N/A
Physical Location & Seq
Reverse (-)
32976 .. 38451
5476 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
atgtcctccccaaccccccgccgccgtgctggaccgacaacgaggccgaagccctcatctccatcttcaaggacaagtggtcctctctcggccgccaccgactgggatgacgttgccaccaccctcgcctcccaattcccactggtctcgccgttcaagacctcctcgcaatgccacaacaagattgagaagcttaggagagaagtggaggtgagtttgaaggtcgaacaagaacatctttgggacgatatcggagaagaagacaatggctgccagcctgccactgataaaatctccaagctcaaatctgaggtcaccgctctcaaccagatcagagcgggttttgcccgcttcttagcagacgacgatccaatggagaacttcactaccaccaacctcaacgacgctggtcgtagaaatctttattttaggtttattgctggtaaacctagttatcaatccgcccgcgtcaatgaccctgagtttgctattcccattgttgatatgtcacttctcacctctggttctcctgatcagcgcgcccaaatcgtcctcgatcttgtcaaaatttgtgaagaatggggcttcttcatcgcaattaaccacggagtaccagaaagcctaatgaaggggatgatcgacgcatgccatggatttttcagtcttccagatgaggagaaggagtttaaatcaggaaatgatgttcttgagatgttcaagtatggtacaagttataatcttgcattggataaagttcttctatggagggacttctttaaggccagaacacatccagagttctactccctccacaaaccagctagcttcagtgaggtttcactggagttcggcaaaagaacaagagaagtggcactagaaatcacaaaagcaatatcagaaagcctgggattggagccgaattacatatacgagacaatgaacatggattgcggcttacaattgctagccggcaattactacccaccttgccctcagccagaacatgcaatcggtataccacatcataccgatcacggtctagtcacactcctcatccagaatgagatgaatggcctccaagtcgaacacaacggaaaatggctcactgttaatggcccttccaatggcttctttgtgaaccttgctgatcaaatgcagattctaaccaacggtaaatacaagagtgtgatgcatagggcgactgtgaataacacagcgacaaggatatccatagccataccacatggaccttctatagatacaatcatcgcaccagcaccagagttgtgtgaaagagaaggccaagctccaaagtaccttgcaatgaactataaagagtacatacaacttcaacagagtggcaagaactacacactactagcaaaacaacaattacccacggattttaatctgtgggtaataagacttttacacacggatatcagtgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

483

Amino Acids

54.17

Weight (kDa)

5.82

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 164 - 266 2.4e-22 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 320 - 414 1.4e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
AccB7I CCANNNNNTGG 1 cut(s) 102
AccBSI CCGCTC 2 cut(s) 320, 338
AccI GTMKAC 1 cut(s) 1015
AccII CGCG 2 cut(s) 468, 540
AciI CCGC 9 cut(s) 19, 22, 93, 318, 338, 349, 462, 466, 951
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 567
AcuI CTGAAG 1 cut(s) 811
AfaI GTAC 4 cut(s) 612, 727, 1316, 1340
AfiI CCNNNNNNNGG 4 cut(s) 102, 628, 910, 1124
AgsI TTSAA 5 cut(s) 69, 157, 220, 718, 1352
AhdI GACNNNNNGTC 1 cut(s) 78
AjnI CCWGG 1 cut(s) 903
AloI GAACNNNNNNTCC 2 cut(s) 687, 719
AluBI AGCT 5 cut(s) 193, 301, 821, 825, 1307
AluI AGCT 5 cut(s) 193, 301, 821, 825, 1307
Alw26I GTCTC 2 cut(s) 151, 926
AlwI GGATC 1 cut(s) 362
AoxI GGCC 6 cut(s) 44, 90, 780, 1072, 1114, 1300
ApeKI GCWGC 1 cut(s) 270
ApoI RAATTY 1 cut(s) 567
ArsI GACNNNNNNTTYG 4 cut(s) 297, 329, 467, 499
AspLEI GCGC 2 cut(s) 540, 542
AspS9I GGNCC 4 cut(s) 32, 80, 1115, 1247
AsuHPI GGTGA 3 cut(s) 223, 307, 508
AsuNHI GCTAGC 2 cut(s) 821, 964
AvaII GGWCC 3 cut(s) 32, 80, 1247
BbsI GAAGAC 2 cut(s) 267, 656
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 1 cut(s) 257
BccI CCATC 1 cut(s) 70
BceAI ACGGC 2 cut(s) 9, 136
BciT130I CCWGG 1 cut(s) 905
BclI TGATCA 2 cut(s) 532, 1147
BcoDI GTCTC 2 cut(s) 151, 926
BfaI CTAG 6 cut(s) 450, 822, 875, 965, 1040, 1379
BfmI CTRYAG 1 cut(s) 1254
BisI GCNGC 4 cut(s) 22, 93, 271, 952
BlsI GCNGC 4 cut(s) 23, 94, 272, 953
Bme1390I CCNGG 1 cut(s) 905
Bme18I GGWCC 3 cut(s) 32, 80, 1247
BmeRI GACNNNNNGTC 1 cut(s) 78
BmgT120I GGNCC 4 cut(s) 32, 80, 1115, 1247
BmiI GGNNCC 1 cut(s) 915
BmrFI CCNGG 1 cut(s) 905
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 1179
BmtI GCTAGC 2 cut(s) 825, 968
BmuI ACTGGG 1 cut(s) 112
BoxI GACNNNNGTC 1 cut(s) 408
BpiI GAAGAC 2 cut(s) 267, 656
BplI GAGNNNNNCTC 2 cut(s) 788, 820
BpmI CTGGAG 1 cut(s) 863
Bpu10I CCTNAGC 2 cut(s) 194, 993
BpuEI CTTGAG 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 1407
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 4 cut(s) 604, 649, 904, 1398
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 628, 910, 1124
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 3 cut(s) 107, 147, 846
Bse3DI GCAATG 2 cut(s) 176, 1329
Bse8I GATNNNNATC 1 cut(s) 1407
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 4 cut(s) 604, 649, 904, 1398
BseGI GGATG 4 cut(s) 112, 639, 790, 1053
BseJI GATNNNNATC 1 cut(s) 1407
BseLI CCNNNNNNNGG 4 cut(s) 102, 628, 910, 1124
BseMI GCAATG 2 cut(s) 176, 1329
BseMII CTCAG 3 cut(s) 300, 471, 1007
BseNI ACTGG 3 cut(s) 107, 147, 846
BsePI GCGCGC 1 cut(s) 538
BseRI GAGGAG 3 cut(s) 154, 689, 1040
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 1 cut(s) 257
Bsh1236I CGCG 2 cut(s) 468, 540
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 6 cut(s) 46, 92, 782, 1074, 1116, 1302
BsiEI CGRYCG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 969
BslFI GGGAC 2 cut(s) 257, 782
BslI CCNNNNNNNGG 4 cut(s) 102, 628, 910, 1124
BsmAI GTCTC 2 cut(s) 151, 926
BsmFI GGGAC 2 cut(s) 257, 782
BsnI GGCC 6 cut(s) 46, 92, 782, 1074, 1116, 1302
Bso31I GGTCTC 1 cut(s) 151
Bsp143I GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 9 cut(s) 19, 22, 93, 318, 338, 349, 462, 466, 951
BspANI GGCC 6 cut(s) 46, 92, 782, 1074, 1116, 1302
BspCNI CTCAG 3 cut(s) 301, 472, 1006
BspFNI CGCG 2 cut(s) 468, 540
BspLI GGNNCC 1 cut(s) 915
BspOI GCTAGC 2 cut(s) 825, 968
BspPI GGATC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 2 cut(s) 320, 338
BsrDI GCAATG 2 cut(s) 176, 1329
BsrFI RCCGGY 1 cut(s) 968
BsrI ACTGG 3 cut(s) 107, 147, 846
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 4 cut(s) 604, 649, 904, 1398
BssHII GCGCGC 1 cut(s) 538
BssMI GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
BssNAI GTATAC 1 cut(s) 1016
BssT1I CCWWGG 1 cut(s) 649
Bst1107I GTATAC 1 cut(s) 1016
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 4 cut(s) 1037, 1108, 1172, 1204
BstC8I GCNNGC 9 cut(s) 275, 279, 349, 466, 540, 646, 823, 966, 970
BstDEI CTNAG 5 cut(s) 194, 309, 355, 480, 993
BstDSI CCRYGG 3 cut(s) 604, 649, 1398
BstEII GGTNACC 1 cut(s) 313
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 4 cut(s) 112, 639, 790, 1053
BstFNI CGCG 2 cut(s) 468, 540
BstHHI GCGC 2 cut(s) 540, 542
BstKTI GATC 7 cut(s) 333, 370, 535, 559, 639, 1033, 1150
BstMAI GTCTC 2 cut(s) 151, 926
BstMBI GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 905
BstNSI RCATGY 2 cut(s) 648, 1007
BstPAI GACNNNNGTC 1 cut(s) 408
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 1 cut(s) 903
BstSFI CTRYAG 1 cut(s) 1254
BstUI CGCG 2 cut(s) 468, 540
BstV1I GCAGC 1 cut(s) 257
BstV2I GAAGAC 2 cut(s) 267, 656
BstZ17I GTATAC 1 cut(s) 1016
BstZI CGGCCG 1 cut(s) 90
BsuRI GGCC 6 cut(s) 46, 92, 782, 1074, 1116, 1302
BtgI CCRYGG 3 cut(s) 604, 649, 1398
BtgZI GCGATG 2 cut(s) 577, 1252
BtsCI GGATG 4 cut(s) 112, 639, 790, 1053
BtsIMutI CAGTG 6 cut(s) 140, 282, 835, 839, 1104, 1450
Cac8I GCNNGC 9 cut(s) 275, 279, 349, 466, 540, 646, 823, 966, 970
CfoI GCGC 2 cut(s) 540, 542
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 4 cut(s) 32, 80, 1115, 1247
CseI GACGC 3 cut(s) 413, 457, 650
Csp6I GTAC 4 cut(s) 611, 726, 1315, 1339
CspCI CAANNNNNGTGG 2 cut(s) 849, 884
CviAII CATG 5 cut(s) 645, 650, 943, 1004, 1244
CviQI GTAC 4 cut(s) 611, 726, 1315, 1339
DdeI CTNAG 5 cut(s) 194, 309, 355, 480, 993
DpnI GATC 7 cut(s) 332, 369, 534, 558, 638, 1032, 1149
DpnII GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
DraI TTTAAA 1 cut(s) 688
DriI GACNNNNNGTC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 90
EagI CGGCCG 1 cut(s) 90
Eam1105I GACNNNNNGTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 451
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 151
Eco32I GATATC 3 cut(s) 250, 1227, 1441
Eco47I GGWCC 3 cut(s) 32, 80, 1247
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 811
Eco91I GGTNACC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 903
EcoRV GATATC 3 cut(s) 250, 1227, 1441
EcoT14I CCWWGG 1 cut(s) 649
EcoT22I ATGCAT 1 cut(s) 1194
ErhI CCWWGG 1 cut(s) 649
FaeI CATG 5 cut(s) 648, 653, 946, 1007, 1247
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 2 cut(s) 257, 782
FatI CATG 5 cut(s) 644, 649, 942, 1003, 1243
FauI CCCGC 4 cut(s) 26, 331, 356, 473
FbaI TGATCA 2 cut(s) 532, 1147
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 4 cut(s) 22, 93, 271, 952
FokI GGATG 4 cut(s) 119, 646, 777, 1040
Fsp4HI GCNGC 4 cut(s) 22, 93, 271, 952
FspBI CTAG 6 cut(s) 450, 822, 875, 965, 1040, 1379
GlaI GCGC 2 cut(s) 539, 541
GluI GCNGC 4 cut(s) 22, 93, 271, 952
GsuI CTGGAG 1 cut(s) 863
HaeIII GGCC 6 cut(s) 46, 92, 782, 1074, 1116, 1302
HapII CCGG 1 cut(s) 969
HgaI GACGC 3 cut(s) 413, 457, 650
HhaI GCGC 2 cut(s) 540, 542
Hin1II CATG 5 cut(s) 648, 653, 946, 1007, 1247
Hin6I GCGC 2 cut(s) 538, 540
HinP1I GCGC 2 cut(s) 538, 540
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 1 cut(s) 1159
HpaII CCGG 1 cut(s) 969
HphI GGTGA 3 cut(s) 223, 307, 508
Hpy166II GTNNAC 3 cut(s) 446, 1016, 1138
Hpy188I TCNGA 4 cut(s) 254, 310, 335, 898
Hpy188III TCNNGA 7 cut(s) 157, 530, 668, 693, 707, 794, 1057
Hpy8I GTNNAC 3 cut(s) 446, 1016, 1138
Hpy99I CGWCG 3 cut(s) 368, 407, 644
HpyAV CCTTC 6 cut(s) 214, 622, 673, 1128, 1260, 1292
HpyCH4III ACNGT 4 cut(s) 1037, 1108, 1172, 1204
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 5 cut(s) 743, 1007, 1156, 1192, 1322
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 5 cut(s) 194, 309, 355, 480, 993
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 5 cut(s) 648, 653, 946, 1007, 1247
HspAI GCGC 2 cut(s) 538, 540
KroI GCCGGC 1 cut(s) 968
KroNI GCCGGC 1 cut(s) 970
Ksp22I TGATCA 2 cut(s) 532, 1147
Kzo9I GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
LmnI GCTCC 2 cut(s) 913, 1312
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 1179
MaeI CTAG 6 cut(s) 450, 822, 875, 965, 1040, 1379
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 313, 507, 1042
MalI GATC 7 cut(s) 332, 369, 534, 558, 638, 1032, 1149
MbiI CCGCTC 2 cut(s) 320, 338
MboI GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
MboII GAAGA 7 cut(s) 57, 269, 272, 580, 587, 656, 749
MfeI CAATTG 1 cut(s) 959
MluCI AATT 7 cut(s) 134, 567, 597, 919, 959, 973, 1391
Mph1103I ATGCAT 1 cut(s) 1194
MroNI GCCGGC 1 cut(s) 968
MseI TTAA 5 cut(s) 600, 687, 777, 1110, 1407
MspI CCGG 1 cut(s) 969
MspR9I CCNGG 1 cut(s) 905
MunI CAATTG 1 cut(s) 959
MvaI CCWGG 1 cut(s) 905
MvnI CGCG 2 cut(s) 468, 540
MwoI GCNNNNNNNGC 1 cut(s) 344
NaeI GCCGGC 1 cut(s) 970
NcoI CCATGG 1 cut(s) 649
NdeII GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
NgoMIV GCCGGC 1 cut(s) 968
NheI GCTAGC 2 cut(s) 821, 964
NlaIII CATG 5 cut(s) 648, 653, 946, 1007, 1247
NlaIV GGNNCC 1 cut(s) 915
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 3 cut(s) 313, 507, 1042
NsiI ATGCAT 1 cut(s) 1194
NspI RCATGY 2 cut(s) 648, 1007
PaeI GCATGC 1 cut(s) 648
PauI GCGCGC 1 cut(s) 538
PdiI GCCGGC 1 cut(s) 970
PfeI GAWTC 1 cut(s) 1159
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 4 cut(s) 23, 94, 272, 953
PshAI GACNNNNGTC 1 cut(s) 408
PsiI TTATAA 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 903
PspEI GGTNACC 1 cut(s) 313
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 1 cut(s) 915
PspPI GGNCC 4 cut(s) 32, 80, 1115, 1247
PteI GCGCGC 1 cut(s) 538
RsaI GTAC 4 cut(s) 612, 727, 1316, 1340
RsaNI GTAC 4 cut(s) 611, 726, 1315, 1339
SaqAI TTAA 5 cut(s) 600, 687, 777, 1110, 1407
SatI GCNGC 4 cut(s) 22, 93, 271, 952
Sau3AI GATC 7 cut(s) 330, 367, 532, 556, 636, 1030, 1147
Sau96I GGNCC 4 cut(s) 32, 80, 1115, 1247
ScrFI CCNGG 1 cut(s) 905
SfaNI GCATC 1 cut(s) 1179
SfcI CTRYAG 1 cut(s) 1254
SinI GGWCC 3 cut(s) 32, 80, 1247
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
SphI GCATGC 1 cut(s) 648
Sse9I AATT 7 cut(s) 134, 567, 597, 919, 959, 973, 1391
SsiI CCGC 9 cut(s) 19, 22, 93, 318, 338, 349, 462, 466, 951
SspMI CTAG 6 cut(s) 450, 822, 875, 965, 1040, 1379
StyD4I CCNGG 1 cut(s) 903
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 4 cut(s) 1037, 1108, 1172, 1204
TaiI ACGT 1 cut(s) 114
TaqI TCGA 4 cut(s) 225, 555, 639, 1083
TaqII GACCGA 1 cut(s) 49
TasI AATT 7 cut(s) 134, 567, 597, 919, 959, 973, 1391
TatI WGTACW 1 cut(s) 1338
TauI GCSGC 3 cut(s) 24, 95, 954
TfiI GAWTC 1 cut(s) 1159
Tru1I TTAA 5 cut(s) 600, 687, 777, 1110, 1407
Tru9I TTAA 5 cut(s) 600, 687, 777, 1110, 1407
TscAI CASTG 6 cut(s) 147, 289, 835, 846, 1111, 1450
TseFI GTSAC 3 cut(s) 313, 507, 1042
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 3 cut(s) 313, 507, 1042
TspDTI ATGAA 5 cut(s) 580, 641, 953, 1082, 1340
TspGWI ACGGA 4 cut(s) 621, 1107, 1415, 1451
TspRI CASTG 6 cut(s) 147, 289, 835, 846, 1111, 1450
Van91I CCANNNNNTGG 1 cut(s) 102
VpaK11BI GGWCC 3 cut(s) 32, 80, 1247
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 1 cut(s) 567
XceI RCATGY 2 cut(s) 648, 1007
XmiI GTMKAC 1 cut(s) 1015
XspI CTAG 6 cut(s) 450, 822, 875, 965, 1040, 1379
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.