Rmu_sc0004068.1_g000001
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004068.1
N/A
Physical Location & Seq
Forward (+)
6733 .. 9158
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
atggttgttggcattgaaatgaatgcttctgcagttttagatgctcagaggaatgctgaaattaatggcataaagaactgtagatttgtgtgttcaaaggcagagaatgtgatgcggtctttattggaagagtaccctactgcacctgagaaacaagacagtgatgccattcaaggtagtgacaaagcaatagttactgatggcgagaaaatagttgccactgatgaagaaaaagcagtagtaaaggatgaaaaacccaaccctgaagagagtttaagccatgaggctgaaaatggtaaaagtgcatgtgaaggtttagataatagcaagcaggaccttcagaaaagttgcacttctcaaaataggaaaagtccagttcagtgtttcaagaatgtggtagctattgttgatcctccacgcaatggacttcatcccacagtgatcaaagctctgagaactcatcctggactacggaggcttgtctacatctcctgcaatcctgaaagcctagtggcaaatgccatcgagctttgcacaccttcccctgataaaattgaaaagaacaagaacaaccgagcatggagaaacatgagcagtgctggtctggcacggcaaagggcaaagtctatgcccgcttcagagccttttcaacctgtaaaagcaatggcggtcgacatttttccacatactgtacactgcgaattgaaaggggctttagagcaagtgcagcggttgcactttgcccgggcaaaagccatgaaaaccgacgtggtgaccgggcaagagcaaaatcggcgacagcaacagcaggcggtccaggctaacgcgctgaaggcagcgactgggtctccaacggacgacgcgtggcgctgcaaatcccaggcgagtgactacactctcttgtcgtgtagtggatctgctttaggccagcagatgccacgtgctgcagacccgttgggtaattttgaaattgctgcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

326

Amino Acids

35.54

Weight (kDa)

7.99

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 129 - 177 2.9e-07 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 2 cut(s) 483, 672
AccII CGCG 2 cut(s) 827, 863
AciI CCGC 5 cut(s) 115, 633, 668, 730, 812
AclWI GGATC 2 cut(s) 404, 922
AcuI CTGAAG 4 cut(s) 285, 323, 621, 851
AcvI CACGTG 1 cut(s) 941
AfaI GTAC 2 cut(s) 134, 693
AfiI CCNNNNNNNGG 1 cut(s) 422
AflIII ACRYGT 1 cut(s) 861
AgsI TTSAA 8 cut(s) 17, 96, 173, 388, 557, 650, 706, 968
AjiI CACGTC 1 cut(s) 769
AjnI CCWGG 3 cut(s) 463, 816, 879
AluBI AGCT 3 cut(s) 401, 449, 529
AluI AGCT 3 cut(s) 401, 449, 529
Alw26I GTCTC 1 cut(s) 852
AlwI GGATC 2 cut(s) 404, 922
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 744
AoxI GGCC 1 cut(s) 925
ApeKI GCWGC 5 cut(s) 727, 836, 870, 944, 974
AseI ATTAAT 1 cut(s) 63
AspLEI GCGC 2 cut(s) 829, 870
AspS9I GGNCC 2 cut(s) 334, 814
AsuC2I CCSGG 3 cut(s) 745, 746, 778
AsuHPI GGTGA 1 cut(s) 784
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 2 cut(s) 334, 814
BbrPI CACGTG 1 cut(s) 941
BbvI GCAGC 5 cut(s) 739, 848, 857, 931, 961
BccI CCATC 2 cut(s) 194, 530
BceAI ACGGC 1 cut(s) 626
BciT130I CCWGG 3 cut(s) 465, 818, 881
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 3 cut(s) 745, 746, 778
BcoDI GTCTC 1 cut(s) 852
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 3 cut(s) 30, 79, 945
BfoI RGCGCY 1 cut(s) 871
BisI GCNGC 5 cut(s) 728, 837, 871, 945, 975
BlsI GCNGC 5 cut(s) 729, 838, 872, 946, 976
Bme1390I CCNGG 6 cut(s) 465, 745, 746, 778, 818, 881
Bme18I GGWCC 2 cut(s) 334, 814
BmeT110I CYCGRG 1 cut(s) 744
BmgBI CACGTC 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 334, 814
BmrFI CCNGG 6 cut(s) 465, 745, 746, 778, 818, 881
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 31, 102, 154, 924
BmuI ACTGGG 1 cut(s) 852
BpuMI CCSGG 3 cut(s) 745, 746, 778
BsaAI YACGTR 1 cut(s) 941
BsaI GGTCTC 1 cut(s) 852
BsaJI CCNNGG 2 cut(s) 744, 879
BsaXI ACNNNNNCTCC 2 cut(s) 832, 862
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 374, 847
Bse3DI GCAATG 2 cut(s) 427, 669
BseBI CCWGG 3 cut(s) 465, 818, 881
BseDI CCNNGG 2 cut(s) 744, 879
BseGI GGATG 3 cut(s) 253, 430, 460
BseLI CCNNNNNNNGG 1 cut(s) 422
BseMI GCAATG 2 cut(s) 427, 669
BseMII CTCAG 3 cut(s) 59, 138, 443
BseNI ACTGG 2 cut(s) 374, 847
BseXI GCAGC 5 cut(s) 739, 848, 857, 931, 961
BsgI GTGCAG 2 cut(s) 126, 746
Bsh1236I CGCG 2 cut(s) 827, 863
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 1 cut(s) 927
BsiEI CGRYCG 1 cut(s) 672
BsiHKCI CYCGRG 1 cut(s) 744
BsiSI CCGG 2 cut(s) 745, 777
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 852
BsmI GAATGC 2 cut(s) 28, 58
BsnI GGCC 1 cut(s) 927
Bso31I GGTCTC 1 cut(s) 852
BsoBI CYCGRG 1 cut(s) 744
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 409, 441, 914
BspACI CCGC 5 cut(s) 115, 633, 668, 730, 812
BspANI GGCC 1 cut(s) 927
BspCNI CTCAG 3 cut(s) 58, 139, 444
BspFNI CGCG 2 cut(s) 827, 863
BspMAI CTGCAG 2 cut(s) 34, 949
BspPI GGATC 2 cut(s) 404, 922
BspTNI GGTCTC 1 cut(s) 852
BsrDI GCAATG 2 cut(s) 427, 669
BsrGI TGTACA 1 cut(s) 691
BsrI ACTGG 2 cut(s) 374, 847
BssECI CCNNGG 2 cut(s) 744, 879
BssMI GATC 3 cut(s) 409, 441, 914
Bst2UI CCWGG 3 cut(s) 465, 818, 881
Bst4CI ACNGT 4 cut(s) 80, 161, 439, 691
Bst6I CTCTTC 2 cut(s) 123, 261
BstAPI GCANNNNNTGC 1 cut(s) 733
BstAUI TGTACA 1 cut(s) 691
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 4 cut(s) 329, 633, 810, 929
BstDEI CTNAG 3 cut(s) 45, 147, 452
BstEII GGTNACC 1 cut(s) 772
BstF5I GGATG 3 cut(s) 253, 430, 460
BstFNI CGCG 2 cut(s) 827, 863
BstH2I RGCGCY 1 cut(s) 871
BstHHI GCGC 2 cut(s) 829, 870
BstKTI GATC 3 cut(s) 412, 444, 917
BstMAI GTCTC 1 cut(s) 852
BstMBI GATC 3 cut(s) 409, 441, 914
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 6 cut(s) 605, 727, 733, 793, 818, 833
BstNI CCWGG 3 cut(s) 465, 818, 881
BstNSI RCATGY 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 772
BstSCI CCNGG 6 cut(s) 463, 743, 744, 776, 816, 879
BstSFI CTRYAG 3 cut(s) 30, 79, 945
BstUI CGCG 2 cut(s) 827, 863
BstV1I GCAGC 5 cut(s) 739, 848, 857, 931, 961
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
BsuRI GGCC 1 cut(s) 927
BtrI CACGTC 1 cut(s) 769
BtsCI GGATG 3 cut(s) 253, 430, 460
BtsI GCAGTG 2 cut(s) 601, 694
BtsIMutI CAGTG 6 cut(s) 166, 219, 386, 444, 601, 694
Cac8I GCNNGC 4 cut(s) 329, 633, 810, 929
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 829, 870
Cfr13I GGNCC 2 cut(s) 334, 814
Cfr9I CCCGGG 1 cut(s) 744
CseI GACGC 1 cut(s) 869
Csp6I GTAC 2 cut(s) 133, 692
CviAII CATG 5 cut(s) 281, 306, 579, 589, 757
CviQI GTAC 2 cut(s) 133, 692
DdeI CTNAG 3 cut(s) 45, 147, 452
DpnI GATC 3 cut(s) 411, 443, 916
DpnII GATC 3 cut(s) 409, 441, 914
Eam1104I CTCTTC 2 cut(s) 123, 261
EarI CTCTTC 2 cut(s) 123, 261
Eco31I GGTCTC 1 cut(s) 852
Eco47I GGWCC 2 cut(s) 334, 814
Eco57I CTGAAG 4 cut(s) 285, 323, 621, 851
Eco72I CACGTG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 744
Eco91I GGTNACC 1 cut(s) 772
EcoO109I RGGNCCY 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 772
EcoRII CCWGG 3 cut(s) 463, 816, 879
FaeI CATG 5 cut(s) 284, 309, 582, 592, 760
FaiI YATR 8 cut(s) 71, 282, 307, 580, 590, 629, 687, 758
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FatI CATG 5 cut(s) 280, 305, 578, 588, 756
FauI CCCGC 1 cut(s) 640
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 2 cut(s) 483, 672
Fnu4HI GCNGC 5 cut(s) 728, 837, 871, 945, 975
FokI GGATG 3 cut(s) 260, 417, 447
Fsp4HI GCNGC 5 cut(s) 728, 837, 871, 945, 975
FspBI CTAG 1 cut(s) 509
GlaI GCGC 2 cut(s) 828, 869
GluI GCNGC 5 cut(s) 728, 837, 871, 945, 975
HaeII RGCGCY 1 cut(s) 871
HaeIII GGCC 1 cut(s) 927
HapII CCGG 2 cut(s) 745, 777
HgaI GACGC 1 cut(s) 869
HhaI GCGC 2 cut(s) 829, 870
Hin1II CATG 5 cut(s) 284, 309, 582, 592, 760
Hin6I GCGC 2 cut(s) 827, 868
HinP1I GCGC 2 cut(s) 827, 868
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HpaII CCGG 2 cut(s) 745, 777
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 3 cut(s) 484, 673, 694
Hpy188I TCNGA 4 cut(s) 48, 342, 453, 640
Hpy188III TCNNGA 2 cut(s) 388, 500
Hpy8I GTNNAC 3 cut(s) 484, 673, 694
Hpy99I CGWCG 2 cut(s) 770, 863
HpyAV CCTTC 4 cut(s) 305, 347, 549, 826
HpyCH4III ACNGT 4 cut(s) 80, 161, 439, 691
HpyCH4IV ACGT 2 cut(s) 768, 940
HpyF10VI GCNNNNNNNGC 6 cut(s) 605, 727, 733, 793, 818, 833
HpyF3I CTNAG 3 cut(s) 45, 147, 452
HpySE526I ACGT 2 cut(s) 768, 940
Hsp92II CATG 5 cut(s) 284, 309, 582, 592, 760
HspAI GCGC 2 cut(s) 827, 868
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 3 cut(s) 409, 441, 914
Lsp1109I GCAGC 5 cut(s) 739, 848, 857, 931, 961
LweI GCATC 4 cut(s) 31, 102, 154, 924
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 768, 940
MaeIII GTNAC 4 cut(s) 179, 193, 772, 887
MalI GATC 3 cut(s) 411, 443, 916
MboI GATC 3 cut(s) 409, 441, 914
MboII GAAGA 3 cut(s) 140, 239, 278
MflI RGATCY 1 cut(s) 914
MluCI AATT 5 cut(s) 60, 552, 701, 961, 969
MluI ACGCGT 1 cut(s) 861
MmeI TCCRAC 1 cut(s) 875
MnlI CCTC 4 cut(s) 42, 277, 423, 468
MseI TTAA 2 cut(s) 63, 275
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 730
MspI CCGG 2 cut(s) 745, 777
MspR9I CCNGG 6 cut(s) 465, 745, 746, 778, 818, 881
Mva1269I GAATGC 2 cut(s) 28, 58
MvaI CCWGG 3 cut(s) 465, 818, 881
MvnI CGCG 2 cut(s) 827, 863
MwoI GCNNNNNNNGC 6 cut(s) 605, 727, 733, 793, 818, 833
NciI CCSGG 3 cut(s) 745, 746, 778
NdeII GATC 3 cut(s) 409, 441, 914
NlaIII CATG 5 cut(s) 284, 309, 582, 592, 760
NmuCI GTSAC 3 cut(s) 179, 772, 887
NspI RCATGY 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 860
PctI GAATGC 2 cut(s) 28, 58
PflFI GACNNNGTC 1 cut(s) 844
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 463
PkrI GCNGC 5 cut(s) 729, 838, 872, 946, 976
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 3 cut(s) 463, 816, 879
PspCI CACGTG 1 cut(s) 941
PspEI GGTNACC 1 cut(s) 772
PspGI CCWGG 3 cut(s) 463, 816, 879
PspPI GGNCC 2 cut(s) 334, 814
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 2 cut(s) 34, 949
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 914
PsyI GACNNNGTC 1 cut(s) 844
RsaI GTAC 2 cut(s) 134, 693
RsaNI GTAC 2 cut(s) 133, 692
RseI CAYNNNNRTG 1 cut(s) 17
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 2 cut(s) 63, 275
SatI GCNGC 5 cut(s) 728, 837, 871, 945, 975
Sau3AI GATC 3 cut(s) 409, 441, 914
Sau96I GGNCC 2 cut(s) 334, 814
ScrFI CCNGG 6 cut(s) 465, 745, 746, 778, 818, 881
SfaNI GCATC 4 cut(s) 31, 102, 154, 924
SfcI CTRYAG 3 cut(s) 30, 79, 945
SinI GGWCC 2 cut(s) 334, 814
SmaI CCCGGG 1 cut(s) 746
SmiMI CAYNNNNRTG 1 cut(s) 17
SrfI GCCCGGGC 1 cut(s) 746
Sse9I AATT 5 cut(s) 60, 552, 701, 961, 969
SsiI CCGC 5 cut(s) 115, 633, 668, 730, 812
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 6 cut(s) 463, 743, 744, 776, 816, 879
TaaI ACNGT 4 cut(s) 80, 161, 439, 691
TaiI ACGT 2 cut(s) 771, 943
TaqI TCGA 2 cut(s) 525, 672
TasI AATT 5 cut(s) 60, 552, 701, 961, 969
TatI WGTACW 1 cut(s) 691
Tru1I TTAA 2 cut(s) 63, 275
Tru9I TTAA 2 cut(s) 63, 275
TscAI CASTG 6 cut(s) 166, 226, 386, 444, 601, 701
TseFI GTSAC 3 cut(s) 179, 772, 887
TseI GCWGC 5 cut(s) 727, 836, 870, 944, 974
Tsp45I GTSAC 3 cut(s) 179, 772, 887
TspDTI ATGAA 5 cut(s) 35, 240, 264, 419, 773
TspGWI ACGGA 2 cut(s) 487, 869
TspMI CCCGGG 1 cut(s) 744
TspRI CASTG 6 cut(s) 166, 226, 386, 444, 601, 701
Tth111I GACNNNGTC 1 cut(s) 844
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 334, 814
VspI ATTAAT 1 cut(s) 63
XceI RCATGY 1 cut(s) 309
XmaI CCCGGG 1 cut(s) 744
XmiI GTMKAC 2 cut(s) 483, 672
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.