Rmu_sc0004082.1_g000029

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004082.1
N/A
Physical Location & Seq
Reverse (-)
122987 .. 126330
3344 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
atggtgggccacagaccacgcttgtcgctgtttaacatgccgtatgttaatgagcctcataattatggcttacaagtaggagattatgaaagtctcctcaacctgaccttagcatcagatagagttgggcttcaggttgctattcatgctataggcgatcgagcaaatgacttgatcctcgacatgtatgagtcagtcatttcaaaaaacggagtgagggatcgaagattcaggattgagcatgcgcagcatctggcacctgggacaccagccagattcggtaaactagggattgttgcttcagtacaggttgatcacactactccttgggctcagagatcttatttattcaagtcactcttgggtagcaatgtagaattggcatttggctccgactggccagttgtagacattaatcctttaggtggtattaagacggcaatgaaaaggatgcccccggcttgggatattgcttgggttccatcagagagcgtttctctgaatgactcactgaaagcgtatactctttcagctgctcgtgcatgctttcttgacagtgatctgggatctttgtctcctggaaaactggcagattttgtcgtactttccatatattcatgggatgactttgtggcaggaggatctgcatccattgacgcaacttatgttggtggtgtacaagcctatccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidohydro_3

Protein Analysis

230

Amino Acids

25.07

Weight (kDa)

5.82

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_3 PF07969 24 - 228 1.9e-32 Amidohydrolase family
Amidohydro_1 PF01979 38 - 225 1.5e-10 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 246
AccB1I GGYRCC 1 cut(s) 256
AccI GTMKAC 2 cut(s) 408, 521
AclWI GGATC 4 cut(s) 169, 228, 574, 649
AcoI YGGCCR 1 cut(s) 398
AcuI CTGAAG 2 cut(s) 116, 285
AfaI GTAC 3 cut(s) 306, 603, 678
AfiI CCNNNNNNNGG 3 cut(s) 425, 462, 463
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 204, 352
AjnI CCWGG 2 cut(s) 259, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AluBI AGCT 1 cut(s) 533
AluI AGCT 1 cut(s) 533
Alw26I GTCTC 2 cut(s) 98, 579
AlwI GGATC 4 cut(s) 169, 228, 574, 649
AlwNI CAGNNNCTG 1 cut(s) 253
AoxI GGCC 2 cut(s) 7, 398
ApeKI GCWGC 2 cut(s) 247, 533
AseI ATTAAT 1 cut(s) 414
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 458
BalI TGGCCA 1 cut(s) 400
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 334
BauI CACGAG 1 cut(s) 537
BbvI GCAGC 2 cut(s) 259, 520
BccI CCATC 1 cut(s) 490
BceAI ACGGC 2 cut(s) 25, 453
BciT130I CCWGG 2 cut(s) 261, 579
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 98, 579
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 150
BglII AGATCT 1 cut(s) 338
BisI GCNGC 2 cut(s) 248, 534
BlsI GCNGC 2 cut(s) 249, 535
Bme1390I CCNGG 3 cut(s) 261, 458, 579
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 3 cut(s) 258, 391, 480
BmrFI CCNGG 3 cut(s) 261, 458, 579
BmsI GCATC 4 cut(s) 122, 259, 441, 656
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu10I CCTNAGC 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 646
BsaJI CCNNGG 3 cut(s) 260, 326, 456
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 462, 463
Bse1I ACTGG 3 cut(s) 401, 401, 591
Bse3DI GCAATG 2 cut(s) 376, 447
Bse8I GATNNNNATC 1 cut(s) 646
BseBI CCWGG 2 cut(s) 261, 579
BseDI CCNNGG 3 cut(s) 260, 326, 456
BseGI GGATG 3 cut(s) 456, 628, 647
BseJI GATNNNNATC 1 cut(s) 646
BseLI CCNNNNNNNGG 3 cut(s) 425, 462, 463
BseMI GCAATG 2 cut(s) 376, 447
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 3 cut(s) 401, 401, 591
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 259, 520
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 9, 400
BshNI GGYRCC 1 cut(s) 256
BsiEI CGRYCG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 458
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 3 cut(s) 425, 462, 463
BsmAI GTCTC 2 cut(s) 98, 579
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 2 cut(s) 9, 400
Bsp1286I GDGCHC 1 cut(s) 334
Bsp1407I TGTACA 1 cut(s) 676
Bsp143I GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
BspANI GGCC 2 cut(s) 9, 400
BspCNI CTCAG 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 258, 391, 480
BspPI GGATC 4 cut(s) 169, 228, 574, 649
BspT107I GGYRCC 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 376, 447
BsrGI TGTACA 1 cut(s) 676
BsrI ACTGG 3 cut(s) 401, 401, 591
BssECI CCNNGG 3 cut(s) 260, 326, 456
BssMI GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
BssNAI GTATAC 1 cut(s) 522
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 326
Bst1107I GTATAC 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 2 cut(s) 261, 579
Bst4CI ACNGT 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 676
BstC8I GCNNGC 2 cut(s) 243, 544
BstDEI CTNAG 2 cut(s) 109, 333
BstF5I GGATG 3 cut(s) 456, 628, 647
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 8 cut(s) 160, 177, 223, 316, 341, 562, 569, 644
BstMAI GTCTC 2 cut(s) 98, 579
BstMBI GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 146, 247, 539
BstNI CCWGG 2 cut(s) 261, 579
BstNSI RCATGY 4 cut(s) 40, 187, 245, 546
BstSCI CCNGG 3 cut(s) 259, 456, 577
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 259, 520
BstX2I RGATCY 3 cut(s) 338, 566, 641
BstYI RGATCY 3 cut(s) 338, 566, 641
BstZ17I GTATAC 1 cut(s) 522
BsuRI GGCC 2 cut(s) 9, 400
BtsCI GGATG 3 cut(s) 456, 628, 647
BtsIMutI CAGTG 2 cut(s) 509, 562
Cac8I GCNNGC 2 cut(s) 243, 544
CaiI CAGNNNCTG 1 cut(s) 253
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 665
Csp6I GTAC 3 cut(s) 305, 602, 677
CviAII CATG 6 cut(s) 37, 146, 184, 242, 543, 618
CviQI GTAC 3 cut(s) 305, 602, 677
DdeI CTNAG 2 cut(s) 109, 333
DpnI GATC 8 cut(s) 159, 176, 222, 315, 340, 561, 568, 643
DpnII GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
EaeI YGGCCR 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 116, 285
EcoRII CCWGG 2 cut(s) 259, 577
EcoT14I CCWWGG 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 6 cut(s) 40, 149, 187, 245, 546, 621
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 277
FatI CATG 6 cut(s) 36, 145, 183, 241, 542, 617
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 2 cut(s) 408, 521
Fnu4HI GCNGC 2 cut(s) 248, 534
FokI GGATG 3 cut(s) 463, 634, 635
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 2 cut(s) 248, 534
FspBI CTAG 1 cut(s) 287
FspI TGCGCA 1 cut(s) 246
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 248, 534
HaeIII GGCC 2 cut(s) 9, 400
HapII CCGG 1 cut(s) 458
HgaI GACGC 1 cut(s) 665
HhaI GCGC 1 cut(s) 247
Hin1II CATG 6 cut(s) 40, 149, 187, 245, 546, 621
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 4 cut(s) 191, 228, 276, 506
HpaII CCGG 1 cut(s) 458
Hpy166II GTNNAC 4 cut(s) 284, 409, 522, 677
Hpy188I TCNGA 5 cut(s) 118, 336, 394, 487, 501
Hpy188III TCNNGA 2 cut(s) 232, 551
Hpy8I GTNNAC 4 cut(s) 284, 409, 522, 677
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4V TGCA 2 cut(s) 542, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 247, 539
HpyF3I CTNAG 2 cut(s) 109, 333
Hsp92II CATG 6 cut(s) 40, 149, 187, 245, 546, 621
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 2 cut(s) 259, 520
LweI GCATC 4 cut(s) 122, 259, 441, 656
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 1 cut(s) 354
MalI GATC 8 cut(s) 159, 176, 222, 315, 340, 561, 568, 643
MboI GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
MboII GAAGA 1 cut(s) 237
MflI RGATCY 3 cut(s) 338, 566, 641
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 2 cut(s) 61, 377
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 2 cut(s) 200, 500
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 5 cut(s) 66, 107, 188, 210, 632
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 4 cut(s) 33, 48, 414, 432
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 400
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 1 cut(s) 458
MspR9I CCNGG 3 cut(s) 261, 458, 579
MvaI CCWGG 2 cut(s) 261, 579
MwoI GCNNNNNNNGC 3 cut(s) 146, 247, 539
NciI CCSGG 1 cut(s) 458
NdeII GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
NlaIII CATG 6 cut(s) 40, 149, 187, 245, 546, 621
NlaIV GGNNCC 3 cut(s) 258, 391, 480
NmuCI GTSAC 1 cut(s) 354
NsbI TGCGCA 1 cut(s) 246
NspI RCATGY 4 cut(s) 40, 187, 245, 546
PaeI GCATGC 2 cut(s) 245, 546
PciI ACATGT 1 cut(s) 183
PfeI GAWTC 2 cut(s) 228, 276
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 2 cut(s) 249, 535
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 2 cut(s) 199, 500
PpsI GAGTC 2 cut(s) 199, 500
PscI ACATGT 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 414
Psp6I CCWGG 2 cut(s) 259, 577
PspGI CCWGG 2 cut(s) 259, 577
PspN4I GGNNCC 3 cut(s) 258, 391, 480
PspPI GGNCC 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 253
PsuI RGATCY 3 cut(s) 338, 566, 641
PvuI CGATCG 1 cut(s) 160
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 3 cut(s) 306, 603, 678
RsaNI GTAC 3 cut(s) 305, 602, 677
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 4 cut(s) 33, 48, 414, 432
SatI GCNGC 2 cut(s) 248, 534
Sau3AI GATC 8 cut(s) 157, 174, 220, 313, 338, 559, 566, 641
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 2 cut(s) 200, 500
ScrFI CCNGG 3 cut(s) 261, 458, 579
SduI GDGCHC 1 cut(s) 334
SetI ASST 7 cut(s) 105, 110, 138, 262, 312, 427, 535
SfaNI GCATC 4 cut(s) 122, 259, 441, 656
SfcI CTRYAG 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 63
SphI GCATGC 2 cut(s) 245, 546
Sse9I AATT 2 cut(s) 61, 377
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 3 cut(s) 259, 456, 577
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 1 cut(s) 557
TaqI TCGA 3 cut(s) 160, 180, 223
TasI AATT 2 cut(s) 61, 377
TatI WGTACW 2 cut(s) 304, 676
TfiI GAWTC 2 cut(s) 228, 276
Tru1I TTAA 4 cut(s) 33, 48, 414, 432
Tru9I TTAA 4 cut(s) 33, 48, 414, 432
TscAI CASTG 2 cut(s) 516, 562
TseFI GTSAC 1 cut(s) 354
TseI GCWGC 2 cut(s) 247, 533
Tsp45I GTSAC 1 cut(s) 354
TspDTI ATGAA 4 cut(s) 102, 134, 458, 606
TspGWI ACGGA 2 cut(s) 225, 678
TspRI CASTG 2 cut(s) 516, 562
VspI ATTAAT 1 cut(s) 414
XceI RCATGY 4 cut(s) 40, 187, 245, 546
XmiI GTMKAC 2 cut(s) 408, 521
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.