Rmu_sc0004118.1_g000006
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004118.1
N/A
Physical Location & Seq
Reverse (-)
39034 .. 40337
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 378 bp
atgcgtgcgttacttgcggggcaagtgtaccataacgcgcaccagccgaagtacccgcctgaggccacctataaatacctgagtgaaaacaagtatgtgaggacgacaacatcaagactttcgctcccgctccagtcagaggaagtagactggttgtacagtgtgctcgcttccctcggtctgtggcagaaggaggccaagatcttattcctagtgctcaataatgctggcaagaccaccttgcttcacatgctcaaagatgagaggctggtgcagcatcaacctacgcaatatcccacattcgaggagttgagaattggcaaaatcaagttcaaggcctttgatttgggaggttatcagattgcttgcagggtctag

Protein Analysis

125

Amino Acids

14.47

Weight (kDa)

9.35

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 53 - 121 1.8e-20 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 147
AccII CGCG 1 cut(s) 38
AciI CCGC 3 cut(s) 17, 56, 128
AfaI GTAC 3 cut(s) 29, 53, 158
AfiI CCNNNNNNNGG 2 cut(s) 61, 139
AgsI TTSAA 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
Alw21I GWGCWC 2 cut(s) 168, 219
AoxI GGCC 3 cut(s) 63, 195, 336
ApeKI GCWGC 1 cut(s) 274
AspLEI GCGC 1 cut(s) 40
AxyI CCTNAGG 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 168, 219
BbvI GCAGC 1 cut(s) 286
BfaI CTAG 2 cut(s) 212, 376
BglII AGATCT 1 cut(s) 201
BisI GCNGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 276
BmsI GCATC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 139
Bse1I ACTGG 2 cut(s) 133, 155
Bse21I CCTNAGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 61, 139
BseMII CTCAG 2 cut(s) 51, 71
BseNI ACTGG 2 cut(s) 133, 155
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 65, 197, 338
BsiHKAI GWGCWC 2 cut(s) 168, 219
BslI CCNNNNNNNGG 2 cut(s) 61, 139
BsnI GGCC 3 cut(s) 65, 197, 338
Bsp1286I GDGCHC 2 cut(s) 168, 219
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 201
BspACI CCGC 3 cut(s) 17, 56, 128
BspANI GGCC 3 cut(s) 65, 197, 338
BspCNI CTCAG 2 cut(s) 52, 72
BspFNI CGCG 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 133, 155
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 4 cut(s) 6, 168, 229, 367
BstDEI CTNAG 2 cut(s) 60, 80
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 3 cut(s) 14, 250, 274
BstNSI RCATGY 1 cut(s) 253
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 65, 197, 338
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 4 cut(s) 6, 168, 229, 367
CfoI GCGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 28, 52, 157
CviAII CATG 1 cut(s) 250
CviJI RGCY 5 cut(s) 46, 65, 197, 268, 338
CviKI_1 RGCY 5 cut(s) 46, 65, 197, 268, 338
CviQI GTAC 3 cut(s) 28, 52, 157
DdeI CTNAG 2 cut(s) 60, 80
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
Eco147I AGGCCT 1 cut(s) 338
Eco81I CCTNAGG 1 cut(s) 60
FaeI CATG 1 cut(s) 253
FaiI YATR 4 cut(s) 33, 72, 96, 251
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FatI CATG 1 cut(s) 249
FauI CCCGC 3 cut(s) 10, 63, 135
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 275
FspBI CTAG 2 cut(s) 212, 376
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 275
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 3 cut(s) 65, 197, 338
HhaI GCGC 1 cut(s) 40
Hin1II CATG 1 cut(s) 253
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 28, 148
Hpy188I TCNGA 2 cut(s) 139, 360
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 2 cut(s) 28, 148
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4V TGCA 2 cut(s) 274, 369
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 250, 274
HpyF3I CTNAG 2 cut(s) 60, 80
Hsp92II CATG 1 cut(s) 253
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 2 cut(s) 129, 135
LpnPI CCDG 8 cut(s) 56, 72, 92, 136, 146, 213, 254, 355
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 286
MaeI CTAG 2 cut(s) 212, 376
MaeIII GTNAC 1 cut(s) 9
MalI GATC 1 cut(s) 203
MbiI CCGCTC 1 cut(s) 130
MboI GATC 1 cut(s) 201
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 168, 219
MluCI AATT 1 cut(s) 315
MnlI CCTC 8 cut(s) 55, 93, 133, 185, 187, 258, 298, 344
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 14, 250, 274
NdeII GATC 1 cut(s) 201
NlaIII CATG 1 cut(s) 253
NspI RCATGY 1 cut(s) 253
PceI AGGCCT 1 cut(s) 338
PkrI GCNGC 1 cut(s) 276
PsuI RGATCY 1 cut(s) 201
RsaI GTAC 3 cut(s) 29, 53, 158
RsaNI GTAC 3 cut(s) 28, 52, 157
SatI GCNGC 1 cut(s) 275
Sau3AI GATC 1 cut(s) 201
SduI GDGCHC 2 cut(s) 168, 219
SetI ASST 5 cut(s) 71, 81, 242, 286, 355
SfaNI GCATC 1 cut(s) 286
Sse9I AATT 1 cut(s) 315
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 3 cut(s) 17, 56, 128
SspMI CTAG 2 cut(s) 212, 376
StuI AGGCCT 1 cut(s) 338
TaaI ACNGT 1 cut(s) 161
TaqI TCGA 1 cut(s) 303
TaqII GACCGA 1 cut(s) 167
TasI AATT 1 cut(s) 315
TatI WGTACW 1 cut(s) 156
TscAI CASTG 1 cut(s) 166
TseI GCWGC 1 cut(s) 274
TspRI CASTG 1 cut(s) 166
XceI RCATGY 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 212, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.