Rmu_sc0004206.1_g000016
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004206.1
N/A
Physical Location & Seq
Forward (+)
68158 .. 74064
5907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
atgaaaaaccaaaaacaaaatgatccaagtggaagtcagagtcgctcgaacctgagtttctcggttcagacatcttcatcggagtcggacgtgcaagccgagccggccccggcggtgaagaagttcggcgagattgaagaggtggaacaaccagatgaagaggcggcgatcagagctcaacagatctgggtcggaggtccgagctgcagaacatcttcaccacatcatgacggtggcgattccggagaaaccctctacggttggactcacttgtctaaatggctcttacatgactcagagcttactctagctccgatgttcttctctgaaaataccgagttcaaccgattgttcaacgatggcatggcttgcgccgctaaaatcacaataaaagcgatactagtgggatataagggtgggtttgataacgtggcgacactggtggatgttggtggtgggactggaggcgcagtggcggagattatgaagttgtatcccagcattaagggcatcaactttgatctaccacatgtagttgcaacagcgccggtataccatggggtgtcacatgttggatcgagcaggtggctttatcatcagagatcaaagaggaattccgatacgcttgttgcagcaaccagaagctttgggactaccagtgtaccattagctgaagcaatggctctaaggaacagtcttgtacttgccgtcttgccgaacaatatggattcaccaatatacaagcagaaggagactcaaatcggtccaaagaccagtctttcccaactctcaaacaccaaccccgtctcctcgtttagaacttcagatcagtttcaccttccactcttccaaagttccagcttccaggctccagccactgagccacctccaaggctccaatcgctgcgacccacagagccacctccaatcactgcgagccacggagccgggaaccacctccaatctcagagtttcagtccactgtgccacctcctactctcactctcctcgtctccccgttcagaacttcagatcaattttgattccaccttccagagttccacgctccagccaccccccttttcagagtttcagtctgcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

367

Amino Acids

39.73

Weight (kDa)

6.05

Isoelectric Point (pI)

71.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 110 - 185 1.6e-19 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 573
AasI GACNNNNNNGTC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 4 cut(s) 113, 164, 375, 476
AclWI GGATC 2 cut(s) 17, 583
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 3 cut(s) 693, 807, 1013
AfaI GTAC 2 cut(s) 663, 702
AflIII ACRYGT 2 cut(s) 529, 568
AgsI TTSAA 3 cut(s) 137, 343, 355
AhlI ACTAGT 1 cut(s) 400
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 1 cut(s) 864
AluBI AGCT 7 cut(s) 176, 204, 301, 311, 645, 671, 861
AluI AGCT 7 cut(s) 176, 204, 301, 311, 645, 671, 861
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 3 cut(s) 746, 811, 1017
AlwI GGATC 2 cut(s) 17, 583
AlwNI CAGNNNCTG 1 cut(s) 878
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 3 cut(s) 204, 632, 904
ApoI RAATTY 1 cut(s) 613
Asp700I GAANNNNTTC 2 cut(s) 122, 214
AspLEI GCGC 3 cut(s) 374, 470, 547
AspS9I GGNCC 3 cut(s) 106, 197, 764
AsuC2I CCSGG 2 cut(s) 110, 949
AsuHPI GGTGA 4 cut(s) 127, 210, 723, 827
AvaII GGWCC 2 cut(s) 197, 764
BaeI ACNNNNGTAYC 4 cut(s) 612, 645, 645, 678
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 191, 644, 891
BccI CCATC 1 cut(s) 353
BceAI ACGGC 1 cut(s) 692
BciT130I CCWGG 1 cut(s) 866
BciVI GTATCC 1 cut(s) 504
BcnI CCSGG 2 cut(s) 110, 949
BcoDI GTCTC 3 cut(s) 746, 811, 1017
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 2 cut(s) 308, 401
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 573
BfuI GTATCC 1 cut(s) 504
BglI GCCNNNNNGGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 183
BisI GCNGC 5 cut(s) 165, 205, 375, 633, 905
BlsI GCNGC 5 cut(s) 166, 206, 376, 634, 906
Bme1390I CCNGG 3 cut(s) 110, 866, 949
Bme18I GGWCC 2 cut(s) 197, 764
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 3 cut(s) 106, 197, 764
BmiI GGNNCC 5 cut(s) 108, 870, 896, 946, 953
BmrFI CCNGG 3 cut(s) 110, 866, 949
BmsI GCATC 1 cut(s) 519
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpmI CTGGAG 3 cut(s) 483, 855, 1052
BpuMI CCSGG 2 cut(s) 110, 949
BsaJI CCNNGG 4 cut(s) 108, 556, 890, 940
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 6 cut(s) 295, 325, 456, 486, 791, 821
Bse118I RCCGGY 2 cut(s) 103, 547
Bse1I ACTGG 4 cut(s) 444, 466, 657, 774
Bse3DI GCAATG 1 cut(s) 684
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 1 cut(s) 866
BseDI CCNNGG 4 cut(s) 108, 556, 890, 940
BseGI GGATG 1 cut(s) 451
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 4 cut(s) 44, 309, 870, 980
BseNI ACTGG 4 cut(s) 444, 466, 657, 774
BseRI GAGGAG 2 cut(s) 799, 997
BseXI GCAGC 3 cut(s) 191, 644, 891
BseYI CCCAGC 1 cut(s) 497
BshFI GGCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 5 cut(s) 104, 110, 243, 548, 948
BslFI GGGAC 2 cut(s) 472, 664
BsmAI GTCTC 3 cut(s) 746, 811, 1017
BsmBI CGTCTC 2 cut(s) 811, 1017
BsmFI GGGAC 2 cut(s) 472, 664
BsnI GGCC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 178
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
Bsp19I CCATGG 1 cut(s) 556
BspACI CCGC 4 cut(s) 113, 164, 375, 476
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 4 cut(s) 45, 308, 871, 979
BspEI TCCGGA 1 cut(s) 242
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 5 cut(s) 108, 870, 896, 946, 953
BspMAI CTGCAG 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 573
BspPI GGATC 2 cut(s) 17, 583
BsrDI GCAATG 1 cut(s) 684
BsrFI RCCGGY 2 cut(s) 103, 547
BsrI ACTGG 4 cut(s) 444, 466, 657, 774
BssAI RCCGGY 2 cut(s) 103, 547
BssECI CCNNGG 4 cut(s) 108, 556, 890, 940
BssMI GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
BssNAI GTATAC 1 cut(s) 553
BssT1I CCWWGG 2 cut(s) 556, 890
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 1 cut(s) 866
Bst4CI ACNGT 4 cut(s) 233, 260, 695, 984
Bst6I CTCTTC 3 cut(s) 132, 153, 851
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 4 cut(s) 96, 105, 370, 937
BstDEI CTNAG 5 cut(s) 53, 295, 686, 879, 966
BstDSI CCRYGG 2 cut(s) 556, 940
BstF5I GGATG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 3 cut(s) 374, 470, 547
BstKTI GATC 8 cut(s) 25, 171, 186, 523, 578, 605, 829, 1035
BstMAI GTCTC 3 cut(s) 746, 811, 1017
BstMBI GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
BstMWI GCNNNNNNNGC 7 cut(s) 100, 104, 173, 369, 374, 507, 901
BstNI CCWGG 1 cut(s) 866
BstNSI RCATGY 2 cut(s) 533, 572
BstSCI CCNGG 3 cut(s) 108, 864, 947
BstSFI CTRYAG 1 cut(s) 205
BstV1I GCAGC 3 cut(s) 191, 644, 891
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BstZ17I GTATAC 1 cut(s) 553
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 1 cut(s) 107
BtgI CCRYGG 2 cut(s) 556, 940
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 451
BtsI GCAGTG 2 cut(s) 477, 930
BtsIMutI CAGTG 6 cut(s) 437, 477, 664, 876, 930, 980
BveI ACCTGC 1 cut(s) 573
Cac8I GCNNGC 4 cut(s) 96, 105, 370, 937
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 1 cut(s) 226
CfoI GCGC 3 cut(s) 374, 470, 547
Cfr10I RCCGGY 2 cut(s) 103, 547
Cfr13I GGNCC 3 cut(s) 106, 197, 764
Csp6I GTAC 2 cut(s) 662, 701
CviAII CATG 6 cut(s) 227, 290, 364, 530, 557, 569
CviQI GTAC 2 cut(s) 662, 701
DdeI CTNAG 5 cut(s) 53, 295, 686, 879, 966
DpnI GATC 8 cut(s) 24, 170, 185, 522, 577, 604, 828, 1034
DpnII GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 132, 153, 851
EarI CTCTTC 3 cut(s) 132, 153, 851
EciI GGCGGA 1 cut(s) 491
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 556, 890
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 197, 764
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 3 cut(s) 693, 807, 1013
EcoICRI GAGCTC 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 2 cut(s) 556, 890
EcoT38I GRGCYC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 556, 890
Esp3I CGTCTC 2 cut(s) 811, 1017
FaeI CATG 6 cut(s) 230, 293, 367, 533, 560, 572
FaqI GGGAC 2 cut(s) 472, 664
FatI CATG 6 cut(s) 226, 289, 363, 529, 556, 568
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 165, 205, 375, 633, 905
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 5 cut(s) 165, 205, 375, 633, 905
FspBI CTAG 2 cut(s) 308, 401
GlaI GCGC 3 cut(s) 373, 469, 546
GluI GCNGC 5 cut(s) 165, 205, 375, 633, 905
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 3 cut(s) 483, 855, 1052
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 1 cut(s) 107
HapII CCGG 5 cut(s) 104, 110, 243, 548, 948
HhaI GCGC 3 cut(s) 374, 470, 547
Hin1II CATG 6 cut(s) 230, 293, 367, 533, 560, 572
Hin6I GCGC 3 cut(s) 372, 468, 545
HinP1I GCGC 3 cut(s) 372, 468, 545
HindIII AAGCTT 1 cut(s) 643
HinfI GANTC 8 cut(s) 40, 83, 239, 265, 293, 728, 754, 1043
HpaII CCGG 5 cut(s) 104, 110, 243, 548, 948
HphI GGTGA 4 cut(s) 127, 210, 723, 827
Hpy166II GTNNAC 3 cut(s) 553, 662, 980
Hpy188III TCNNGA 3 cut(s) 227, 243, 1054
Hpy8I GTNNAC 3 cut(s) 553, 662, 980
HpyAV CCTTC 3 cut(s) 742, 848, 1060
HpyCH4III ACNGT 4 cut(s) 233, 260, 695, 984
HpyCH4IV ACGT 2 cut(s) 90, 429
HpyCH4V TGCA 4 cut(s) 94, 207, 539, 632
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 104, 173, 369, 374, 507, 901
HpyF3I CTNAG 5 cut(s) 53, 295, 686, 879, 966
HpySE526I ACGT 2 cut(s) 90, 429
Hsp92II CATG 6 cut(s) 230, 293, 367, 533, 560, 572
HspAI GCGC 3 cut(s) 372, 468, 545
Kpn2I TCCGGA 1 cut(s) 242
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
LmnI GCTCC 5 cut(s) 316, 874, 900, 944, 1071
Lsp1109I GCAGC 3 cut(s) 191, 644, 891
LweI GCATC 1 cut(s) 519
MaeI CTAG 2 cut(s) 308, 401
MaeII ACGT 2 cut(s) 90, 429
MaeIII GTNAC 1 cut(s) 564
MalI GATC 8 cut(s) 24, 170, 185, 522, 577, 604, 828, 1034
MboI GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
MboII GAAGA 7 cut(s) 66, 130, 149, 170, 207, 313, 838
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 2 cut(s) 613, 1036
MlyI GAGTC 5 cut(s) 49, 92, 259, 287, 748
MmeI TCCRAC 4 cut(s) 66, 172, 242, 553
MroI TCCGGA 1 cut(s) 242
MroNI GCCGGC 1 cut(s) 103
MroXI GAANNNNTTC 2 cut(s) 122, 214
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 5 cut(s) 104, 110, 243, 548, 948
MspR9I CCNGG 3 cut(s) 110, 866, 949
MvaI CCWGG 1 cut(s) 866
MwoI GCNNNNNNNGC 7 cut(s) 100, 104, 173, 369, 374, 507, 901
NaeI GCCGGC 1 cut(s) 105
NciI CCSGG 2 cut(s) 110, 949
NcoI CCATGG 1 cut(s) 556
NdeII GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 6 cut(s) 230, 293, 367, 533, 560, 572
NlaIV GGNNCC 5 cut(s) 108, 870, 896, 946, 953
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 564
NspI RCATGY 2 cut(s) 533, 572
PagI TCATGA 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 573
PciI ACATGT 2 cut(s) 529, 568
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 105
PdmI GAANNNNTTC 2 cut(s) 122, 214
PfeI GAWTC 3 cut(s) 239, 728, 1043
PkrI GCNGC 5 cut(s) 166, 206, 376, 634, 906
PleI GAGTC 5 cut(s) 48, 91, 259, 287, 748
PpsI GAGTC 5 cut(s) 48, 91, 259, 287, 748
PscI ACATGT 2 cut(s) 529, 568
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 864
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 1 cut(s) 864
PspN4I GGNNCC 5 cut(s) 108, 870, 896, 946, 953
PspPI GGNCC 3 cut(s) 106, 197, 764
PstI CTGCAG 1 cut(s) 209
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 2 cut(s) 663, 702
RsaNI GTAC 2 cut(s) 662, 701
RseI CAYNNNNRTG 1 cut(s) 231
SacI GAGCTC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 5 cut(s) 165, 205, 375, 633, 905
Sau3AI GATC 8 cut(s) 22, 168, 183, 520, 575, 602, 826, 1032
Sau96I GGNCC 3 cut(s) 106, 197, 764
SchI GAGTC 5 cut(s) 49, 92, 259, 287, 748
ScrFI CCNGG 3 cut(s) 110, 866, 949
SduI GDGCHC 1 cut(s) 178
SfaNI GCATC 1 cut(s) 519
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 2 cut(s) 197, 764
SmiMI CAYNNNNRTG 1 cut(s) 231
SpeI ACTAGT 1 cut(s) 400
Sse9I AATT 2 cut(s) 613, 1036
SsiI CCGC 4 cut(s) 113, 164, 375, 476
SspMI CTAG 2 cut(s) 308, 401
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 3 cut(s) 108, 864, 947
StyI CCWWGG 2 cut(s) 556, 890
TaaI ACNGT 4 cut(s) 233, 260, 695, 984
TaiI ACGT 2 cut(s) 93, 432
TaqI TCGA 2 cut(s) 47, 578
TaqII GACCGA 1 cut(s) 752
TasI AATT 2 cut(s) 613, 1036
TatI WGTACW 1 cut(s) 700
TauI GCSGC 2 cut(s) 167, 377
TfiI GAWTC 3 cut(s) 239, 728, 1043
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 6 cut(s) 444, 477, 664, 883, 937, 987
TseFI GTSAC 1 cut(s) 564
TseI GCWGC 3 cut(s) 204, 632, 904
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 4 cut(s) 17, 66, 171, 500
TspGWI ACGGA 1 cut(s) 957
TspRI CASTG 6 cut(s) 444, 477, 664, 883, 937, 987
VpaK11BI GGWCC 2 cut(s) 197, 764
XapI RAATTY 1 cut(s) 613
XceI RCATGY 2 cut(s) 533, 572
XmiI GTMKAC 1 cut(s) 552
XmnI GAANNNNTTC 2 cut(s) 122, 214
XspI CTAG 2 cut(s) 308, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.