Rmu_sc0004244.1_g000008

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004244.1
N/A
Physical Location & Seq
Forward (+)
42091 .. 45484
3394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
atggaggcaaatggtatcttgatcggcgaacaaggttatgaccgagctgaagaagtgaagcaattcgacgactccaagcttgaagtcaaaggccttgtcgactcgggtctctcctctatccccagaatctttatccaaccgcccaaaatattacagaaattccagcctacgacccgacccaacgcccaaccaatccccaccatcgacctctctgaccttcgccgctcctccagcgttgaccaagttagaggcgcgtgccgaaacttcagtttcttccagatcaccaaccacggcataccatcggtggttctcgactatacgatcgccgctgtcaaggccttccacgagcaaccggaaggagaggtggaagagctgggagagggtttagttagaggaaagggcgtcggggtggtggagttagagggagagggagtccgagtcgggttcgggttcggaggggcagcaaagaaggagaggaggagagtgaaggtgagggaaaagaagattgagatagacaatgccggcgatgttgcttcgcgttctcaccgctactctctgatgccccaatatcctctctcttcaacacccaactctctcctccggccatgtaatccccgaccccttattttcgttcaccctcccgtcactctatcatcgcctcatatcctcctcctcctcctccccttccacccaaaaccctccgaactcattcaaaacccaaaccccactagagaagcaattcgaaacatgggtccagaaactcaaacccatatatggtccacccgatgtcgacgaggccatgaaatcctaatcggatctcgaccactccacctacctcaccatgatcaaaatcctaatcaacggtcggcgggaactttaagactgtttcggaggctattcaggcagcttccggggccagtttttgatttatgtgaagccgggagtcttacgactaaaaaagatggcgacggcaaatcttctatcgccgttggtggcgtcgccaatgctatttgctacgacaaatgcgccgtcgtaagagtattagcgacggcaaatcttctatcgccgttggtggcgtcgccaatgctatttgctatggcaaatgcgccgtcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

371

Amino Acids

40.75

Weight (kDa)

9.33

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 121 1.6e-11 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 2 cut(s) 99, 790
AccII CGCG 2 cut(s) 254, 538
AciI CCGC 5 cut(s) 140, 223, 327, 547, 869
AclWI GGATC 1 cut(s) 823
AcoI YGGCCR 1 cut(s) 602
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 2 cut(s) 69, 250
AcyI GRCGYC 3 cut(s) 402, 996, 1076
AfiI CCNNNNNNNGG 1 cut(s) 774
AgsI TTSAA 3 cut(s) 83, 582, 713
AluBI AGCT 4 cut(s) 47, 79, 373, 907
AluI AGCT 4 cut(s) 47, 79, 373, 907
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 1 cut(s) 823
Ama87I CYCGRG 1 cut(s) 103
AoxI GGCC 5 cut(s) 91, 336, 602, 796, 914
ApeKI GCWGC 2 cut(s) 461, 904
ApoI RAATTY 1 cut(s) 158
ArsI GACNNNNNNTTYG 7 cut(s) 81, 113, 736, 768, 1014, 1046, 1094
Asp700I GAANNNNTTC 3 cut(s) 62, 708, 738
AspLEI GCGC 3 cut(s) 254, 1028, 1108
AspS9I GGNCC 3 cut(s) 752, 777, 914
AsuC2I CCSGG 2 cut(s) 912, 940
AsuHPI GGTGA 5 cut(s) 274, 502, 536, 626, 830
AsuII TTCGAA 1 cut(s) 742
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 2 cut(s) 752, 777
BauI CACGAG 1 cut(s) 344
BbvI GCAGC 2 cut(s) 473, 916
BccI CCATC 3 cut(s) 209, 307, 956
BceAI ACGGC 7 cut(s) 307, 971, 985, 1013, 1051, 1065, 1093
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 2 cut(s) 912, 940
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 1 cut(s) 729
BisI GCNGC 4 cut(s) 223, 327, 462, 905
BlsI GCNGC 4 cut(s) 224, 328, 463, 906
Bme1390I CCNGG 2 cut(s) 912, 940
Bme18I GGWCC 2 cut(s) 752, 777
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 752, 777, 914
BmiI GGNNCC 2 cut(s) 753, 915
BmrFI CCNGG 2 cut(s) 912, 940
BmsI GCATC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 214
Bpu14I TTCGAA 1 cut(s) 742
BpuMI CCSGG 2 cut(s) 912, 940
BsaBI GATNNNNATC 1 cut(s) 849
BsaHI GRCGYC 3 cut(s) 402, 996, 1076
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 289, 911
BsaWI WCCGGW 1 cut(s) 352
BsaXI ACNNNNNCTCC 4 cut(s) 417, 423, 447, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 774
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 1 cut(s) 917
Bse8I GATNNNNATC 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 289, 911
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 1 cut(s) 774
BseNI ACTGG 1 cut(s) 917
BseRI GAGGAG 9 cut(s) 103, 217, 490, 493, 587, 659, 662, 665, 668
BseXI GCAGC 2 cut(s) 473, 916
BseYI CCCAGC 1 cut(s) 373
Bsh1236I CGCG 2 cut(s) 254, 538
Bsh1285I CGRYCG 2 cut(s) 324, 866
BshFI GGCC 5 cut(s) 93, 338, 604, 798, 916
BsiEI CGRYCG 2 cut(s) 324, 866
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 5 cut(s) 353, 522, 601, 911, 939
BslI CCNNNNNNNGG 1 cut(s) 774
BsmAI GTCTC 1 cut(s) 113
BsnI GGCC 5 cut(s) 93, 338, 604, 798, 916
Bso31I GGTCTC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 742
Bsp143I GATC 5 cut(s) 21, 279, 321, 815, 844
BspACI CCGC 5 cut(s) 140, 223, 327, 547, 869
BspANI GGCC 5 cut(s) 93, 338, 604, 798, 916
BspFNI CGCG 2 cut(s) 254, 538
BspLI GGNNCC 2 cut(s) 753, 915
BspPI GGATC 1 cut(s) 823
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 1 cut(s) 742
BspTNI GGTCTC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 1 cut(s) 917
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 289, 911
BssMI GATC 5 cut(s) 21, 279, 321, 815, 844
BssNI GRCGYC 3 cut(s) 402, 996, 1076
BssSI CACGAG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 864, 885
Bst6I CTCTTC 2 cut(s) 363, 583
BstACI GRCGYC 3 cut(s) 402, 996, 1076
BstBI TTCGAA 1 cut(s) 742
BstC8I GCNNGC 2 cut(s) 256, 523
BstDSI CCRYGG 1 cut(s) 289
BstFNI CGCG 2 cut(s) 254, 538
BstHHI GCGC 3 cut(s) 254, 1028, 1108
BstKTI GATC 5 cut(s) 24, 282, 324, 818, 847
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 5 cut(s) 21, 279, 321, 815, 844
BstMCI CGRYCG 2 cut(s) 324, 866
BstMWI GCNNNNNNNGC 4 cut(s) 231, 335, 901, 913
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 910, 938
BstUI CGCG 2 cut(s) 254, 538
BstV1I GCAGC 2 cut(s) 473, 916
BstX2I RGATCY 1 cut(s) 815
BstYI RGATCY 1 cut(s) 815
BsuRI GGCC 5 cut(s) 93, 338, 604, 798, 916
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 2 cut(s) 540, 639
Cac8I GCNNGC 2 cut(s) 256, 523
CfoI GCGC 3 cut(s) 254, 1028, 1108
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 3 cut(s) 752, 777, 914
CseI GACGC 3 cut(s) 391, 985, 1065
CviAII CATG 4 cut(s) 606, 748, 800, 842
DpnI GATC 5 cut(s) 23, 281, 323, 817, 846
DpnII GATC 5 cut(s) 21, 279, 321, 815, 844
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 2 cut(s) 363, 583
EarI CTCTTC 2 cut(s) 363, 583
Eco147I AGGCCT 2 cut(s) 93, 338
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 752, 777
Eco57I CTGAAG 2 cut(s) 69, 250
Eco88I CYCGRG 1 cut(s) 103
FaeI CATG 4 cut(s) 609, 751, 803, 845
FatI CATG 4 cut(s) 605, 747, 799, 841
FauI CCCGC 1 cut(s) 862
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 2 cut(s) 99, 790
Fnu4HI GCNGC 4 cut(s) 223, 327, 462, 905
Fsp4HI GCNGC 4 cut(s) 223, 327, 462, 905
FspBI CTAG 1 cut(s) 729
GlaI GCGC 3 cut(s) 253, 1027, 1107
GluI GCNGC 4 cut(s) 223, 327, 462, 905
GsaI CCCAGC 1 cut(s) 377
GsuI CTGGAG 1 cut(s) 214
HaeIII GGCC 5 cut(s) 93, 338, 604, 798, 916
HapII CCGG 5 cut(s) 353, 522, 601, 911, 939
HgaI GACGC 3 cut(s) 391, 985, 1065
HhaI GCGC 3 cut(s) 254, 1028, 1108
Hin1I GRCGYC 3 cut(s) 402, 996, 1076
Hin1II CATG 4 cut(s) 609, 751, 803, 845
Hin6I GCGC 3 cut(s) 252, 1026, 1106
HinP1I GCGC 3 cut(s) 252, 1026, 1106
HincII GTYRAC 3 cut(s) 100, 238, 791
HindII GTYRAC 3 cut(s) 100, 238, 791
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 6 cut(s) 71, 101, 126, 432, 438, 943
HpaII CCGG 5 cut(s) 353, 522, 601, 911, 939
HphI GGTGA 5 cut(s) 274, 502, 536, 626, 830
Hpy166II GTNNAC 5 cut(s) 100, 238, 634, 780, 791
Hpy188I TCNGA 7 cut(s) 214, 437, 455, 558, 703, 815, 891
Hpy188III TCNNGA 5 cut(s) 19, 277, 311, 755, 819
Hpy8I GTNNAC 5 cut(s) 100, 238, 634, 780, 791
Hpy99I CGWCG 9 cut(s) 71, 407, 795, 971, 1001, 1034, 1051, 1081, 1114
HpyAV CCTTC 6 cut(s) 227, 349, 350, 463, 481, 694
HpyCH4III ACNGT 2 cut(s) 864, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 335, 901, 913
Hsp92I GRCGYC 3 cut(s) 402, 996, 1076
Hsp92II CATG 4 cut(s) 609, 751, 803, 845
HspAI GCGC 3 cut(s) 252, 1026, 1106
KroI GCCGGC 1 cut(s) 521
KroNI GCCGGC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 5 cut(s) 21, 279, 321, 815, 844
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 2 cut(s) 473, 916
LweI GCATC 1 cut(s) 549
MaeI CTAG 1 cut(s) 729
MaeIII GTNAC 1 cut(s) 643
MalI GATC 5 cut(s) 23, 281, 323, 817, 846
MbiI CCGCTC 1 cut(s) 225
MboI GATC 5 cut(s) 21, 279, 321, 815, 844
MboII GAAGA 7 cut(s) 62, 265, 380, 514, 570, 969, 1049
MflI RGATCY 1 cut(s) 815
MluCI AATT 3 cut(s) 62, 158, 738
MlyI GAGTC 5 cut(s) 65, 95, 441, 447, 952
MmeI TCCRAC 1 cut(s) 160
MroNI GCCGGC 1 cut(s) 521
MroXI GAANNNNTTC 3 cut(s) 62, 708, 738
MseI TTAA 1 cut(s) 878
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 5 cut(s) 353, 522, 601, 911, 939
MspR9I CCNGG 2 cut(s) 912, 940
MvnI CGCG 2 cut(s) 254, 538
MwoI GCNNNNNNNGC 4 cut(s) 231, 335, 901, 913
NaeI GCCGGC 1 cut(s) 523
NciI CCSGG 2 cut(s) 912, 940
NdeII GATC 5 cut(s) 21, 279, 321, 815, 844
NgoMIV GCCGGC 1 cut(s) 521
NlaIII CATG 4 cut(s) 609, 751, 803, 845
NlaIV GGNNCC 2 cut(s) 753, 915
NmuCI GTSAC 1 cut(s) 643
NspV TTCGAA 1 cut(s) 742
PceI AGGCCT 2 cut(s) 93, 338
PciSI GCTCTTC 1 cut(s) 363
PdiI GCCGGC 1 cut(s) 523
PdmI GAANNNNTTC 3 cut(s) 62, 708, 738
PfeI GAWTC 1 cut(s) 126
PkrI GCNGC 4 cut(s) 224, 328, 463, 906
Ple19I CGATCG 1 cut(s) 324
PleI GAGTC 5 cut(s) 65, 95, 440, 446, 951
PpsI GAGTC 5 cut(s) 65, 95, 440, 446, 951
PshAI GACNNNNGTC 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 373
PspN4I GGNNCC 2 cut(s) 753, 915
PspPI GGNCC 3 cut(s) 752, 777, 914
PsuI RGATCY 1 cut(s) 815
PvuI CGATCG 1 cut(s) 324
SalI GTCGAC 2 cut(s) 98, 789
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 1 cut(s) 878
SatI GCNGC 4 cut(s) 223, 327, 462, 905
Sau3AI GATC 5 cut(s) 21, 279, 321, 815, 844
Sau96I GGNCC 3 cut(s) 752, 777, 914
SchI GAGTC 5 cut(s) 65, 95, 441, 447, 952
ScrFI CCNGG 2 cut(s) 912, 940
SfaNI GCATC 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 742
SinI GGWCC 2 cut(s) 752, 777
Sse9I AATT 3 cut(s) 62, 158, 738
SseBI AGGCCT 2 cut(s) 93, 338
SsiI CCGC 5 cut(s) 140, 223, 327, 547, 869
SspI AATATT 1 cut(s) 150
SspMI CTAG 1 cut(s) 729
StuI AGGCCT 2 cut(s) 93, 338
StyD4I CCNGG 2 cut(s) 910, 938
TaaI ACNGT 2 cut(s) 864, 885
TaqI TCGA 7 cut(s) 66, 99, 204, 312, 742, 790, 820
TaqII GACCGA 1 cut(s) 57
TasI AATT 3 cut(s) 62, 158, 738
TauI GCSGC 2 cut(s) 225, 329
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 1 cut(s) 878
Tru9I TTAA 1 cut(s) 878
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 2 cut(s) 461, 904
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 1 cut(s) 816
VpaK11BI GGWCC 2 cut(s) 752, 777
XapI RAATTY 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 99, 790
XmnI GAANNNNTTC 3 cut(s) 62, 708, 738
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.