Rmu_sc0004655.1_g000018

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004655.1
N/A
Physical Location & Seq
Forward (+)
55883 .. 59291
3409 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
atgcctaggcctcatggttttcggcccttgctaccacttaggagccctactactatgaggtatgttctccggctcattgatgtggctaatggttgttggaatgtccctttattggagagtttattccctagtgaggatgtggaggttattagctcaattcctttgagctctagatgtcctgagaacaaactgatttggcattatgatagtaagggagttttttcagtgagtagtgcttatgttgtggctagagacagtttgatcctcccagtcatggctacttctagctcatctcctcatccgcttattgataccttttggaagaagttttggcagtgcaaggtgccgggagatactgtccacctctcttttgttgtaattaaggttgatgcatcttggcattatactcaagacggtgttgatctcctggtgaaggggccttctggtgagcagatacatgagtttcgtgataagacaagcgagaaattcgagtttgtcactcatcacaaaggaatccatcagttttgtttcaccaacaagtcaccttatcatgaaaccattgactttgacttacatgtcagccattttacatactatgatcagcatgcaagagatgagcattttaaccctttgttagatcagataacaaagttagaggaagctctttacaacattcagtttgagcagcattggcttgaggctcagactgaacgccaggcaatagtgaacgacgcaatgagccggagggcgattcacaaggccttttttgaatcagcaggacttgttggtgccagtgttctccaagtttaccttcttcgccgcctctttgaacggaagcttgggacgtccagagtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

285

Amino Acids

32.91

Weight (kDa)

6.59

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 117 - 277 3.9e-29 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AatII GACGTC 1 cut(s) 848
AccB1I GGYRCC 2 cut(s) 343, 788
AciI CCGC 2 cut(s) 302, 820
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 845
AfiI CCNNNNNNNGG 4 cut(s) 112, 133, 274, 433
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 2 cut(s) 770, 830
AjnI CCWGG 2 cut(s) 426, 714
AjuI GAANNNNNNNTTGG 4 cut(s) 795, 822, 827, 854
AluBI AGCT 5 cut(s) 153, 168, 288, 662, 838
AluI AGCT 5 cut(s) 153, 168, 288, 662, 838
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 855
AoxI GGCC 4 cut(s) 8, 23, 437, 759
ApeKI GCWGC 1 cut(s) 685
ApoI RAATTY 1 cut(s) 485
AspA2I CCTAGG 1 cut(s) 5
AspS9I GGNCC 2 cut(s) 24, 437
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 4 cut(s) 442, 458, 523, 534
AvrII CCTAGG 1 cut(s) 5
BaeI ACNNNNGTAYC 2 cut(s) 446, 479
BanI GGYRCC 2 cut(s) 343, 788
BanII GRGCYC 2 cut(s) 47, 170
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 697
BccI CCATC 1 cut(s) 525
BciT130I CCWGG 2 cut(s) 428, 716
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 5 cut(s) 6, 129, 171, 249, 285
BisI GCNGC 2 cut(s) 686, 820
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 2 cut(s) 687, 821
Bme1390I CCNGG 3 cut(s) 348, 428, 716
BmgT120I GGNCC 2 cut(s) 24, 437
BmiI GGNNCC 4 cut(s) 44, 345, 438, 790
BmrFI CCNGG 3 cut(s) 348, 428, 716
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 2 cut(s) 379, 401
BmuI ACTGGG 1 cut(s) 263
BpuEI CTTGAG 2 cut(s) 393, 716
BpuMI CCSGG 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 845
BsaJI CCNNGG 1 cut(s) 5
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 133, 274, 433
Bse1I ACTGG 2 cut(s) 269, 792
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 2 cut(s) 428, 716
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 2 cut(s) 142, 298
BseLI CCNNNNNNNGG 4 cut(s) 112, 133, 274, 433
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 171, 716
BseNI ACTGG 2 cut(s) 269, 792
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 697
BshFI GGCC 4 cut(s) 10, 25, 439, 761
BshNI GGYRCC 2 cut(s) 343, 788
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 3 cut(s) 70, 347, 742
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 4 cut(s) 112, 133, 274, 433
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 4 cut(s) 10, 25, 439, 761
Bsp1286I GDGCHC 2 cut(s) 47, 170
Bsp143I GATC 4 cut(s) 261, 421, 598, 637
BspACI CCGC 2 cut(s) 302, 820
BspANI GGCC 4 cut(s) 10, 25, 439, 761
BspCNI CTCAG 2 cut(s) 172, 715
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 4 cut(s) 44, 345, 438, 790
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 2 cut(s) 343, 788
BsrDI GCAATG 1 cut(s) 741
BsrI ACTGG 2 cut(s) 269, 792
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 4 cut(s) 261, 421, 598, 637
BssNI GRCGYC 1 cut(s) 845
BssT1I CCWWGG 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 428, 716
Bst4CI ACNGT 3 cut(s) 257, 358, 416
BstACI GRCGYC 1 cut(s) 845
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 3 cut(s) 38, 180, 702
BstENI CCTNNNNNAGG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 142, 298
BstKTI GATC 4 cut(s) 264, 424, 601, 640
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 4 cut(s) 261, 421, 598, 637
BstMWI GCNNNNNNNGC 1 cut(s) 691
BstNI CCWGG 2 cut(s) 428, 716
BstNSI RCATGY 2 cut(s) 578, 608
BstSCI CCNGG 3 cut(s) 346, 426, 714
BstV1I GCAGC 1 cut(s) 697
BsuRI GGCC 4 cut(s) 10, 25, 439, 761
BtsCI GGATG 2 cut(s) 142, 298
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 3 cut(s) 231, 341, 799
Cac8I GCNNGC 1 cut(s) 606
CaiI CAGNNNCTG 1 cut(s) 855
CciI TCATGA 1 cut(s) 550
Cfr13I GGNCC 2 cut(s) 24, 437
CseI GACGC 1 cut(s) 740
CviAII CATG 6 cut(s) 14, 274, 458, 551, 575, 605
DdeI CTNAG 3 cut(s) 38, 180, 702
DpnI GATC 4 cut(s) 263, 423, 600, 639
DpnII GATC 4 cut(s) 261, 421, 598, 637
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 5
Eco147I AGGCCT 2 cut(s) 10, 761
Eco24I GRGCYC 2 cut(s) 47, 170
Eco53kI GAGCTC 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 168
EcoNI CCTNNNNNAGG 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 426, 714
EcoT14I CCWWGG 1 cut(s) 5
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 2 cut(s) 47, 170
ErhI CCWWGG 1 cut(s) 5
FaeI CATG 6 cut(s) 17, 277, 461, 554, 578, 608
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 1 cut(s) 89
FatI CATG 6 cut(s) 13, 273, 457, 550, 574, 604
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 686, 820
FokI GGATG 2 cut(s) 149, 285
FriOI GRGCYC 2 cut(s) 47, 170
Fsp4HI GCNGC 2 cut(s) 686, 820
FspBI CTAG 5 cut(s) 6, 129, 171, 249, 285
GluI GCNGC 2 cut(s) 686, 820
HaeIII GGCC 4 cut(s) 10, 25, 439, 761
HapII CCGG 3 cut(s) 70, 347, 742
HgaI GACGC 1 cut(s) 740
Hin1I GRCGYC 1 cut(s) 845
Hin1II CATG 6 cut(s) 17, 277, 461, 554, 578, 608
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 4 cut(s) 513, 751, 770, 852
HpaII CCGG 3 cut(s) 70, 347, 742
HphI GGTGA 4 cut(s) 442, 458, 523, 534
Hpy166II GTNNAC 3 cut(s) 361, 727, 808
Hpy188I TCNGA 3 cut(s) 642, 705, 857
Hpy188III TCNNGA 6 cut(s) 171, 179, 410, 467, 551, 849
Hpy8I GTNNAC 3 cut(s) 361, 727, 808
Hpy99I CGWCG 1 cut(s) 734
HpyAV CCTTC 3 cut(s) 427, 450, 821
HpyCH4III ACNGT 3 cut(s) 257, 358, 416
HpyCH4IV ACGT 1 cut(s) 845
HpyCH4V TGCA 3 cut(s) 339, 392, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 691
HpyF3I CTNAG 3 cut(s) 38, 180, 702
HpySE526I ACGT 1 cut(s) 845
Hsp92I GRCGYC 1 cut(s) 845
Hsp92II CATG 6 cut(s) 17, 277, 461, 554, 578, 608
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 261, 421, 598, 637
LmnI GCTCC 1 cut(s) 42
Lsp1109I GCAGC 1 cut(s) 697
LweI GCATC 2 cut(s) 379, 401
MaeI CTAG 5 cut(s) 6, 129, 171, 249, 285
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 2 cut(s) 496, 540
MalI GATC 4 cut(s) 263, 423, 600, 639
MboI GATC 4 cut(s) 261, 421, 598, 637
MboII GAAGA 2 cut(s) 334, 806
MhlI GDGCHC 2 cut(s) 47, 170
MluCI AATT 3 cut(s) 156, 378, 485
MmeI TCCRAC 1 cut(s) 77
Mph1103I ATGCAT 1 cut(s) 394
MseI TTAA 2 cut(s) 381, 624
MslI CAYNNNNRTG 1 cut(s) 80
MspI CCGG 3 cut(s) 70, 347, 742
MspR9I CCNGG 3 cut(s) 348, 428, 716
MvaI CCWGG 2 cut(s) 428, 716
MwoI GCNNNNNNNGC 1 cut(s) 691
NciI CCSGG 1 cut(s) 348
NdeII GATC 4 cut(s) 261, 421, 598, 637
NlaIII CATG 6 cut(s) 17, 277, 461, 554, 578, 608
NlaIV GGNNCC 4 cut(s) 44, 345, 438, 790
NmuCI GTSAC 2 cut(s) 496, 540
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 2 cut(s) 578, 608
PaeI GCATGC 1 cut(s) 608
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 2 cut(s) 10, 761
PciI ACATGT 1 cut(s) 574
PfeI GAWTC 3 cut(s) 513, 751, 770
PkrI GCNGC 2 cut(s) 687, 821
PscI ACATGT 1 cut(s) 574
Psp124BI GAGCTC 1 cut(s) 170
Psp6I CCWGG 2 cut(s) 426, 714
PspGI CCWGG 2 cut(s) 426, 714
PspN4I GGNNCC 4 cut(s) 44, 345, 438, 790
PspPI GGNCC 2 cut(s) 24, 437
PstNI CAGNNNCTG 1 cut(s) 855
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 381, 624
SatI GCNGC 2 cut(s) 686, 820
Sau3AI GATC 4 cut(s) 261, 421, 598, 637
Sau96I GGNCC 2 cut(s) 24, 437
ScrFI CCNGG 3 cut(s) 348, 428, 716
SduI GDGCHC 2 cut(s) 47, 170
SfaNI GCATC 2 cut(s) 379, 401
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 2 cut(s) 408, 695
SmoI CTYRAG 2 cut(s) 408, 695
SphI GCATGC 1 cut(s) 608
Sse9I AATT 3 cut(s) 156, 378, 485
SseBI AGGCCT 2 cut(s) 10, 761
SsiI CCGC 2 cut(s) 302, 820
SspMI CTAG 5 cut(s) 6, 129, 171, 249, 285
SstI GAGCTC 1 cut(s) 170
StuI AGGCCT 2 cut(s) 10, 761
StyD4I CCNGG 3 cut(s) 346, 426, 714
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 3 cut(s) 257, 358, 416
TaiI ACGT 1 cut(s) 848
TaqI TCGA 1 cut(s) 489
TasI AATT 3 cut(s) 156, 378, 485
TauI GCSGC 1 cut(s) 822
TfiI GAWTC 3 cut(s) 513, 751, 770
Tru1I TTAA 2 cut(s) 381, 624
Tru9I TTAA 2 cut(s) 381, 624
TscAI CASTG 3 cut(s) 231, 341, 799
TseFI GTSAC 2 cut(s) 496, 540
TseI GCWGC 1 cut(s) 685
Tsp45I GTSAC 2 cut(s) 496, 540
TspDTI ATGAA 1 cut(s) 567
TspGWI ACGGA 1 cut(s) 847
TspRI CASTG 3 cut(s) 231, 341, 799
XagI CCTNNNNNAGG 1 cut(s) 431
XapI RAATTY 1 cut(s) 485
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 2 cut(s) 578, 608
XmaJI CCTAGG 1 cut(s) 5
XspI CTAG 5 cut(s) 6, 129, 171, 249, 285
ZraI GACGTC 1 cut(s) 846
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.