Rmu_sc0004688.1_g000028

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004688.1
N/A
Physical Location & Seq
Forward (+)
83515 .. 90773
7259 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1590 bp
atgttgagaagaagccgtttcgcgattctgatctcaactctgctgcttctcagctccgatttgcttctctcactctcgctcgcagccgccgaccctctcgccgacgactcagatcccctcctcctcaacctcaacgccaccgagtccaatgtctcgctctccaagcccaaggaaggcagcttcgctgacatgatcgacaaggccctcgagaacgagttcaaggagaacgatcagaatcaagctactgatcctgggagttttaacaacagcgtcgccggccaacaggcaacactggaaacagttgccagagttaagtcgaagaaaaatgagacaaaagaggaaaacttaaataggtcgtttcaactgcatgatgtttttaatctggataatgataatggagcagaagagaccccaacactcatagatcgcaaggacaatgtctttataatatcaaactttaaatcaaaatatccagtgctgcagctagacctgagactgatatcagatctcgtcgtggttattgtatctgcaacctgtggtggcattgcttttgcttgtgctggacaaccggttatctctggatatttgctagctggatctgttattggacctggagggttcaacttcgtcagtgaaatggttcaagttgaaactgtagctcaatttggggtgatctttcttctttttgctttgggcttagagttctccacaacaaagcttcgagttgttcgagcagttgctgttccgggaggcctgctgcaaattttcctatttatgtgcctgtgtggtattacagcctcattatgtggtggtaaaatttctgagggggtattcgttggcgtgttcctttctatgtcttcaacagcagtgtgtagtgacaagctgggattaagtctggaactgggttcctttgccgcaggagttatgatatcaacaactgatcttgctcaacatacactagaacaaattgaacccattcgcaacctttttgctgcccttttccttgccagcattgggatgctgattcatgttcagtttctctggaatcatgttgatatattactcgcgtctgtcatcttagttatcatcataaaaacaattattataaccatggttgtcaagggattcggatacaacaacaaaacatcattcctagttggaatatctctagcacagataggcgaatttgcttttgttcttttaagtcgtgcctctaatctccatctagtcgaggggaaattatatctgctgctgcttggaaccacagcacttagtctggtgaccactccttttctattcaagctaatccctggtttagtgcatcttggtgtacttttgaggtggttccctccagacggtgctgttgagattggatttaagggagacaacctgcgcacggatagtgcgattggaggtggctatgtgggtcacagcgattggagccttggaggtggctccgtggctggagcctggaagctggaactttggaagagtggaaggcggaaccgatatgaagttttgaacgaggagacggggttggtgtttgagagtcgggaaagactggtcttgggaccgactgaccgacaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

529

Amino Acids

57.11

Weight (kDa)

5.33

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 174 - 290 5e-23 Sodium/hydrogen exchanger, transmembrane
Na_H_Exchanger PF00999 288 - 434 4.2e-29 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 446, 1106
Acc16I TGCGCA 1 cut(s) 1394
Acc36I ACCTGC 1 cut(s) 1398
AccII CGCG 2 cut(s) 23, 1067
AciI CCGC 3 cut(s) 87, 915, 1501
AclWI GGATC 3 cut(s) 107, 242, 604
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 3 cut(s) 762, 816, 1184
AfaI GTAC 1 cut(s) 1332
AfiI CCNNNNNNNGG 4 cut(s) 173, 1014, 1355, 1396
AgeI ACCGGT 1 cut(s) 568
AgsI TTSAA 9 cut(s) 220, 362, 622, 644, 650, 861, 971, 1300, 1522
AjnI CCWGG 4 cut(s) 250, 610, 1309, 1469
Alw26I GTCTC 6 cut(s) 157, 323, 401, 487, 1377, 1523
AlwI GGATC 3 cut(s) 107, 242, 604
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 3 cut(s) 201, 277, 751
ApeKI GCWGC 9 cut(s) 43, 83, 177, 478, 481, 757, 992, 1249, 1252
ApoI RAATTY 3 cut(s) 762, 816, 1184
AsiGI ACCGGT 1 cut(s) 568
Asp700I GAANNNNTTC 3 cut(s) 215, 639, 975
AspLEI GCGC 1 cut(s) 1395
AspS9I GGNCC 3 cut(s) 202, 608, 1571
AsuC2I CCSGG 1 cut(s) 747
AsuHPI GGTGA 2 cut(s) 682, 1291
AsuNHI GCTAGC 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 2 cut(s) 608, 1571
BbsI GAAGAC 1 cut(s) 849
BbvI GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 979, 1236, 1239
BccI CCATC 1 cut(s) 1230
BciT130I CCWGG 4 cut(s) 252, 612, 1311, 1471
BciVI GTATCC 1 cut(s) 1124
BcnI CCSGG 1 cut(s) 747
BcoDI GTCTC 6 cut(s) 157, 323, 401, 487, 1377, 1523
BfaI CTAG 6 cut(s) 485, 590, 959, 1154, 1169, 1226
BfmI CTRYAG 2 cut(s) 479, 654
BfuAI ACCTGC 1 cut(s) 1398
BfuI GTATCC 1 cut(s) 1124
BglII AGATCT 1 cut(s) 505
Bme1390I CCNGG 5 cut(s) 252, 612, 747, 1311, 1471
Bme18I GGWCC 2 cut(s) 608, 1571
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 3 cut(s) 202, 608, 1571
BmiI GGNNCC 8 cut(s) 907, 1261, 1346, 1442, 1456, 1468, 1505, 1572
BmrFI CCNGG 5 cut(s) 252, 612, 747, 1311, 1471
BmrI ACTGGG 1 cut(s) 911
BmsI GCATC 2 cut(s) 1008, 1330
BmtI GCTAGC 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 911
BpiI GAAGAC 1 cut(s) 849
BpmI CTGGAG 3 cut(s) 633, 1335, 1485
BpuMI CCSGG 1 cut(s) 747
BsaBI GATNNNNATC 2 cut(s) 29, 234
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 6 cut(s) 168, 251, 1110, 1309, 1444, 1458
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 2 cut(s) 1520, 1550
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 1014, 1355, 1396
Bse118I RCCGGY 2 cut(s) 275, 568
Bse1I ACTGG 4 cut(s) 297, 473, 906, 1566
Bse3DI GCAATG 1 cut(s) 543
Bse8I GATNNNNATC 2 cut(s) 29, 234
BseBI CCWGG 4 cut(s) 252, 612, 1311, 1471
BseDI CCNNGG 6 cut(s) 168, 251, 1110, 1309, 1444, 1458
BseGI GGATG 1 cut(s) 1023
BseJI GATNNNNATC 2 cut(s) 29, 234
BseLI CCNNNNNNNGG 4 cut(s) 173, 1014, 1355, 1396
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 4 cut(s) 64, 123, 482, 813
BseNI ACTGG 4 cut(s) 297, 473, 906, 1566
BseRI GAGGAG 3 cut(s) 110, 113, 1541
BseXI GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 979, 1236, 1239
BseYI CCCAGC 1 cut(s) 883
Bsh1236I CGCG 2 cut(s) 23, 1067
BshFI GGCC 3 cut(s) 203, 279, 753
BshTI ACCGGT 1 cut(s) 568
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 276, 569, 746
BslFI GGGAC 1 cut(s) 1584
BslI CCNNNNNNNGG 4 cut(s) 173, 1014, 1355, 1396
BsmAI GTCTC 6 cut(s) 157, 323, 401, 487, 1377, 1523
BsmBI CGTCTC 1 cut(s) 1523
BsmFI GGGAC 1 cut(s) 1584
BsnI GGCC 3 cut(s) 203, 279, 753
Bso31I GGTCTC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 206
Bsp19I CCATGG 1 cut(s) 1110
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 3 cut(s) 87, 915, 1501
BspANI GGCC 3 cut(s) 203, 279, 753
BspCNI CTCAG 4 cut(s) 63, 122, 483, 814
BspFNI CGCG 2 cut(s) 23, 1067
BspLI GGNNCC 8 cut(s) 907, 1261, 1346, 1442, 1456, 1468, 1505, 1572
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 1398
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 3 cut(s) 107, 242, 604
BspTNI GGTCTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 2 cut(s) 275, 568
BsrI ACTGG 4 cut(s) 297, 473, 906, 1566
BssAI RCCGGY 2 cut(s) 275, 568
BssECI CCNNGG 6 cut(s) 168, 251, 1110, 1309, 1444, 1458
BssT1I CCWWGG 3 cut(s) 168, 1110, 1444
Bst2UI CCWGG 4 cut(s) 252, 612, 1311, 1471
Bst4CI ACNGT 3 cut(s) 301, 655, 1358
Bst6I CTCTTC 2 cut(s) 399, 1484
BstC8I GCNNGC 5 cut(s) 81, 277, 591, 755, 1009
BstDEI CTNAG 7 cut(s) 50, 109, 491, 697, 822, 1078, 1271
BstDSI CCRYGG 2 cut(s) 1110, 1458
BstEII GGTNACC 1 cut(s) 1279
BstF5I GGATG 1 cut(s) 1023
BstFNI CGCG 2 cut(s) 23, 1067
BstHHI GCGC 1 cut(s) 1395
BstMAI GTCTC 6 cut(s) 157, 323, 401, 487, 1377, 1523
BstMWI GCNNNNNNNGC 3 cut(s) 163, 276, 1440
BstNI CCWGG 4 cut(s) 252, 612, 1311, 1471
BstPI GGTNACC 1 cut(s) 1279
BstSCI CCNGG 5 cut(s) 250, 610, 745, 1309, 1469
BstSFI CTRYAG 2 cut(s) 479, 654
BstUI CGCG 2 cut(s) 23, 1067
BstV1I GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 979, 1236, 1239
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 3 cut(s) 112, 505, 596
BstYI RGATCY 3 cut(s) 112, 505, 596
BsuI GTATCC 1 cut(s) 1124
BsuRI GGCC 3 cut(s) 203, 279, 753
BtgI CCRYGG 2 cut(s) 1110, 1458
BtsCI GGATG 1 cut(s) 1023
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 4 cut(s) 290, 480, 637, 873
BtuMI TCGCGA 1 cut(s) 23
BveI ACCTGC 1 cut(s) 1398
Cac8I GCNNGC 5 cut(s) 81, 277, 591, 755, 1009
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 1 cut(s) 1395
Cfr10I RCCGGY 2 cut(s) 275, 568
Cfr13I GGNCC 3 cut(s) 202, 608, 1571
CseI GACGC 2 cut(s) 259, 1056
Csp6I GTAC 1 cut(s) 1331
CspAI ACCGGT 1 cut(s) 568
CviAII CATG 5 cut(s) 190, 368, 1028, 1049, 1111
CviQI GTAC 1 cut(s) 1331
DdeI CTNAG 7 cut(s) 50, 109, 491, 697, 822, 1078, 1271
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 399, 1484
EarI CTCTTC 2 cut(s) 399, 1484
EciI GGCGGA 1 cut(s) 1516
Eco130I CCWWGG 3 cut(s) 168, 1110, 1444
Eco147I AGGCCT 1 cut(s) 753
Eco31I GGTCTC 1 cut(s) 401
Eco32I GATATC 2 cut(s) 501, 930
Eco47I GGWCC 2 cut(s) 608, 1571
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 1279
EcoO109I RGGNCCY 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 1279
EcoRII CCWGG 4 cut(s) 250, 610, 1309, 1469
EcoRV GATATC 2 cut(s) 501, 930
EcoT14I CCWWGG 3 cut(s) 168, 1110, 1444
ErhI CCWWGG 3 cut(s) 168, 1110, 1444
Esp3I CGTCTC 1 cut(s) 1523
FaeI CATG 5 cut(s) 193, 371, 1031, 1052, 1114
FaqI GGGAC 1 cut(s) 1584
FatI CATG 5 cut(s) 189, 367, 1027, 1048, 1110
FokI GGATG 1 cut(s) 1030
FspBI CTAG 6 cut(s) 485, 590, 959, 1154, 1169, 1226
FspI TGCGCA 1 cut(s) 1394
GlaI GCGC 1 cut(s) 1394
GsaI CCCAGC 1 cut(s) 887
GsuI CTGGAG 3 cut(s) 633, 1335, 1485
HaeIII GGCC 3 cut(s) 203, 279, 753
HapII CCGG 3 cut(s) 276, 569, 746
HgaI GACGC 2 cut(s) 259, 1056
HhaI GCGC 1 cut(s) 1395
Hin1II CATG 5 cut(s) 193, 371, 1031, 1052, 1114
Hin6I GCGC 1 cut(s) 1393
HinP1I GCGC 1 cut(s) 1393
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 8 cut(s) 25, 107, 143, 235, 1024, 1045, 1125, 1549
HpaII CCGG 3 cut(s) 276, 569, 746
HphI GGTGA 2 cut(s) 682, 1291
Hpy166II GTNNAC 1 cut(s) 1331
Hpy188I TCNGA 7 cut(s) 30, 58, 112, 234, 505, 823, 1130
Hpy188III TCNNGA 8 cut(s) 22, 208, 383, 579, 896, 1042, 1352, 1553
Hpy8I GTNNAC 1 cut(s) 1331
Hpy99I CGWCG 3 cut(s) 107, 275, 515
HpyAV CCTTC 2 cut(s) 167, 1491
HpyCH4III ACNGT 3 cut(s) 301, 655, 1358
HpyCH4V TGCA 5 cut(s) 367, 481, 530, 760, 1321
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 276, 1440
HpyF3I CTNAG 7 cut(s) 50, 109, 491, 697, 822, 1078, 1271
Hsp92II CATG 5 cut(s) 193, 371, 1031, 1052, 1114
HspAI GCGC 1 cut(s) 1393
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
LmnI GCTCC 5 cut(s) 59, 398, 1440, 1460, 1466
Lsp1109I GCAGC 9 cut(s) 30, 95, 189, 465, 493, 744, 979, 1236, 1239
LweI GCATC 2 cut(s) 1008, 1330
MaeI CTAG 6 cut(s) 485, 590, 959, 1154, 1169, 1226
MaeIII GTNAC 3 cut(s) 875, 1279, 1427
MboII GAAGA 6 cut(s) 21, 331, 416, 671, 849, 1501
MflI RGATCY 3 cut(s) 112, 505, 596
MluCI AATT 7 cut(s) 662, 762, 816, 966, 1098, 1184, 1238
MlyI GAGTC 3 cut(s) 101, 152, 1558
MmeI TCCRAC 1 cut(s) 1138
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 3 cut(s) 215, 639, 975
MseI TTAA 8 cut(s) 261, 312, 347, 378, 459, 890, 1202, 1377
MslI CAYNNNNRTG 2 cut(s) 1016, 1326
MspI CCGG 3 cut(s) 276, 569, 746
MspR9I CCNGG 5 cut(s) 252, 612, 747, 1311, 1471
MvaI CCWGG 4 cut(s) 252, 612, 1311, 1471
MvnI CGCG 2 cut(s) 23, 1067
MwoI GCNNNNNNNGC 3 cut(s) 163, 276, 1440
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 747
NcoI CCATGG 1 cut(s) 1110
NgoMIV GCCGGC 1 cut(s) 275
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 5 cut(s) 193, 371, 1031, 1052, 1114
NlaIV GGNNCC 8 cut(s) 907, 1261, 1346, 1442, 1456, 1468, 1505, 1572
NmuCI GTSAC 3 cut(s) 875, 1279, 1427
NruI TCGCGA 1 cut(s) 23
NsbI TGCGCA 1 cut(s) 1394
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 727, 1403
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 3 cut(s) 215, 639, 975
PfeI GAWTC 5 cut(s) 25, 235, 1024, 1045, 1125
PflFI GACNNNGTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 745
PinAI ACCGGT 1 cut(s) 568
PleI GAGTC 3 cut(s) 101, 151, 1557
PpsI GAGTC 3 cut(s) 101, 151, 1557
PsiI TTATAA 2 cut(s) 446, 1106
Psp6I CCWGG 4 cut(s) 250, 610, 1309, 1469
PspEI GGTNACC 1 cut(s) 1279
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 4 cut(s) 250, 610, 1309, 1469
PspN4I GGNNCC 8 cut(s) 907, 1261, 1346, 1442, 1456, 1468, 1505, 1572
PspPI GGNCC 3 cut(s) 202, 608, 1571
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 3 cut(s) 112, 505, 596
PsyI GACNNNGTC 1 cut(s) 437
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 1 cut(s) 1332
RsaNI GTAC 1 cut(s) 1331
RseI CAYNNNNRTG 2 cut(s) 1016, 1326
SaqAI TTAA 8 cut(s) 261, 312, 347, 378, 459, 890, 1202, 1377
Sau96I GGNCC 3 cut(s) 202, 608, 1571
SchI GAGTC 3 cut(s) 101, 152, 1558
ScrFI CCNGG 5 cut(s) 252, 612, 747, 1311, 1471
SfaNI GCATC 2 cut(s) 1008, 1330
SfcI CTRYAG 2 cut(s) 479, 654
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 608, 1571
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 1016, 1326
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 7 cut(s) 662, 762, 816, 966, 1098, 1184, 1238
SseBI AGGCCT 1 cut(s) 753
SsiI CCGC 3 cut(s) 87, 915, 1501
SspMI CTAG 6 cut(s) 485, 590, 959, 1154, 1169, 1226
StuI AGGCCT 1 cut(s) 753
StyD4I CCNGG 5 cut(s) 250, 610, 745, 1309, 1469
StyI CCWWGG 3 cut(s) 168, 1110, 1444
TaaI ACNGT 3 cut(s) 301, 655, 1358
TaqI TCGA 6 cut(s) 195, 207, 317, 721, 730, 1230
TaqII GACCGA 1 cut(s) 1588
TasI AATT 7 cut(s) 662, 762, 816, 966, 1098, 1184, 1238
TatI WGTACW 1 cut(s) 1330
TauI GCSGC 2 cut(s) 89, 917
TfiI GAWTC 5 cut(s) 25, 235, 1024, 1045, 1125
Tru1I TTAA 8 cut(s) 261, 312, 347, 378, 459, 890, 1202, 1377
Tru9I TTAA 8 cut(s) 261, 312, 347, 378, 459, 890, 1202, 1377
TscAI CASTG 4 cut(s) 297, 480, 637, 873
TseFI GTSAC 3 cut(s) 875, 1279, 1427
TseI GCWGC 9 cut(s) 43, 83, 177, 478, 481, 757, 992, 1249, 1252
Tsp45I GTSAC 3 cut(s) 875, 1279, 1427
TspDTI ATGAA 2 cut(s) 1016, 1527
TspGWI ACGGA 2 cut(s) 1412, 1447
TspRI CASTG 4 cut(s) 297, 480, 637, 873
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 608, 1571
XapI RAATTY 3 cut(s) 762, 816, 1184
XhoI CTCGAG 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 215, 639, 975
XspI CTAG 6 cut(s) 485, 590, 959, 1154, 1169, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.