Rmu_sc0004855.1_g000015

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004855.1
N/A
Physical Location & Seq
Reverse (-)
61359 .. 63647
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
atgcagggcggctttgacatgattgctagtggcagagacaagattgaaactccagaacagtttcagcaagctgcagatacagcgaacaagcttgatttagatgggcttgtggtcattggtggggatgactcaaatactaatgcttgccttcttgctgagaacttcaggggtaataatttgaagacacgggttattggatgtccaaaaaccattgatggtgatctgaaatgcaaagaggtgcctacaagttttggatttgacacagcatgcagggttgctgccaagaagcagactctcaagaatgtcacagattacataacagatattgtctgcaaaagtgcagaacagggttataattatggtattgtacttataccggaaggcctgattgatttcattccagaggtacagcaactaattgcagagcttaatgaaatcttagctcatggtgctgttgatgaagcgggggagtggaaaaagaaacttcagcctcaatctcatgagctttttgagtttctaccacgagcaattcaagagcaactattgcttgaaagagatccacatggcaatgtgcaggtaaattatattttttctcatgtacgtagttcgactttttga

Protein Analysis

205

Amino Acids

22.7

Weight (kDa)

4.86

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 7 - 95 1e-14 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 238
AciI CCGC 2 cut(s) 9, 464
AclWI GGATC 1 cut(s) 551
AcuI CTGAAG 2 cut(s) 148, 470
AfaI GTAC 3 cut(s) 369, 408, 600
AgsI TTSAA 4 cut(s) 47, 181, 533, 551
AluBI AGCT 5 cut(s) 71, 91, 427, 443, 505
AluI AGCT 5 cut(s) 71, 91, 427, 443, 505
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 1 cut(s) 551
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 2 cut(s) 71, 278
Asp700I GAANNNNTTC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 230
BanI GGYRCC 1 cut(s) 238
BauI CACGAG 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 58, 265
BccI CCATC 2 cut(s) 95, 209
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 3 cut(s) 10, 72, 279
BlsI GCNGC 3 cut(s) 11, 73, 280
BmiI GGNNCC 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 602
BsaBI GATNNNNATC 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 376
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 219
BseGI GGATG 2 cut(s) 130, 203
BseJI GATNNNNATC 1 cut(s) 219
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 58, 265
BsgI GTGCAG 2 cut(s) 360, 593
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 30
BsnI GGCC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 220, 556
BspACI CCGC 2 cut(s) 9, 464
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 1 cut(s) 551
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 574
BssMI GATC 2 cut(s) 220, 556
BssSI CACGAG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 544
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 69, 145, 268
BstDEI CTNAG 2 cut(s) 156, 439
BstF5I GGATG 2 cut(s) 130, 203
BstKTI GATC 2 cut(s) 223, 559
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 2 cut(s) 220, 556
BstMWI GCNNNNNNNGC 3 cut(s) 80, 449, 544
BstNSI RCATGY 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 72
BstSNI TACGTA 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 58, 265
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 2 cut(s) 130, 203
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 3 cut(s) 69, 145, 268
CciI TCATGA 1 cut(s) 499
Csp6I GTAC 3 cut(s) 368, 407, 599
CviAII CATG 6 cut(s) 19, 267, 446, 500, 563, 596
CviJI RGCY 9 cut(s) 12, 71, 91, 106, 384, 427, 443, 490, 505
CviKI_1 RGCY 9 cut(s) 12, 71, 91, 106, 384, 427, 443, 490, 505
CviQI GTAC 3 cut(s) 368, 407, 599
DdeI CTNAG 2 cut(s) 156, 439
DpnI GATC 2 cut(s) 222, 558
DpnII GATC 2 cut(s) 220, 556
Eco105I TACGTA 1 cut(s) 602
Eco147I AGGCCT 1 cut(s) 384
Eco57I CTGAAG 2 cut(s) 148, 470
FaeI CATG 6 cut(s) 22, 270, 449, 503, 566, 599
FatI CATG 6 cut(s) 18, 266, 445, 499, 562, 595
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 3 cut(s) 10, 72, 279
FokI GGATG 2 cut(s) 137, 210
Fsp4HI GCNGC 3 cut(s) 10, 72, 279
FspBI CTAG 1 cut(s) 27
GluI GCNGC 3 cut(s) 10, 72, 279
GsuI CTGGAG 1 cut(s) 36
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 377
Hin1II CATG 6 cut(s) 22, 270, 449, 503, 566, 599
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 128, 292
HpaII CCGG 1 cut(s) 377
HphI GGTGA 1 cut(s) 230
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 5 cut(s) 53, 298, 401, 500, 533
HpyAV CCTTC 2 cut(s) 158, 374
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 8 cut(s) 4, 74, 231, 270, 333, 341, 422, 574
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 449, 544
HpyF3I CTNAG 2 cut(s) 156, 439
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 6 cut(s) 22, 270, 449, 503, 566, 599
Kzo9I GATC 2 cut(s) 220, 556
LpnPI CCDG 8 cut(s) 66, 151, 256, 332, 390, 398, 414, 560
Lsp1109I GCAGC 2 cut(s) 58, 265
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 222, 558
MboI GATC 2 cut(s) 220, 556
MboII GAAGA 1 cut(s) 193
MflI RGATCY 1 cut(s) 556
MluCI AATT 5 cut(s) 175, 355, 417, 528, 580
MlyI GAGTC 2 cut(s) 122, 286
MnlI CCTC 3 cut(s) 229, 397, 501
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 80, 449, 544
NdeII GATC 2 cut(s) 220, 556
NlaIII CATG 6 cut(s) 22, 270, 449, 503, 566, 599
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 1 cut(s) 270
PaeI GCATGC 1 cut(s) 270
PagI TCATGA 1 cut(s) 499
PceI AGGCCT 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 60
PkrI GCNGC 3 cut(s) 11, 73, 280
PleI GAGTC 2 cut(s) 122, 286
PpsI GAGTC 2 cut(s) 122, 286
Ppu21I YACGTR 1 cut(s) 602
PsiI TTATAA 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 240
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 3 cut(s) 369, 408, 600
RsaNI GTAC 3 cut(s) 368, 407, 599
RseI CAYNNNNRTG 1 cut(s) 567
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 3 cut(s) 10, 72, 279
Sau3AI GATC 2 cut(s) 220, 556
SchI GAGTC 2 cut(s) 122, 286
SetI ASST 9 cut(s) 73, 93, 240, 408, 429, 445, 507, 579, 604
SfcI CTRYAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 602
SphI GCATGC 1 cut(s) 270
Sse9I AATT 5 cut(s) 175, 355, 417, 528, 580
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 2 cut(s) 9, 464
SspMI CTAG 1 cut(s) 27
StuI AGGCCT 1 cut(s) 384
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 608
TasI AATT 5 cut(s) 175, 355, 417, 528, 580
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 2 cut(s) 71, 278
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 385, 447, 474
XceI RCATGY 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.