Rmu_sc0004884.1_g000001

S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004884.1
N/A
Physical Location & Seq
Reverse (-)
2 .. 888
887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
atgttcgtggtttcaaagattttctttaatggagcagtaagcagatccaacatattgggccgcgaacaagtagccgaaaacgataagaagtatcatgacctgaactcggctcttcttcgtcgtctgaaagggcaaaaccggaaattcgatttcccgcccattatgcccacatcctcagcccgagcacggctggctctcatagccctcctctccgccaccacgttctactgtttctacaaatctcgccgcctcaagcaactctccctaaaccctaaccctagctcctctccgcctcgcataggcaagctcttcttcatttcccaaaccggaacctccaaagccctagctcagcgtcttctaaaacttctagcctccaatggcctcgccttcgagctcgtcgaccctagcagctacgagccagaggacctccccaaggaaaccctagtcctgatcgtcgcttccacctgggacgatggacaaccgccttcgggcgccaagttcttcaccaattggctcgcggagagcgccgaggacttcagagtggggtccatgctgctctcgcagtgcagattctcggtgttcggcgtcggaagccgagcctacggtgcgtcgttcaatgcggtggggagggacttgtcgaagtggatgagggccttgggcgccgccgagatattgccggtttgggaaggtgacgtggacgacggggatatagaccaggtctttgaggcttggagtggtaagctgatgaggtttttgaaggccggtgtggccgaaaacggtgtcgctttgggtaatggtgtt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Flavodoxin_1 Radical_SAM Wyosine_form

Protein Analysis

267

Amino Acids

29.34

Weight (kDa)

9.34

Isoelectric Point (pI)

33.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 103 - 244 1.6e-27 Flavodoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 491, 659
AccI GTMKAC 1 cut(s) 399
AccII CGCG 2 cut(s) 63, 518
AciI CCGC 9 cut(s) 61, 155, 213, 247, 290, 482, 518, 620, 663
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 768
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 520
AcyI GRCGYC 3 cut(s) 492, 585, 660
AfiI CCNNNNNNNGG 5 cut(s) 106, 186, 299, 433, 488
AgsI TTSAA 3 cut(s) 15, 616, 757
AjiI CACGTC 1 cut(s) 694
AjnI CCWGG 2 cut(s) 464, 714
AluBI AGCT 6 cut(s) 282, 307, 347, 394, 411, 742
AluI AGCT 6 cut(s) 282, 307, 347, 394, 411, 742
Alw21I GWGCWC 2 cut(s) 187, 396
AlwI GGATC 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 5 cut(s) 58, 379, 651, 759, 768
ApeKI GCWGC 2 cut(s) 408, 553
ApoI RAATTY 1 cut(s) 143
ArsI GACNNNNNNTTYG 4 cut(s) 704, 736, 765, 797
AspLEI GCGC 3 cut(s) 494, 527, 662
AspS9I GGNCC 4 cut(s) 58, 424, 546, 651
AsuHPI GGTGA 2 cut(s) 496, 701
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 2 cut(s) 424, 546
BanI GGYRCC 2 cut(s) 491, 659
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 2 cut(s) 187, 396
BbvCI CCTCAGC 1 cut(s) 175
BbvI GCAGC 2 cut(s) 420, 540
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 466, 716
BfaI CTAG 5 cut(s) 279, 344, 368, 405, 443
BfoI RGCGCY 3 cut(s) 495, 528, 663
BglI GCCNNNNNGGC 1 cut(s) 767
BisI GCNGC 5 cut(s) 61, 247, 409, 554, 663
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 5 cut(s) 62, 248, 410, 555, 664
Bme1390I CCNGG 2 cut(s) 466, 716
Bme18I GGWCC 2 cut(s) 424, 546
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 4 cut(s) 58, 424, 546, 651
BmiI GGNNCC 4 cut(s) 331, 493, 547, 661
BmrFI CCNGG 2 cut(s) 466, 716
BpiI GAAGAC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 175
Bpu1102I GCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 236
BsaHI GRCGYC 3 cut(s) 492, 585, 660
BsaJI CCNNGG 4 cut(s) 432, 465, 528, 654
BsaWI WCCGGW 2 cut(s) 138, 326
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 186, 299, 433, 488
Bse118I RCCGGY 2 cut(s) 676, 761
BseBI CCWGG 2 cut(s) 466, 716
BseDI CCNNGG 4 cut(s) 432, 465, 528, 654
BseGI GGATG 2 cut(s) 170, 651
BseLI CCNNNNNNNGG 5 cut(s) 106, 186, 299, 433, 488
BseMII CTCAG 2 cut(s) 189, 362
BseRI GAGGAG 2 cut(s) 197, 274
BseXI GCAGC 2 cut(s) 420, 540
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 2 cut(s) 63, 518
BshFI GGCC 5 cut(s) 60, 381, 653, 761, 770
BshNI GGYRCC 2 cut(s) 491, 659
BsiHKAI GWGCWC 2 cut(s) 187, 396
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 4 cut(s) 139, 327, 677, 762
BslFI GGGAC 2 cut(s) 482, 644
BslI CCNNNNNNNGG 5 cut(s) 106, 186, 299, 433, 488
BsmFI GGGAC 2 cut(s) 482, 644
BsnI GGCC 5 cut(s) 60, 381, 653, 761, 770
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 187, 396
Bsp143I GATC 2 cut(s) 44, 450
Bsp1720I GCTNAGC 1 cut(s) 348
BspACI CCGC 9 cut(s) 61, 155, 213, 247, 290, 482, 518, 620, 663
BspANI GGCC 5 cut(s) 60, 381, 653, 761, 770
BspCNI CTCAG 2 cut(s) 188, 361
BspFNI CGCG 2 cut(s) 63, 518
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 4 cut(s) 331, 493, 547, 661
BspPI GGATC 1 cut(s) 39
BspQI GCTCTTC 2 cut(s) 117, 314
BspT107I GGYRCC 2 cut(s) 491, 659
BsrFI RCCGGY 2 cut(s) 676, 761
BssAI RCCGGY 2 cut(s) 676, 761
BssECI CCNNGG 4 cut(s) 432, 465, 528, 654
BssMI GATC 2 cut(s) 44, 450
BssNI GRCGYC 3 cut(s) 492, 585, 660
BssT1I CCWWGG 2 cut(s) 432, 654
Bst2UI CCWGG 2 cut(s) 466, 716
Bst4CI ACNGT 3 cut(s) 230, 605, 779
Bst6I CTCTTC 2 cut(s) 117, 314
BstACI GRCGYC 3 cut(s) 492, 585, 660
BstC8I GCNNGC 3 cut(s) 192, 305, 516
BstDEI CTNAG 2 cut(s) 175, 348
BstENI CCTNNNNNAGG 2 cut(s) 297, 431
BstF5I GGATG 2 cut(s) 170, 651
BstFNI CGCG 2 cut(s) 63, 518
BstH2I RGCGCY 3 cut(s) 495, 528, 663
BstHHI GCGC 3 cut(s) 494, 527, 662
BstKTI GATC 2 cut(s) 47, 453
BstMBI GATC 2 cut(s) 44, 450
BstMWI GCNNNNNNNGC 9 cut(s) 163, 191, 200, 524, 559, 591, 605, 659, 767
BstNI CCWGG 2 cut(s) 466, 716
BstSCI CCNGG 2 cut(s) 464, 714
BstUI CGCG 2 cut(s) 63, 518
BstV1I GCAGC 2 cut(s) 420, 540
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 44
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 44
BsuRI GGCC 5 cut(s) 60, 381, 653, 761, 770
BtrI CACGTC 1 cut(s) 694
BtsCI GGATG 2 cut(s) 170, 651
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 192, 305, 516
CciI TCATGA 1 cut(s) 94
CfoI GCGC 3 cut(s) 494, 527, 662
Cfr10I RCCGGY 2 cut(s) 676, 761
Cfr13I GGNCC 4 cut(s) 58, 424, 546, 651
CseI GACGC 3 cut(s) 341, 574, 597
CsiI ACCWGGT 1 cut(s) 714
CviAII CATG 2 cut(s) 95, 550
DdeI CTNAG 2 cut(s) 175, 348
DinI GGCGCC 2 cut(s) 493, 661
DpnI GATC 2 cut(s) 46, 452
DpnII GATC 2 cut(s) 44, 450
EaeI YGGCCR 1 cut(s) 768
Eam1104I CTCTTC 2 cut(s) 117, 314
EarI CTCTTC 2 cut(s) 117, 314
EciI GGCGGA 2 cut(s) 202, 279
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 432, 654
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 2 cut(s) 424, 546
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 2 cut(s) 297, 431
EcoO109I RGGNCCY 2 cut(s) 424, 651
EcoRII CCWGG 2 cut(s) 464, 714
EcoT14I CCWWGG 2 cut(s) 432, 654
EcoT38I GRGCYC 1 cut(s) 396
EgeI GGCGCC 2 cut(s) 493, 661
EheI GGCGCC 2 cut(s) 493, 661
ErhI CCWWGG 2 cut(s) 432, 654
FaeI CATG 2 cut(s) 98, 553
FaiI YATR 7 cut(s) 53, 96, 164, 200, 299, 551, 710
FalI AAGNNNNNCTT 4 cut(s) 8, 40, 296, 328
FaqI GGGAC 2 cut(s) 482, 644
FatI CATG 2 cut(s) 94, 549
FauI CCCGC 1 cut(s) 162
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 5 cut(s) 61, 247, 409, 554, 663
FokI GGATG 2 cut(s) 157, 658
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 5 cut(s) 61, 247, 409, 554, 663
FspBI CTAG 5 cut(s) 279, 344, 368, 405, 443
GlaI GCGC 3 cut(s) 493, 526, 661
GluI GCNGC 5 cut(s) 61, 247, 409, 554, 663
HaeII RGCGCY 3 cut(s) 495, 528, 663
HaeIII GGCC 5 cut(s) 60, 381, 653, 761, 770
HapII CCGG 4 cut(s) 139, 327, 677, 762
HgaI GACGC 3 cut(s) 341, 574, 597
HhaI GCGC 3 cut(s) 494, 527, 662
Hin1I GRCGYC 3 cut(s) 492, 585, 660
Hin1II CATG 2 cut(s) 98, 553
Hin6I GCGC 3 cut(s) 492, 525, 660
HinP1I GCGC 3 cut(s) 492, 525, 660
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 1 cut(s) 570
HpaII CCGG 4 cut(s) 139, 327, 677, 762
HphI GGTGA 2 cut(s) 496, 701
Hpy166II GTNNAC 2 cut(s) 400, 697
Hpy188I TCNGA 3 cut(s) 126, 539, 590
Hpy188III TCNNGA 2 cut(s) 95, 448
Hpy8I GTNNAC 2 cut(s) 400, 697
Hpy99I CGWCG 6 cut(s) 123, 401, 458, 590, 613, 704
HpyAV CCTTC 4 cut(s) 397, 495, 680, 751
HpyCH4III ACNGT 3 cut(s) 230, 605, 779
HpyCH4IV ACGT 2 cut(s) 221, 693
HpyCH4V TGCA 1 cut(s) 567
HpyF10VI GCNNNNNNNGC 9 cut(s) 163, 191, 200, 524, 559, 591, 605, 659, 767
HpyF3I CTNAG 2 cut(s) 175, 348
HpySE526I ACGT 2 cut(s) 221, 693
Hsp92I GRCGYC 3 cut(s) 492, 585, 660
Hsp92II CATG 2 cut(s) 98, 553
HspAI GCGC 3 cut(s) 492, 525, 660
KasI GGCGCC 2 cut(s) 491, 659
Kzo9I GATC 2 cut(s) 44, 450
LguI GCTCTTC 2 cut(s) 117, 314
LmnI GCTCC 2 cut(s) 32, 287
Lsp1109I GCAGC 2 cut(s) 420, 540
MabI ACCWGGT 1 cut(s) 714
MaeI CTAG 5 cut(s) 279, 344, 368, 405, 443
MaeII ACGT 2 cut(s) 221, 693
MaeIII GTNAC 1 cut(s) 689
MalI GATC 2 cut(s) 46, 452
MboI GATC 2 cut(s) 44, 450
MboII GAAGA 6 cut(s) 104, 107, 301, 304, 347, 493
MfeI CAATTG 1 cut(s) 508
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 187, 396
MluCI AATT 2 cut(s) 143, 508
Mly113I GGCGCC 2 cut(s) 492, 660
MmeI TCCRAC 2 cut(s) 72, 568
MseI TTAA 1 cut(s) 27
MspI CCGG 4 cut(s) 139, 327, 677, 762
MspR9I CCNGG 2 cut(s) 466, 716
MunI CAATTG 1 cut(s) 508
MvaI CCWGG 2 cut(s) 466, 716
MvnI CGCG 2 cut(s) 63, 518
MwoI GCNNNNNNNGC 9 cut(s) 163, 191, 200, 524, 559, 591, 605, 659, 767
NarI GGCGCC 2 cut(s) 492, 660
NdeII GATC 2 cut(s) 44, 450
NlaIII CATG 2 cut(s) 98, 553
NlaIV GGNNCC 4 cut(s) 331, 493, 547, 661
NmeAIII GCCGAG 4 cut(s) 86, 553, 620, 691
NmuCI GTSAC 1 cut(s) 689
PagI TCATGA 1 cut(s) 94
PciSI GCTCTTC 2 cut(s) 117, 314
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 1 cut(s) 570
PflFI GACNNNGTC 1 cut(s) 716
PkrI GCNGC 5 cut(s) 62, 248, 410, 555, 664
PluTI GGCGCC 2 cut(s) 495, 663
PpuMI RGGWCCY 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 464, 714
PspGI CCWGG 2 cut(s) 464, 714
PspN4I GGNNCC 4 cut(s) 331, 493, 547, 661
PspPI GGNCC 4 cut(s) 58, 424, 546, 651
PspPPI RGGWCCY 1 cut(s) 424
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 716
SacI GAGCTC 1 cut(s) 396
SalI GTCGAC 1 cut(s) 398
SapI GCTCTTC 2 cut(s) 117, 314
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 5 cut(s) 61, 247, 409, 554, 663
Sau3AI GATC 2 cut(s) 44, 450
Sau96I GGNCC 4 cut(s) 58, 424, 546, 651
ScrFI CCNGG 2 cut(s) 466, 716
SduI GDGCHC 2 cut(s) 187, 396
SexAI ACCWGGT 1 cut(s) 714
SfiI GGCCNNNNNGGCC 1 cut(s) 767
SfoI GGCGCC 2 cut(s) 493, 661
SinI GGWCC 2 cut(s) 424, 546
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 143, 508
SsiI CCGC 9 cut(s) 61, 155, 213, 247, 290, 482, 518, 620, 663
SspDI GGCGCC 2 cut(s) 491, 659
SspMI CTAG 5 cut(s) 279, 344, 368, 405, 443
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 464, 714
StyI CCWWGG 2 cut(s) 432, 654
TaaI ACNGT 3 cut(s) 230, 605, 779
TaiI ACGT 2 cut(s) 224, 696
TaqI TCGA 4 cut(s) 147, 390, 399, 638
TasI AATT 2 cut(s) 143, 508
TauI GCSGC 3 cut(s) 63, 249, 665
TfiI GAWTC 1 cut(s) 570
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 569
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 2 cut(s) 408, 553
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 1 cut(s) 304
TspRI CASTG 1 cut(s) 569
Tth111I GACNNNGTC 1 cut(s) 716
VpaK11BI GGWCC 2 cut(s) 424, 546
XagI CCTNNNNNAGG 2 cut(s) 297, 431
XapI RAATTY 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 5 cut(s) 279, 344, 368, 405, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.