Rmu_sc0004902.1_g000001

40S ribosomal protein S21-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004902.1
N/A
Physical Location & Seq
Forward (+)
1 .. 3481
3481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
gctgcgacggacgctcaaatagggtcggacggaggccggaggcataccagacagcttctgggcagtgatcgaggttgcaggtttctaggcagcgctgcaaccacgtcgctggcgactccagcgactgcagatcggtcaatcctacccctggcctcagtcaggcaagccccgccgctctgtcgcgccccgagccgagctccagcgtggacgtccgtactttatcagtcctacccctggcctcagtcaggcaagccccgtcgctccgtcgcgcccctagccgagctccagcgtggacgtccgcactttagcgaaagtgcggacgtcctctttggaacgactccctttgagaagctcaggtttgtttgtgtttctgttgggtcaggatttgttgtgtttgcggaagtaatcaactcattgattaaaatgcagaacgaagagggtcaaatcactgagctctacattcccaggaaatgctcagcaactaacaggctgatcacctcgaaggatcatgcatctgttcagatcaacatcgggcatcttgatgagaatggcatctatactggccagttctccacttttgctctttgtggttatgtccgtgctcaggtgcagaggtgctccttcactcatttcagtacttgtgttcaaataacttggcgctttgaaatttttattgggatctctttcattttctacgctggcagaacgggaacggcctctgcgtgtccaattagatggaggtcttggtttgcctcttttggcaaagcagggattgctcatccaccacaatctccagaattccctatagtattttggggccgcttgaggtggatttccttcctggatttggggaagcaccttaggacaattggttctttaccatcagaaatcaccatcaggttcccctcatcttctcaatctgctgtttttgtggctgatctcatcaattggaacaaccaaacttatgatcttgggacctcctccaacctgaggaagtcgctgccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S21e

Protein Analysis

335

Amino Acids

37.0

Weight (kDa)

9.81

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21e PF01249 142 - 202 6.1e-30 Ribosomal protein S21e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 212, 298, 324
Acc36I ACCTGC 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 175
AccII CGCG 2 cut(s) 183, 269
AciI CCGC 6 cut(s) 170, 173, 299, 317, 398, 820
AclWI GGATC 2 cut(s) 513, 686
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 2 cut(s) 666, 797
AcyI GRCGYC 3 cut(s) 209, 295, 321
AfaI GTAC 2 cut(s) 216, 637
AfeI AGCGCT 1 cut(s) 94
AfiI CCNNNNNNNGG 7 cut(s) 148, 159, 234, 245, 604, 847, 990
AgsI TTSAA 2 cut(s) 647, 665
AjiI CACGTC 1 cut(s) 105
AjnI CCWGG 4 cut(s) 147, 233, 464, 840
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AloI GAACNNNNNNTCC 2 cut(s) 856, 888
AluBI AGCT 5 cut(s) 55, 197, 283, 352, 454
AluI AGCT 5 cut(s) 55, 197, 283, 352, 454
Alw21I GWGCWC 5 cut(s) 199, 285, 456, 604, 620
AlwI GGATC 2 cut(s) 513, 686
AlwNI CAGNNNCTG 2 cut(s) 58, 125
Ama87I CYCGRG 1 cut(s) 187
Aor51HI AGCGCT 1 cut(s) 94
AoxI GGCC 6 cut(s) 34, 150, 236, 562, 714, 817
ApeKI GCWGC 4 cut(s) 2, 90, 95, 1000
ApoI RAATTY 2 cut(s) 666, 797
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspLEI GCGC 4 cut(s) 95, 185, 271, 660
AspS9I GGNCC 2 cut(s) 817, 975
AsuHPI GGTGA 2 cut(s) 487, 883
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 1 cut(s) 975
AxyI CCTNAGG 2 cut(s) 860, 989
BalI TGGCCA 1 cut(s) 564
BanII GRGCYC 3 cut(s) 199, 285, 456
Bbv12I GWGCWC 5 cut(s) 199, 285, 456, 604, 620
BbvI GCAGC 3 cut(s) 82, 102, 987
BccI CCATC 3 cut(s) 729, 889, 902
BceAI ACGGC 1 cut(s) 729
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BciT130I CCWGG 4 cut(s) 149, 235, 466, 842
BclI TGATCA 1 cut(s) 492
BfaI CTAG 2 cut(s) 86, 275
BfmI CTRYAG 2 cut(s) 126, 804
BfoI RGCGCY 2 cut(s) 96, 661
BfuAI ACCTGC 1 cut(s) 69
BisI GCNGC 6 cut(s) 3, 91, 96, 173, 820, 1001
BlpI GCTNAGC 1 cut(s) 475
BlsI GCNGC 6 cut(s) 4, 92, 97, 174, 821, 1002
BmcAI AGTACT 1 cut(s) 637
Bme1390I CCNGG 4 cut(s) 149, 235, 466, 842
Bme18I GGWCC 1 cut(s) 975
BmeT110I CYCGRG 1 cut(s) 187
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 817, 975
BmiI GGNNCC 3 cut(s) 818, 902, 976
BmrFI CCNGG 4 cut(s) 149, 235, 466, 842
BmsI GCATC 3 cut(s) 521, 544, 561
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BpmI CTGGAG 4 cut(s) 102, 183, 269, 777
Bpu10I CCTNAGC 2 cut(s) 353, 603
Bpu1102I GCTNAGC 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 527
BsaHI GRCGYC 3 cut(s) 209, 295, 321
BsaJI CCNNGG 3 cut(s) 147, 233, 464
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 159, 234, 245, 604, 847, 990
Bse1I ACTGG 2 cut(s) 565, 565
Bse21I CCTNAGG 2 cut(s) 860, 989
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 4 cut(s) 149, 235, 466, 842
BseDI CCNNGG 3 cut(s) 147, 233, 464
BseGI GGATG 1 cut(s) 778
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 7 cut(s) 148, 159, 234, 245, 604, 847, 990
BseMII CTCAG 7 cut(s) 168, 254, 367, 441, 489, 617, 980
BseNI ACTGG 2 cut(s) 565, 565
BseRI GAGGAG 1 cut(s) 970
BseXI GCAGC 3 cut(s) 82, 102, 987
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 2 cut(s) 183, 269
BshFI GGCC 6 cut(s) 36, 152, 238, 564, 716, 819
BsiHKAI GWGCWC 5 cut(s) 199, 285, 456, 604, 620
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 1 cut(s) 37
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 7 cut(s) 148, 159, 234, 245, 604, 847, 990
BsmFI GGGAC 1 cut(s) 988
BsnI GGCC 6 cut(s) 36, 152, 238, 564, 716, 819
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 5 cut(s) 199, 285, 456, 604, 620
Bsp143I GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
Bsp1720I GCTNAGC 1 cut(s) 475
BspACI CCGC 6 cut(s) 170, 173, 299, 317, 398, 820
BspANI GGCC 6 cut(s) 36, 152, 238, 564, 716, 819
BspCNI CTCAG 7 cut(s) 167, 253, 366, 442, 488, 616, 981
BspFNI CGCG 2 cut(s) 183, 269
BspLI GGNNCC 3 cut(s) 818, 902, 976
BspMAI CTGCAG 1 cut(s) 130
BspMI ACCTGC 1 cut(s) 69
BspPI GGATC 2 cut(s) 513, 686
BsrBI CCGCTC 1 cut(s) 175
BsrI ACTGG 2 cut(s) 565, 565
BssECI CCNNGG 3 cut(s) 147, 233, 464
BssMI GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
BssNI GRCGYC 3 cut(s) 209, 295, 321
Bst2UI CCWGG 4 cut(s) 149, 235, 466, 842
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 3 cut(s) 209, 295, 321
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 4 cut(s) 111, 165, 251, 700
BstDEI CTNAG 8 cut(s) 154, 240, 353, 450, 475, 603, 860, 989
BstENI CCTNNNNNAGG 2 cut(s) 157, 243
BstF5I GGATG 1 cut(s) 778
BstFNI CGCG 2 cut(s) 183, 269
BstH2I RGCGCY 2 cut(s) 96, 661
BstHHI GCGC 4 cut(s) 95, 185, 271, 660
BstKTI GATC 8 cut(s) 70, 133, 495, 508, 525, 681, 940, 970
BstMBI GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
BstMWI GCNNNNNNNGC 6 cut(s) 11, 119, 169, 189, 275, 773
BstNI CCWGG 4 cut(s) 149, 235, 466, 842
BstSCI CCNGG 4 cut(s) 147, 233, 464, 840
BstSFI CTRYAG 2 cut(s) 126, 804
BstUI CGCG 2 cut(s) 183, 269
BstV1I GCAGC 3 cut(s) 82, 102, 987
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 2 cut(s) 109, 735
BstYI RGATCY 1 cut(s) 678
Bsu36I CCTNAGG 2 cut(s) 860, 989
BsuRI GGCC 6 cut(s) 36, 152, 238, 564, 716, 819
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 1 cut(s) 778
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 447
BveI ACCTGC 1 cut(s) 69
Cac8I GCNNGC 4 cut(s) 111, 165, 251, 700
CaiI CAGNNNCTG 2 cut(s) 58, 125
CfoI GCGC 4 cut(s) 95, 185, 271, 660
Cfr13I GGNCC 2 cut(s) 817, 975
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 215, 636
CviAII CATG 1 cut(s) 509
CviQI GTAC 2 cut(s) 215, 636
DdeI CTNAG 8 cut(s) 154, 240, 353, 450, 475, 603, 860, 989
DpnI GATC 8 cut(s) 69, 132, 494, 507, 524, 680, 939, 969
DpnII GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 3 cut(s) 197, 283, 454
Eco24I GRGCYC 3 cut(s) 199, 285, 456
Eco47I GGWCC 1 cut(s) 975
Eco47III AGCGCT 1 cut(s) 94
Eco53kI GAGCTC 3 cut(s) 197, 283, 454
Eco81I CCTNAGG 2 cut(s) 860, 989
Eco88I CYCGRG 1 cut(s) 187
EcoICRI GAGCTC 3 cut(s) 197, 283, 454
EcoNI CCTNNNNNAGG 2 cut(s) 157, 243
EcoO109I RGGNCCY 1 cut(s) 975
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 4 cut(s) 147, 233, 464, 840
EcoT22I ATGCAT 1 cut(s) 514
EcoT38I GRGCYC 3 cut(s) 199, 285, 456
FaeI CATG 1 cut(s) 512
FaiI YATR 7 cut(s) 45, 510, 558, 594, 806, 966, 1006
FaqI GGGAC 1 cut(s) 988
FatI CATG 1 cut(s) 508
FauI CCCGC 1 cut(s) 177
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 6 cut(s) 3, 91, 96, 173, 820, 1001
FokI GGATG 1 cut(s) 765
FriOI GRGCYC 3 cut(s) 199, 285, 456
Fsp4HI GCNGC 6 cut(s) 3, 91, 96, 173, 820, 1001
FspBI CTAG 2 cut(s) 86, 275
GlaI GCGC 4 cut(s) 94, 184, 270, 659
GluI GCNGC 6 cut(s) 3, 91, 96, 173, 820, 1001
GsuI CTGGAG 4 cut(s) 102, 183, 269, 777
HaeII RGCGCY 2 cut(s) 96, 661
HaeIII GGCC 6 cut(s) 36, 152, 238, 564, 716, 819
HapII CCGG 1 cut(s) 37
HgaI GACGC 1 cut(s) 20
HhaI GCGC 4 cut(s) 95, 185, 271, 660
Hin1I GRCGYC 3 cut(s) 209, 295, 321
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 4 cut(s) 93, 183, 269, 658
HinP1I GCGC 4 cut(s) 93, 183, 269, 658
HinfI GANTC 2 cut(s) 115, 337
HpaII CCGG 1 cut(s) 37
HphI GGTGA 2 cut(s) 487, 883
Hpy166II GTNNAC 2 cut(s) 207, 293
Hpy188I TCNGA 3 cut(s) 28, 522, 886
Hpy188III TCNNGA 3 cut(s) 381, 539, 794
Hpy8I GTNNAC 2 cut(s) 207, 293
Hpy99I CGWCG 4 cut(s) 10, 109, 261, 269
HpyAV CCTTC 3 cut(s) 496, 631, 847
HpyCH4IV ACGT 4 cut(s) 104, 209, 295, 321
HpyCH4V TGCA 6 cut(s) 78, 98, 128, 427, 512, 610
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 119, 169, 189, 275, 773
HpyF3I CTNAG 8 cut(s) 154, 240, 353, 450, 475, 603, 860, 989
HpySE526I ACGT 4 cut(s) 104, 209, 295, 321
Hsp92I GRCGYC 3 cut(s) 209, 295, 321
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 4 cut(s) 93, 183, 269, 658
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
LmnI GCTCC 4 cut(s) 202, 266, 288, 623
Lsp1109I GCAGC 3 cut(s) 82, 102, 987
LweI GCATC 3 cut(s) 521, 544, 561
MaeI CTAG 2 cut(s) 86, 275
MaeII ACGT 4 cut(s) 104, 209, 295, 321
MalI GATC 8 cut(s) 69, 132, 494, 507, 524, 680, 939, 969
MbiI CCGCTC 1 cut(s) 175
MboI GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
MboII GAAGA 2 cut(s) 446, 903
MfeI CAATTG 2 cut(s) 867, 946
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 5 cut(s) 199, 285, 456, 604, 620
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 5 cut(s) 666, 729, 797, 867, 946
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 2 cut(s) 109, 331
MmeI TCCRAC 2 cut(s) 6, 1008
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 514
MscI TGGCCA 1 cut(s) 564
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 564
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 4 cut(s) 149, 235, 466, 842
MunI CAATTG 2 cut(s) 867, 946
MvaI CCWGG 4 cut(s) 149, 235, 466, 842
MvnI CGCG 2 cut(s) 183, 269
MwoI GCNNNNNNNGC 6 cut(s) 11, 119, 169, 189, 275, 773
NdeII GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 3 cut(s) 818, 902, 976
NmeAIII GCCGAG 2 cut(s) 218, 304
NsiI ATGCAT 1 cut(s) 514
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 719
PfoI TCCNGGA 1 cut(s) 840
PkrI GCNGC 6 cut(s) 4, 92, 97, 174, 821, 1002
PleI GAGTC 2 cut(s) 109, 331
PpsI GAGTC 2 cut(s) 109, 331
PpuMI RGGWCCY 1 cut(s) 975
Psp124BI GAGCTC 3 cut(s) 199, 285, 456
Psp5II RGGWCCY 1 cut(s) 975
Psp6I CCWGG 4 cut(s) 147, 233, 464, 840
PspGI CCWGG 4 cut(s) 147, 233, 464, 840
PspN4I GGNNCC 3 cut(s) 818, 902, 976
PspPI GGNCC 2 cut(s) 817, 975
PspPPI RGGWCCY 1 cut(s) 975
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 2 cut(s) 58, 125
PsuI RGATCY 1 cut(s) 678
RsaI GTAC 2 cut(s) 216, 637
RsaNI GTAC 2 cut(s) 215, 636
RseI CAYNNNNRTG 1 cut(s) 540
SacI GAGCTC 3 cut(s) 199, 285, 456
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 6 cut(s) 3, 91, 96, 173, 820, 1001
Sau3AI GATC 8 cut(s) 67, 130, 492, 505, 522, 678, 937, 967
Sau96I GGNCC 2 cut(s) 817, 975
ScaI AGTACT 1 cut(s) 637
SchI GAGTC 2 cut(s) 109, 331
ScrFI CCNGG 4 cut(s) 149, 235, 466, 842
SduI GDGCHC 5 cut(s) 199, 285, 456, 604, 620
SfaNI GCATC 3 cut(s) 521, 544, 561
SfcI CTRYAG 2 cut(s) 126, 804
SinI GGWCC 1 cut(s) 975
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 5 cut(s) 666, 729, 797, 867, 946
SsiI CCGC 6 cut(s) 170, 173, 299, 317, 398, 820
SspMI CTAG 2 cut(s) 86, 275
SstI GAGCTC 3 cut(s) 199, 285, 456
StyD4I CCNGG 4 cut(s) 147, 233, 464, 840
TaiI ACGT 4 cut(s) 107, 212, 298, 324
TaqI TCGA 2 cut(s) 70, 500
TaqII GACCGA 1 cut(s) 123
TasI AATT 5 cut(s) 666, 729, 797, 867, 946
TatI WGTACW 1 cut(s) 635
TauI GCSGC 2 cut(s) 175, 822
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 2 cut(s) 70, 454
TseI GCWGC 4 cut(s) 2, 90, 95, 1000
TspDTI ATGAA 1 cut(s) 676
TspGWI ACGGA 5 cut(s) 23, 45, 202, 253, 587
TspRI CASTG 2 cut(s) 70, 454
VpaK11BI GGWCC 1 cut(s) 975
XagI CCTNNNNNAGG 2 cut(s) 157, 243
XapI RAATTY 2 cut(s) 666, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XspI CTAG 2 cut(s) 86, 275
ZraI GACGTC 3 cut(s) 210, 296, 322
ZrmI AGTACT 1 cut(s) 637
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.